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CapitalBio Corporation cdna microarray analyses
Cdna Microarray Analyses, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+whole+genome+microarray+chip/cdna+microarray+analysis/pmc04747391-146-14-19
Average 90 stars, based on 1 article reviews
cdna microarray analyses - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Microarray:

Article Title: Matrine-induced autophagy regulated by p53 through AMP-activated protein kinase in human hepatoma cells.
Article Snippet: Total RNA was extracted from matrine-treated HepG2 cells using the TRIzol reagents (Invitrogen). .. The cDNA microarray analysis was performed by CapitalBio Co. (Peking, China) according to the standardized protocol. .. A Lux-Scan 10KA dual pathways laser scanner (CapitalBio) and a GenePix Pro 4.0 image analysis software (Axon Instruments, Inc., Union City, CA, USA) was applied to scan the chips and analyze the images, respectively.

Article Title: MicroRNA-124 reduces the pentose phosphate pathway and proliferation by targeting PRPS1 and RPIA mRNAs in human colorectal cancer cells.
Article Snippet: This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Epigenetic downregulation of the ISG15–conjugating enzyme UbcH8 impairs lipolysis and correlates with poor prognosis in nasopharyngeal carcinoma
Article Snippet: .. Total RNA from NPC cells was isolated using Trizol reagent (Invitrogen), and subjected to human cDNA microarray analyses by CapitalBio CO. (Beijing, China). ..

Article Title: Loss of RPS41 but not its paralog RPS42 results in altered growth, filamentation and transcriptome changes in Candida albicans.
Article Snippet: 31 32 33 34 35 36 37 38 39 40 41 42 Article history: Received 7 November 2014 Revised 23 March 2015 Accepted 31 March 2015 Available online xxxx

Article Title: Jasmonic acid and ethylene signaling pathways participate in the defense response of Chinese cabbage to Pectobacterium carotovorum infection
Article Snippet: .. The cDNA microarray hybridization was performed at CapitalBio Co. Ltd. (Beijing, China). .. Hybridization images were obtained using a ScanArray Express scanner (Packard Bioscience, Meriden, CT, USA) and analyzed with GenePix Pro 4.0 (Axon Instruments, USA).

Transfection:

Article Title: MicroRNA-124 reduces the pentose phosphate pathway and proliferation by targeting PRPS1 and RPIA mRNAs in human colorectal cancer cells.
Article Snippet: This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Hybridization:

Article Title: Profiling Ethylene-Responsive Genes Expressed in the Latex of the Mature Virgin Rubber Trees Using cDNA Microarray
Article Snippet: .. Probe hybridization with the generated latex cDNA microarrays was performed in a BioMixer TM II hybridization station (CapitalBio, Beijing, China) as previously described [ , ]. ..

Article Title: Long non-coding RNAs as targets for immunosuppressive drug teriflunomide in anti-cancer potential for hepatocellular carcinoma.
Article Snippet: Hepatocellular carcinoma (HCC) is the most common form of liver cancer.. Because of the relatively chemotherapy-refractory nature of HCC and significant potential poor hepatic reserve, chemotherapy has not been used consistently in the treatment of HCC.. Effective new drugs for HCC are urgently needed.

Article Title: Jasmonic acid and ethylene signaling pathways participate in the defense response of Chinese cabbage to Pectobacterium carotovorum infection
Article Snippet: .. The cDNA microarray hybridization was performed at CapitalBio Co. Ltd. (Beijing, China). .. Hybridization images were obtained using a ScanArray Express scanner (Packard Bioscience, Meriden, CT, USA) and analyzed with GenePix Pro 4.0 (Axon Instruments, USA).

Generated:

Article Title: Profiling Ethylene-Responsive Genes Expressed in the Latex of the Mature Virgin Rubber Trees Using cDNA Microarray
Article Snippet: .. Probe hybridization with the generated latex cDNA microarrays was performed in a BioMixer TM II hybridization station (CapitalBio, Beijing, China) as previously described [ , ]. ..

Isolation:

Article Title: Epigenetic downregulation of the ISG15–conjugating enzyme UbcH8 impairs lipolysis and correlates with poor prognosis in nasopharyngeal carcinoma
Article Snippet: .. Total RNA from NPC cells was isolated using Trizol reagent (Invitrogen), and subjected to human cDNA microarray analyses by CapitalBio CO. (Beijing, China). ..

Labeling:

Article Title: Long non-coding RNAs as targets for immunosuppressive drug teriflunomide in anti-cancer potential for hepatocellular carcinoma.
Article Snippet: Hepatocellular carcinoma (HCC) is the most common form of liver cancer.. Because of the relatively chemotherapy-refractory nature of HCC and significant potential poor hepatic reserve, chemotherapy has not been used consistently in the treatment of HCC.. Effective new drugs for HCC are urgently needed.



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Image Search Results


Scatter plot of the gene differential expression in PC-2 cells induced by RPM. Notes: The Cy3 (RPM treated group) and Cy5 (untreated control) channel intensities from the two-color DNA microarray experiments were shown in the scatter plot. The variables appear in a linear relationship, and the linear correlation between them is 0.935. The red plots represented upregulated genes, and the green plots represented downregulated genes. Abbreviation: RPM, rapamycin.

Journal: Drug Design, Development and Therapy

Article Title: Targeted inhibition of mammalian target of rapamycin (mTOR) enhances radiosensitivity in pancreatic carcinoma cells

doi: 10.2147/DDDT.S42390

Figure Lengend Snippet: Scatter plot of the gene differential expression in PC-2 cells induced by RPM. Notes: The Cy3 (RPM treated group) and Cy5 (untreated control) channel intensities from the two-color DNA microarray experiments were shown in the scatter plot. The variables appear in a linear relationship, and the linear correlation between them is 0.935. The red plots represented upregulated genes, and the green plots represented downregulated genes. Abbreviation: RPM, rapamycin.

Article Snippet: We used an Agilent Whole Human Genome Microarray chip to assess the expression profiles in RPM-treated cells.

Techniques: Expressing, Microarray

( A ) RT-PCR analysis for the DNA-damage repair and transcription genes after the treatment with RPM; ( B ) Difference between the cDNA microarray and RT-PCR in DNA-damage repair and transcription genes. Notes: The genes examined here are: (1) DDB1(NM_001923); (2) RAD51 (NM_002876); (3) XRCC5 (NM_021141); (4) PCNA (NM_002592); and (5) ABCC4 (NM_005845). The values represent the mean ± SD of the data from three independent experiments. Abbreviations: RPM, rapamycin; RT-PCR, reverse transcription polymerase chain reaction; SD, standard deviation.

Journal: Drug Design, Development and Therapy

Article Title: Targeted inhibition of mammalian target of rapamycin (mTOR) enhances radiosensitivity in pancreatic carcinoma cells

doi: 10.2147/DDDT.S42390

Figure Lengend Snippet: ( A ) RT-PCR analysis for the DNA-damage repair and transcription genes after the treatment with RPM; ( B ) Difference between the cDNA microarray and RT-PCR in DNA-damage repair and transcription genes. Notes: The genes examined here are: (1) DDB1(NM_001923); (2) RAD51 (NM_002876); (3) XRCC5 (NM_021141); (4) PCNA (NM_002592); and (5) ABCC4 (NM_005845). The values represent the mean ± SD of the data from three independent experiments. Abbreviations: RPM, rapamycin; RT-PCR, reverse transcription polymerase chain reaction; SD, standard deviation.

Article Snippet: We used an Agilent Whole Human Genome Microarray chip to assess the expression profiles in RPM-treated cells.

Techniques: Reverse Transcription Polymerase Chain Reaction, Microarray, Standard Deviation

Characteristics of the samples used for  microarray  analysis

Journal: Arthritis Research & Therapy

Article Title: Genome-wide expression profiles of subchondral bone in osteoarthritis

doi: 10.1186/ar4380

Figure Lengend Snippet: Characteristics of the samples used for microarray analysis

Article Snippet: Cyanine 3-labeled cRNA was then purified and hybridized to Agilent whole human genome 44 k microarray chips (Agilent Technologies).

Techniques: Microarray

Microarray analyses of gene expression of osteoarthritis and non-osteoarthritis of subchondral bone and qRT-PCR validation in osteoarthritis knee subchondral bone. (A) Differentially expressed genes were classified based on their expression levels with a minimum of twofold, threefold, fourfold, sixfold, and eightfold changes. (B) Unsupervised hierarchical clustering of osteoarthritis (OA) and non-OA samples was performed for genes whose differential expression exceeded twofold (972 genes). Distances between samples were detected with a Euclidean algorithm and clustered with a centroid linkage method. The OA medial tibial (MT) samples (gray) were clustered together and clearly separated from the OA lateral tibial (LT) (brown) and non-OA samples. Non-OA LT samples (N-LT, red) and non-OA MT samples (N-MT, blue) were clustered together with OA LT samples. (C) Eighty-five genes (plus GAPDH ) were selected for quantitative reverse-transcription polymerase chain reaction (qRT-PCR) validation on a separate group of nine OA subchondral bone specimens. The results of qRT-PCR were strongly consistent with those of microarray analysis ( R 2 = 0.5764, P <0.0001). FC, fold-change.

Journal: Arthritis Research & Therapy

Article Title: Genome-wide expression profiles of subchondral bone in osteoarthritis

doi: 10.1186/ar4380

Figure Lengend Snippet: Microarray analyses of gene expression of osteoarthritis and non-osteoarthritis of subchondral bone and qRT-PCR validation in osteoarthritis knee subchondral bone. (A) Differentially expressed genes were classified based on their expression levels with a minimum of twofold, threefold, fourfold, sixfold, and eightfold changes. (B) Unsupervised hierarchical clustering of osteoarthritis (OA) and non-OA samples was performed for genes whose differential expression exceeded twofold (972 genes). Distances between samples were detected with a Euclidean algorithm and clustered with a centroid linkage method. The OA medial tibial (MT) samples (gray) were clustered together and clearly separated from the OA lateral tibial (LT) (brown) and non-OA samples. Non-OA LT samples (N-LT, red) and non-OA MT samples (N-MT, blue) were clustered together with OA LT samples. (C) Eighty-five genes (plus GAPDH ) were selected for quantitative reverse-transcription polymerase chain reaction (qRT-PCR) validation on a separate group of nine OA subchondral bone specimens. The results of qRT-PCR were strongly consistent with those of microarray analysis ( R 2 = 0.5764, P <0.0001). FC, fold-change.

Article Snippet: Cyanine 3-labeled cRNA was then purified and hybridized to Agilent whole human genome 44 k microarray chips (Agilent Technologies).

Techniques: Microarray, Expressing, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction

Top ten upregulated and downregulated genes comparing lateral tibial bone (below intact cartilage) with medial tibial bone (below lesioned cartilage)

Journal: Arthritis Research & Therapy

Article Title: Genome-wide expression profiles of subchondral bone in osteoarthritis

doi: 10.1186/ar4380

Figure Lengend Snippet: Top ten upregulated and downregulated genes comparing lateral tibial bone (below intact cartilage) with medial tibial bone (below lesioned cartilage)

Article Snippet: Cyanine 3-labeled cRNA was then purified and hybridized to Agilent whole human genome 44 k microarray chips (Agilent Technologies).

Techniques: Microarray, Expressing, Binding Assay

Genes representative of gene clusters associated with osteoarthritis

Journal: Arthritis Research & Therapy

Article Title: Genome-wide expression profiles of subchondral bone in osteoarthritis

doi: 10.1186/ar4380

Figure Lengend Snippet: Genes representative of gene clusters associated with osteoarthritis

Article Snippet: Cyanine 3-labeled cRNA was then purified and hybridized to Agilent whole human genome 44 k microarray chips (Agilent Technologies).

Techniques: Microarray

Characteristics of KBD and control sample pairs

Journal: BMC Musculoskeletal Disorders

Article Title: Expression profile analysis of mycotoxin-related genes in cartilage with endemic osteochondropathy kashin-beck disease

doi: 10.1186/1471-2474-13-130

Figure Lengend Snippet: Characteristics of KBD and control sample pairs

Article Snippet: Total RNA was isolated from cartilage samples, following by being amplified, labeled and hybridized to Agilent human whole genome microarray chip. qRT-PCR was conducted to validate the microarray data.

Techniques: Microarray

Histogram showing the expression values of the selected 8 genes measured by microarray and qRT-PCR.

Journal: BMC Musculoskeletal Disorders

Article Title: Expression profile analysis of mycotoxin-related genes in cartilage with endemic osteochondropathy kashin-beck disease

doi: 10.1186/1471-2474-13-130

Figure Lengend Snippet: Histogram showing the expression values of the selected 8 genes measured by microarray and qRT-PCR.

Article Snippet: Total RNA was isolated from cartilage samples, following by being amplified, labeled and hybridized to Agilent human whole genome microarray chip. qRT-PCR was conducted to validate the microarray data.

Techniques: Expressing, Microarray, Quantitative RT-PCR