Cell Culture:
Article Title: A versatile multiplexed assay to quantify intracellular ROS and cell viability in 3D on-a-chip models.
Article Snippet: Primary human umbilical vein endothelial cells (HUVECs from Lonza, #C2519AS), EBM-2 medium (Lonza, #CC-3156), supplemented with EGM-2 SingleQuots (Lonza, #CC-4176), Human epithelial colorectal adenocarcinoma cells (Caco2 from Sigma-Aldrich, #86010202), rat tail collagen I (Corning, #354249), Acetic acid (Sigma-Aldrich, #A6283, ≥99%), HEPES buffer saline solution (Lonza, #CC-5024), sodium bicarbonate (NaHCO3 from Sigma-Aldrich, #S5761), Hanks’ Balanced Salt Solution (HBSS from Gibco, #55037C), PhosphateBuffered Saline (PBS from Gibco, #70013065), Vascular Endothelial Growth Factor (VEGF rhVEGF-164 PeproTech, #100-20-1 mg), Sphingosine-1-phospate (S1P from Sigma-Aldrich, #73914), Phorbol myristate acetate (PMA from Sigma Aldrich, #P1585-1 MG), Dimethylsulfoxide (DMSO from Sigma Aldrich, #D8418), Ethanol absolute (VWR, #BAKR8025.2500), MilliQ water (Obtained with Millipore Vent filter 12FC ZFRE012FC), tert-butyl hydroperoxide solution (TBHP from Sigma, #458139, 70% in H2O w/w), Dihydrorhodamine 123 (DHR123 from Sigma-Aldrich, #D1054), Hoechst 33342 (ThermoFisher, #H3570, 10 mg/mL in H2O), Propidium Iodide (PI from ThermoFisher, #P3566, 1 mg/mL in H2O), 3,4,5-Trihydroxybenzoic Acid Hydrate (Gallic acid hydrate from TCI, #G0011), 3,4′,5-Trihydroxy-trans-stilbene (Resveratrol from Sigma Aldrich, #R5010), (E,E)-1,7-Bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione (Curcumin from TCI, #C0434), (±)-α-Tocopherol (Sigma Aldrich, #T3251), 4-Amino-5-methylamino2′,7′-difluorofluorescein diacetate (DAF-FM DA from Sigma-Aldrich, #D2321, 5 mM in DMSO), Rotenone (Sigma-Aldrich, #R8875), Antimycin A from Streptomyces sp. (Sigma-Aldrich, #A8674).
Article Title: A versatile multiplexed assay to quantify intracellular ROS and cell viability in 3D on-a-chip models
Article Snippet: Primary human umbilical vein endothelial cells (HUVECs from Lonza, #C2519AS), EBM-2 medium (Lonza, #CC-3156), supplemented with EGM-2 SingleQuots (Lonza, #CC-4176), Human epithelial colorectal adenocarcinoma cells (Caco2 from Sigma-Aldrich, #86010202), rat tail collagen I (Corning, #354249), Acetic acid (Sigma-Aldrich, #A6283, ≥99%), HEPES buffer saline solution (Lonza, #CC-5024), sodium bicarbonate (NaHCO 3 from Sigma-Aldrich, #S5761), Hanks' Balanced Salt Solution (HBSS from Gibco, #55037C), Phosphate-Buffered Saline (PBS from Gibco, #70013065), Vascular Endothelial Growth Factor (VEGF rhVEGF-164 PeproTech, #100-20-1 mg), Sphingosine-1-phospate (S1P from Sigma-Aldrich, #73914), Phorbol myristate acetate (PMA from Sigma Aldrich, #P1585-1 MG), Dimethylsulfoxide (DMSO from Sigma Aldrich, #D8418), Ethanol absolute (VWR, #BAKR8025.2500), MilliQ water (Obtained with Millipore Vent filter 12FC ZFRE012FC), tert -butyl hydroperoxide solution (TBHP from Sigma, #458139, 70% in H2O w/w), Dihydrorhodamine 123 (DHR123 from Sigma-Aldrich, #D1054), Hoechst 33342 (ThermoFisher, #H3570, 10 mg/mL in H2O), Propidium Iodide (PI from ThermoFisher, #P3566, 1 mg/mL in H2O), 3,4,5-Trihydroxybenzoic Acid Hydrate (Gallic acid hydrate from TCI, #G0011), 3,4′,5-Trihydroxy-trans-stilbene (Resveratrol from Sigma Aldrich, #R5010), (E,E)-1,7-Bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione (Curcumin from TCI, #C0434), (±)-α-Tocopherol (Sigma Aldrich, #T3251), 4-Amino-5-methylamino- 2′,7′-difluorofluorescein diacetate (DAF-FM DA from Sigma-Aldrich, #D2321, 5 mM in DMSO), Rotenone (Sigma-Aldrich, #R8875), Antimycin A from Streptomyces sp. (Sigma-Aldrich, #A8674).
Article Title: Characterization of 3D heterocellular spheroids of pancreatic ductal adenocarcinoma for the study of cell interactions in the tumor immune microenvironment
Article Snippet: Human umbilical vein endothelial cells (HUVEC, Lonza, C2519AS, Lot#633426) and RFP-HUVEC (Angioproteomie cAP-001RFP, Lot#2021122802) were cultured in EGM-2TM SingleQuotTM containing 0.5 ng/ml VEGF, 5 ng/ml EGF, 10 ng/ml bFGF, 20 ng/ml long R3-IGF-1, 22.5 μg/ml heparin, 1 μg/ml ascorbic acid, 0.2 μg/ml hydrocortisone, gentamicin (1/1000 dilution) and 2% FBS.
Article Title: Characterization of 3D heterocellular spheroids of pancreatic ductal adenocarcinoma for the study of cell interactions in the tumor immune microenvironment.
Article Snippet: Human umbilical vein endothelial cells (HUVEC, Lonza, C2519AS, Lot#633426) and RFP-HUVEC (Angioproteomie cAP-001RFP, Lot#2021122802) were cultured in EGM-2TM SingleQuotTM containing 0.5 ng/ml VEGF, 5 ng/ml EGF, 10 ng/ml bFGF, 20 ng/ml long R3-IGF-1, 22.5 mg/ml heparin, 1 mg/ml ascorbic acid, 0.2 mg/ml hydrocortisone, gentamicin (1/1000 dilution) and 2% FBS.
Confocal Microscopy:
Article Title: A versatile multiplexed assay to quantify intracellular ROS and cell viability in 3D on-a-chip models.
Article Snippet: Primary human umbilical vein endothelial cells (HUVECs from Lonza, #C2519AS), EBM-2 medium (Lonza, #CC-3156), supplemented with EGM-2 SingleQuots (Lonza, #CC-4176), Human epithelial colorectal adenocarcinoma cells (Caco2 from Sigma-Aldrich, #86010202), rat tail collagen I (Corning, #354249), Acetic acid (Sigma-Aldrich, #A6283, ≥99%), HEPES buffer saline solution (Lonza, #CC-5024), sodium bicarbonate (NaHCO3 from Sigma-Aldrich, #S5761), Hanks’ Balanced Salt Solution (HBSS from Gibco, #55037C), PhosphateBuffered Saline (PBS from Gibco, #70013065), Vascular Endothelial Growth Factor (VEGF rhVEGF-164 PeproTech, #100-20-1 mg), Sphingosine-1-phospate (S1P from Sigma-Aldrich, #73914), Phorbol myristate acetate (PMA from Sigma Aldrich, #P1585-1 MG), Dimethylsulfoxide (DMSO from Sigma Aldrich, #D8418), Ethanol absolute (VWR, #BAKR8025.2500), MilliQ water (Obtained with Millipore Vent filter 12FC ZFRE012FC), tert-butyl hydroperoxide solution (TBHP from Sigma, #458139, 70% in H2O w/w), Dihydrorhodamine 123 (DHR123 from Sigma-Aldrich, #D1054), Hoechst 33342 (ThermoFisher, #H3570, 10 mg/mL in H2O), Propidium Iodide (PI from ThermoFisher, #P3566, 1 mg/mL in H2O), 3,4,5-Trihydroxybenzoic Acid Hydrate (Gallic acid hydrate from TCI, #G0011), 3,4′,5-Trihydroxy-trans-stilbene (Resveratrol from Sigma Aldrich, #R5010), (E,E)-1,7-Bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione (Curcumin from TCI, #C0434), (±)-α-Tocopherol (Sigma Aldrich, #T3251), 4-Amino-5-methylamino2′,7′-difluorofluorescein diacetate (DAF-FM DA from Sigma-Aldrich, #D2321, 5 mM in DMSO), Rotenone (Sigma-Aldrich, #R8875), Antimycin A from Streptomyces sp. (Sigma-Aldrich, #A8674).
Article Title: A versatile multiplexed assay to quantify intracellular ROS and cell viability in 3D on-a-chip models
Article Snippet: Primary human umbilical vein endothelial cells (HUVECs from Lonza, #C2519AS), EBM-2 medium (Lonza, #CC-3156), supplemented with EGM-2 SingleQuots (Lonza, #CC-4176), Human epithelial colorectal adenocarcinoma cells (Caco2 from Sigma-Aldrich, #86010202), rat tail collagen I (Corning, #354249), Acetic acid (Sigma-Aldrich, #A6283, ≥99%), HEPES buffer saline solution (Lonza, #CC-5024), sodium bicarbonate (NaHCO 3 from Sigma-Aldrich, #S5761), Hanks' Balanced Salt Solution (HBSS from Gibco, #55037C), Phosphate-Buffered Saline (PBS from Gibco, #70013065), Vascular Endothelial Growth Factor (VEGF rhVEGF-164 PeproTech, #100-20-1 mg), Sphingosine-1-phospate (S1P from Sigma-Aldrich, #73914), Phorbol myristate acetate (PMA from Sigma Aldrich, #P1585-1 MG), Dimethylsulfoxide (DMSO from Sigma Aldrich, #D8418), Ethanol absolute (VWR, #BAKR8025.2500), MilliQ water (Obtained with Millipore Vent filter 12FC ZFRE012FC), tert -butyl hydroperoxide solution (TBHP from Sigma, #458139, 70% in H2O w/w), Dihydrorhodamine 123 (DHR123 from Sigma-Aldrich, #D1054), Hoechst 33342 (ThermoFisher, #H3570, 10 mg/mL in H2O), Propidium Iodide (PI from ThermoFisher, #P3566, 1 mg/mL in H2O), 3,4,5-Trihydroxybenzoic Acid Hydrate (Gallic acid hydrate from TCI, #G0011), 3,4′,5-Trihydroxy-trans-stilbene (Resveratrol from Sigma Aldrich, #R5010), (E,E)-1,7-Bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione (Curcumin from TCI, #C0434), (±)-α-Tocopherol (Sigma Aldrich, #T3251), 4-Amino-5-methylamino- 2′,7′-difluorofluorescein diacetate (DAF-FM DA from Sigma-Aldrich, #D2321, 5 mM in DMSO), Rotenone (Sigma-Aldrich, #R8875), Antimycin A from Streptomyces sp. (Sigma-Aldrich, #A8674).
Article Title: Characterization of 3D heterocellular spheroids of pancreatic ductal adenocarcinoma for the study of cell interactions in the tumor immune microenvironment
Article Snippet: Human umbilical vein endothelial cells (HUVEC, Lonza, C2519AS, Lot#633426) and RFP-HUVEC (Angioproteomie cAP-001RFP, Lot#2021122802) were cultured in EGM-2TM SingleQuotTM containing 0.5 ng/ml VEGF, 5 ng/ml EGF, 10 ng/ml bFGF, 20 ng/ml long R3-IGF-1, 22.5 μg/ml heparin, 1 μg/ml ascorbic acid, 0.2 μg/ml hydrocortisone, gentamicin (1/1000 dilution) and 2% FBS.
Article Title: Characterization of 3D heterocellular spheroids of pancreatic ductal adenocarcinoma for the study of cell interactions in the tumor immune microenvironment.
Article Snippet: Human umbilical vein endothelial cells (HUVEC, Lonza, C2519AS, Lot#633426) and RFP-HUVEC (Angioproteomie cAP-001RFP, Lot#2021122802) were cultured in EGM-2TM SingleQuotTM containing 0.5 ng/ml VEGF, 5 ng/ml EGF, 10 ng/ml bFGF, 20 ng/ml long R3-IGF-1, 22.5 mg/ml heparin, 1 mg/ml ascorbic acid, 0.2 mg/ml hydrocortisone, gentamicin (1/1000 dilution) and 2% FBS.
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