id7000 software v2 1 (Sony Biotechnology)
Sony Biotechnology is a verified supplier 
Sony Biotechnology manufactures this product 
99
Structured Review
Sony Biotechnology
id7000 software v2 1
Id7000 Software V2 1, supplied by Sony Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 781 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/id7000+software/ID7000+Spectral+Cell+Analyzer/pmc12936743-159-0-4
Average 99 stars, based on 781 article reviews
Id7000 Software V2 1, supplied by Sony Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 781 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/id7000+software/ID7000+Spectral+Cell+Analyzer/pmc12936743-159-0-4
Average 99 stars, based on 781 article reviews
id7000 software v2 1 - by Bioz Stars,
2026-10
99/100 stars
Images
1) Product Images from "Protocol for generating high-quality, 45-color spectral flow cytometry data for unsupervised clustering to investigate aging in human PBMCs"
Article Title: Protocol for generating high-quality, 45-color spectral flow cytometry data for unsupervised clustering to investigate aging in human PBMCs
Journal: STAR Protocols
doi: 10.1016/j.xpro.2026.104380
Figure Legend Snippet: Optimization of a 45-color spectral panel (A) “Spectral Viewer” overview of all 45 fluorescence spectra acquired using a six-laser–configured ID7000 (320, 355, 405, 488, 561, and 637 nm) with 182 detector channels. (B) Spillover spreading matrix calculated in FlowJo V10.10.0 from single stains. (C) Titration of antibodies in human PBMCs. The viability dye Zombie NIR and CD73-biotin/Qdot 525 streptavidin were independently optimized (see for final volumes).
Techniques Used: Fluorescence, Titration
Figure Legend Snippet: Autofluorescence (AF) unmixing workflow for the 45-color panel (A) Ribbon plot showing the fluorescence spectrum of unstained PBMCs. Autofluorescence is especially excited by the 320 nm and 355 nm lasers. (B) In the ID7000 Software, the “Autofluorescence Finder” tool is employed to identify and unmix the AF of two distinct populations within the AF root gate, excited by the 320 nm and 355 nm lasers. (C) AF-A and AF-B are highlighted in the “Spectral Reference Viewer” among the 45-color + 2 AF spectra. The fluorochromes with the greatest AF overlap are BUV496 (turquoise) and SparkUV 387 (purple). (D) AF unmixing improves population separation by extracting AF contributions to the negative population for CD28-BUV496 and CD14-SparkUV 387.
Techniques Used: Fluorescence, Software
Related Articles
Staining:Article Title: Targeting CD28 on T lineage malignancies with chimeric antigen receptor T cells. Article Snippet: Leukemia-cell proliferation was monitored by Incucyte SX5 live-cell imaging in technical triplicates For co-culture assay of PBMCswith autologous CAR T cells, 1 × 106 CTV-labeled CART cells or untransduced T cellswere co-cultured at 1:1 effector to target (E:T) ratiowith correspondingPBMCs ina 6-well dish. .. After 24 and 48 h, cells were harvested and stained with a 25-color spectral panel (Supplementary Table 4) before all events were recorded at the Article Title: Microbiota-regulated TCDCA associates with Parkinson's disease severity and promotes BBB dysfunction through VSMC TGR5 signaling in mice. Article Snippet: .. Stained cells were analyzed using a Single Cell:Article Title: PRMT7 restricts CD8+ T cells expansion via the NF-ΚB pathway. Article Snippet: For tumor-infiltrating lymphocytes (TIL) analysis, primary tumors were collected at end point, weighed, mechanically diced, and digested in RPMI + 1mg/ml in the presence of collagenase P (2mg/ml, Sigma-Aldrich) and DNase I (50μg/ml, Sigma-Aldrich) for 30min at 37 °C. .. Next, cells were pipetted into a single-cell suspension, blocked with anti-mouse CD16/CD32 antibody (Fc block; BD Biosciences) for 15min and stainedwith fluorescence-conjugated antibodies for 30min and then acquired either with a BD LSR Fortessa HTS or with a Suspension:Article Title: PRMT7 restricts CD8+ T cells expansion via the NF-ΚB pathway. Article Snippet: For tumor-infiltrating lymphocytes (TIL) analysis, primary tumors were collected at end point, weighed, mechanically diced, and digested in RPMI + 1mg/ml in the presence of collagenase P (2mg/ml, Sigma-Aldrich) and DNase I (50μg/ml, Sigma-Aldrich) for 30min at 37 °C. .. Next, cells were pipetted into a single-cell suspension, blocked with anti-mouse CD16/CD32 antibody (Fc block; BD Biosciences) for 15min and stainedwith fluorescence-conjugated antibodies for 30min and then acquired either with a BD LSR Fortessa HTS or with a Blocking Assay:Article Title: PRMT7 restricts CD8+ T cells expansion via the NF-ΚB pathway. Article Snippet: For tumor-infiltrating lymphocytes (TIL) analysis, primary tumors were collected at end point, weighed, mechanically diced, and digested in RPMI + 1mg/ml in the presence of collagenase P (2mg/ml, Sigma-Aldrich) and DNase I (50μg/ml, Sigma-Aldrich) for 30min at 37 °C. .. Next, cells were pipetted into a single-cell suspension, blocked with anti-mouse CD16/CD32 antibody (Fc block; BD Biosciences) for 15min and stainedwith fluorescence-conjugated antibodies for 30min and then acquired either with a BD LSR Fortessa HTS or with a Fluorescence:Article Title: PRMT7 restricts CD8+ T cells expansion via the NF-ΚB pathway. Article Snippet: For tumor-infiltrating lymphocytes (TIL) analysis, primary tumors were collected at end point, weighed, mechanically diced, and digested in RPMI + 1mg/ml in the presence of collagenase P (2mg/ml, Sigma-Aldrich) and DNase I (50μg/ml, Sigma-Aldrich) for 30min at 37 °C. .. Next, cells were pipetted into a single-cell suspension, blocked with anti-mouse CD16/CD32 antibody (Fc block; BD Biosciences) for 15min and stainedwith fluorescence-conjugated antibodies for 30min and then acquired either with a BD LSR Fortessa HTS or with a Article Title: Δ133p53α, a senescence-inhibitory p53 isoform, attenuates accelerated aging in progeria mice Article Snippet: T lymphocytes were isolated by negative selection using the EasySep Mouse T Cell Isolation Kit (STEMCELL Technologies, #19851). .. Flow cytometry was performed according to standard procedures to detect CD3 expression using an anti-CD3-BUV395 antibody (BD Biosciences, #563565) and EGFP fluorescence in the FITC/EGFP channel, followed by data acquisition using a Sony Flow Cytometry:Article Title: PRMT7 restricts CD8+ T cells expansion via the NF-ΚB pathway. Article Snippet: For tumor-infiltrating lymphocytes (TIL) analysis, primary tumors were collected at end point, weighed, mechanically diced, and digested in RPMI + 1mg/ml in the presence of collagenase P (2mg/ml, Sigma-Aldrich) and DNase I (50μg/ml, Sigma-Aldrich) for 30min at 37 °C. .. Next, cells were pipetted into a single-cell suspension, blocked with anti-mouse CD16/CD32 antibody (Fc block; BD Biosciences) for 15min and stainedwith fluorescence-conjugated antibodies for 30min and then acquired either with a BD LSR Fortessa HTS or with a Article Title: A preinvasive regulatory T cell axis for lung cancer interception. Article Snippet: BD FACS Aria Fusion and BD Aria III for cell sorting (BD Bioscience). .. Article Title: Δ133p53α, a senescence-inhibitory p53 isoform, attenuates accelerated aging in progeria mice Article Snippet: T lymphocytes were isolated by negative selection using the EasySep Mouse T Cell Isolation Kit (STEMCELL Technologies, #19851). .. Flow cytometry was performed according to standard procedures to detect CD3 expression using an anti-CD3-BUV395 antibody (BD Biosciences, #563565) and EGFP fluorescence in the FITC/EGFP channel, followed by data acquisition using a Sony Article Title: CRISPR–Cas9 Editing of Myeloid Lectins Reveals Target-Dependent Transcriptional Remodeling in Tumor-Associated Macrophages Article Snippet: Dead cells were stained with Ghost Dye Violet 510 Fixable Viability Dye (1:1000; Cell Signaling Technology, #59863) for 15 min at 4°C. .. Cells were resuspended in FACS buffer (containing Ca2+ and Mg2+, 2% FBS) and acquired using a Sony Article Title: Compartment-specific PPARδ activity controls the outcome of cancer-immune cell competition and immunotherapy efficacy. Article Snippet: Absolute numbers were calculated based on acquired cell events and the ratio of acquired to total CountBright Absolute Counting Beads (Invitrogen, C36950) added to the sample. .. Instrument Flow cytometry data was acquired on a Becton Dickinson LSRII Cell Analyzer or Sony Article Title: Microbiota-regulated TCDCA associates with Parkinson's disease severity and promotes BBB dysfunction through VSMC TGR5 signaling in mice. Article Snippet: .. Stained cells were analyzed using a Software:Article Title: PRMT7 restricts CD8+ T cells expansion via the NF-ΚB pathway. Article Snippet: For tumor-infiltrating lymphocytes (TIL) analysis, primary tumors were collected at end point, weighed, mechanically diced, and digested in RPMI + 1mg/ml in the presence of collagenase P (2mg/ml, Sigma-Aldrich) and DNase I (50μg/ml, Sigma-Aldrich) for 30min at 37 °C. .. Next, cells were pipetted into a single-cell suspension, blocked with anti-mouse CD16/CD32 antibody (Fc block; BD Biosciences) for 15min and stainedwith fluorescence-conjugated antibodies for 30min and then acquired either with a BD LSR Fortessa HTS or with a Article Title: A preinvasive regulatory T cell axis for lung cancer interception. Article Snippet: BD FACS Aria Fusion and BD Aria III for cell sorting (BD Bioscience). .. Article Title: Δ133p53α, a senescence-inhibitory p53 isoform, attenuates accelerated aging in progeria mice Article Snippet: T lymphocytes were isolated by negative selection using the EasySep Mouse T Cell Isolation Kit (STEMCELL Technologies, #19851). .. Flow cytometry was performed according to standard procedures to detect CD3 expression using an anti-CD3-BUV395 antibody (BD Biosciences, #563565) and EGFP fluorescence in the FITC/EGFP channel, followed by data acquisition using a Sony Cytometry:Article Title: Δ133p53α, a senescence-inhibitory p53 isoform, attenuates accelerated aging in progeria mice Article Snippet: T lymphocytes were isolated by negative selection using the EasySep Mouse T Cell Isolation Kit (STEMCELL Technologies, #19851). .. Flow cytometry was performed according to standard procedures to detect CD3 expression using an anti-CD3-BUV395 antibody (BD Biosciences, #563565) and EGFP fluorescence in the FITC/EGFP channel, followed by data acquisition using a Sony Article Title: Compartment-specific PPARδ activity controls the outcome of cancer-immune cell competition and immunotherapy efficacy. Article Snippet: Absolute numbers were calculated based on acquired cell events and the ratio of acquired to total CountBright Absolute Counting Beads (Invitrogen, C36950) added to the sample. .. Instrument Flow cytometry data was acquired on a Becton Dickinson LSRII Cell Analyzer or Sony Expressing:Article Title: Δ133p53α, a senescence-inhibitory p53 isoform, attenuates accelerated aging in progeria mice Article Snippet: T lymphocytes were isolated by negative selection using the EasySep Mouse T Cell Isolation Kit (STEMCELL Technologies, #19851). .. Flow cytometry was performed according to standard procedures to detect CD3 expression using an anti-CD3-BUV395 antibody (BD Biosciences, #563565) and EGFP fluorescence in the FITC/EGFP channel, followed by data acquisition using a Sony Analysis:Article Title: Δ133p53α, a senescence-inhibitory p53 isoform, attenuates accelerated aging in progeria mice Article Snippet: T lymphocytes were isolated by negative selection using the EasySep Mouse T Cell Isolation Kit (STEMCELL Technologies, #19851). .. Flow cytometry was performed according to standard procedures to detect CD3 expression using an anti-CD3-BUV395 antibody (BD Biosciences, #563565) and EGFP fluorescence in the FITC/EGFP channel, followed by data acquisition using a Sony FACS:Article Title: CRISPR–Cas9 Editing of Myeloid Lectins Reveals Target-Dependent Transcriptional Remodeling in Tumor-Associated Macrophages Article Snippet: Dead cells were stained with Ghost Dye Violet 510 Fixable Viability Dye (1:1000; Cell Signaling Technology, #59863) for 15 min at 4°C. .. Cells were resuspended in FACS buffer (containing Ca2+ and Mg2+, 2% FBS) and acquired using a Sony |