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hcrtm probe hybridization buffer  (Molecular Instruments)

 
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    Molecular Instruments hcrtm probe hybridization buffer
    Hcrtm Probe Hybridization Buffer, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hybridization+buffer/buffer+probe+wash/pm42269093-375-31-35
    Average 86 stars, based on 1 article reviews
    hcrtm probe hybridization buffer - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Synthesized:

    Article Title: The origin and evolution of dorsal determination mechanisms in vertebrate paired appendages.
    Article Snippet: Finally, the embryos were washed to remove the excess of hairpins and stained with DAPI to be visualized in a confocal microscope Leica Stellaris 5. .. Buffers and hairpins were ordered from Molecular Instruments, Inc. DNA probes targeting lmx1bb, wnt7aa, en1a, lbx1b, and pax3a were designed according to Elagoz et al.,53 synthesized as oPools (IDT), and used in combination with hairpin amplifiers from Molecular Instruments. ..

    Suspension:

    Article Title: Protocol for purifying and sequencing cell subpopulations based on RNA FISH signal in planaria
    Article Snippet: SMART-Seq mRNA kit , Takara Biosciences , Cat# 634772. .. HCR v3 Buffers for Cells in Suspension , Molecular Instruments , N/A. .. HCR v3 probes (e.g., mmp - 1 with B1 amplifier) , Molecular Instruments , N/A.

    other:

    Article Title: Neuronal microexons modulate arousal via the cAMP-PKA-CREB pathway in zebrafish.
    Article Snippet: HCR probes for elavl3 were purchased from Molecular Instruments together with B1- Alexa647 and B2- Alexa546 amplifiers and required buffers.

    Article Title: A role for nucleolin in functional improvement in stroke
    Article Snippet: HCR 3.0 Gad1 , Molecular Instruments , N/A.

    Article Title: A role for nucleolin in functional improvement in stroke
    Article Snippet: HCR 3.0 Ncl , Molecular Instruments , N/A.

    Staining:

    Article Title: Osr1-expressing mesoderm contributes to lymphatic vessel assembly and complexity in the mammalian kidney.
    Article Snippet: .. HCR was performed as previously described.121 DNA probe sets targeting Osr1, Pax3, and Foxd1 transcripts, along with staining reagents and hairpin amplifiers, were supplied by Molecular Instruments. .. Embryos were stained with DAPI and mounted in CitiFluor AF1 on custom chamber slides.



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    Image Search Results


    (A, B) The C5-hFc-siSOD1 conjugate was dosed in WT mice as a single IV or SC bolus at 1.5 mg/kg (A) or 0.14 to 9 mg/kg siRNA equivalent dose (B). Target knockdown was measured 14 days after administration, using RT-qPCR on total RNA extracts from indicated regions of the CNS. The results are expressed as a ratio to mRNA levels measured in PBS-treated animals after normalization to RpL13 and RpL30 reference genes. (A) Box plots: extends from the 25th to 75th percentiles with center line plotted at the median. Whiskers indicate the smallest and the largest experimental values (n = 8-16 animals per group from 3 independent experiments). (B) Dose-response curves in each CNS region, where experimental results are expressed as means ± SEM, and estimated ED 50 values. (C) Mice were administered with either multi-IV (Q2Dx3) at 1.5 mg/kg or single SC at 4.5 mg/kg siRNA equivalent dose of the C5-hFc-siSOD1 conjugate. Shown are in situ hybridization micrographs showing siSOD1 AS distribution (top panels, miRNAScope®) throughout the brain (sagittal sections) and spinal cord (cross-sections), and corresponding Sod1 mRNA (lower panels, RNAScope®), 14 days after the last IV or single SC (representative animal from n=4 mice per group). (D) Magnifications and neuron-specific co-staining, using a Map2 mRNA-specific probe, in indicated regions of the brain.

    Journal: bioRxiv

    Article Title: Harnessing TfR1 for Cross-Species Systemic Delivery of siRNAs to Deep Brain Regions Using Single-Domain Antibodies

    doi: 10.64898/2026.05.20.726486

    Figure Lengend Snippet: (A, B) The C5-hFc-siSOD1 conjugate was dosed in WT mice as a single IV or SC bolus at 1.5 mg/kg (A) or 0.14 to 9 mg/kg siRNA equivalent dose (B). Target knockdown was measured 14 days after administration, using RT-qPCR on total RNA extracts from indicated regions of the CNS. The results are expressed as a ratio to mRNA levels measured in PBS-treated animals after normalization to RpL13 and RpL30 reference genes. (A) Box plots: extends from the 25th to 75th percentiles with center line plotted at the median. Whiskers indicate the smallest and the largest experimental values (n = 8-16 animals per group from 3 independent experiments). (B) Dose-response curves in each CNS region, where experimental results are expressed as means ± SEM, and estimated ED 50 values. (C) Mice were administered with either multi-IV (Q2Dx3) at 1.5 mg/kg or single SC at 4.5 mg/kg siRNA equivalent dose of the C5-hFc-siSOD1 conjugate. Shown are in situ hybridization micrographs showing siSOD1 AS distribution (top panels, miRNAScope®) throughout the brain (sagittal sections) and spinal cord (cross-sections), and corresponding Sod1 mRNA (lower panels, RNAScope®), 14 days after the last IV or single SC (representative animal from n=4 mice per group). (D) Magnifications and neuron-specific co-staining, using a Map2 mRNA-specific probe, in indicated regions of the brain.

    Article Snippet: For knock-down analysis on tissue sections using in situ hybridization (RNAScope, ACD), left cerebral hemisphere half-slices were cut into one ventral and one dorsal parts and immediately immersed in 10% neutral buffer formalin (NBF) fixative solution for approximately 48h at RT.

    Techniques: Knockdown, Quantitative RT-PCR, In Situ Hybridization, RNAscope, Staining

    (A-B) In situ hybridization micrographs showing siSOD1 AS distribution (miRNAScope®) throughout the brain (sagittal sections) and spinal cord (cross-sections) (A), 14 days after multi-IV bolus (Q2D x3) of the non-binding C5neg- or the TfR1-binding B8V32-hFc-siSOD1 conjugates, at 1.5 mg/kg siRNA. (B) In situ hybridization micrographs showing siSOD1 AS distribution (top panels, miRNAScope®) and corresponding Sod1 mRNA (lower panels, RNAScope®) in retina layers of the same animals (representative animal from n=4 mice per group). Retina layers: GCL, Ganglion Cell Layer; IPL, Inner Plexiform Layer; INL, Inner Nuclear Layer; OPL, Outer Plexiform Layer; ONL, Outer Nuclear Layer; IS, Inner Segments; OS, Outer Segments; RPE, Retinal Pigment Epithelium; Chor, Choroid.

    Journal: bioRxiv

    Article Title: Harnessing TfR1 for Cross-Species Systemic Delivery of siRNAs to Deep Brain Regions Using Single-Domain Antibodies

    doi: 10.64898/2026.05.20.726486

    Figure Lengend Snippet: (A-B) In situ hybridization micrographs showing siSOD1 AS distribution (miRNAScope®) throughout the brain (sagittal sections) and spinal cord (cross-sections) (A), 14 days after multi-IV bolus (Q2D x3) of the non-binding C5neg- or the TfR1-binding B8V32-hFc-siSOD1 conjugates, at 1.5 mg/kg siRNA. (B) In situ hybridization micrographs showing siSOD1 AS distribution (top panels, miRNAScope®) and corresponding Sod1 mRNA (lower panels, RNAScope®) in retina layers of the same animals (representative animal from n=4 mice per group). Retina layers: GCL, Ganglion Cell Layer; IPL, Inner Plexiform Layer; INL, Inner Nuclear Layer; OPL, Outer Plexiform Layer; ONL, Outer Nuclear Layer; IS, Inner Segments; OS, Outer Segments; RPE, Retinal Pigment Epithelium; Chor, Choroid.

    Article Snippet: For knock-down analysis on tissue sections using in situ hybridization (RNAScope, ACD), left cerebral hemisphere half-slices were cut into one ventral and one dorsal parts and immediately immersed in 10% neutral buffer formalin (NBF) fixative solution for approximately 48h at RT.

    Techniques: In Situ Hybridization, Binding Assay, RNAscope

    Cynomolgus monkeys were dosed by 30-min IV infusion three times at 7 days intervals with the B8V32-hFc-siHPRT conjugate, at the same dose previously evaluated in hTfR-KI mice of 1.5 mg/kg siRNA equivalent dose. Plasma samples were collected at indicated timepoints for PK analysis (A). Tissues were recovered at 14 days after last injection to assess target knockdown using RT-qPCR (B) and in situ hybridization in the brain using RNAScope® on one coronal section through the putamen (top panels: approximately -1 to -3 mm from the anterior commissure, based on Calabrese et al. 2015) and one through the hippocampus (lower panels: approximately -11 to -13 mm from the anterior commissure; star indicates an artefact) (C). (D) Neuroinflammation was assessed on brain tissue samples by measurement of mRNA levels of IbA1, GFAP and C3 as markers of microglial reactivity, astrocyte reactivity and complement activation, respectively.

    Journal: bioRxiv

    Article Title: Harnessing TfR1 for Cross-Species Systemic Delivery of siRNAs to Deep Brain Regions Using Single-Domain Antibodies

    doi: 10.64898/2026.05.20.726486

    Figure Lengend Snippet: Cynomolgus monkeys were dosed by 30-min IV infusion three times at 7 days intervals with the B8V32-hFc-siHPRT conjugate, at the same dose previously evaluated in hTfR-KI mice of 1.5 mg/kg siRNA equivalent dose. Plasma samples were collected at indicated timepoints for PK analysis (A). Tissues were recovered at 14 days after last injection to assess target knockdown using RT-qPCR (B) and in situ hybridization in the brain using RNAScope® on one coronal section through the putamen (top panels: approximately -1 to -3 mm from the anterior commissure, based on Calabrese et al. 2015) and one through the hippocampus (lower panels: approximately -11 to -13 mm from the anterior commissure; star indicates an artefact) (C). (D) Neuroinflammation was assessed on brain tissue samples by measurement of mRNA levels of IbA1, GFAP and C3 as markers of microglial reactivity, astrocyte reactivity and complement activation, respectively.

    Article Snippet: For knock-down analysis on tissue sections using in situ hybridization (RNAScope, ACD), left cerebral hemisphere half-slices were cut into one ventral and one dorsal parts and immediately immersed in 10% neutral buffer formalin (NBF) fixative solution for approximately 48h at RT.

    Techniques: Clinical Proteomics, Injection, Knockdown, Quantitative RT-PCR, In Situ Hybridization, RNAscope, Activation Assay