Membrane:Article Title: Alpha-Lipoic Acid Inhibits Spontaneous Diabetes and Autoimmune Recurrence in Non-Obese Diabetic Mice by Enhancing Differentiation of Regulatory T Cells and Showed Potential for Use in Cell Therapies for the Treatment of Type 1 Diabetes
Article Snippet: The membrane was blocked with 5% skim milk at room temperature for 1 h and was then incubated in a buffer with a rabbit anti-STAT5 antibody (GeneTex Inc., Alton Pkwy Irvine, CA, USA), anti-acetylated H3 (GeneTex Inc.) or a mouse anti-β-actin antibody (GeneTex Inc.) overnight. .. After washing with PBST (0.05% Tween20 in PBS) three times, the membrane was incubated in the hybridization buffer with a horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:2000; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) or a HRP-conjugated goat anti-mouse IgG antibody (Santa Cruz Biotechnology, Inc.) for 1 h. The membrane was subsequently washed with PBST three times. .. After incubation with the chemiluminescent HRP substrate (Millipore), the signals were detected by the LAS-3000 imaging system (Fujifilm, Tokyo, Japan).
Incubation:Article Title: Alpha-Lipoic Acid Inhibits Spontaneous Diabetes and Autoimmune Recurrence in Non-Obese Diabetic Mice by Enhancing Differentiation of Regulatory T Cells and Showed Potential for Use in Cell Therapies for the Treatment of Type 1 Diabetes
Article Snippet: The membrane was blocked with 5% skim milk at room temperature for 1 h and was then incubated in a buffer with a rabbit anti-STAT5 antibody (GeneTex Inc., Alton Pkwy Irvine, CA, USA), anti-acetylated H3 (GeneTex Inc.) or a mouse anti-β-actin antibody (GeneTex Inc.) overnight. .. After washing with PBST (0.05% Tween20 in PBS) three times, the membrane was incubated in the hybridization buffer with a horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:2000; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) or a HRP-conjugated goat anti-mouse IgG antibody (Santa Cruz Biotechnology, Inc.) for 1 h. The membrane was subsequently washed with PBST three times. .. After incubation with the chemiluminescent HRP substrate (Millipore), the signals were detected by the LAS-3000 imaging system (Fujifilm, Tokyo, Japan).
Article Title: Stress Granule Induction in Rat Retinas Damaged by Constant LED Light
Article Snippet: Subsequently, they were hybridized with Cy3-Oligo(dT) 30 (Sigma-Aldrich Corp.) diluted in hybridization buffer, at a final concentration of 20 nM, ON at 40°C in a humidified chamber. .. Then, samples were rinsed once with Washing Buffer 1 (50% [v/v] formamide 12.6 M, 0.25 M NaCl, 0.075 M Tris Buffer [pH 8.5], and 0.1% [v/v] Tween 20) five minutes at RT in constant shaking, four times with washing buffer 2 (0.05 M NaCl, 0.075 M Tris Buffer [pH 8.5] and 0.1% [v/v] Tween 20) for 15 minutes each in constant shaking and with TBS 5 min. Then, they were blocked with blocking buffer for 2.5 h at RT with continuous shaking and incubated overnight with anti-eIF3 (sc-16377; Santa Cruz Biotechnology) diluted in blocking buffer at 4°C in a humidified chamber. ..
Article Title: IL-1 and TNF mediates IL-6 signaling at the maternal-fetal interface during intrauterine inflammation
Article Snippet: .. After In situ hybridization step, the slides were subjected to immunofluorescence staining to co-localize different proteins by incubation with either anti-human vimentin (Cat.# SC7557; dilution 1:200, Santa Cruz), Pancytokeratin (Cat.#SC81714; dilution 1:100, Santa Cruz), CD45 (CD45–2B11; Cat# 14–9457-82; dilution 1:50, Thermofisher) in 10% normal horse serum/0.2% Tween 20 at 4°C overnight. .. Staining was visualized using fluorescently labeled secondary antibodies (AF488; 1:200 dilution; Invitrogen) for 1 hour at room temperature.
Hybridization:Article Title: Alpha-Lipoic Acid Inhibits Spontaneous Diabetes and Autoimmune Recurrence in Non-Obese Diabetic Mice by Enhancing Differentiation of Regulatory T Cells and Showed Potential for Use in Cell Therapies for the Treatment of Type 1 Diabetes
Article Snippet: The membrane was blocked with 5% skim milk at room temperature for 1 h and was then incubated in a buffer with a rabbit anti-STAT5 antibody (GeneTex Inc., Alton Pkwy Irvine, CA, USA), anti-acetylated H3 (GeneTex Inc.) or a mouse anti-β-actin antibody (GeneTex Inc.) overnight. .. After washing with PBST (0.05% Tween20 in PBS) three times, the membrane was incubated in the hybridization buffer with a horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:2000; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) or a HRP-conjugated goat anti-mouse IgG antibody (Santa Cruz Biotechnology, Inc.) for 1 h. The membrane was subsequently washed with PBST three times. .. After incubation with the chemiluminescent HRP substrate (Millipore), the signals were detected by the LAS-3000 imaging system (Fujifilm, Tokyo, Japan).
Blocking Assay:Article Title: Stress Granule Induction in Rat Retinas Damaged by Constant LED Light
Article Snippet: Subsequently, they were hybridized with Cy3-Oligo(dT) 30 (Sigma-Aldrich Corp.) diluted in hybridization buffer, at a final concentration of 20 nM, ON at 40°C in a humidified chamber. .. Then, samples were rinsed once with Washing Buffer 1 (50% [v/v] formamide 12.6 M, 0.25 M NaCl, 0.075 M Tris Buffer [pH 8.5], and 0.1% [v/v] Tween 20) five minutes at RT in constant shaking, four times with washing buffer 2 (0.05 M NaCl, 0.075 M Tris Buffer [pH 8.5] and 0.1% [v/v] Tween 20) for 15 minutes each in constant shaking and with TBS 5 min. Then, they were blocked with blocking buffer for 2.5 h at RT with continuous shaking and incubated overnight with anti-eIF3 (sc-16377; Santa Cruz Biotechnology) diluted in blocking buffer at 4°C in a humidified chamber. ..
In Situ Hybridization:Article Title: IL-1 and TNF mediates IL-6 signaling at the maternal-fetal interface during intrauterine inflammation
Article Snippet: .. After In situ hybridization step, the slides were subjected to immunofluorescence staining to co-localize different proteins by incubation with either anti-human vimentin (Cat.# SC7557; dilution 1:200, Santa Cruz), Pancytokeratin (Cat.#SC81714; dilution 1:100, Santa Cruz), CD45 (CD45–2B11; Cat# 14–9457-82; dilution 1:50, Thermofisher) in 10% normal horse serum/0.2% Tween 20 at 4°C overnight. .. Staining was visualized using fluorescently labeled secondary antibodies (AF488; 1:200 dilution; Invitrogen) for 1 hour at room temperature.
Article Title: FOXM1-induced miR-552 expression contributes to pancreatic cancer progression by targeting multiple tumor suppressor genes
Article Snippet: .. Immunohistochemical staining for FOXM1 and in situ hybridization for miR-552 were respectively performed according to the commercial protocol (Outdo Biotech, Shanghai, China) using anti-FOXM1 antibody (Santa Cruz Biotechnology, USA) or 3' and 5' DIG labeled LNA miR-552 probes (Ambion, USA). .. Immunohistochemical staining for FOXM1 and in situ hybridization for miR-552 were respectively performed according to the commercial protocol (Outdo Biotech, Shanghai, China) using anti-FOXM1 antibody (Santa Cruz Biotechnology, USA) or 3' and 5' DIG labeled LNA miR-552 probes (Ambion, USA).
Article Title: LATS1/2 inactivation in the mammary epithelium drives the evolution of a tumor-associated niche.
Article Snippet: .. Immunofluorescence, immunohistochemistry, and RNAscope in situ hybridization The fourth mammary glands were harvested from animals and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, # 15710 or Santa Cruz Biotechnology SC281692) overnight, then dehydrated and embedded in paraffin (Leica Microsystems 39602004). .. Immunofluorescence, immunohistochemistry, and RNAscope in situ hybridization The fourth mammary glands were harvested from animals and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, # 15710 or Santa Cruz Biotechnology SC281692) overnight, then dehydrated and embedded in paraffin (Leica Microsystems 39602004).
Immunofluorescence:Article Title: IL-1 and TNF mediates IL-6 signaling at the maternal-fetal interface during intrauterine inflammation
Article Snippet: .. After In situ hybridization step, the slides were subjected to immunofluorescence staining to co-localize different proteins by incubation with either anti-human vimentin (Cat.# SC7557; dilution 1:200, Santa Cruz), Pancytokeratin (Cat.#SC81714; dilution 1:100, Santa Cruz), CD45 (CD45–2B11; Cat# 14–9457-82; dilution 1:50, Thermofisher) in 10% normal horse serum/0.2% Tween 20 at 4°C overnight. .. Staining was visualized using fluorescently labeled secondary antibodies (AF488; 1:200 dilution; Invitrogen) for 1 hour at room temperature.
Article Title: LATS1/2 inactivation in the mammary epithelium drives the evolution of a tumor-associated niche.
Article Snippet: .. Immunofluorescence, immunohistochemistry, and RNAscope in situ hybridization The fourth mammary glands were harvested from animals and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, # 15710 or Santa Cruz Biotechnology SC281692) overnight, then dehydrated and embedded in paraffin (Leica Microsystems 39602004). .. Immunofluorescence, immunohistochemistry, and RNAscope in situ hybridization The fourth mammary glands were harvested from animals and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, # 15710 or Santa Cruz Biotechnology SC281692) overnight, then dehydrated and embedded in paraffin (Leica Microsystems 39602004).
Staining:Article Title: IL-1 and TNF mediates IL-6 signaling at the maternal-fetal interface during intrauterine inflammation
Article Snippet: .. After In situ hybridization step, the slides were subjected to immunofluorescence staining to co-localize different proteins by incubation with either anti-human vimentin (Cat.# SC7557; dilution 1:200, Santa Cruz), Pancytokeratin (Cat.#SC81714; dilution 1:100, Santa Cruz), CD45 (CD45–2B11; Cat# 14–9457-82; dilution 1:50, Thermofisher) in 10% normal horse serum/0.2% Tween 20 at 4°C overnight. .. Staining was visualized using fluorescently labeled secondary antibodies (AF488; 1:200 dilution; Invitrogen) for 1 hour at room temperature.
Article Title: FOXM1-induced miR-552 expression contributes to pancreatic cancer progression by targeting multiple tumor suppressor genes
Article Snippet: .. Immunohistochemical staining for FOXM1 and in situ hybridization for miR-552 were respectively performed according to the commercial protocol (Outdo Biotech, Shanghai, China) using anti-FOXM1 antibody (Santa Cruz Biotechnology, USA) or 3' and 5' DIG labeled LNA miR-552 probes (Ambion, USA). .. Immunohistochemical staining for FOXM1 and in situ hybridization for miR-552 were respectively performed according to the commercial protocol (Outdo Biotech, Shanghai, China) using anti-FOXM1 antibody (Santa Cruz Biotechnology, USA) or 3' and 5' DIG labeled LNA miR-552 probes (Ambion, USA).
Article Title: The latency-associated transcript locus of herpes simplex virus 1 is a virulence determinant in human skin.
Article Snippet: Alternate 6 μm tissue sections were stained with haematoxylin and eosin (H&E), HSV DNA BIO-PROBE (Enzo cat. # ENZ-40838) or LAT ATD19 RNA probe [73] by in situ hybridisation, and by antibodies. .. Antigen retrieval using a citrate buffer in a pressure cooker was performed prior to staining sections with antibodies to the viral ICP0 and cellular PML proteins (Santa Cruz HSV-1 ICP0 Antibody (11060) mouse monoclonal IgG2b cat. # sc-53070 lot # A0313 and PML Antibody (H-238) rabbit polyclonal IgG cat. # sc-5621 lot # J2010). .. H&E staining and in situ hybridisation images were captured using a Leica DM2000 with PL FLUOTAR 10X/0.3 (cat. # 506505) lens and camera DFC290HD.
Immunohistochemical staining:Article Title: FOXM1-induced miR-552 expression contributes to pancreatic cancer progression by targeting multiple tumor suppressor genes
Article Snippet: .. Immunohistochemical staining for FOXM1 and in situ hybridization for miR-552 were respectively performed according to the commercial protocol (Outdo Biotech, Shanghai, China) using anti-FOXM1 antibody (Santa Cruz Biotechnology, USA) or 3' and 5' DIG labeled LNA miR-552 probes (Ambion, USA). .. Immunohistochemical staining for FOXM1 and in situ hybridization for miR-552 were respectively performed according to the commercial protocol (Outdo Biotech, Shanghai, China) using anti-FOXM1 antibody (Santa Cruz Biotechnology, USA) or 3' and 5' DIG labeled LNA miR-552 probes (Ambion, USA).
Labeling:Article Title: FOXM1-induced miR-552 expression contributes to pancreatic cancer progression by targeting multiple tumor suppressor genes
Article Snippet: .. Immunohistochemical staining for FOXM1 and in situ hybridization for miR-552 were respectively performed according to the commercial protocol (Outdo Biotech, Shanghai, China) using anti-FOXM1 antibody (Santa Cruz Biotechnology, USA) or 3' and 5' DIG labeled LNA miR-552 probes (Ambion, USA). .. Immunohistochemical staining for FOXM1 and in situ hybridization for miR-552 were respectively performed according to the commercial protocol (Outdo Biotech, Shanghai, China) using anti-FOXM1 antibody (Santa Cruz Biotechnology, USA) or 3' and 5' DIG labeled LNA miR-552 probes (Ambion, USA).
Immunohistochemistry:Article Title: LATS1/2 inactivation in the mammary epithelium drives the evolution of a tumor-associated niche.
Article Snippet: .. Immunofluorescence, immunohistochemistry, and RNAscope in situ hybridization The fourth mammary glands were harvested from animals and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, # 15710 or Santa Cruz Biotechnology SC281692) overnight, then dehydrated and embedded in paraffin (Leica Microsystems 39602004). .. Immunofluorescence, immunohistochemistry, and RNAscope in situ hybridization The fourth mammary glands were harvested from animals and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, # 15710 or Santa Cruz Biotechnology SC281692) overnight, then dehydrated and embedded in paraffin (Leica Microsystems 39602004).
RNAscope:Article Title: LATS1/2 inactivation in the mammary epithelium drives the evolution of a tumor-associated niche.
Article Snippet: .. Immunofluorescence, immunohistochemistry, and RNAscope in situ hybridization The fourth mammary glands were harvested from animals and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, # 15710 or Santa Cruz Biotechnology SC281692) overnight, then dehydrated and embedded in paraffin (Leica Microsystems 39602004). .. Immunofluorescence, immunohistochemistry, and RNAscope in situ hybridization The fourth mammary glands were harvested from animals and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, # 15710 or Santa Cruz Biotechnology SC281692) overnight, then dehydrated and embedded in paraffin (Leica Microsystems 39602004).
Electron Microscopy:Article Title: LATS1/2 inactivation in the mammary epithelium drives the evolution of a tumor-associated niche.
Article Snippet: .. Immunofluorescence, immunohistochemistry, and RNAscope in situ hybridization The fourth mammary glands were harvested from animals and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, # 15710 or Santa Cruz Biotechnology SC281692) overnight, then dehydrated and embedded in paraffin (Leica Microsystems 39602004). .. Immunofluorescence, immunohistochemistry, and RNAscope in situ hybridization The fourth mammary glands were harvested from animals and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, # 15710 or Santa Cruz Biotechnology SC281692) overnight, then dehydrated and embedded in paraffin (Leica Microsystems 39602004).
|