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paraffin embedded breast cancer tissue arrays  (Novus Biologicals)


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    Structured Review

    Novus Biologicals paraffin embedded breast cancer tissue arrays
    Paraffin Embedded Breast Cancer Tissue Arrays, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+tissue+microarray/Human+Breast+Tissue+MicroArray+(Cancer)/pm36608120-377-0-11
    Average 93 stars, based on 22 article reviews
    paraffin embedded breast cancer tissue arrays - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Immunohistochemistry:

    Article Title: FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells
    Article Snippet: After centrifugation, the chromatin IP and input were reverse-cross-linked by adding 5M NaCl (4 μL for input and 8 μL for IP sample) and incubated overnight at 65°C, and then DNA extracted using the Gel Extraction Kit (QIAGEN no. 28704). .. IHC analysis of normal breast and breast carcinoma specimens (Novus Biologicals no. NBP2–30212) were performed by antigen retrieval and blocking endogenous peroxidase activity using the BOND RX automated IHC system (Leica) followed by staining with anti-FBXO44 antibody (Sigma-Aldrich no. HPA003363) at 1:200 dilution. .. IHC staining was performed using the Bond Polymer Refine Detection Kit (Leica no. DS9800) and images scanned using Aperio ScanScope AT2 (Aperio Technologies).

    Blocking Assay:

    Article Title: FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells
    Article Snippet: After centrifugation, the chromatin IP and input were reverse-cross-linked by adding 5M NaCl (4 μL for input and 8 μL for IP sample) and incubated overnight at 65°C, and then DNA extracted using the Gel Extraction Kit (QIAGEN no. 28704). .. IHC analysis of normal breast and breast carcinoma specimens (Novus Biologicals no. NBP2–30212) were performed by antigen retrieval and blocking endogenous peroxidase activity using the BOND RX automated IHC system (Leica) followed by staining with anti-FBXO44 antibody (Sigma-Aldrich no. HPA003363) at 1:200 dilution. .. IHC staining was performed using the Bond Polymer Refine Detection Kit (Leica no. DS9800) and images scanned using Aperio ScanScope AT2 (Aperio Technologies).

    Activity Assay:

    Article Title: FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells
    Article Snippet: After centrifugation, the chromatin IP and input were reverse-cross-linked by adding 5M NaCl (4 μL for input and 8 μL for IP sample) and incubated overnight at 65°C, and then DNA extracted using the Gel Extraction Kit (QIAGEN no. 28704). .. IHC analysis of normal breast and breast carcinoma specimens (Novus Biologicals no. NBP2–30212) were performed by antigen retrieval and blocking endogenous peroxidase activity using the BOND RX automated IHC system (Leica) followed by staining with anti-FBXO44 antibody (Sigma-Aldrich no. HPA003363) at 1:200 dilution. .. IHC staining was performed using the Bond Polymer Refine Detection Kit (Leica no. DS9800) and images scanned using Aperio ScanScope AT2 (Aperio Technologies).

    Staining:

    Article Title: FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells
    Article Snippet: After centrifugation, the chromatin IP and input were reverse-cross-linked by adding 5M NaCl (4 μL for input and 8 μL for IP sample) and incubated overnight at 65°C, and then DNA extracted using the Gel Extraction Kit (QIAGEN no. 28704). .. IHC analysis of normal breast and breast carcinoma specimens (Novus Biologicals no. NBP2–30212) were performed by antigen retrieval and blocking endogenous peroxidase activity using the BOND RX automated IHC system (Leica) followed by staining with anti-FBXO44 antibody (Sigma-Aldrich no. HPA003363) at 1:200 dilution. .. IHC staining was performed using the Bond Polymer Refine Detection Kit (Leica no. DS9800) and images scanned using Aperio ScanScope AT2 (Aperio Technologies).

    In Vivo:

    Article Title: Unlocking the role of SH3PXD2B in epithelial-to-mesenchymal transition driving Breast Cancer Lung Metastasis
    Article Snippet: .. An in vivo tissue microarray study was performed on a Human Breast Tissue MicroArray (Cancer), NBP2-30212, that was procured from Novus Biologicals USA. ..

    Microarray:

    Article Title: Unlocking the role of SH3PXD2B in epithelial-to-mesenchymal transition driving Breast Cancer Lung Metastasis
    Article Snippet: .. An in vivo tissue microarray study was performed on a Human Breast Tissue MicroArray (Cancer), NBP2-30212, that was procured from Novus Biologicals USA. ..



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    Image Search Results


    Overexpression of DNPH1 in human breast tumors (A) DNPH1 mRNA expression in various breast cancer subtypes was analyzed in published microarray data. Mean with standard deviation and statistical significance compared to normal breast tissue are indicated; ∗∗∗∗, p < 0.0001 (one-way ANOVA, Tukey’s multiple comparisons test). (B) High (top quartile) DNPH1 mRNA levels predict reduced survival. Data derived from published microarray experiments ; p < 0.0001 (log rank test). (C) DNPH1 protein expression in human breast cell lines assayed by Western blotting. (D) Examples of immunohistochemical staining with DNPH1 antibodies on matching normal and cancerous breast tissue. Scale bars, 0.1 mm. (E) DNPH1 protein expression was measured by immunohistochemistry in 20 matching normal and cancerous human breast tissues; two-tailed, paired t test.

    Journal: iScience

    Article Title: Promotion of breast cancer by the DNPH1 enzyme

    doi: 10.1016/j.isci.2026.115227

    Figure Lengend Snippet: Overexpression of DNPH1 in human breast tumors (A) DNPH1 mRNA expression in various breast cancer subtypes was analyzed in published microarray data. Mean with standard deviation and statistical significance compared to normal breast tissue are indicated; ∗∗∗∗, p < 0.0001 (one-way ANOVA, Tukey’s multiple comparisons test). (B) High (top quartile) DNPH1 mRNA levels predict reduced survival. Data derived from published microarray experiments ; p < 0.0001 (log rank test). (C) DNPH1 protein expression in human breast cell lines assayed by Western blotting. (D) Examples of immunohistochemical staining with DNPH1 antibodies on matching normal and cancerous breast tissue. Scale bars, 0.1 mm. (E) DNPH1 protein expression was measured by immunohistochemistry in 20 matching normal and cancerous human breast tissues; two-tailed, paired t test.

    Article Snippet: Human breast tissue microarray , ISU Abxis , A312.

    Techniques: Over Expression, Expressing, Microarray, Standard Deviation, Derivative Assay, Western Blot, Immunohistochemical staining, Staining, Immunohistochemistry, Two Tailed Test

    Myxoid liposarcoma tissues present low levels of CD99 expression. A Representative image of tissue microarray staining of CD99 in Ewing’s sarcoma, liposarcoma myxoid, osteosarcoma, and chondrosarcoma taken with Objective 10 ×. B Quantification of CD99 signal in cancer tissue microarray. Each dot represents one patient-derived sample, where the signal was normalized to Ewing’s sarcoma signal, and then regrouped by cancer type. N = 2 independent experiments were averaged. Ewing’s sarcoma is represented by a dotted line at 100% and serves as a positive control for CD99 abundance level as previously reported

    Journal: BMC Biology

    Article Title: Proteomics analysis of human mesenchymal stromal/stem cell sarcomagenesis model identifies ALDH1A3 and CD99 as potential targets in the transformation process

    doi: 10.1186/s12915-025-02498-z

    Figure Lengend Snippet: Myxoid liposarcoma tissues present low levels of CD99 expression. A Representative image of tissue microarray staining of CD99 in Ewing’s sarcoma, liposarcoma myxoid, osteosarcoma, and chondrosarcoma taken with Objective 10 ×. B Quantification of CD99 signal in cancer tissue microarray. Each dot represents one patient-derived sample, where the signal was normalized to Ewing’s sarcoma signal, and then regrouped by cancer type. N = 2 independent experiments were averaged. Ewing’s sarcoma is represented by a dotted line at 100% and serves as a positive control for CD99 abundance level as previously reported

    Article Snippet: The tissue microarray (#NBP2-30,330) was purchased from Novus Biologicals (Toronto, ON, Canada).

    Techniques: Expressing, Microarray, Staining, Derivative Assay, Positive Control