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huprot microarray screen  (StressMarq)


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    Structured Review

    StressMarq huprot microarray screen
    Proteome and peptide screens were used to evaluate potential off-target binding of affinity-purified p4796kb antibodies. a Manhattan plot displays results from the HuProt Human Proteome <t>Microarray</t> screen of antibody binding. Colors represent each block from the array, containing approximately 1000 proteins each. Spots represent individual protein intensity score from array. Hits are annotated with protein names. b Manhattan plot from peptide counter screen of 21 putative hits from proteome screen. Overlapping 15mer peptides were synthesized and printed on arrays. Spots represent individual peptide intensity scores from array. Peptide sequences of hits are displayed. Sera from guinea pigs immunized with p4796kb were tested for binding to putative hits from screens ( c ) and calcitonin family ( d ) measured by ELISA. Pre-immune sera were used as controls. Data are presented as means +/− SEM; n = 3. *** p < 0.0001.
    Huprot Microarray Screen, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/huprot+microarray+screen/HSP90+beta+Protein/pmc12041250-176-9-15
    Average 93 stars, based on 18 article reviews
    huprot microarray screen - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Preclinical characterization of an active immunotherapy targeting calcitonin gene-related peptide"

    Article Title: Preclinical characterization of an active immunotherapy targeting calcitonin gene-related peptide

    Journal: Communications Medicine

    doi: 10.1038/s43856-025-00870-2

    Proteome and peptide screens were used to evaluate potential off-target binding of affinity-purified p4796kb antibodies. a Manhattan plot displays results from the HuProt Human Proteome Microarray screen of antibody binding. Colors represent each block from the array, containing approximately 1000 proteins each. Spots represent individual protein intensity score from array. Hits are annotated with protein names. b Manhattan plot from peptide counter screen of 21 putative hits from proteome screen. Overlapping 15mer peptides were synthesized and printed on arrays. Spots represent individual peptide intensity scores from array. Peptide sequences of hits are displayed. Sera from guinea pigs immunized with p4796kb were tested for binding to putative hits from screens ( c ) and calcitonin family ( d ) measured by ELISA. Pre-immune sera were used as controls. Data are presented as means +/− SEM; n = 3. *** p < 0.0001.
    Figure Legend Snippet: Proteome and peptide screens were used to evaluate potential off-target binding of affinity-purified p4796kb antibodies. a Manhattan plot displays results from the HuProt Human Proteome Microarray screen of antibody binding. Colors represent each block from the array, containing approximately 1000 proteins each. Spots represent individual protein intensity score from array. Hits are annotated with protein names. b Manhattan plot from peptide counter screen of 21 putative hits from proteome screen. Overlapping 15mer peptides were synthesized and printed on arrays. Spots represent individual peptide intensity scores from array. Peptide sequences of hits are displayed. Sera from guinea pigs immunized with p4796kb were tested for binding to putative hits from screens ( c ) and calcitonin family ( d ) measured by ELISA. Pre-immune sera were used as controls. Data are presented as means +/− SEM; n = 3. *** p < 0.0001.

    Techniques Used: Binding Assay, Affinity Purification, Microarray, Blocking Assay, Synthesized, Enzyme-linked Immunosorbent Assay

    Related Articles

    Incubation:

    Article Title: Hsp90 Binds Directly to Fibronectin (FN) and Inhibition Reduces the Extracellular Fibronectin Matrix in Breast Cancer Cells
    Article Snippet: .. Hsp90β antibody or BSA bound beads were incubated with 1 μg Hsp90β (SPR-102A/B/C, StressMarq, USA) and/or soluble FN (sc-29011, Santa Cruz Biotechnology, USA) for 15 minutes, washed and immunoprecipitates eluted. ..

    Article Title: Heat Shock protein 90: Role in Enterovirus 71 Entry and Assembly and Potential Target for Therapy
    Article Snippet: .. 10 4 pfu of EV71 were mixed with 1 μg of recombinant HSP90β (Cat. No. SPR-102, StressMarq Biosciences Inc., Canada) and incubated at 4°C for 5 hours with occasionally rocking. .. Following HSP90β-viruses mixtures was rocked with 5 μg of anti-EV71 E1 antibody or non-immune mouse IgG at 4°C for overnight prior to be immunoprecipitated by adding protein G agarose beads (Santa Cruz Biotechnology) for another 3 hours.

    SPR Assay:

    Article Title: Hsp90 Binds Directly to Fibronectin (FN) and Inhibition Reduces the Extracellular Fibronectin Matrix in Breast Cancer Cells
    Article Snippet: .. Hsp90β antibody or BSA bound beads were incubated with 1 μg Hsp90β (SPR-102A/B/C, StressMarq, USA) and/or soluble FN (sc-29011, Santa Cruz Biotechnology, USA) for 15 minutes, washed and immunoprecipitates eluted. ..

    Article Title: Heat Shock protein 90: Role in Enterovirus 71 Entry and Assembly and Potential Target for Therapy
    Article Snippet: .. 10 4 pfu of EV71 were mixed with 1 μg of recombinant HSP90β (Cat. No. SPR-102, StressMarq Biosciences Inc., Canada) and incubated at 4°C for 5 hours with occasionally rocking. .. Following HSP90β-viruses mixtures was rocked with 5 μg of anti-EV71 E1 antibody or non-immune mouse IgG at 4°C for overnight prior to be immunoprecipitated by adding protein G agarose beads (Santa Cruz Biotechnology) for another 3 hours.

    Article Title: Heat Shock protein 90: Role in Enterovirus 71 Entry and Assembly and Potential Target for Therapy
    Article Snippet: Geldanamycin (GA; Cat. No. ant-gl) and 17-allyamino-17-demethoxygeldanamycin (17-AAG; Cat. No. ant-agl) were purchased from InvivoGen, CA, USA MG132 (Cat. No. c2211) was purchased from Sigma-Aldrich, MO, USA. .. Recombinant HSP90β (Cat. No. SPR-102) was obtained from Stress Marq Biosciences Inc., British Columbia, Canada. .. Monoclonal antibody, Mab979 recognized VP0/VP2 capsid protein of EV71 [ ] was purchased from Millipore, Inc., MA, USA A VP1-specific monoclonal antibody E1 produced in house had been described [ ].

    other:

    Article Title: Preclinical characterization of an active immunotherapy targeting calcitonin gene-related peptide.
    Article Snippet: Binding of p4796kb-derived sera to 3 hits from the HuProtTM microarray screen (HSP90 beta protein, StressMarq Bioscience, SPR-102B; AKR1B10 protein, MyBioSource, MBS203315; PTPRD protein, Acro, PTD-H52H9) and to other propeptides that belong to the calcitonin/CGRP peptide family, including recombinant adrenomedullin (ADM, MyBioSource, MBS2012013), recombinant adrenomedullin 2 (ADM2, MyBioSource, MBS2123890), synthetic amylin (Abcam, ab142398), and recombinant calcitonin (Abcam, ab153793), were further evaluated.

    Article Title: Preclinical characterization of an active immunotherapy targeting calcitonin gene-related peptide
    Article Snippet: Binding of p4796kb-derived sera to 3 hits from the HuProtTM microarray screen (HSP90 beta protein, StressMarq Bioscience, SPR-102B; AKR1B10 protein, MyBioSource, MBS203315; PTPRD protein, Acro, PTD-H52H9) and to other propeptides that belong to the calcitonin/CGRP peptide family, including recombinant adrenomedullin (ADM, MyBioSource, MBS2012013), recombinant adrenomedullin 2 (ADM2, MyBioSource, MBS2123890), synthetic amylin (Abcam, ab142398), and recombinant calcitonin (Abcam, ab153793), were further evaluated.

    Recombinant:

    Article Title: Heat Shock protein 90: Role in Enterovirus 71 Entry and Assembly and Potential Target for Therapy
    Article Snippet: .. 10 4 pfu of EV71 were mixed with 1 μg of recombinant HSP90β (Cat. No. SPR-102, StressMarq Biosciences Inc., Canada) and incubated at 4°C for 5 hours with occasionally rocking. .. Following HSP90β-viruses mixtures was rocked with 5 μg of anti-EV71 E1 antibody or non-immune mouse IgG at 4°C for overnight prior to be immunoprecipitated by adding protein G agarose beads (Santa Cruz Biotechnology) for another 3 hours.

    Article Title: Heat Shock protein 90: Role in Enterovirus 71 Entry and Assembly and Potential Target for Therapy
    Article Snippet: Geldanamycin (GA; Cat. No. ant-gl) and 17-allyamino-17-demethoxygeldanamycin (17-AAG; Cat. No. ant-agl) were purchased from InvivoGen, CA, USA MG132 (Cat. No. c2211) was purchased from Sigma-Aldrich, MO, USA. .. Recombinant HSP90β (Cat. No. SPR-102) was obtained from Stress Marq Biosciences Inc., British Columbia, Canada. .. Monoclonal antibody, Mab979 recognized VP0/VP2 capsid protein of EV71 [ ] was purchased from Millipore, Inc., MA, USA A VP1-specific monoclonal antibody E1 produced in house had been described [ ].

    Knockdown:

    Article Title: Hsp90 Binds Directly to Fibronectin (FN) and Inhibition Reduces the Extracellular Fibronectin Matrix in Breast Cancer Cells
    Article Snippet: Knockdown of Hsp90α or Hsp90β was performed using isoform specific siRNA (Hsp90α: M-005187-02, Hsp90β: M-005186-02, Dharmacon, USA) or a scrambled, non-targeting pool of siRNA oligonucleotides (D-001206-13-05, Dharmacon, USA) to serve as a negative control for any observed knockdown. .. Levels of Hsp90 knockdown were analyzed by immunoblotting using mouse anti human Hsp90α (SMC-147A/B, StressMarq, USA) and mouse anti human Hsp90β (SMC-107A/B, StressMarq, USA) primary antibodies. ..

    Western Blot:

    Article Title: Hsp90 Binds Directly to Fibronectin (FN) and Inhibition Reduces the Extracellular Fibronectin Matrix in Breast Cancer Cells
    Article Snippet: Knockdown of Hsp90α or Hsp90β was performed using isoform specific siRNA (Hsp90α: M-005187-02, Hsp90β: M-005186-02, Dharmacon, USA) or a scrambled, non-targeting pool of siRNA oligonucleotides (D-001206-13-05, Dharmacon, USA) to serve as a negative control for any observed knockdown. .. Levels of Hsp90 knockdown were analyzed by immunoblotting using mouse anti human Hsp90α (SMC-147A/B, StressMarq, USA) and mouse anti human Hsp90β (SMC-107A/B, StressMarq, USA) primary antibodies. ..



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    StressMarq huprot microarray screen
    Proteome and peptide screens were used to evaluate potential off-target binding of affinity-purified p4796kb antibodies. a Manhattan plot displays results from the HuProt Human Proteome <t>Microarray</t> screen of antibody binding. Colors represent each block from the array, containing approximately 1000 proteins each. Spots represent individual protein intensity score from array. Hits are annotated with protein names. b Manhattan plot from peptide counter screen of 21 putative hits from proteome screen. Overlapping 15mer peptides were synthesized and printed on arrays. Spots represent individual peptide intensity scores from array. Peptide sequences of hits are displayed. Sera from guinea pigs immunized with p4796kb were tested for binding to putative hits from screens ( c ) and calcitonin family ( d ) measured by ELISA. Pre-immune sera were used as controls. Data are presented as means +/− SEM; n = 3. *** p < 0.0001.
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    hsp90 beta protein  (StressMarq)
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    Proteome and peptide screens were used to evaluate potential off-target binding of affinity-purified p4796kb antibodies. a Manhattan plot displays results from the HuProt Human Proteome <t>Microarray</t> screen of antibody binding. Colors represent each block from the array, containing approximately 1000 proteins each. Spots represent individual protein intensity score from array. Hits are annotated with protein names. b Manhattan plot from peptide counter screen of 21 putative hits from proteome screen. Overlapping 15mer peptides were synthesized and printed on arrays. Spots represent individual peptide intensity scores from array. Peptide sequences of hits are displayed. Sera from guinea pigs immunized with p4796kb were tested for binding to putative hits from screens ( c ) and calcitonin family ( d ) measured by ELISA. Pre-immune sera were used as controls. Data are presented as means +/− SEM; n = 3. *** p < 0.0001.
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    Image Search Results


    Proteome and peptide screens were used to evaluate potential off-target binding of affinity-purified p4796kb antibodies. a Manhattan plot displays results from the HuProt Human Proteome Microarray screen of antibody binding. Colors represent each block from the array, containing approximately 1000 proteins each. Spots represent individual protein intensity score from array. Hits are annotated with protein names. b Manhattan plot from peptide counter screen of 21 putative hits from proteome screen. Overlapping 15mer peptides were synthesized and printed on arrays. Spots represent individual peptide intensity scores from array. Peptide sequences of hits are displayed. Sera from guinea pigs immunized with p4796kb were tested for binding to putative hits from screens ( c ) and calcitonin family ( d ) measured by ELISA. Pre-immune sera were used as controls. Data are presented as means +/− SEM; n = 3. *** p < 0.0001.

    Journal: Communications Medicine

    Article Title: Preclinical characterization of an active immunotherapy targeting calcitonin gene-related peptide

    doi: 10.1038/s43856-025-00870-2

    Figure Lengend Snippet: Proteome and peptide screens were used to evaluate potential off-target binding of affinity-purified p4796kb antibodies. a Manhattan plot displays results from the HuProt Human Proteome Microarray screen of antibody binding. Colors represent each block from the array, containing approximately 1000 proteins each. Spots represent individual protein intensity score from array. Hits are annotated with protein names. b Manhattan plot from peptide counter screen of 21 putative hits from proteome screen. Overlapping 15mer peptides were synthesized and printed on arrays. Spots represent individual peptide intensity scores from array. Peptide sequences of hits are displayed. Sera from guinea pigs immunized with p4796kb were tested for binding to putative hits from screens ( c ) and calcitonin family ( d ) measured by ELISA. Pre-immune sera were used as controls. Data are presented as means +/− SEM; n = 3. *** p < 0.0001.

    Article Snippet: Binding of p4796kb-derived sera to 3 hits from the HuProt™ microarray screen (HSP90 beta protein, StressMarq Bioscience, SPR-102B; AKR1B10 protein, MyBioSource, MBS203315; PTPRD protein, Acro, PTD-H52H9) and to other propeptides that belong to the calcitonin/CGRP peptide family, including recombinant adrenomedullin (ADM, MyBioSource, MBS2012013), recombinant adrenomedullin 2 (ADM2, MyBioSource, MBS2123890), synthetic amylin (Abcam, ab142398), and recombinant calcitonin (Abcam, ab153793), were further evaluated.

    Techniques: Binding Assay, Affinity Purification, Microarray, Blocking Assay, Synthesized, Enzyme-linked Immunosorbent Assay