hypertonic buffer (Active Motif)

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Active Motif hypertonic buffer
Hypertonic Buffer, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hypertonic+buffer+b/hypotonic+buffer/pmc04573256-145-6-8
Average 90 stars, based on 1 article reviews

hypertonic buffer - by Bioz Stars, 2026-11

90/100 stars

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Lysis:

Article Title: BCAT1 Associates with DNA Repair Proteins KU70 and KU80 and Contributes to Regulate DNA Repair in T-Cell Acute Lymphoblastic Leukemia (T-ALL)
Article Snippet: .. Cytoplasmic extracts were obtained using hypotonic lysis buffer (Active Motif, Carlsbad, CA, USA) supplemented with phosphatase and protease inhibitor cocktails. .. The lysates were cleared by centrifugation at 14,000× g for 30 min. NaCl concentration in cytoplasmic extracts was adjusted to 150 mM and pre-cleared with Protein G Plus–agarose (sc-2002; Santa Cruz Biotechnology, Dallas, TX, USA) at 4° for 30 min.

Protease Inhibitor:

Article Title: Interaction between hormone-sensitive lipase and ChREBP in fat cells controls insulin sensitivity.
Article Snippet: .. For endogenous co-immunoprecipitation in hMADS adipocytes, cells were lysed for 15 min in 1× hypotonic buffer (Active Motif) with 4% 25× protease inhibitor cocktail (Roche) and 1 mM orthovanadate (Sigma). .. Cell debris and fat were discarded after 12,700 r.p.m. centrifugation at 4 °C for 15 min. Preclearing was performed at 4 °C for 30 min using 50 μ l protein G and 4 μ g control rabbit (2729, Cell Signaling Technology) or mouse (sc-2025, Santa Cruz) IgG.

other:

Article Title: Phosphorylation of Npas4 by MAPK Regulates Reward-Related Gene Expression and Behaviors.
Article Snippet: The striatum and NAc (approximately +1.54 mm from Bregma) were collected using 3mm diameter biopsy punch (Miltex, NY, USA) based on the Paxinos and Franklin ‘‘the mouse brain in stereotaxic coordinates.’’ Collected tissues were homogenized with Hypotonic buffer (Nuclear Extract Kit, Active Motif, CA, USA) containing protease inhibitor cocktail and PhosStop using potter homogenizer and incubated on ice for 15 min.

Article Title: A constitutional isomer selective chemical proteomic strategy for system-wide profiling of protein lysine 5-hydroxylation
Article Snippet: Cells were briefly rinsed and scraped down with PBS and were lysed in the hypotonic buffer (Active motif, Carlsbad, CA) with 0.5% NP40 and 1 mM PMSF (ThermoFisher Scientific, Waltham, MA) and incubated on ice with manual rotation from time to time for 10 minutes followed by centrifugation at 16 000× g to obtain the nuclear pellets.

Article Title: Biliverdin reductase deficiency triggers an endothelial-to-mesenchymal transition in human endothelial cells.
Article Snippet: 16 Endothelial dysfunction accompanied by the loss of endothelial cell phenotype plays 17 an essential role in cardiovascular diseases.. Here, we report that inhibition of biliverdin 18 reductase (BVR), the enzyme of the heme degradation pathway converting biliverdin to 19 bilirubin, shifts endothelial phenotype of the primary human aortic endothelial cells (HAECs) 20 to mesenchymal-like one.. It is reflected by the loss of endothelial markers and angiogenic 21 response, with concomitant acquiring of mesenchymal markers, increased migratory capacity 22 and metalloproteinase activity.

Incubation:

Article Title: Hydroxylumisterols, Photoproducts of Pre-Vitamin D3, Protect Human Keratinocytes against UVB-Induced Damage
Article Snippet: .. The cell pellets were collected by centrifuging at 2000 × g for 5 min, re-suspended in 200 μL of 1X hypotonic buffer (Active motif, Carlsbad, CA, USA), and further incubated on ice for 15 min. ..

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