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Servicebio Inc situ hybridization fixative solution
Situ Hybridization Fixative Solution, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hybridization+solution/fixative+hybridization+situ/pm41897303-110-5-9
Average 86 stars, based on 1 article reviews
situ hybridization fixative solution - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

Fluorescence:

Article Title: Evolutionary conservation and divergence of phagocytic and coagulation programs across bilaterian circulating immune cells.
Article Snippet: .. 10 11 Fluorescence In situ hybridization 12 Cell slides were fixed using in situ hybridization fixative (Servicebio, #CR2207020) for 20 min 13 and washed in PBS (pH 7.4) on a decolorization shaker three times for 5 min each. .. Proteinase K 14 (Servicebio, #G1234) (20 μg/ml) was added at 37 °C for digestion.

Article Title: The Positive Feedback Loop of Hypoxia-Inducible Factor-1 α /miR-295/Factor Inhibiting Hypoxia-Inducible Factor-1 in Hyperuricemic Nephropathy
Article Snippet: .. The Digoxigenin-labeled mmu-miR-295 locked nucleic acid (LNA) probe and Fluorescence in situ hybridization (FISH) Kit were from Servicebio (Wuhan, China). miR-295 mimic, anti-miR-295 LNA, and FIH-1 siRNA were from Ruibo (Guangzhou, China). .. YC-1 was from Selleck Chemicals (Houston).

Article Title: Rhubarb attenuates intracerebral hemorrhage injury by upregulating Snord17 to inhibit PANoptosis.
Article Snippet: Background: Intracerebral hemorrhage (ICH) is the most severe subtype of stroke and currently lacks effective therapies.. PANoptosis, a novel programmed cell death mode, plays an important role in ICH.. Rhubarb has shown neuroprotective potential in ICH, but its mechanisms remain unclear.

Article Title: Spatiotemporally engineered microneedle for microenvironment remodeling propels mucosal regeneration after tracheal mucosal injury
Article Snippet: .. According to the method reported in previous study [ ], Fluorescence In Situ Hybridization (FISH) test was conducted for bacteria (Servicebio, Eub338) to assess bacterial distribution. .. IF staining was performed for iNOS (Abcam, ab283655), CD206 (Proteintech, 18704-1-AP), and CD31 (Abcam, ab28364) to assess inflammatory marker expression, and vascularization, respectively.

Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
Article Snippet: Human LUAD sections were stained with anti-CD68 (ABclonal, Cat# A23205), and murine lung tumor sections were stained with anti-F4/80 (ABclonal, Cat# A23788). .. circSMAD4 signals were detected on paraffin-embedded sections using an RNA fluorescence in situ hybridization kit (Servicebio, RNASweAMITM ISH CY3 Detection Kit, Cat# GF002-50T) with a custom-designed probe targeting the circSMAD4 back-splice junction. .. Nuclei were counterstained with DAPI (ABclonal, Cat# RM02978).

In Situ Hybridization:

Article Title: Evolutionary conservation and divergence of phagocytic and coagulation programs across bilaterian circulating immune cells.
Article Snippet: .. 10 11 Fluorescence In situ hybridization 12 Cell slides were fixed using in situ hybridization fixative (Servicebio, #CR2207020) for 20 min 13 and washed in PBS (pH 7.4) on a decolorization shaker three times for 5 min each. .. Proteinase K 14 (Servicebio, #G1234) (20 μg/ml) was added at 37 °C for digestion.

Article Title: The Positive Feedback Loop of Hypoxia-Inducible Factor-1 α /miR-295/Factor Inhibiting Hypoxia-Inducible Factor-1 in Hyperuricemic Nephropathy
Article Snippet: .. The Digoxigenin-labeled mmu-miR-295 locked nucleic acid (LNA) probe and Fluorescence in situ hybridization (FISH) Kit were from Servicebio (Wuhan, China). miR-295 mimic, anti-miR-295 LNA, and FIH-1 siRNA were from Ruibo (Guangzhou, China). .. YC-1 was from Selleck Chemicals (Houston).

Article Title: Rhubarb attenuates intracerebral hemorrhage injury by upregulating Snord17 to inhibit PANoptosis.
Article Snippet: Background: Intracerebral hemorrhage (ICH) is the most severe subtype of stroke and currently lacks effective therapies.. PANoptosis, a novel programmed cell death mode, plays an important role in ICH.. Rhubarb has shown neuroprotective potential in ICH, but its mechanisms remain unclear.

Article Title: Spatiotemporally engineered microneedle for microenvironment remodeling propels mucosal regeneration after tracheal mucosal injury
Article Snippet: .. According to the method reported in previous study [ ], Fluorescence In Situ Hybridization (FISH) test was conducted for bacteria (Servicebio, Eub338) to assess bacterial distribution. .. IF staining was performed for iNOS (Abcam, ab283655), CD206 (Proteintech, 18704-1-AP), and CD31 (Abcam, ab28364) to assess inflammatory marker expression, and vascularization, respectively.

Article Title: Comparative Transcriptomics Reveals Important Genes Underlying Heat-Tolerant Sterility in Photo-Thermo-Sensitive Male Sterile Wheat in Seed Production Environments.
Article Snippet: .. Anthers were fixed in in situ hybridization fixative solution (Servicebio, Wuhan, Hubei, China, Cat. No. G1113), dehydrated, embedded in paraffin, and sectioned into 4 μm slices [35]. ..

Article Title: HNRNPD Induces Radioresistance in Nasopharyngeal Carcinoma by Sequestering GRAMD4 mRNA in Stress Granules.
Article Snippet: .. Cells on slides were fixed in in situ hybridization fixative (Servicebio, G1113) for 20 min, followed by three washes with PBS. ..

Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
Article Snippet: Human LUAD sections were stained with anti-CD68 (ABclonal, Cat# A23205), and murine lung tumor sections were stained with anti-F4/80 (ABclonal, Cat# A23788). .. circSMAD4 signals were detected on paraffin-embedded sections using an RNA fluorescence in situ hybridization kit (Servicebio, RNASweAMITM ISH CY3 Detection Kit, Cat# GF002-50T) with a custom-designed probe targeting the circSMAD4 back-splice junction. .. Nuclei were counterstained with DAPI (ABclonal, Cat# RM02978).

Fluorescence In Situ Hybridization:

Article Title: The Positive Feedback Loop of Hypoxia-Inducible Factor-1 α /miR-295/Factor Inhibiting Hypoxia-Inducible Factor-1 in Hyperuricemic Nephropathy
Article Snippet: .. The Digoxigenin-labeled mmu-miR-295 locked nucleic acid (LNA) probe and Fluorescence in situ hybridization (FISH) Kit were from Servicebio (Wuhan, China). miR-295 mimic, anti-miR-295 LNA, and FIH-1 siRNA were from Ruibo (Guangzhou, China). .. YC-1 was from Selleck Chemicals (Houston).

Article Title: Rhubarb attenuates intracerebral hemorrhage injury by upregulating Snord17 to inhibit PANoptosis.
Article Snippet: Background: Intracerebral hemorrhage (ICH) is the most severe subtype of stroke and currently lacks effective therapies.. PANoptosis, a novel programmed cell death mode, plays an important role in ICH.. Rhubarb has shown neuroprotective potential in ICH, but its mechanisms remain unclear.

FISH:

Article Title: Spatiotemporally engineered microneedle for microenvironment remodeling propels mucosal regeneration after tracheal mucosal injury
Article Snippet: .. According to the method reported in previous study [ ], Fluorescence In Situ Hybridization (FISH) test was conducted for bacteria (Servicebio, Eub338) to assess bacterial distribution. .. IF staining was performed for iNOS (Abcam, ab283655), CD206 (Proteintech, 18704-1-AP), and CD31 (Abcam, ab28364) to assess inflammatory marker expression, and vascularization, respectively.

Bacteria:

Article Title: Spatiotemporally engineered microneedle for microenvironment remodeling propels mucosal regeneration after tracheal mucosal injury
Article Snippet: .. According to the method reported in previous study [ ], Fluorescence In Situ Hybridization (FISH) test was conducted for bacteria (Servicebio, Eub338) to assess bacterial distribution. .. IF staining was performed for iNOS (Abcam, ab283655), CD206 (Proteintech, 18704-1-AP), and CD31 (Abcam, ab28364) to assess inflammatory marker expression, and vascularization, respectively.

In Situ:

Article Title: Integrin CD11b/CD18 reprograms macrophage polarization by suppressing ERK/STAT3 signaling to enhance antitumor immunity in colitis-associated colorectal cancer.
Article Snippet: .. To evaluate in situ cellular apoptosis, terminal deoxynucleotidyl transferase dUTP nick- end labeling (TUNEL) assay was performed using a commercial fluoresceinbased detection kit (Servicebio, China, Cat# G1501) following the manufacturer’s instructions. ..

TUNEL Assay:

Article Title: Integrin CD11b/CD18 reprograms macrophage polarization by suppressing ERK/STAT3 signaling to enhance antitumor immunity in colitis-associated colorectal cancer.
Article Snippet: .. To evaluate in situ cellular apoptosis, terminal deoxynucleotidyl transferase dUTP nick- end labeling (TUNEL) assay was performed using a commercial fluoresceinbased detection kit (Servicebio, China, Cat# G1501) following the manufacturer’s instructions. ..



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