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brcaprog1 microarray slides  (Cooperative Human Tissue Network (CHTN)

 
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    Cooperative Human Tissue Network (CHTN brcaprog1 microarray slides
    HKDC1 expression involves with tumor growth and metastasis in vivo . (A,B) Tissue Microarray slides <t>(#CHTN</t> <t>BrCaProg1)</t> were obtained from CHTN (Cooperative Human Tissue Network), and the immunohistochemistry was performed using a primary rabbit antibody for either PGC1β or HKDC1 and a second anti-rabbit- FITC. Sixty cells from normal breast tissue (normal), primary tumor (tumor), and metastatic (metastasis) tissues were quantitated by Image J. (A) Protein quantitation, n = 3. *, P < 0.05, vs. Normal group; ¶, P < 0.05 vs. Tumor group. (B) Representative pictures for (A) . (C–H) The nude mice were injected with treated MCF7 cells through the tail vein for in vivo xenograft tumor development study, and the treated mice were sacrificed for further analysis. (C) The tumor tissues from the lung were isolated for mRNA analysis by qPCR, n = 4. *, P < 0.05, vs. CTL group. (D) Superoxide anion release from tumor tissues, n = 5, *, P < 0.05, vs. CTL group; #, P < 0.05, vs. shPGC1β group. (E–G) Mice were killed upon 20% weight loss, and the lungs were harvested for terminal analysis. The metastatic tumor nodules from the lungs were counted, and then the formalin-fixed, paraffin-embedded tumor tissue of the lung was sectioned to 4 mm thickness, and the histopathological analyses were performed with H&E staining. Images were taken using a Carl Zeiss MIRAX MIDI slide scanner, and the lung tumor spots were analyzed using a 3DHISTECH Pannoramic Viewer. (E) Tumor colony formation in lung, n = 9. *, P < 0.05, vs. CTL group; ¶, P < 0.05, vs. ↑PGC1β group; #, P < 0.05, vs. shPGC1β group. (F) Quantitated lung tumor spots, n = 5. *, P < 0.05, vs. CTL group; ¶, P < 0.05, vs. ↑PGC1β group; #, P < 0.05, vs. shPGC1β group. (G) Representative picture by H&E staining. (H) Kaplan-Meier analysis comparing survival of mice between each treatment group, P -value represents log-rank Mantel-Cox test result, n = 9. Results are expressed as mean ± SEM.
    Brcaprog1 Microarray Slides, supplied by Cooperative Human Tissue Network (CHTN, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+tissue+microarray+slides/brcaprog1+microarray+slides/pmc06478765-146-0-0
    Average 90 stars, based on 1 article reviews
    brcaprog1 microarray slides - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "PGC1β Regulates Breast Tumor Growth and Metastasis by SREBP1-Mediated HKDC1 Expression"

    Article Title: PGC1β Regulates Breast Tumor Growth and Metastasis by SREBP1-Mediated HKDC1 Expression

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2019.00290

    HKDC1 expression involves with tumor growth and metastasis in vivo . (A,B) Tissue Microarray slides (#CHTN BrCaProg1) were obtained from CHTN (Cooperative Human Tissue Network), and the immunohistochemistry was performed using a primary rabbit antibody for either PGC1β or HKDC1 and a second anti-rabbit- FITC. Sixty cells from normal breast tissue (normal), primary tumor (tumor), and metastatic (metastasis) tissues were quantitated by Image J. (A) Protein quantitation, n = 3. *, P < 0.05, vs. Normal group; ¶, P < 0.05 vs. Tumor group. (B) Representative pictures for (A) . (C–H) The nude mice were injected with treated MCF7 cells through the tail vein for in vivo xenograft tumor development study, and the treated mice were sacrificed for further analysis. (C) The tumor tissues from the lung were isolated for mRNA analysis by qPCR, n = 4. *, P < 0.05, vs. CTL group. (D) Superoxide anion release from tumor tissues, n = 5, *, P < 0.05, vs. CTL group; #, P < 0.05, vs. shPGC1β group. (E–G) Mice were killed upon 20% weight loss, and the lungs were harvested for terminal analysis. The metastatic tumor nodules from the lungs were counted, and then the formalin-fixed, paraffin-embedded tumor tissue of the lung was sectioned to 4 mm thickness, and the histopathological analyses were performed with H&E staining. Images were taken using a Carl Zeiss MIRAX MIDI slide scanner, and the lung tumor spots were analyzed using a 3DHISTECH Pannoramic Viewer. (E) Tumor colony formation in lung, n = 9. *, P < 0.05, vs. CTL group; ¶, P < 0.05, vs. ↑PGC1β group; #, P < 0.05, vs. shPGC1β group. (F) Quantitated lung tumor spots, n = 5. *, P < 0.05, vs. CTL group; ¶, P < 0.05, vs. ↑PGC1β group; #, P < 0.05, vs. shPGC1β group. (G) Representative picture by H&E staining. (H) Kaplan-Meier analysis comparing survival of mice between each treatment group, P -value represents log-rank Mantel-Cox test result, n = 9. Results are expressed as mean ± SEM.
    Figure Legend Snippet: HKDC1 expression involves with tumor growth and metastasis in vivo . (A,B) Tissue Microarray slides (#CHTN BrCaProg1) were obtained from CHTN (Cooperative Human Tissue Network), and the immunohistochemistry was performed using a primary rabbit antibody for either PGC1β or HKDC1 and a second anti-rabbit- FITC. Sixty cells from normal breast tissue (normal), primary tumor (tumor), and metastatic (metastasis) tissues were quantitated by Image J. (A) Protein quantitation, n = 3. *, P < 0.05, vs. Normal group; ¶, P < 0.05 vs. Tumor group. (B) Representative pictures for (A) . (C–H) The nude mice were injected with treated MCF7 cells through the tail vein for in vivo xenograft tumor development study, and the treated mice were sacrificed for further analysis. (C) The tumor tissues from the lung were isolated for mRNA analysis by qPCR, n = 4. *, P < 0.05, vs. CTL group. (D) Superoxide anion release from tumor tissues, n = 5, *, P < 0.05, vs. CTL group; #, P < 0.05, vs. shPGC1β group. (E–G) Mice were killed upon 20% weight loss, and the lungs were harvested for terminal analysis. The metastatic tumor nodules from the lungs were counted, and then the formalin-fixed, paraffin-embedded tumor tissue of the lung was sectioned to 4 mm thickness, and the histopathological analyses were performed with H&E staining. Images were taken using a Carl Zeiss MIRAX MIDI slide scanner, and the lung tumor spots were analyzed using a 3DHISTECH Pannoramic Viewer. (E) Tumor colony formation in lung, n = 9. *, P < 0.05, vs. CTL group; ¶, P < 0.05, vs. ↑PGC1β group; #, P < 0.05, vs. shPGC1β group. (F) Quantitated lung tumor spots, n = 5. *, P < 0.05, vs. CTL group; ¶, P < 0.05, vs. ↑PGC1β group; #, P < 0.05, vs. shPGC1β group. (G) Representative picture by H&E staining. (H) Kaplan-Meier analysis comparing survival of mice between each treatment group, P -value represents log-rank Mantel-Cox test result, n = 9. Results are expressed as mean ± SEM.

    Techniques Used: Expressing, In Vivo, Microarray, Immunohistochemistry, Protein Quantitation, Injection, Isolation, Formalin-fixed Paraffin-Embedded, Staining

    Related Articles

    Microarray:

    Article Title: PGC1β Regulates Breast Tumor Growth and Metastasis by SREBP1-Mediated HKDC1 Expression
    Article Snippet: .. CHTN BrCaProg1 Microarray slides were obtained from CHTN, including normal, tumor, and metastatic breast cancer tissues. ..



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    Article Snippet: Human tumor tissue and healthy tissue microarray slides were obtained from Novus Biologicals (NBP2-30234, NBP2-78082, NBP2-30232, NBP2-30233, NBP2-30189).

    Techniques: Flow Cytometry, Quantitation Assay, Two Tailed Test, Cytotoxicity Assay, Cell Culture, Derivative Assay, Immunohistochemistry, Microarray, Staining, Immunohistochemical staining