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hybridization buffer  (NimbleGen Systems GmbH)

 
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    NimbleGen Systems GmbH hybridization buffer
    Hybridization Buffer, supplied by NimbleGen Systems GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hybridization+buffer/hybridization+buffer/pmc03095972-282-20-22
    Average 90 stars, based on 1 article reviews
    hybridization buffer - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Hybridization:

    Article Title: MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury?
    Article Snippet: Following labeling, Hy3TM-labeled samples were hybridized to the miRCURYTM LNA Array (v.16.0) (Exiqon). .. According to the array manual, a total of 25 μL mixture from Hy3TM-labeled samples and 25 μL hybridization buffer was first denatured for 2 minutes at 95°C, incubated on ice for 2 minutes, and then hybridized to the microarray for 16–20 hours at 56°C in a 12-Bay Hybridization Systems (Hybridization System; Nimblegen Systems, Inc., Madison, WI, USA). .. Following hybridization, slides were washed several times using a Wash buffer kit (Exiqon), and dried by centrifugation for 5 minutes at 80 × g . Slides were then scanned using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA, USA).

    Article Title: MicroRNA-16 Modulates Melatonin-Induced Cell Growth in the Mouse-Derived Spermatogonia Cell Line GC-1 spg Cells by Targeting Ccnd1.
    Article Snippet: After stopping the labeling procedure, the Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (Exiqon) in accordance with array manual. .. The total 25 μL mixture from Hy3TM-labeled samples with 25 μL hybridization buffer were first denatured for 2 min at 95 °C, incubated on ice for 2 min, and then hybridized to the microarray for 16–20 h at 56 °C in a 12-Bay Hybridization Systems (Hybridization System–Nimblegen Systems, Inc., Madison, WI, USA). .. Following hybridization, slides were obtained and washed several times using a wash buffer kit (Exiqon) as follows.

    Article Title: The crosstalk between endothelial cells and vascular smooth muscle cells aggravates high phosphorus-induced arterial calcification
    Article Snippet: Then 3.0 μL of labeling buffer, 1.5 μL of fluorescent label (Hy3TM), 2.0 μL of DMSO, 2.0 μL of labeling enzyme were added into the mixture for incubation at 16 °C for 1 h. After stopping the labeling procedure, the Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (v.19.0) (Exiqon) according to the array manual. .. The total 25 μL mixture from Hy3TM-labeled samples with 25 μL hybridization buffer were first denatured for 2 min at 95 °C, and incubated on ice for 2 min. Then hybridized to the microarray for 16–20 h at 56 °C in a 12-Bay Hybridization Systems (Hybridization System - Nimblegen Systems, Inc., Madison, WI, USA). .. After being washed several times using Wash buffer kit (Exiqon), the slides were scanned using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA).

    Article Title: Alteration of Gene Expression, DNA Methylation, and Histone Methylation in Free Radical Scavenging Networks in Adult Mouse Hippocampus following Fetal Alcohol Exposure
    Article Snippet: .. Microarrays were hybridized at 42°C during 16 to 20h with Cy3/5 labelled DNA in NimbleGen hybridization buffer/ hybridization component A in a hybridization chamber (Hybridization System—NimbleGen Systems, Inc., Madison, WI, USA). .. Following hybridization, washing was performed using the NimbleGen Wash Buffer kit (NimbleGen Systems, Inc., Madison, WI, USA).

    Article Title: Plasma microRNA alterations between EGFR-activating mutational NSCLC patients with and without primary resistance to TKI
    Article Snippet: After stopping the labeling procedure, the Hy3-labeled samples were hybridized on the miRCURYTM LNA Array (v.18.0) (Exiqon) according to array manual. .. The total 25 μL mixture from Hy3-labeled samples with 25 μL hybridization buffer were first denatured for 2 min at 95°C, incubated on ice for 2 min. Then hybridized to the microarray for 16–20 h at 56°C in a 12-Bay Hybridization Systems (Hybridization System-Nimblegen Systems, Inc., Madison, WI, USA). .. Following hybridization, the slides were achieved, washed several times using Wash buffer kit (Exiqon).

    Article Title: Extract of Stellerachamaejasme L (ESC) inhibits growth and metastasis of human hepatocellular carcinoma via regulating microRNA expression
    Article Snippet: After stopping the labeling procedure, the Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (v.18.0) (Exiqon) according to array manual. .. The total 25 μL mixture from Hy3TM-labeled samples with 25 μL hybridization buffer were first denatured for 2 min at 95 °C, incubated on ice for 2 min. Then hybridized to the microarray for 16–20 h at 56 °C in a Hybridization Systems (Hybridization System-Nimblegen Systems, Inc., Madison, WI, USA). .. Following hybridization, the slides were achieved, washed several times using Wash buffer kit (Exiqon).

    Article Title: Tumor-associated exosomal miRNA biomarkers to differentiate metastatic vs. nonmetastatic non-small cell lung cancer.
    Article Snippet: Background: Exosomal microRNAs (miRNAs) are proposed to be excellent candidate biomarkers for clinical applications.. However, little is known about their potential value as diagnostic biomarkers for metastatic nonsmall cell lung cancer (NSCLC).. Methods: In this study, microarrays were used to determine distinct miRNA profiles of plasma exosomes in a discovery cohort of healthy donors, metastatic NSCLC and nonmetastatic NSCLC patients.

    Incubation:

    Article Title: MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury?
    Article Snippet: Following labeling, Hy3TM-labeled samples were hybridized to the miRCURYTM LNA Array (v.16.0) (Exiqon). .. According to the array manual, a total of 25 μL mixture from Hy3TM-labeled samples and 25 μL hybridization buffer was first denatured for 2 minutes at 95°C, incubated on ice for 2 minutes, and then hybridized to the microarray for 16–20 hours at 56°C in a 12-Bay Hybridization Systems (Hybridization System; Nimblegen Systems, Inc., Madison, WI, USA). .. Following hybridization, slides were washed several times using a Wash buffer kit (Exiqon), and dried by centrifugation for 5 minutes at 80 × g . Slides were then scanned using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA, USA).

    Article Title: MicroRNA-16 Modulates Melatonin-Induced Cell Growth in the Mouse-Derived Spermatogonia Cell Line GC-1 spg Cells by Targeting Ccnd1.
    Article Snippet: After stopping the labeling procedure, the Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (Exiqon) in accordance with array manual. .. The total 25 μL mixture from Hy3TM-labeled samples with 25 μL hybridization buffer were first denatured for 2 min at 95 °C, incubated on ice for 2 min, and then hybridized to the microarray for 16–20 h at 56 °C in a 12-Bay Hybridization Systems (Hybridization System–Nimblegen Systems, Inc., Madison, WI, USA). .. Following hybridization, slides were obtained and washed several times using a wash buffer kit (Exiqon) as follows.

    Article Title: The crosstalk between endothelial cells and vascular smooth muscle cells aggravates high phosphorus-induced arterial calcification
    Article Snippet: Then 3.0 μL of labeling buffer, 1.5 μL of fluorescent label (Hy3TM), 2.0 μL of DMSO, 2.0 μL of labeling enzyme were added into the mixture for incubation at 16 °C for 1 h. After stopping the labeling procedure, the Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (v.19.0) (Exiqon) according to the array manual. .. The total 25 μL mixture from Hy3TM-labeled samples with 25 μL hybridization buffer were first denatured for 2 min at 95 °C, and incubated on ice for 2 min. Then hybridized to the microarray for 16–20 h at 56 °C in a 12-Bay Hybridization Systems (Hybridization System - Nimblegen Systems, Inc., Madison, WI, USA). .. After being washed several times using Wash buffer kit (Exiqon), the slides were scanned using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA).

    Article Title: Plasma microRNA alterations between EGFR-activating mutational NSCLC patients with and without primary resistance to TKI
    Article Snippet: After stopping the labeling procedure, the Hy3-labeled samples were hybridized on the miRCURYTM LNA Array (v.18.0) (Exiqon) according to array manual. .. The total 25 μL mixture from Hy3-labeled samples with 25 μL hybridization buffer were first denatured for 2 min at 95°C, incubated on ice for 2 min. Then hybridized to the microarray for 16–20 h at 56°C in a 12-Bay Hybridization Systems (Hybridization System-Nimblegen Systems, Inc., Madison, WI, USA). .. Following hybridization, the slides were achieved, washed several times using Wash buffer kit (Exiqon).

    Article Title: Extract of Stellerachamaejasme L (ESC) inhibits growth and metastasis of human hepatocellular carcinoma via regulating microRNA expression
    Article Snippet: After stopping the labeling procedure, the Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (v.18.0) (Exiqon) according to array manual. .. The total 25 μL mixture from Hy3TM-labeled samples with 25 μL hybridization buffer were first denatured for 2 min at 95 °C, incubated on ice for 2 min. Then hybridized to the microarray for 16–20 h at 56 °C in a Hybridization Systems (Hybridization System-Nimblegen Systems, Inc., Madison, WI, USA). .. Following hybridization, the slides were achieved, washed several times using Wash buffer kit (Exiqon).

    Article Title: Tumor-associated exosomal miRNA biomarkers to differentiate metastatic vs. nonmetastatic non-small cell lung cancer.
    Article Snippet: Background: Exosomal microRNAs (miRNAs) are proposed to be excellent candidate biomarkers for clinical applications.. However, little is known about their potential value as diagnostic biomarkers for metastatic nonsmall cell lung cancer (NSCLC).. Methods: In this study, microarrays were used to determine distinct miRNA profiles of plasma exosomes in a discovery cohort of healthy donors, metastatic NSCLC and nonmetastatic NSCLC patients.

    Microarray:

    Article Title: MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury?
    Article Snippet: Following labeling, Hy3TM-labeled samples were hybridized to the miRCURYTM LNA Array (v.16.0) (Exiqon). .. According to the array manual, a total of 25 μL mixture from Hy3TM-labeled samples and 25 μL hybridization buffer was first denatured for 2 minutes at 95°C, incubated on ice for 2 minutes, and then hybridized to the microarray for 16–20 hours at 56°C in a 12-Bay Hybridization Systems (Hybridization System; Nimblegen Systems, Inc., Madison, WI, USA). .. Following hybridization, slides were washed several times using a Wash buffer kit (Exiqon), and dried by centrifugation for 5 minutes at 80 × g . Slides were then scanned using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA, USA).

    Article Title: MicroRNA-16 Modulates Melatonin-Induced Cell Growth in the Mouse-Derived Spermatogonia Cell Line GC-1 spg Cells by Targeting Ccnd1.
    Article Snippet: After stopping the labeling procedure, the Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (Exiqon) in accordance with array manual. .. The total 25 μL mixture from Hy3TM-labeled samples with 25 μL hybridization buffer were first denatured for 2 min at 95 °C, incubated on ice for 2 min, and then hybridized to the microarray for 16–20 h at 56 °C in a 12-Bay Hybridization Systems (Hybridization System–Nimblegen Systems, Inc., Madison, WI, USA). .. Following hybridization, slides were obtained and washed several times using a wash buffer kit (Exiqon) as follows.

    Article Title: The crosstalk between endothelial cells and vascular smooth muscle cells aggravates high phosphorus-induced arterial calcification
    Article Snippet: Then 3.0 μL of labeling buffer, 1.5 μL of fluorescent label (Hy3TM), 2.0 μL of DMSO, 2.0 μL of labeling enzyme were added into the mixture for incubation at 16 °C for 1 h. After stopping the labeling procedure, the Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (v.19.0) (Exiqon) according to the array manual. .. The total 25 μL mixture from Hy3TM-labeled samples with 25 μL hybridization buffer were first denatured for 2 min at 95 °C, and incubated on ice for 2 min. Then hybridized to the microarray for 16–20 h at 56 °C in a 12-Bay Hybridization Systems (Hybridization System - Nimblegen Systems, Inc., Madison, WI, USA). .. After being washed several times using Wash buffer kit (Exiqon), the slides were scanned using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA).

    Article Title: Plasma microRNA alterations between EGFR-activating mutational NSCLC patients with and without primary resistance to TKI
    Article Snippet: After stopping the labeling procedure, the Hy3-labeled samples were hybridized on the miRCURYTM LNA Array (v.18.0) (Exiqon) according to array manual. .. The total 25 μL mixture from Hy3-labeled samples with 25 μL hybridization buffer were first denatured for 2 min at 95°C, incubated on ice for 2 min. Then hybridized to the microarray for 16–20 h at 56°C in a 12-Bay Hybridization Systems (Hybridization System-Nimblegen Systems, Inc., Madison, WI, USA). .. Following hybridization, the slides were achieved, washed several times using Wash buffer kit (Exiqon).

    Article Title: Extract of Stellerachamaejasme L (ESC) inhibits growth and metastasis of human hepatocellular carcinoma via regulating microRNA expression
    Article Snippet: After stopping the labeling procedure, the Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (v.18.0) (Exiqon) according to array manual. .. The total 25 μL mixture from Hy3TM-labeled samples with 25 μL hybridization buffer were first denatured for 2 min at 95 °C, incubated on ice for 2 min. Then hybridized to the microarray for 16–20 h at 56 °C in a Hybridization Systems (Hybridization System-Nimblegen Systems, Inc., Madison, WI, USA). .. Following hybridization, the slides were achieved, washed several times using Wash buffer kit (Exiqon).

    Article Title: Tumor-associated exosomal miRNA biomarkers to differentiate metastatic vs. nonmetastatic non-small cell lung cancer.
    Article Snippet: Background: Exosomal microRNAs (miRNAs) are proposed to be excellent candidate biomarkers for clinical applications.. However, little is known about their potential value as diagnostic biomarkers for metastatic nonsmall cell lung cancer (NSCLC).. Methods: In this study, microarrays were used to determine distinct miRNA profiles of plasma exosomes in a discovery cohort of healthy donors, metastatic NSCLC and nonmetastatic NSCLC patients.

    other:

    Article Title: Identification, characterization and microRNA expression profiling of side population cells in human oral squamous cell carcinoma Tca8113 cell lines
    Article Snippet: The miRCURY Hy3/Hy5 Power labeling kit (Exiqon A/S) was used, according to the manufacturer's protocol, for miRNA labelling.



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    Image Search Results


    (A, B) The C5-hFc-siSOD1 conjugate was dosed in WT mice as a single IV or SC bolus at 1.5 mg/kg (A) or 0.14 to 9 mg/kg siRNA equivalent dose (B). Target knockdown was measured 14 days after administration, using RT-qPCR on total RNA extracts from indicated regions of the CNS. The results are expressed as a ratio to mRNA levels measured in PBS-treated animals after normalization to RpL13 and RpL30 reference genes. (A) Box plots: extends from the 25th to 75th percentiles with center line plotted at the median. Whiskers indicate the smallest and the largest experimental values (n = 8-16 animals per group from 3 independent experiments). (B) Dose-response curves in each CNS region, where experimental results are expressed as means ± SEM, and estimated ED 50 values. (C) Mice were administered with either multi-IV (Q2Dx3) at 1.5 mg/kg or single SC at 4.5 mg/kg siRNA equivalent dose of the C5-hFc-siSOD1 conjugate. Shown are in situ hybridization micrographs showing siSOD1 AS distribution (top panels, miRNAScope®) throughout the brain (sagittal sections) and spinal cord (cross-sections), and corresponding Sod1 mRNA (lower panels, RNAScope®), 14 days after the last IV or single SC (representative animal from n=4 mice per group). (D) Magnifications and neuron-specific co-staining, using a Map2 mRNA-specific probe, in indicated regions of the brain.

    Journal: bioRxiv

    Article Title: Harnessing TfR1 for Cross-Species Systemic Delivery of siRNAs to Deep Brain Regions Using Single-Domain Antibodies

    doi: 10.64898/2026.05.20.726486

    Figure Lengend Snippet: (A, B) The C5-hFc-siSOD1 conjugate was dosed in WT mice as a single IV or SC bolus at 1.5 mg/kg (A) or 0.14 to 9 mg/kg siRNA equivalent dose (B). Target knockdown was measured 14 days after administration, using RT-qPCR on total RNA extracts from indicated regions of the CNS. The results are expressed as a ratio to mRNA levels measured in PBS-treated animals after normalization to RpL13 and RpL30 reference genes. (A) Box plots: extends from the 25th to 75th percentiles with center line plotted at the median. Whiskers indicate the smallest and the largest experimental values (n = 8-16 animals per group from 3 independent experiments). (B) Dose-response curves in each CNS region, where experimental results are expressed as means ± SEM, and estimated ED 50 values. (C) Mice were administered with either multi-IV (Q2Dx3) at 1.5 mg/kg or single SC at 4.5 mg/kg siRNA equivalent dose of the C5-hFc-siSOD1 conjugate. Shown are in situ hybridization micrographs showing siSOD1 AS distribution (top panels, miRNAScope®) throughout the brain (sagittal sections) and spinal cord (cross-sections), and corresponding Sod1 mRNA (lower panels, RNAScope®), 14 days after the last IV or single SC (representative animal from n=4 mice per group). (D) Magnifications and neuron-specific co-staining, using a Map2 mRNA-specific probe, in indicated regions of the brain.

    Article Snippet: For knock-down analysis on tissue sections using in situ hybridization (RNAScope, ACD), left cerebral hemisphere half-slices were cut into one ventral and one dorsal parts and immediately immersed in 10% neutral buffer formalin (NBF) fixative solution for approximately 48h at RT.

    Techniques: Knockdown, Quantitative RT-PCR, In Situ Hybridization, RNAscope, Staining

    (A-B) In situ hybridization micrographs showing siSOD1 AS distribution (miRNAScope®) throughout the brain (sagittal sections) and spinal cord (cross-sections) (A), 14 days after multi-IV bolus (Q2D x3) of the non-binding C5neg- or the TfR1-binding B8V32-hFc-siSOD1 conjugates, at 1.5 mg/kg siRNA. (B) In situ hybridization micrographs showing siSOD1 AS distribution (top panels, miRNAScope®) and corresponding Sod1 mRNA (lower panels, RNAScope®) in retina layers of the same animals (representative animal from n=4 mice per group). Retina layers: GCL, Ganglion Cell Layer; IPL, Inner Plexiform Layer; INL, Inner Nuclear Layer; OPL, Outer Plexiform Layer; ONL, Outer Nuclear Layer; IS, Inner Segments; OS, Outer Segments; RPE, Retinal Pigment Epithelium; Chor, Choroid.

    Journal: bioRxiv

    Article Title: Harnessing TfR1 for Cross-Species Systemic Delivery of siRNAs to Deep Brain Regions Using Single-Domain Antibodies

    doi: 10.64898/2026.05.20.726486

    Figure Lengend Snippet: (A-B) In situ hybridization micrographs showing siSOD1 AS distribution (miRNAScope®) throughout the brain (sagittal sections) and spinal cord (cross-sections) (A), 14 days after multi-IV bolus (Q2D x3) of the non-binding C5neg- or the TfR1-binding B8V32-hFc-siSOD1 conjugates, at 1.5 mg/kg siRNA. (B) In situ hybridization micrographs showing siSOD1 AS distribution (top panels, miRNAScope®) and corresponding Sod1 mRNA (lower panels, RNAScope®) in retina layers of the same animals (representative animal from n=4 mice per group). Retina layers: GCL, Ganglion Cell Layer; IPL, Inner Plexiform Layer; INL, Inner Nuclear Layer; OPL, Outer Plexiform Layer; ONL, Outer Nuclear Layer; IS, Inner Segments; OS, Outer Segments; RPE, Retinal Pigment Epithelium; Chor, Choroid.

    Article Snippet: For knock-down analysis on tissue sections using in situ hybridization (RNAScope, ACD), left cerebral hemisphere half-slices were cut into one ventral and one dorsal parts and immediately immersed in 10% neutral buffer formalin (NBF) fixative solution for approximately 48h at RT.

    Techniques: In Situ Hybridization, Binding Assay, RNAscope

    Cynomolgus monkeys were dosed by 30-min IV infusion three times at 7 days intervals with the B8V32-hFc-siHPRT conjugate, at the same dose previously evaluated in hTfR-KI mice of 1.5 mg/kg siRNA equivalent dose. Plasma samples were collected at indicated timepoints for PK analysis (A). Tissues were recovered at 14 days after last injection to assess target knockdown using RT-qPCR (B) and in situ hybridization in the brain using RNAScope® on one coronal section through the putamen (top panels: approximately -1 to -3 mm from the anterior commissure, based on Calabrese et al. 2015) and one through the hippocampus (lower panels: approximately -11 to -13 mm from the anterior commissure; star indicates an artefact) (C). (D) Neuroinflammation was assessed on brain tissue samples by measurement of mRNA levels of IbA1, GFAP and C3 as markers of microglial reactivity, astrocyte reactivity and complement activation, respectively.

    Journal: bioRxiv

    Article Title: Harnessing TfR1 for Cross-Species Systemic Delivery of siRNAs to Deep Brain Regions Using Single-Domain Antibodies

    doi: 10.64898/2026.05.20.726486

    Figure Lengend Snippet: Cynomolgus monkeys were dosed by 30-min IV infusion three times at 7 days intervals with the B8V32-hFc-siHPRT conjugate, at the same dose previously evaluated in hTfR-KI mice of 1.5 mg/kg siRNA equivalent dose. Plasma samples were collected at indicated timepoints for PK analysis (A). Tissues were recovered at 14 days after last injection to assess target knockdown using RT-qPCR (B) and in situ hybridization in the brain using RNAScope® on one coronal section through the putamen (top panels: approximately -1 to -3 mm from the anterior commissure, based on Calabrese et al. 2015) and one through the hippocampus (lower panels: approximately -11 to -13 mm from the anterior commissure; star indicates an artefact) (C). (D) Neuroinflammation was assessed on brain tissue samples by measurement of mRNA levels of IbA1, GFAP and C3 as markers of microglial reactivity, astrocyte reactivity and complement activation, respectively.

    Article Snippet: For knock-down analysis on tissue sections using in situ hybridization (RNAScope, ACD), left cerebral hemisphere half-slices were cut into one ventral and one dorsal parts and immediately immersed in 10% neutral buffer formalin (NBF) fixative solution for approximately 48h at RT.

    Techniques: Clinical Proteomics, Injection, Knockdown, Quantitative RT-PCR, In Situ Hybridization, RNAscope, Activation Assay