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RStudio rstudio ide 1.0.143
Rstudio Ide 1.0.143, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Virus:

Article Title: The effect of vortioxetine on faecal microbiota in high-fat diet-exposed mice-A link to weight protection.
Article Snippet: Funding information Axel Muusfeldt fonden; Region Midtjylland Abstract Objective: Weight gain is a common side effect of antidepressive treatment, causing distress among patients and caretakers as it can lead to treatment discontinuation and complications such as diabetes type II and cardiovascular disease.. Vortioxetine is one of the newer antidepressants and the pharmacodynamics differ from the selective serotonin reuptake inhibitors.. It is marketed as being weight neutral; however, there is little evidence as to why.

Article Title: Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures
Article Snippet: R Studio Desktop IDE 1.0.143 , RStudio , https://rstudio.com/.

Article Title: Quality of life in idiopathic pulmonary fibrosis in Latin American countries.
Article Snippet: Data were collected in Excel and analyzed with Stata 13.1 (Stata Corp LP, College Station, TX, USA) and R Studio IDE 1.4.1103.

Article Title: Distinct Immunological Landscapes Characterize Inherited and Sporadic Mismatch Repair Deficient Endometrial Cancer
Article Snippet: Statistical analyses were conducted in R 3.4.0 × 64/RStudio IDE 1.0.143.

Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Desktop IDE 1.0.143 RStudio https://www.rstudio.com Bioconductor R packages 3.5 Bioconductor http://bioconductor.org Tidyverse R packages 1.1.1 CRAN https://cran.r-project.org/web/packages/tidyverse/index.html ImageJ National Institutes of Health https://imagej.nih.gov/ij/ SnapGene Viewer SnapGene www.snapgene.com/products/snapgene_viewer/ Li-COR Odyssey N/A https://www.licor.com/bio/products/imaging_systems/odyssey Other Catalogue of Somatic Mutations in Cancer v80; Cancer gene census tumor suppressor annotations April 2017 Forbes et al., 2017 http://cancer.sanger.ac.uk/cosmic Tumor suppressor predictions Tokheim et al., 2016 http://www.pnas.org/content/suppl/2016/11/22/1616440113.DCSupplemental/pnas.1616440113.sd04.xlsx Open in a separate window KEY RESOURCES TABLE

Article Title: Recruitment enhancement varies by taxonomic group and oyster reef habitat characteristics.
Article Snippet: All analyses were conducted in R 3.6.1 (R Development Core Team, 2019) on the RStudio IDE 1.2.1335 (RStudio Team, 2019).

Subcloning:

Article Title: The effect of vortioxetine on faecal microbiota in high-fat diet-exposed mice-A link to weight protection.
Article Snippet: Funding information Axel Muusfeldt fonden; Region Midtjylland Abstract Objective: Weight gain is a common side effect of antidepressive treatment, causing distress among patients and caretakers as it can lead to treatment discontinuation and complications such as diabetes type II and cardiovascular disease.. Vortioxetine is one of the newer antidepressants and the pharmacodynamics differ from the selective serotonin reuptake inhibitors.. It is marketed as being weight neutral; however, there is little evidence as to why.

Article Title: Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures
Article Snippet: R Studio Desktop IDE 1.0.143 , RStudio , https://rstudio.com/.

Article Title: Quality of life in idiopathic pulmonary fibrosis in Latin American countries.
Article Snippet: Data were collected in Excel and analyzed with Stata 13.1 (Stata Corp LP, College Station, TX, USA) and R Studio IDE 1.4.1103.

Article Title: Distinct Immunological Landscapes Characterize Inherited and Sporadic Mismatch Repair Deficient Endometrial Cancer
Article Snippet: Statistical analyses were conducted in R 3.4.0 × 64/RStudio IDE 1.0.143.

Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Desktop IDE 1.0.143 RStudio https://www.rstudio.com Bioconductor R packages 3.5 Bioconductor http://bioconductor.org Tidyverse R packages 1.1.1 CRAN https://cran.r-project.org/web/packages/tidyverse/index.html ImageJ National Institutes of Health https://imagej.nih.gov/ij/ SnapGene Viewer SnapGene www.snapgene.com/products/snapgene_viewer/ Li-COR Odyssey N/A https://www.licor.com/bio/products/imaging_systems/odyssey Other Catalogue of Somatic Mutations in Cancer v80; Cancer gene census tumor suppressor annotations April 2017 Forbes et al., 2017 http://cancer.sanger.ac.uk/cosmic Tumor suppressor predictions Tokheim et al., 2016 http://www.pnas.org/content/suppl/2016/11/22/1616440113.DCSupplemental/pnas.1616440113.sd04.xlsx Open in a separate window KEY RESOURCES TABLE

Article Title: Recruitment enhancement varies by taxonomic group and oyster reef habitat characteristics.
Article Snippet: All analyses were conducted in R 3.6.1 (R Development Core Team, 2019) on the RStudio IDE 1.2.1335 (RStudio Team, 2019).

Recombinant:

Article Title: The effect of vortioxetine on faecal microbiota in high-fat diet-exposed mice-A link to weight protection.
Article Snippet: Funding information Axel Muusfeldt fonden; Region Midtjylland Abstract Objective: Weight gain is a common side effect of antidepressive treatment, causing distress among patients and caretakers as it can lead to treatment discontinuation and complications such as diabetes type II and cardiovascular disease.. Vortioxetine is one of the newer antidepressants and the pharmacodynamics differ from the selective serotonin reuptake inhibitors.. It is marketed as being weight neutral; however, there is little evidence as to why.

Article Title: Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures
Article Snippet: R Studio Desktop IDE 1.0.143 , RStudio , https://rstudio.com/.

Article Title: Quality of life in idiopathic pulmonary fibrosis in Latin American countries.
Article Snippet: Data were collected in Excel and analyzed with Stata 13.1 (Stata Corp LP, College Station, TX, USA) and R Studio IDE 1.4.1103.

Article Title: Distinct Immunological Landscapes Characterize Inherited and Sporadic Mismatch Repair Deficient Endometrial Cancer
Article Snippet: Statistical analyses were conducted in R 3.4.0 × 64/RStudio IDE 1.0.143.

Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Desktop IDE 1.0.143 RStudio https://www.rstudio.com Bioconductor R packages 3.5 Bioconductor http://bioconductor.org Tidyverse R packages 1.1.1 CRAN https://cran.r-project.org/web/packages/tidyverse/index.html ImageJ National Institutes of Health https://imagej.nih.gov/ij/ SnapGene Viewer SnapGene www.snapgene.com/products/snapgene_viewer/ Li-COR Odyssey N/A https://www.licor.com/bio/products/imaging_systems/odyssey Other Catalogue of Somatic Mutations in Cancer v80; Cancer gene census tumor suppressor annotations April 2017 Forbes et al., 2017 http://cancer.sanger.ac.uk/cosmic Tumor suppressor predictions Tokheim et al., 2016 http://www.pnas.org/content/suppl/2016/11/22/1616440113.DCSupplemental/pnas.1616440113.sd04.xlsx Open in a separate window KEY RESOURCES TABLE

Article Title: Recruitment enhancement varies by taxonomic group and oyster reef habitat characteristics.
Article Snippet: All analyses were conducted in R 3.6.1 (R Development Core Team, 2019) on the RStudio IDE 1.2.1335 (RStudio Team, 2019).

Staining:

Article Title: The effect of vortioxetine on faecal microbiota in high-fat diet-exposed mice-A link to weight protection.
Article Snippet: Funding information Axel Muusfeldt fonden; Region Midtjylland Abstract Objective: Weight gain is a common side effect of antidepressive treatment, causing distress among patients and caretakers as it can lead to treatment discontinuation and complications such as diabetes type II and cardiovascular disease.. Vortioxetine is one of the newer antidepressants and the pharmacodynamics differ from the selective serotonin reuptake inhibitors.. It is marketed as being weight neutral; however, there is little evidence as to why.

Article Title: Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures
Article Snippet: R Studio Desktop IDE 1.0.143 , RStudio , https://rstudio.com/.

Article Title: Quality of life in idiopathic pulmonary fibrosis in Latin American countries.
Article Snippet: Data were collected in Excel and analyzed with Stata 13.1 (Stata Corp LP, College Station, TX, USA) and R Studio IDE 1.4.1103.

Article Title: Distinct Immunological Landscapes Characterize Inherited and Sporadic Mismatch Repair Deficient Endometrial Cancer
Article Snippet: Statistical analyses were conducted in R 3.4.0 × 64/RStudio IDE 1.0.143.

Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Desktop IDE 1.0.143 RStudio https://www.rstudio.com Bioconductor R packages 3.5 Bioconductor http://bioconductor.org Tidyverse R packages 1.1.1 CRAN https://cran.r-project.org/web/packages/tidyverse/index.html ImageJ National Institutes of Health https://imagej.nih.gov/ij/ SnapGene Viewer SnapGene www.snapgene.com/products/snapgene_viewer/ Li-COR Odyssey N/A https://www.licor.com/bio/products/imaging_systems/odyssey Other Catalogue of Somatic Mutations in Cancer v80; Cancer gene census tumor suppressor annotations April 2017 Forbes et al., 2017 http://cancer.sanger.ac.uk/cosmic Tumor suppressor predictions Tokheim et al., 2016 http://www.pnas.org/content/suppl/2016/11/22/1616440113.DCSupplemental/pnas.1616440113.sd04.xlsx Open in a separate window KEY RESOURCES TABLE

Article Title: Recruitment enhancement varies by taxonomic group and oyster reef habitat characteristics.
Article Snippet: All analyses were conducted in R 3.6.1 (R Development Core Team, 2019) on the RStudio IDE 1.2.1335 (RStudio Team, 2019).

Magnetic Beads:

Article Title: The effect of vortioxetine on faecal microbiota in high-fat diet-exposed mice-A link to weight protection.
Article Snippet: Funding information Axel Muusfeldt fonden; Region Midtjylland Abstract Objective: Weight gain is a common side effect of antidepressive treatment, causing distress among patients and caretakers as it can lead to treatment discontinuation and complications such as diabetes type II and cardiovascular disease.. Vortioxetine is one of the newer antidepressants and the pharmacodynamics differ from the selective serotonin reuptake inhibitors.. It is marketed as being weight neutral; however, there is little evidence as to why.

Article Title: Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures
Article Snippet: R Studio Desktop IDE 1.0.143 , RStudio , https://rstudio.com/.

Article Title: Quality of life in idiopathic pulmonary fibrosis in Latin American countries.
Article Snippet: Data were collected in Excel and analyzed with Stata 13.1 (Stata Corp LP, College Station, TX, USA) and R Studio IDE 1.4.1103.

Article Title: Distinct Immunological Landscapes Characterize Inherited and Sporadic Mismatch Repair Deficient Endometrial Cancer
Article Snippet: Statistical analyses were conducted in R 3.4.0 × 64/RStudio IDE 1.0.143.

Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Desktop IDE 1.0.143 RStudio https://www.rstudio.com Bioconductor R packages 3.5 Bioconductor http://bioconductor.org Tidyverse R packages 1.1.1 CRAN https://cran.r-project.org/web/packages/tidyverse/index.html ImageJ National Institutes of Health https://imagej.nih.gov/ij/ SnapGene Viewer SnapGene www.snapgene.com/products/snapgene_viewer/ Li-COR Odyssey N/A https://www.licor.com/bio/products/imaging_systems/odyssey Other Catalogue of Somatic Mutations in Cancer v80; Cancer gene census tumor suppressor annotations April 2017 Forbes et al., 2017 http://cancer.sanger.ac.uk/cosmic Tumor suppressor predictions Tokheim et al., 2016 http://www.pnas.org/content/suppl/2016/11/22/1616440113.DCSupplemental/pnas.1616440113.sd04.xlsx Open in a separate window KEY RESOURCES TABLE

Article Title: Recruitment enhancement varies by taxonomic group and oyster reef habitat characteristics.
Article Snippet: All analyses were conducted in R 3.6.1 (R Development Core Team, 2019) on the RStudio IDE 1.2.1335 (RStudio Team, 2019).

DNA Extraction:

Article Title: The effect of vortioxetine on faecal microbiota in high-fat diet-exposed mice-A link to weight protection.
Article Snippet: Funding information Axel Muusfeldt fonden; Region Midtjylland Abstract Objective: Weight gain is a common side effect of antidepressive treatment, causing distress among patients and caretakers as it can lead to treatment discontinuation and complications such as diabetes type II and cardiovascular disease.. Vortioxetine is one of the newer antidepressants and the pharmacodynamics differ from the selective serotonin reuptake inhibitors.. It is marketed as being weight neutral; however, there is little evidence as to why.

Article Title: Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures
Article Snippet: R Studio Desktop IDE 1.0.143 , RStudio , https://rstudio.com/.

Article Title: Quality of life in idiopathic pulmonary fibrosis in Latin American countries.
Article Snippet: Data were collected in Excel and analyzed with Stata 13.1 (Stata Corp LP, College Station, TX, USA) and R Studio IDE 1.4.1103.

Article Title: Distinct Immunological Landscapes Characterize Inherited and Sporadic Mismatch Repair Deficient Endometrial Cancer
Article Snippet: Statistical analyses were conducted in R 3.4.0 × 64/RStudio IDE 1.0.143.

Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Desktop IDE 1.0.143 RStudio https://www.rstudio.com Bioconductor R packages 3.5 Bioconductor http://bioconductor.org Tidyverse R packages 1.1.1 CRAN https://cran.r-project.org/web/packages/tidyverse/index.html ImageJ National Institutes of Health https://imagej.nih.gov/ij/ SnapGene Viewer SnapGene www.snapgene.com/products/snapgene_viewer/ Li-COR Odyssey N/A https://www.licor.com/bio/products/imaging_systems/odyssey Other Catalogue of Somatic Mutations in Cancer v80; Cancer gene census tumor suppressor annotations April 2017 Forbes et al., 2017 http://cancer.sanger.ac.uk/cosmic Tumor suppressor predictions Tokheim et al., 2016 http://www.pnas.org/content/suppl/2016/11/22/1616440113.DCSupplemental/pnas.1616440113.sd04.xlsx Open in a separate window KEY RESOURCES TABLE

Article Title: Recruitment enhancement varies by taxonomic group and oyster reef habitat characteristics.
Article Snippet: All analyses were conducted in R 3.6.1 (R Development Core Team, 2019) on the RStudio IDE 1.2.1335 (RStudio Team, 2019).

PCR Cloning:

Article Title: The effect of vortioxetine on faecal microbiota in high-fat diet-exposed mice-A link to weight protection.
Article Snippet: Funding information Axel Muusfeldt fonden; Region Midtjylland Abstract Objective: Weight gain is a common side effect of antidepressive treatment, causing distress among patients and caretakers as it can lead to treatment discontinuation and complications such as diabetes type II and cardiovascular disease.. Vortioxetine is one of the newer antidepressants and the pharmacodynamics differ from the selective serotonin reuptake inhibitors.. It is marketed as being weight neutral; however, there is little evidence as to why.

Article Title: Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures
Article Snippet: R Studio Desktop IDE 1.0.143 , RStudio , https://rstudio.com/.

Article Title: Quality of life in idiopathic pulmonary fibrosis in Latin American countries.
Article Snippet: Data were collected in Excel and analyzed with Stata 13.1 (Stata Corp LP, College Station, TX, USA) and R Studio IDE 1.4.1103.

Article Title: Distinct Immunological Landscapes Characterize Inherited and Sporadic Mismatch Repair Deficient Endometrial Cancer
Article Snippet: Statistical analyses were conducted in R 3.4.0 × 64/RStudio IDE 1.0.143.

Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Desktop IDE 1.0.143 RStudio https://www.rstudio.com Bioconductor R packages 3.5 Bioconductor http://bioconductor.org Tidyverse R packages 1.1.1 CRAN https://cran.r-project.org/web/packages/tidyverse/index.html ImageJ National Institutes of Health https://imagej.nih.gov/ij/ SnapGene Viewer SnapGene www.snapgene.com/products/snapgene_viewer/ Li-COR Odyssey N/A https://www.licor.com/bio/products/imaging_systems/odyssey Other Catalogue of Somatic Mutations in Cancer v80; Cancer gene census tumor suppressor annotations April 2017 Forbes et al., 2017 http://cancer.sanger.ac.uk/cosmic Tumor suppressor predictions Tokheim et al., 2016 http://www.pnas.org/content/suppl/2016/11/22/1616440113.DCSupplemental/pnas.1616440113.sd04.xlsx Open in a separate window KEY RESOURCES TABLE

Article Title: Recruitment enhancement varies by taxonomic group and oyster reef habitat characteristics.
Article Snippet: All analyses were conducted in R 3.6.1 (R Development Core Team, 2019) on the RStudio IDE 1.2.1335 (RStudio Team, 2019).

Sequencing:

Article Title: The effect of vortioxetine on faecal microbiota in high-fat diet-exposed mice-A link to weight protection.
Article Snippet: Funding information Axel Muusfeldt fonden; Region Midtjylland Abstract Objective: Weight gain is a common side effect of antidepressive treatment, causing distress among patients and caretakers as it can lead to treatment discontinuation and complications such as diabetes type II and cardiovascular disease.. Vortioxetine is one of the newer antidepressants and the pharmacodynamics differ from the selective serotonin reuptake inhibitors.. It is marketed as being weight neutral; however, there is little evidence as to why.

Article Title: Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures
Article Snippet: R Studio Desktop IDE 1.0.143 , RStudio , https://rstudio.com/.

Article Title: Quality of life in idiopathic pulmonary fibrosis in Latin American countries.
Article Snippet: Data were collected in Excel and analyzed with Stata 13.1 (Stata Corp LP, College Station, TX, USA) and R Studio IDE 1.4.1103.

Article Title: Distinct Immunological Landscapes Characterize Inherited and Sporadic Mismatch Repair Deficient Endometrial Cancer
Article Snippet: Statistical analyses were conducted in R 3.4.0 × 64/RStudio IDE 1.0.143.

Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Desktop IDE 1.0.143 RStudio https://www.rstudio.com Bioconductor R packages 3.5 Bioconductor http://bioconductor.org Tidyverse R packages 1.1.1 CRAN https://cran.r-project.org/web/packages/tidyverse/index.html ImageJ National Institutes of Health https://imagej.nih.gov/ij/ SnapGene Viewer SnapGene www.snapgene.com/products/snapgene_viewer/ Li-COR Odyssey N/A https://www.licor.com/bio/products/imaging_systems/odyssey Other Catalogue of Somatic Mutations in Cancer v80; Cancer gene census tumor suppressor annotations April 2017 Forbes et al., 2017 http://cancer.sanger.ac.uk/cosmic Tumor suppressor predictions Tokheim et al., 2016 http://www.pnas.org/content/suppl/2016/11/22/1616440113.DCSupplemental/pnas.1616440113.sd04.xlsx Open in a separate window KEY RESOURCES TABLE

Article Title: Recruitment enhancement varies by taxonomic group and oyster reef habitat characteristics.
Article Snippet: All analyses were conducted in R 3.6.1 (R Development Core Team, 2019) on the RStudio IDE 1.2.1335 (RStudio Team, 2019).

Cloning:

Article Title: The effect of vortioxetine on faecal microbiota in high-fat diet-exposed mice-A link to weight protection.
Article Snippet: Funding information Axel Muusfeldt fonden; Region Midtjylland Abstract Objective: Weight gain is a common side effect of antidepressive treatment, causing distress among patients and caretakers as it can lead to treatment discontinuation and complications such as diabetes type II and cardiovascular disease.. Vortioxetine is one of the newer antidepressants and the pharmacodynamics differ from the selective serotonin reuptake inhibitors.. It is marketed as being weight neutral; however, there is little evidence as to why.

Article Title: Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures
Article Snippet: R Studio Desktop IDE 1.0.143 , RStudio , https://rstudio.com/.

Article Title: Quality of life in idiopathic pulmonary fibrosis in Latin American countries.
Article Snippet: Data were collected in Excel and analyzed with Stata 13.1 (Stata Corp LP, College Station, TX, USA) and R Studio IDE 1.4.1103.

Article Title: Distinct Immunological Landscapes Characterize Inherited and Sporadic Mismatch Repair Deficient Endometrial Cancer
Article Snippet: Statistical analyses were conducted in R 3.4.0 × 64/RStudio IDE 1.0.143.

Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Desktop IDE 1.0.143 RStudio https://www.rstudio.com Bioconductor R packages 3.5 Bioconductor http://bioconductor.org Tidyverse R packages 1.1.1 CRAN https://cran.r-project.org/web/packages/tidyverse/index.html ImageJ National Institutes of Health https://imagej.nih.gov/ij/ SnapGene Viewer SnapGene www.snapgene.com/products/snapgene_viewer/ Li-COR Odyssey N/A https://www.licor.com/bio/products/imaging_systems/odyssey Other Catalogue of Somatic Mutations in Cancer v80; Cancer gene census tumor suppressor annotations April 2017 Forbes et al., 2017 http://cancer.sanger.ac.uk/cosmic Tumor suppressor predictions Tokheim et al., 2016 http://www.pnas.org/content/suppl/2016/11/22/1616440113.DCSupplemental/pnas.1616440113.sd04.xlsx Open in a separate window KEY RESOURCES TABLE

Article Title: Recruitment enhancement varies by taxonomic group and oyster reef habitat characteristics.
Article Snippet: All analyses were conducted in R 3.6.1 (R Development Core Team, 2019) on the RStudio IDE 1.2.1335 (RStudio Team, 2019).

Plasmid Preparation:

Article Title: The effect of vortioxetine on faecal microbiota in high-fat diet-exposed mice-A link to weight protection.
Article Snippet: Funding information Axel Muusfeldt fonden; Region Midtjylland Abstract Objective: Weight gain is a common side effect of antidepressive treatment, causing distress among patients and caretakers as it can lead to treatment discontinuation and complications such as diabetes type II and cardiovascular disease.. Vortioxetine is one of the newer antidepressants and the pharmacodynamics differ from the selective serotonin reuptake inhibitors.. It is marketed as being weight neutral; however, there is little evidence as to why.

Article Title: Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures
Article Snippet: R Studio Desktop IDE 1.0.143 , RStudio , https://rstudio.com/.

Article Title: Quality of life in idiopathic pulmonary fibrosis in Latin American countries.
Article Snippet: Data were collected in Excel and analyzed with Stata 13.1 (Stata Corp LP, College Station, TX, USA) and R Studio IDE 1.4.1103.

Article Title: Distinct Immunological Landscapes Characterize Inherited and Sporadic Mismatch Repair Deficient Endometrial Cancer
Article Snippet: Statistical analyses were conducted in R 3.4.0 × 64/RStudio IDE 1.0.143.

Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Desktop IDE 1.0.143 RStudio https://www.rstudio.com Bioconductor R packages 3.5 Bioconductor http://bioconductor.org Tidyverse R packages 1.1.1 CRAN https://cran.r-project.org/web/packages/tidyverse/index.html ImageJ National Institutes of Health https://imagej.nih.gov/ij/ SnapGene Viewer SnapGene www.snapgene.com/products/snapgene_viewer/ Li-COR Odyssey N/A https://www.licor.com/bio/products/imaging_systems/odyssey Other Catalogue of Somatic Mutations in Cancer v80; Cancer gene census tumor suppressor annotations April 2017 Forbes et al., 2017 http://cancer.sanger.ac.uk/cosmic Tumor suppressor predictions Tokheim et al., 2016 http://www.pnas.org/content/suppl/2016/11/22/1616440113.DCSupplemental/pnas.1616440113.sd04.xlsx Open in a separate window KEY RESOURCES TABLE

Article Title: Recruitment enhancement varies by taxonomic group and oyster reef habitat characteristics.
Article Snippet: All analyses were conducted in R 3.6.1 (R Development Core Team, 2019) on the RStudio IDE 1.2.1335 (RStudio Team, 2019).

Expressing:

Article Title: The effect of vortioxetine on faecal microbiota in high-fat diet-exposed mice-A link to weight protection.
Article Snippet: Funding information Axel Muusfeldt fonden; Region Midtjylland Abstract Objective: Weight gain is a common side effect of antidepressive treatment, causing distress among patients and caretakers as it can lead to treatment discontinuation and complications such as diabetes type II and cardiovascular disease.. Vortioxetine is one of the newer antidepressants and the pharmacodynamics differ from the selective serotonin reuptake inhibitors.. It is marketed as being weight neutral; however, there is little evidence as to why.

Article Title: Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures
Article Snippet: R Studio Desktop IDE 1.0.143 , RStudio , https://rstudio.com/.

Article Title: Quality of life in idiopathic pulmonary fibrosis in Latin American countries.
Article Snippet: Data were collected in Excel and analyzed with Stata 13.1 (Stata Corp LP, College Station, TX, USA) and R Studio IDE 1.4.1103.

Article Title: Distinct Immunological Landscapes Characterize Inherited and Sporadic Mismatch Repair Deficient Endometrial Cancer
Article Snippet: Statistical analyses were conducted in R 3.4.0 × 64/RStudio IDE 1.0.143.

Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Desktop IDE 1.0.143 RStudio https://www.rstudio.com Bioconductor R packages 3.5 Bioconductor http://bioconductor.org Tidyverse R packages 1.1.1 CRAN https://cran.r-project.org/web/packages/tidyverse/index.html ImageJ National Institutes of Health https://imagej.nih.gov/ij/ SnapGene Viewer SnapGene www.snapgene.com/products/snapgene_viewer/ Li-COR Odyssey N/A https://www.licor.com/bio/products/imaging_systems/odyssey Other Catalogue of Somatic Mutations in Cancer v80; Cancer gene census tumor suppressor annotations April 2017 Forbes et al., 2017 http://cancer.sanger.ac.uk/cosmic Tumor suppressor predictions Tokheim et al., 2016 http://www.pnas.org/content/suppl/2016/11/22/1616440113.DCSupplemental/pnas.1616440113.sd04.xlsx Open in a separate window KEY RESOURCES TABLE

Article Title: Recruitment enhancement varies by taxonomic group and oyster reef habitat characteristics.
Article Snippet: All analyses were conducted in R 3.6.1 (R Development Core Team, 2019) on the RStudio IDE 1.2.1335 (RStudio Team, 2019).

Software:

Article Title: The effect of vortioxetine on faecal microbiota in high-fat diet-exposed mice-A link to weight protection.
Article Snippet: Funding information Axel Muusfeldt fonden; Region Midtjylland Abstract Objective: Weight gain is a common side effect of antidepressive treatment, causing distress among patients and caretakers as it can lead to treatment discontinuation and complications such as diabetes type II and cardiovascular disease.. Vortioxetine is one of the newer antidepressants and the pharmacodynamics differ from the selective serotonin reuptake inhibitors.. It is marketed as being weight neutral; however, there is little evidence as to why.

Article Title: Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures
Article Snippet: R Studio Desktop IDE 1.0.143 , RStudio , https://rstudio.com/.

Article Title: Quality of life in idiopathic pulmonary fibrosis in Latin American countries.
Article Snippet: Data were collected in Excel and analyzed with Stata 13.1 (Stata Corp LP, College Station, TX, USA) and R Studio IDE 1.4.1103.

Article Title: Distinct Immunological Landscapes Characterize Inherited and Sporadic Mismatch Repair Deficient Endometrial Cancer
Article Snippet: Statistical analyses were conducted in R 3.4.0 × 64/RStudio IDE 1.0.143.

Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Desktop IDE 1.0.143 RStudio https://www.rstudio.com Bioconductor R packages 3.5 Bioconductor http://bioconductor.org Tidyverse R packages 1.1.1 CRAN https://cran.r-project.org/web/packages/tidyverse/index.html ImageJ National Institutes of Health https://imagej.nih.gov/ij/ SnapGene Viewer SnapGene www.snapgene.com/products/snapgene_viewer/ Li-COR Odyssey N/A https://www.licor.com/bio/products/imaging_systems/odyssey Other Catalogue of Somatic Mutations in Cancer v80; Cancer gene census tumor suppressor annotations April 2017 Forbes et al., 2017 http://cancer.sanger.ac.uk/cosmic Tumor suppressor predictions Tokheim et al., 2016 http://www.pnas.org/content/suppl/2016/11/22/1616440113.DCSupplemental/pnas.1616440113.sd04.xlsx Open in a separate window KEY RESOURCES TABLE

Article Title: Recruitment enhancement varies by taxonomic group and oyster reef habitat characteristics.
Article Snippet: All analyses were conducted in R 3.6.1 (R Development Core Team, 2019) on the RStudio IDE 1.2.1335 (RStudio Team, 2019).

Transfection:

Article Title: The effect of vortioxetine on faecal microbiota in high-fat diet-exposed mice-A link to weight protection.
Article Snippet: Funding information Axel Muusfeldt fonden; Region Midtjylland Abstract Objective: Weight gain is a common side effect of antidepressive treatment, causing distress among patients and caretakers as it can lead to treatment discontinuation and complications such as diabetes type II and cardiovascular disease.. Vortioxetine is one of the newer antidepressants and the pharmacodynamics differ from the selective serotonin reuptake inhibitors.. It is marketed as being weight neutral; however, there is little evidence as to why.

Article Title: Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures
Article Snippet: R Studio Desktop IDE 1.0.143 , RStudio , https://rstudio.com/.

Article Title: Quality of life in idiopathic pulmonary fibrosis in Latin American countries.
Article Snippet: Data were collected in Excel and analyzed with Stata 13.1 (Stata Corp LP, College Station, TX, USA) and R Studio IDE 1.4.1103.

Article Title: Distinct Immunological Landscapes Characterize Inherited and Sporadic Mismatch Repair Deficient Endometrial Cancer
Article Snippet: Statistical analyses were conducted in R 3.4.0 × 64/RStudio IDE 1.0.143.

Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Desktop IDE 1.0.143 RStudio https://www.rstudio.com Bioconductor R packages 3.5 Bioconductor http://bioconductor.org Tidyverse R packages 1.1.1 CRAN https://cran.r-project.org/web/packages/tidyverse/index.html ImageJ National Institutes of Health https://imagej.nih.gov/ij/ SnapGene Viewer SnapGene www.snapgene.com/products/snapgene_viewer/ Li-COR Odyssey N/A https://www.licor.com/bio/products/imaging_systems/odyssey Other Catalogue of Somatic Mutations in Cancer v80; Cancer gene census tumor suppressor annotations April 2017 Forbes et al., 2017 http://cancer.sanger.ac.uk/cosmic Tumor suppressor predictions Tokheim et al., 2016 http://www.pnas.org/content/suppl/2016/11/22/1616440113.DCSupplemental/pnas.1616440113.sd04.xlsx Open in a separate window KEY RESOURCES TABLE

Article Title: Recruitment enhancement varies by taxonomic group and oyster reef habitat characteristics.
Article Snippet: All analyses were conducted in R 3.6.1 (R Development Core Team, 2019) on the RStudio IDE 1.2.1335 (RStudio Team, 2019).

Polymerase Chain Reaction:

Article Title: The effect of vortioxetine on faecal microbiota in high-fat diet-exposed mice-A link to weight protection.
Article Snippet: Funding information Axel Muusfeldt fonden; Region Midtjylland Abstract Objective: Weight gain is a common side effect of antidepressive treatment, causing distress among patients and caretakers as it can lead to treatment discontinuation and complications such as diabetes type II and cardiovascular disease.. Vortioxetine is one of the newer antidepressants and the pharmacodynamics differ from the selective serotonin reuptake inhibitors.. It is marketed as being weight neutral; however, there is little evidence as to why.

Article Title: Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures
Article Snippet: R Studio Desktop IDE 1.0.143 , RStudio , https://rstudio.com/.

Article Title: Quality of life in idiopathic pulmonary fibrosis in Latin American countries.
Article Snippet: Data were collected in Excel and analyzed with Stata 13.1 (Stata Corp LP, College Station, TX, USA) and R Studio IDE 1.4.1103.

Article Title: Distinct Immunological Landscapes Characterize Inherited and Sporadic Mismatch Repair Deficient Endometrial Cancer
Article Snippet: Statistical analyses were conducted in R 3.4.0 × 64/RStudio IDE 1.0.143.

Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Desktop IDE 1.0.143 RStudio https://www.rstudio.com Bioconductor R packages 3.5 Bioconductor http://bioconductor.org Tidyverse R packages 1.1.1 CRAN https://cran.r-project.org/web/packages/tidyverse/index.html ImageJ National Institutes of Health https://imagej.nih.gov/ij/ SnapGene Viewer SnapGene www.snapgene.com/products/snapgene_viewer/ Li-COR Odyssey N/A https://www.licor.com/bio/products/imaging_systems/odyssey Other Catalogue of Somatic Mutations in Cancer v80; Cancer gene census tumor suppressor annotations April 2017 Forbes et al., 2017 http://cancer.sanger.ac.uk/cosmic Tumor suppressor predictions Tokheim et al., 2016 http://www.pnas.org/content/suppl/2016/11/22/1616440113.DCSupplemental/pnas.1616440113.sd04.xlsx Open in a separate window KEY RESOURCES TABLE

Article Title: Recruitment enhancement varies by taxonomic group and oyster reef habitat characteristics.
Article Snippet: All analyses were conducted in R 3.6.1 (R Development Core Team, 2019) on the RStudio IDE 1.2.1335 (RStudio Team, 2019).

Clone Assay:

Article Title: The effect of vortioxetine on faecal microbiota in high-fat diet-exposed mice-A link to weight protection.
Article Snippet: Funding information Axel Muusfeldt fonden; Region Midtjylland Abstract Objective: Weight gain is a common side effect of antidepressive treatment, causing distress among patients and caretakers as it can lead to treatment discontinuation and complications such as diabetes type II and cardiovascular disease.. Vortioxetine is one of the newer antidepressants and the pharmacodynamics differ from the selective serotonin reuptake inhibitors.. It is marketed as being weight neutral; however, there is little evidence as to why.

Article Title: Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures
Article Snippet: R Studio Desktop IDE 1.0.143 , RStudio , https://rstudio.com/.

Article Title: Quality of life in idiopathic pulmonary fibrosis in Latin American countries.
Article Snippet: Data were collected in Excel and analyzed with Stata 13.1 (Stata Corp LP, College Station, TX, USA) and R Studio IDE 1.4.1103.

Article Title: Distinct Immunological Landscapes Characterize Inherited and Sporadic Mismatch Repair Deficient Endometrial Cancer
Article Snippet: Statistical analyses were conducted in R 3.4.0 × 64/RStudio IDE 1.0.143.

Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Desktop IDE 1.0.143 RStudio https://www.rstudio.com Bioconductor R packages 3.5 Bioconductor http://bioconductor.org Tidyverse R packages 1.1.1 CRAN https://cran.r-project.org/web/packages/tidyverse/index.html ImageJ National Institutes of Health https://imagej.nih.gov/ij/ SnapGene Viewer SnapGene www.snapgene.com/products/snapgene_viewer/ Li-COR Odyssey N/A https://www.licor.com/bio/products/imaging_systems/odyssey Other Catalogue of Somatic Mutations in Cancer v80; Cancer gene census tumor suppressor annotations April 2017 Forbes et al., 2017 http://cancer.sanger.ac.uk/cosmic Tumor suppressor predictions Tokheim et al., 2016 http://www.pnas.org/content/suppl/2016/11/22/1616440113.DCSupplemental/pnas.1616440113.sd04.xlsx Open in a separate window KEY RESOURCES TABLE

Article Title: Recruitment enhancement varies by taxonomic group and oyster reef habitat characteristics.
Article Snippet: All analyses were conducted in R 3.6.1 (R Development Core Team, 2019) on the RStudio IDE 1.2.1335 (RStudio Team, 2019).



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World Precision Instruments glass filament needle 1 0 od 0 75 id
Experimental validation of <t>YOD1,</t> a candidate protein enriched in Y-Exos, in regulating osteogenic differentiation and macrophage polarization. (A) qPCR analysis of the effects of different concentrations of YOD1 (25 ng/mL and 50 ng/mL) on BMM polarization-related genes under non-inflammatory conditions, including TNF-α, IFN-γ, CD86, IL-4, Arg1, and CD206, n = 4. (B) Corresponding effects under LPS-induced inflammatory conditions, n = 4. (C) Western blotting analysis of YOD1, VCL, and <t>IDE</t> protein expression levels in plasma exosomes from donors of different ages, with TSG101 as the loading control, n = 3. (D-E) Flow cytometric analysis and quantification of CD86⁺ and CD206⁺ BMMs after YOD1 treatment under non-LPS and LPS conditions, n=3. (F) Western blotting analysis of YOD1 expression in BMMs and BMSCs after Y-Exos treatment, with GAPDH as the loading control, n = 3. (G) ALP staining and ARS staining evaluating the effect of YOD1 on BMSC osteogenic differentiation (scale bar = 100 μm), n = 3. (H) TRAP staining and F-actin ring staining evaluating the effect of YOD1 on BMM osteoclast differentiation (scale bar = 100 μm), n = 4. (I-J) Quantification of osteogenic differentiation (I: ALP activity and ARS staining) and osteoclast differentiation (J: osteoclast nuclei number and osteoclast area). Data are presented as mean ± SD. *P < 0.05; NS, no significant difference.
Glass Filament Needle 1 0 Od 0 75 Id, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Experimental validation of YOD1, a candidate protein enriched in Y-Exos, in regulating osteogenic differentiation and macrophage polarization. (A) qPCR analysis of the effects of different concentrations of YOD1 (25 ng/mL and 50 ng/mL) on BMM polarization-related genes under non-inflammatory conditions, including TNF-α, IFN-γ, CD86, IL-4, Arg1, and CD206, n = 4. (B) Corresponding effects under LPS-induced inflammatory conditions, n = 4. (C) Western blotting analysis of YOD1, VCL, and IDE protein expression levels in plasma exosomes from donors of different ages, with TSG101 as the loading control, n = 3. (D-E) Flow cytometric analysis and quantification of CD86⁺ and CD206⁺ BMMs after YOD1 treatment under non-LPS and LPS conditions, n=3. (F) Western blotting analysis of YOD1 expression in BMMs and BMSCs after Y-Exos treatment, with GAPDH as the loading control, n = 3. (G) ALP staining and ARS staining evaluating the effect of YOD1 on BMSC osteogenic differentiation (scale bar = 100 μm), n = 3. (H) TRAP staining and F-actin ring staining evaluating the effect of YOD1 on BMM osteoclast differentiation (scale bar = 100 μm), n = 4. (I-J) Quantification of osteogenic differentiation (I: ALP activity and ARS staining) and osteoclast differentiation (J: osteoclast nuclei number and osteoclast area). Data are presented as mean ± SD. *P < 0.05; NS, no significant difference.

Journal: International Journal of Biological Sciences

Article Title: Young plasma-derived exosomes deliver YOD1 to induce M2 polarization and ameliorate osteoporosis via mTOR signaling pathways

doi: 10.7150/ijbs.138620

Figure Lengend Snippet: Experimental validation of YOD1, a candidate protein enriched in Y-Exos, in regulating osteogenic differentiation and macrophage polarization. (A) qPCR analysis of the effects of different concentrations of YOD1 (25 ng/mL and 50 ng/mL) on BMM polarization-related genes under non-inflammatory conditions, including TNF-α, IFN-γ, CD86, IL-4, Arg1, and CD206, n = 4. (B) Corresponding effects under LPS-induced inflammatory conditions, n = 4. (C) Western blotting analysis of YOD1, VCL, and IDE protein expression levels in plasma exosomes from donors of different ages, with TSG101 as the loading control, n = 3. (D-E) Flow cytometric analysis and quantification of CD86⁺ and CD206⁺ BMMs after YOD1 treatment under non-LPS and LPS conditions, n=3. (F) Western blotting analysis of YOD1 expression in BMMs and BMSCs after Y-Exos treatment, with GAPDH as the loading control, n = 3. (G) ALP staining and ARS staining evaluating the effect of YOD1 on BMSC osteogenic differentiation (scale bar = 100 μm), n = 3. (H) TRAP staining and F-actin ring staining evaluating the effect of YOD1 on BMM osteoclast differentiation (scale bar = 100 μm), n = 4. (I-J) Quantification of osteogenic differentiation (I: ALP activity and ARS staining) and osteoclast differentiation (J: osteoclast nuclei number and osteoclast area). Data are presented as mean ± SD. *P < 0.05; NS, no significant difference.

Article Snippet: The primary and secondary antibodies are listed as follows: TSG101 Rabbit antibody (Proteintech, China, 28283-1-AP), CD63 Rabbit antibody (Proteintech, China, 25682-1-AP), CD9 Rabbit antibody (Proteintech, China, 20597-1-AP), Calnexin Rabbit antibody (Proteintech, China, 10427-2-AP), CD86 Rabbit antibody(Immunoway, USA, YM8023), CD206 Rabbit antibody (Immunoway, USA, YM8349), iNOS Rabbit antibody(Proteintech, China, 22226-1-AP), ARG1 Rabbit antibody (Abcam, China, ab96183), RUNX2 Rabbit antibody (Proteintech, China, 20700-1-AP), Osteocalcin Rabbit antibody(Servicebio, China, GB11233-100), ACTIN Mouse antibody(Proteintech, China, 66009-1-Ig), YOD1 Rabbit antibody (Proteintech, China, 25370-1-AP), IDE Rabbit antibody (Proteintech, China, 21728-1-AP), VCL Rabbit antibody (Proteintech, China, 26520-1-AP), GAPDH Rabbit antibody (BOSTER, China, A00227-1), ITCH Rabbit antibody (Proteintech, China, 20920-1-AP), LATS1 Rabbit antibody (Proteintech, China, 17049-1-AP), Phospho-YAP1 (Ser127) Rabbit antibody (Proteintech, China, 80694-2-RR), YAP1 Rabbit antibody (Proteintech, China, 13584-1-AP), Phospho-AKT Rabbit antibody (Proteintech, China, 29163-1-AP), AKT Rabbit antibody (Proteintech, China, 10176-2-AP), Phospho-mTOR Rabbit antibody(ZenBio, China, 381557), mTOR Rabbit antibody (HUA BIO, China, ET1608-5), NFATC1 Mouse antibody (Proteintech, China, 66963-1-Ig).

Techniques: Biomarker Discovery, Analysis, Western Blot, Expressing, Clinical Proteomics, Exosomes, Control, Staining, Activity Assay