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PromoCell human umbilical vein endothelial cells huvecs
Human Umbilical Vein Endothelial Cells Huvecs, supplied by PromoCell, used in various techniques. Bioz Stars score: 98/100, based on 1580 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/huvec/Human+Umbilical+Vein+Endothelial+Cells+(HUVEC)+single+donor/pm42243163-46-0-13
Average 98 stars, based on 1580 article reviews

human umbilical vein endothelial cells huvecs - by Bioz Stars, 2026-11

98/100 stars

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Cell Culture:

Article Title: p66Shc mediates SUMO2-induced endothelial dysfunction
Article Snippet: .. Human umbilical vein endothelial cells (HUVECs) were purchased from PromoCell (Heidelberg, Germany) and Lonza (Walkersville, MD) and were cultured in endothelial growth medium-2 from PromoCell and Lonza. .. HEK293 cells were cultured in DMEM (Gibco) supplemented with 10% fetal bovine serum (Thermo) and 1% Pen Strep (penicillin 10,000 units/ml and 10,000 ug/ml streptomycin; Gibco).

Article Title: Simulated microgravity induces a NOX-sensitive oxidative response that is attenuated by resveratrol in human endothelial cells.
Article Snippet: .. Human umbilical vein endothelial cells (HUVECs) were purchased as cryopreserved primary cells from PromoCell (Heidelberg, Germany) and cultured according to previously described protocols (Giordo, Posadino et al. 2024). ..

Article Title: 3D co-culture model of tumor spheroid and endothelial cells unveils ccRCC aberrant vasculature and distinct sensitivity to targeted treatments.
Article Snippet: Extended author information available on the last page of the article Abstract Clear cell renal cell carcinoma (ccRCC), the most common renal cancer, is largely driven by von Hippel Lindau (VHL) protein deficiency.. VHL inactivation promotes epithelial-to-mesenchymal transition (EMT), invasion, and hypervascularization, through vascular endothelial growth factor (VEGF) signaling, resulting in an abnormally complex vasculature.. Given the limited prognostic value of microvessel density and the frequent development of resistance to VEGF-targeted therapies, the architecture of the ccRCC vascular network is likely a critical, underexplored determinant of therapeutic response.

Article Title: High-throughput screen identifies metabolite regulators of endothelial function
Article Snippet: .. HUVECs (C-12200 and C-12203, PromoCell), HCAECs (C-12221, PromoCell) and HPMECs (C-12281, PromoCell) were cultured from passages 3 to 10 under physiologic normoxia at 3% O2, since physiological levels of oxygen in the body are between 2–9%66. .. HUVECs were cultured on plates coated with 0.5% gelatine (48,723, Sigma-Aldrich) in endothelial cell growth medium (ECGM): Ham’s F12 (21,765,037, Gibco) supplemented with 10% FBS (S0615, Sigma-Aldrich), 25 μg/ml Heparin (A3004, AppliChem PanReac), 1 μg/ml Ascorbic acid (A4403, Sigma-Aldrich), 2 mM GlutaMax (35,050,038, Gibco), 100 U/ml Penicillin–Streptomycin (15,140,122, Gibco) and Endothelial cell growth supplement (02–102, Millipore).

Article Title: A Versatile Microfluidic Extrusion-Based Hydrogel Platform for Self-Organization and Long-Term Maintenance of Engineered 3D Lymphatic Endothelium.
Article Snippet: Materials andMethods .1 Cell Culture rimary human dermal lymphatic endothelial cells (HDLECs; romoCell, C-12216) and human dermal microvascular endotheial cells (HDMECs; PromoCell, C-12212) were cultured in ndothelial Cell Growth Medium MV2 (EGM2-MV; PromoCell, -22022). .. Human umbilical vein endothelial cells (HUVECs; onza, CC-2519) were cultured in Endothelial Cell Growth edium 2 (EGM2; PromoCell, C-22011). .. 0 of 16 All media were supplemented with 1% penicillin–streptomycin (Gibco, 15140122).

Isolation:

Image Search Results

(A) Schematic of the three-dimensional inflammation-on-a-chip device. A cylindrical lumen lined with human umbilical vein endothelial cells sits within a collagen–fibronectin extracellular matrix. Primary human monocyte-derived macrophages are polarized in situ within the matrix toward pro-inflammatory (Pro-) or anti-inflammatory (Anti-) phenotypes. Top and side ports allow injection of chemoattractants, neutrophils and inhibitors. (B) Cell viability (%) for pro- and anti-inflammatory macrophages on Days 6, 7 and 9. N = 3, n = 3. (C) Polarization Index (CD163 + /CD80 + ratio, log scale). Welch’s t-test on log 2 -transformed values, P = 0.007. N = 4–6, n = 3. (D) Anti/Pro cytokine secretion ratio (log 2 ) for pro- and anti-inflammatory macrophages in 3D collagen– fibronectin hydrogels, quantified by multiplex MagPix Luminex assay. The ratio is the summed concentrations of the three anti-inflammatory analytes (IL-10, soluble CD163 and MDC/CCL22) divided by the summed concentrations of the two pro-inflammatory analytes (IL-6 and TNF-α). Welch’s t-test on log 2 -transformed values, P = 0.0275. N = 4– 5, n = 1. (E) Representative confocal maximum-intensity projections of anti-inflammatory (top) and pro-inflammatory (bottom) macrophages in the 3D collagen–fibronectin matrix on Day 9, stained for Hoechst (nuclei, blue), CD68 (white), CD80 (red) and CD163 (green). Scale bar, 100 µm. In (B) to (D), the center line is the median, the box spans the interquartile range and the whiskers span the full rang(E) with individual biological replicates overlaid.

Journal: bioRxiv

Article Title: Anti-inflammatory macrophages limit endothelial inflammation and restrict neutrophil recruitment through Gas6/AXL signaling

doi: 10.64898/2026.10.12.758565

Figure Lengend Snippet: (A) Schematic of the three-dimensional inflammation-on-a-chip device. A cylindrical lumen lined with human umbilical vein endothelial cells sits within a collagen–fibronectin extracellular matrix. Primary human monocyte-derived macrophages are polarized in situ within the matrix toward pro-inflammatory (Pro-) or anti-inflammatory (Anti-) phenotypes. Top and side ports allow injection of chemoattractants, neutrophils and inhibitors. (B) Cell viability (%) for pro- and anti-inflammatory macrophages on Days 6, 7 and 9. N = 3, n = 3. (C) Polarization Index (CD163 + /CD80 + ratio, log scale). Welch’s t-test on log 2 -transformed values, P = 0.007. N = 4–6, n = 3. (D) Anti/Pro cytokine secretion ratio (log 2 ) for pro- and anti-inflammatory macrophages in 3D collagen– fibronectin hydrogels, quantified by multiplex MagPix Luminex assay. The ratio is the summed concentrations of the three anti-inflammatory analytes (IL-10, soluble CD163 and MDC/CCL22) divided by the summed concentrations of the two pro-inflammatory analytes (IL-6 and TNF-α). Welch’s t-test on log 2 -transformed values, P = 0.0275. N = 4– 5, n = 1. (E) Representative confocal maximum-intensity projections of anti-inflammatory (top) and pro-inflammatory (bottom) macrophages in the 3D collagen–fibronectin matrix on Day 9, stained for Hoechst (nuclei, blue), CD68 (white), CD80 (red) and CD163 (green). Scale bar, 100 µm. In (B) to (D), the center line is the median, the box spans the interquartile range and the whiskers span the full rang(E) with individual biological replicates overlaid.

Article Snippet: Pooled primary human umbilical vein endothelial cells (HUVECs; PromoCell, C-12203 and 50305964) were maintained in Endothelial Growth Medium-2 (EGM-2; Lonza, NC9525043) in T-75 flasks between passages 2 and 7, with media refreshed every 2 days.

Techniques: Derivative Assay, In Situ, Injection, Transformation Assay, Multiplex Assay, Luminex, Confocal, Staining

(A) Schematic of the FITC-dextran permeability assay. A 70-kDa FITC-dextran solution is perfused into the HUVEC lumen and live-imaged at the vessel midplane by confocal microscopy, and the diffusive permeability coefficient Pd is computed as the slope of normalized extracellular matrix fluorescence accumulation over the 8-min window multiplied by the vessel-wall geometric factor . (B) Representative time-lapse confocal micrographs of FITC-dextran perfusion across the endothelial barrier at 2-min intervals (t = 0, 2, 4, 6 and 8 min). Red lines mark the vessel walls. Scale bar, 100 µm. (C) Condition mean of the normalized extracellular matrix fluorescence traces over the 8-min acquisition window. Solid lines, with stimulation. Dashed lines, without. (D) Diffusive permeability coefficient Pd (× 10 −6 cm/s). All six conditions were acquired within each experimental series, across independent biological replicates. Kruskal–Wallis test with Bonferroni-corrected pairwise post hoc comparisons. *P < 0.05. N = 7–9, n = 2–3. In (D), the center line is the median, the box spans the interquartile range and the whiskers span the full range, with individual biological replicates overlaid. (+) denotes inflammatory stimulation and (−) its absence.

Journal: bioRxiv

Article Title: Anti-inflammatory macrophages limit endothelial inflammation and restrict neutrophil recruitment through Gas6/AXL signaling

doi: 10.64898/2026.10.12.758565

Figure Lengend Snippet: (A) Schematic of the FITC-dextran permeability assay. A 70-kDa FITC-dextran solution is perfused into the HUVEC lumen and live-imaged at the vessel midplane by confocal microscopy, and the diffusive permeability coefficient Pd is computed as the slope of normalized extracellular matrix fluorescence accumulation over the 8-min window multiplied by the vessel-wall geometric factor . (B) Representative time-lapse confocal micrographs of FITC-dextran perfusion across the endothelial barrier at 2-min intervals (t = 0, 2, 4, 6 and 8 min). Red lines mark the vessel walls. Scale bar, 100 µm. (C) Condition mean of the normalized extracellular matrix fluorescence traces over the 8-min acquisition window. Solid lines, with stimulation. Dashed lines, without. (D) Diffusive permeability coefficient Pd (× 10 −6 cm/s). All six conditions were acquired within each experimental series, across independent biological replicates. Kruskal–Wallis test with Bonferroni-corrected pairwise post hoc comparisons. *P < 0.05. N = 7–9, n = 2–3. In (D), the center line is the median, the box spans the interquartile range and the whiskers span the full range, with individual biological replicates overlaid. (+) denotes inflammatory stimulation and (−) its absence.

Techniques: FITC-Dextran Permeability Assay, Confocal, Microscopy, Permeability, Fluorescence

( A ) Immunoblot for Shc and SUMO2 expression in HUVECs following p66Shc knockdown (si-p66Shc) and SUMO2 overexpression (Ad-HA-SUMO2). ( B, C ) Quantification of ROS (MitoSOX fluorescence) in HUVECs from A. *p<0.05; n=24–32; Student’s t -test. ( D ) Quantification of ROS in HUVECs following p66Shc knockdown (si-p66Shc) and SUMO2 overexpression (Ad-HA-SUMO2). ***p<0.001, p<0.05; n=10–15; One-way ANOVA. Data represents mean ± SEM. Immunoblots are representative of at least three independent experiments. GC, scrambled siRNA used as control; HUVECs, human umbilical vein endothelial cells. Figure 1—source data 1. PDF file containing original western blots for . Figure 1—source data 2. Original files for western blot analysis displayed in .

Journal: eLife

Article Title: p66Shc mediates SUMO2-induced endothelial dysfunction

doi: 10.7554/eLife.106344

Figure Lengend Snippet: ( A ) Immunoblot for Shc and SUMO2 expression in HUVECs following p66Shc knockdown (si-p66Shc) and SUMO2 overexpression (Ad-HA-SUMO2). ( B, C ) Quantification of ROS (MitoSOX fluorescence) in HUVECs from A. *p<0.05; n=24–32; Student’s t -test. ( D ) Quantification of ROS in HUVECs following p66Shc knockdown (si-p66Shc) and SUMO2 overexpression (Ad-HA-SUMO2). ***p<0.001, p<0.05; n=10–15; One-way ANOVA. Data represents mean ± SEM. Immunoblots are representative of at least three independent experiments. GC, scrambled siRNA used as control; HUVECs, human umbilical vein endothelial cells. Figure 1—source data 1. PDF file containing original western blots for . Figure 1—source data 2. Original files for western blot analysis displayed in .

Article Snippet: Human umbilical vein endothelial cells (HUVECs) were purchased from PromoCell (Heidelberg, Germany) and Lonza (Walkersville, MD) and were cultured in endothelial growth medium-2 from PromoCell and Lonza.

Techniques: Western Blot, Expressing, Knockdown, Over Expression, Fluorescence, Control, Analysis

( A ) Immunoblot for sumoylated p66Shc in HEK-293 cells overexpressing SUMO2 (HA-SUMO2) and p66Shc (Flag-p66Shc). ( B ) Immunoblot for p66Shc sumoylation in HEK-293 cells overexpressing SUMO2 (HA-SUMO2) and p66Shc (Flag-p66Shc) with or without overexpression of Ubc9 (HA-Ubc9) and SENP1 (Flag-SENP1). ( C ) Immunoblot for sumoylation of p66Shc in human umbilical vein endothelial cells (HUVECs) overexpressing SUMO2 (Ad-HA-SUMO2) or p66Shc (Ad-Flag-p66Shc). ( D ) Immunoblot for p66Shc sumoylation in HUVEC cells overexpressing SUMO2 (Ad-HA-SUMO2) or p66Shc (Ad-Flag-p66Shc) in the presence or absence of anacardic acid. Quantification of SUMO2-p66Shc from immunoblot of D. One-way ANOVA, followed by Tukey’s post-hoc analysis, n=4 *p<0.05, **p<0.01. ( F ) Immunoblot for sumoylated recombinant p66Shc in the presence of absence of Ubc9. Immunoblots are representative of at least three independent experiments. Data represents mean ± SEM. Ad-Lac Z was used as an adenoviral control to compensate for the difference in MOI among the groups. Figure 2—source data 1. PDF file containing original western blots for . Figure 2—source data 2. Original files for western blot analysis displayed in .

Journal: eLife

Article Title: p66Shc mediates SUMO2-induced endothelial dysfunction

doi: 10.7554/eLife.106344

Figure Lengend Snippet: ( A ) Immunoblot for sumoylated p66Shc in HEK-293 cells overexpressing SUMO2 (HA-SUMO2) and p66Shc (Flag-p66Shc). ( B ) Immunoblot for p66Shc sumoylation in HEK-293 cells overexpressing SUMO2 (HA-SUMO2) and p66Shc (Flag-p66Shc) with or without overexpression of Ubc9 (HA-Ubc9) and SENP1 (Flag-SENP1). ( C ) Immunoblot for sumoylation of p66Shc in human umbilical vein endothelial cells (HUVECs) overexpressing SUMO2 (Ad-HA-SUMO2) or p66Shc (Ad-Flag-p66Shc). ( D ) Immunoblot for p66Shc sumoylation in HUVEC cells overexpressing SUMO2 (Ad-HA-SUMO2) or p66Shc (Ad-Flag-p66Shc) in the presence or absence of anacardic acid. Quantification of SUMO2-p66Shc from immunoblot of D. One-way ANOVA, followed by Tukey’s post-hoc analysis, n=4 *p<0.05, **p<0.01. ( F ) Immunoblot for sumoylated recombinant p66Shc in the presence of absence of Ubc9. Immunoblots are representative of at least three independent experiments. Data represents mean ± SEM. Ad-Lac Z was used as an adenoviral control to compensate for the difference in MOI among the groups. Figure 2—source data 1. PDF file containing original western blots for . Figure 2—source data 2. Original files for western blot analysis displayed in .

Techniques: Western Blot, Over Expression, Analysis, Recombinant, Control

Immunoblot of human umbilical vein endothelial cells (HUVECs) transduced with wild-type p66Shc (Ad-Flag-p66ShcWT) and SUMO2 (Ad-HA-SUMO2) at the indicated concentrations. Immunoblots are representative of at least three independent experiments. Figure 2—figure supplement 1—source data 1. PDF file containing original western blots for . Figure 2—figure supplement 1—source data 2. Original files for western blot analysis displayed in .

Journal: eLife

Article Title: p66Shc mediates SUMO2-induced endothelial dysfunction

doi: 10.7554/eLife.106344

Figure Lengend Snippet: Immunoblot of human umbilical vein endothelial cells (HUVECs) transduced with wild-type p66Shc (Ad-Flag-p66ShcWT) and SUMO2 (Ad-HA-SUMO2) at the indicated concentrations. Immunoblots are representative of at least three independent experiments. Figure 2—figure supplement 1—source data 1. PDF file containing original western blots for . Figure 2—figure supplement 1—source data 2. Original files for western blot analysis displayed in .

Techniques: Western Blot, Transduction, Analysis

( A ) Modular structure of p66Shc showing conserved lysine-81 in the CH2 domain. ( B ) Tandem mass spectrometry of in vitro SUMO2ylated recombinant p66Shc. ( C ) Immunoblot showing sumoylation of p66Shc in HEK-293 cells overexpressing p66ShcWT (Flag-p66ShcWT) or p66ShcK81R (Flag-p66ShcK81R) with and without SUMO2 (HA-SUMO2). ( D ) Immunoblot showing sumoylation of p66Shc in human umbilical vein endothelial cells (HUVECs) overexpressing p66ShcWT (Ad-Flag-p66ShcWT) or p66ShcK81R (Ad-p66ShcK81R) with and without SUMO2 (Ad-HA-SUMO2). ( E ) Immunoblot for sumoylation of recombinant p66ShcWT or p66ShcK81R. ( F ) Immunoprecipitation of SUMO2-p66Shc using SUMO2-p66ShcK81 antibody or the matching IgG control in HUVECs followed by immunoblotting with SUMO2-p66ShcK81 antibody showing endogenous SUMO2ylation of p66Shc (left-side panel, the high-intensity IgG signal was masked with a tape which appears as a white strip). Immunoblotting with Shc antibody in whole-cell lysate (right-side panel). Immunoblots are representative of at least three independent experiments. CH2, collagen homology 2; PTB, phosphotyrosine binding; CH1, collagen homology 1; SH2, Src homology 2. Figure 3—source data 1. PDF file containing original western blots for . Figure 3—source data 2. Original files for western blot analysis displayed in . Figure 3—source data 3. PDF file containing original western blots for . Figure 3—source data 4. Original files for western blot analysis displayed in . Figure 3—source data 5. PDF file containing original western blots for . Figure 3—source data 6. Original files for western blot analysis displayed in . Figure 3—source data 7. PDF file containing original western blots for . Figure 3—source data 8. Original files for western blot analysis displayed in .

Journal: eLife

Article Title: p66Shc mediates SUMO2-induced endothelial dysfunction

doi: 10.7554/eLife.106344

Figure Lengend Snippet: ( A ) Modular structure of p66Shc showing conserved lysine-81 in the CH2 domain. ( B ) Tandem mass spectrometry of in vitro SUMO2ylated recombinant p66Shc. ( C ) Immunoblot showing sumoylation of p66Shc in HEK-293 cells overexpressing p66ShcWT (Flag-p66ShcWT) or p66ShcK81R (Flag-p66ShcK81R) with and without SUMO2 (HA-SUMO2). ( D ) Immunoblot showing sumoylation of p66Shc in human umbilical vein endothelial cells (HUVECs) overexpressing p66ShcWT (Ad-Flag-p66ShcWT) or p66ShcK81R (Ad-p66ShcK81R) with and without SUMO2 (Ad-HA-SUMO2). ( E ) Immunoblot for sumoylation of recombinant p66ShcWT or p66ShcK81R. ( F ) Immunoprecipitation of SUMO2-p66Shc using SUMO2-p66ShcK81 antibody or the matching IgG control in HUVECs followed by immunoblotting with SUMO2-p66ShcK81 antibody showing endogenous SUMO2ylation of p66Shc (left-side panel, the high-intensity IgG signal was masked with a tape which appears as a white strip). Immunoblotting with Shc antibody in whole-cell lysate (right-side panel). Immunoblots are representative of at least three independent experiments. CH2, collagen homology 2; PTB, phosphotyrosine binding; CH1, collagen homology 1; SH2, Src homology 2. Figure 3—source data 1. PDF file containing original western blots for . Figure 3—source data 2. Original files for western blot analysis displayed in . Figure 3—source data 3. PDF file containing original western blots for . Figure 3—source data 4. Original files for western blot analysis displayed in . Figure 3—source data 5. PDF file containing original western blots for . Figure 3—source data 6. Original files for western blot analysis displayed in . Figure 3—source data 7. PDF file containing original western blots for . Figure 3—source data 8. Original files for western blot analysis displayed in .

Techniques: Mass Spectrometry, In Vitro, Recombinant, Western Blot, Immunoprecipitation, Control, Stripping Membranes, Binding Assay, Analysis

( A ) Immunoblot for p66ShcS36 phosphorylation in HEK-293 cells overexpressing SUMO2 (HA-SUMO2) and p66ShcWT (Flag-p66ShcWT). ( B ) Quantification of p66ShcS36 phosphorylation from A. *p<0.05; n=6; one-way ANOVA. ( C ) Immunoblot for p66ShcS36 phosphorylation in human umbilical vein endothelial cells (HUVECs) overexpressing SUMO2 (Ad-HA-SUMO2) or p66ShcWT (Ad-Flag-p66ShcWT). ( D ) Quantification of p66ShcS36 phosphorylation from C. *p<0.05; n=8; one-way ANOVA. ( E ) Immunoblot for p66ShcS36 phosphorylation in HUVECs expressing SUMO2 (Ad-HA-SUMO2) and p66ShcWT (Ad-Flag-p66ShcWT) or p66ShcK81R (Ad-Flag-p66ShcK81R). ( F ) Quantification of p66ShcS36 phosphorylation from E. *p<0.05, **p<0.01; n=11; two-way ANOVA. Immunoblots were quantified with iBright image analysis software. Data represents mean ± SEM. Immunoblots are representative of at least three independent experiments. Figure 4—source data 1. PDF file containing original western blots for . Figure 4—source data 2. Original files for western blot analysis displayed in . Figure 4—source data 3. PDF file containing original western blots for . Figure 4—source data 4. Original files for western blot analysis displayed in . Figure 4—source data 5. PDF file containing original western blots for . Figure 4—source data 6. Original files for western blot analysis displayed in .

Journal: eLife

Article Title: p66Shc mediates SUMO2-induced endothelial dysfunction

doi: 10.7554/eLife.106344

Figure Lengend Snippet: ( A ) Immunoblot for p66ShcS36 phosphorylation in HEK-293 cells overexpressing SUMO2 (HA-SUMO2) and p66ShcWT (Flag-p66ShcWT). ( B ) Quantification of p66ShcS36 phosphorylation from A. *p<0.05; n=6; one-way ANOVA. ( C ) Immunoblot for p66ShcS36 phosphorylation in human umbilical vein endothelial cells (HUVECs) overexpressing SUMO2 (Ad-HA-SUMO2) or p66ShcWT (Ad-Flag-p66ShcWT). ( D ) Quantification of p66ShcS36 phosphorylation from C. *p<0.05; n=8; one-way ANOVA. ( E ) Immunoblot for p66ShcS36 phosphorylation in HUVECs expressing SUMO2 (Ad-HA-SUMO2) and p66ShcWT (Ad-Flag-p66ShcWT) or p66ShcK81R (Ad-Flag-p66ShcK81R). ( F ) Quantification of p66ShcS36 phosphorylation from E. *p<0.05, **p<0.01; n=11; two-way ANOVA. Immunoblots were quantified with iBright image analysis software. Data represents mean ± SEM. Immunoblots are representative of at least three independent experiments. Figure 4—source data 1. PDF file containing original western blots for . Figure 4—source data 2. Original files for western blot analysis displayed in . Figure 4—source data 3. PDF file containing original western blots for . Figure 4—source data 4. Original files for western blot analysis displayed in . Figure 4—source data 5. PDF file containing original western blots for . Figure 4—source data 6. Original files for western blot analysis displayed in .

Techniques: Western Blot, Phospho-proteomics, Expressing, Analysis, Software

$( A ) Immunoblot for p66ShcWT in whole-cell lysates or the mitochondrial fraction of human umbilical vein endothelial cells (HUVECs) ectopically expressing SUMO2 (Ad-HA-SUMO2) and p66ShcWT (Ad-Flag-p66ShcWT). ( B ) Quantification of the relative abundance of p66Shc in the mitochondria from A. **p<0.01, ****p<0.0001; n=5; one-way ANOVA. ( C ) Immunoblot for p66Shc in whole-cell lysates or the mitochondrial fraction of HUVECs ectopically expressing SUMO2 (Ad-HA-SUMO2) and p66ShcWT Ad-Flag-p66ShcWT or p66ShcK81R (Ad-Flag-p66ShcK81R). ( D ) Quantification of relative abundance of p66Shc in the mitochondria from C. *p<0.05, **p<0.01; n=7; two-way ANOVA. Data represents mean ± SEM. Immunoblots are representative of at least three independent experiments. Figure 5—source data 1. PDF file containing original western blots for . Figure 5—source data 2. PDF file containing original western blots for . Figure 5—source data 3. PDF file containing original western blots for . Figure 5—source data 4. Original files for western blot analysis displayed in .$

Journal: eLife

Article Title: p66Shc mediates SUMO2-induced endothelial dysfunction

doi: 10.7554/eLife.106344

Figure Lengend Snippet: ( A ) Immunoblot for p66ShcWT in whole-cell lysates or the mitochondrial fraction of human umbilical vein endothelial cells (HUVECs) ectopically expressing SUMO2 (Ad-HA-SUMO2) and p66ShcWT (Ad-Flag-p66ShcWT). ( B ) Quantification of the relative abundance of p66Shc in the mitochondria from A. **p<0.01, ****p<0.0001; n=5; one-way ANOVA. ( C ) Immunoblot for p66Shc in whole-cell lysates or the mitochondrial fraction of HUVECs ectopically expressing SUMO2 (Ad-HA-SUMO2) and p66ShcWT Ad-Flag-p66ShcWT or p66ShcK81R (Ad-Flag-p66ShcK81R). ( D ) Quantification of relative abundance of p66Shc in the mitochondria from C. *p<0.05, **p<0.01; n=7; two-way ANOVA. Data represents mean ± SEM. Immunoblots are representative of at least three independent experiments. Figure 5—source data 1. PDF file containing original western blots for . Figure 5—source data 2. PDF file containing original western blots for . Figure 5—source data 3. PDF file containing original western blots for . Figure 5—source data 4. Original files for western blot analysis displayed in .

Techniques: Western Blot, Expressing, Analysis

( A ) Immunoblot showing oxidized-LDL (o-LDL)-induced changes in small ubiquitin-like modifier 2/3 (SUMO2/3)-conjugated proteins in human umbilical vein endothelial cells (HUVECs) with and without SUMO2. ( B ) Graph showing serum cholesterol-level in wild-type, Ldlr -/- mice on a high fat diet, and Shc1 K81R mice on a high fat diet (n=4/group). One-way ANOVA, ****p<0.0001. Graphs showing % contraction following induction of endothelium-dependent relaxation ( C ) and endothelium-independent relaxation ( D ) in aortic rings from male wild-type mice, (n=14 rings from 5 mice), Ldlr -/- mice on a high fat diet (n=18 rings from 6 mice) and Ldlr - /- :: Shc1 K81R mice on a high fat diet (n=12 rings from 6 mice). Two-way ANOVA. ***p<0.001. ( E ) Representative images showing the level of 8-OHdG in aortic section of Ldlr -/- mice on a high fat diet (n=6 mice) and Ldlr -/- :: Shc1 K81R mice on a high fat diet (n=6 mice) and their ( F ) quantification. One-way ANOVA. *p<0.05. HFD, high fat diet; Ach, acetylcholine; SNP, sodium nitroprusside; PE, Phenylephrine; 8-OHdG, 8 hydroxy deoxyguanosine. Data represents mean ± SEM. Figure 7—source data 1. Source data for immunoblots in . Figure 7—source data 2. Original datasets for .

Journal: eLife

Article Title: p66Shc mediates SUMO2-induced endothelial dysfunction

doi: 10.7554/eLife.106344

Figure Lengend Snippet: ( A ) Immunoblot showing oxidized-LDL (o-LDL)-induced changes in small ubiquitin-like modifier 2/3 (SUMO2/3)-conjugated proteins in human umbilical vein endothelial cells (HUVECs) with and without SUMO2. ( B ) Graph showing serum cholesterol-level in wild-type, Ldlr -/- mice on a high fat diet, and Shc1 K81R mice on a high fat diet (n=4/group). One-way ANOVA, ****p<0.0001. Graphs showing % contraction following induction of endothelium-dependent relaxation ( C ) and endothelium-independent relaxation ( D ) in aortic rings from male wild-type mice, (n=14 rings from 5 mice), Ldlr -/- mice on a high fat diet (n=18 rings from 6 mice) and Ldlr - /- :: Shc1 K81R mice on a high fat diet (n=12 rings from 6 mice). Two-way ANOVA. ***p<0.001. ( E ) Representative images showing the level of 8-OHdG in aortic section of Ldlr -/- mice on a high fat diet (n=6 mice) and Ldlr -/- :: Shc1 K81R mice on a high fat diet (n=6 mice) and their ( F ) quantification. One-way ANOVA. *p<0.05. HFD, high fat diet; Ach, acetylcholine; SNP, sodium nitroprusside; PE, Phenylephrine; 8-OHdG, 8 hydroxy deoxyguanosine. Data represents mean ± SEM. Figure 7—source data 1. Source data for immunoblots in . Figure 7—source data 2. Original datasets for .

Techniques: Western Blot, Ubiquitin Proteomics

( A ) Representative immunoblots of endothelial cell lysate expressing Flag-p66ShcWT, Flag-p66ShcK81R, and HA-SUMO2 (n=3 shown, n=6 performed). ( B ) Bubble chart representation of all the regulatory pathways impacted by SUMO2-p66Shc in endothelial cells, rescued by p66ShcK81R. Pathway enrichment analysis was performed using R-based Reactome pathway analysis and visualization packages on differential protein expression data obtained from HUVECs expressing SUMO2-p66ShcWT or SUMO2-p66ShcK81R compared with Ad-LacZ controls (n=6). Figure 8—figure supplement 1—source data 1. PDF file containing original western blots for . Figure 8—figure supplement 1—source data 2. Original files for western blot analysis displayed in .

Journal: eLife

Article Title: p66Shc mediates SUMO2-induced endothelial dysfunction

doi: 10.7554/eLife.106344

Figure Lengend Snippet: ( A ) Representative immunoblots of endothelial cell lysate expressing Flag-p66ShcWT, Flag-p66ShcK81R, and HA-SUMO2 (n=3 shown, n=6 performed). ( B ) Bubble chart representation of all the regulatory pathways impacted by SUMO2-p66Shc in endothelial cells, rescued by p66ShcK81R. Pathway enrichment analysis was performed using R-based Reactome pathway analysis and visualization packages on differential protein expression data obtained from HUVECs expressing SUMO2-p66ShcWT or SUMO2-p66ShcK81R compared with Ad-LacZ controls (n=6). Figure 8—figure supplement 1—source data 1. PDF file containing original western blots for . Figure 8—figure supplement 1—source data 2. Original files for western blot analysis displayed in .

Techniques: Western Blot, Expressing, Analysis

( A, B ) Principal component analysis (PCA) of proteins expressed in endothelial cells expressing SUMO2+p66ShcWT and SUMO2+p66ShcK81R. ( C, D ) Bar chart presentation of key regulatory pathways differentially affected by small ubiquitin-like modifier 2 (SUMO2)-p66ShcWT ( C ) and SUMO2-p66ShcK81R ( D ) in endothelial cells. The protein expression data (fold change) were obtained from human umbilical vein endothelial cells (HUVECs) expressing SUMO2 with p66ShcWT or p66ShcK81R compared to those expressing Ad-Lac Z. Pathway enrichment analysis ( B ) was performed using R-based bioinformatics tools, including PROGENy/decoupleR pathway activity inference and visualization packages. Differentially regulated pathways associated with senescence, mitochondrial dysfunction, apoptosis signaling, and angiogenesis are shown. Data were derived from a sample size of n=6.

Journal: eLife

Article Title: p66Shc mediates SUMO2-induced endothelial dysfunction

doi: 10.7554/eLife.106344

Figure Lengend Snippet: ( A, B ) Principal component analysis (PCA) of proteins expressed in endothelial cells expressing SUMO2+p66ShcWT and SUMO2+p66ShcK81R. ( C, D ) Bar chart presentation of key regulatory pathways differentially affected by small ubiquitin-like modifier 2 (SUMO2)-p66ShcWT ( C ) and SUMO2-p66ShcK81R ( D ) in endothelial cells. The protein expression data (fold change) were obtained from human umbilical vein endothelial cells (HUVECs) expressing SUMO2 with p66ShcWT or p66ShcK81R compared to those expressing Ad-Lac Z. Pathway enrichment analysis ( B ) was performed using R-based bioinformatics tools, including PROGENy/decoupleR pathway activity inference and visualization packages. Differentially regulated pathways associated with senescence, mitochondrial dysfunction, apoptosis signaling, and angiogenesis are shown. Data were derived from a sample size of n=6.

Techniques: Analysis, Expressing, Ubiquitin Proteomics, Activity Assay, Derivative Assay

Amplex Red assay performed in HUVECs with and without knockdown of p66Shc expressing SUMO2 (Ad-SUMO2) or a control virus (Ad-LacZ).

Journal: eLife

Article Title: p66Shc mediates SUMO2-induced endothelial dysfunction

doi: 10.7554/eLife.106344

Figure Lengend Snippet: Amplex Red assay performed in HUVECs with and without knockdown of p66Shc expressing SUMO2 (Ad-SUMO2) or a control virus (Ad-LacZ).

Techniques: Amplex Red Assay, Knockdown, Expressing, Control, Virus

Cell viability graphs of studied cell lines (A: DLD‐1; B: HL‐60 and C: HUVEC cell lines).

Journal: Chemistry & Biodiversity

Article Title: Biological Activities and Phytochemical Composition of Arum rupicola var. rupicola : An In Vitro and In Silico Study

doi: 10.1002/cbdv.71785

Figure Lengend Snippet: Cell viability graphs of studied cell lines (A: DLD‐1; B: HL‐60 and C: HUVEC cell lines).

Article Snippet: Cytotoxicity studies were conducted using DLD‐1 (colon carcinoma; ATCC CVCL_0248), HUVEC (normal endothelial; ATCC CCVL_2959), and HL‐60 (leukemia; ATCC CVCL_0002) cell lines.

Techniques:

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human umbilical vein endothelial cells huvecs (PromoCell)

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