ht29 (ATCC)
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ht29
Ht29, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2950 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+tissue+expression+pattern/HT-29/pmc06359839-49-8-37
Average 99 stars, based on 2950 article reviews
Ht29, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2950 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+tissue+expression+pattern/HT-29/pmc06359839-49-8-37
Average 99 stars, based on 2950 article reviews
ht29 - by Bioz Stars,
2026-11
99/100 stars
Images
1) Product Images from "Growth inhibition and apoptosis in colorectal cancer cells induced by Vitamin D-Nanoemulsion (NVD): involvement of Wnt/β-catenin and other signal transduction pathways"
Article Title: Growth inhibition and apoptosis in colorectal cancer cells induced by Vitamin D-Nanoemulsion (NVD): involvement of Wnt/β-catenin and other signal transduction pathways
Journal: Cell & Bioscience
doi: 10.1186/s13578-019-0277-z
Figure Legend Snippet: Inhibition of cologenecity in colorectal cancer cells by NVD. (Clonogenic assay; 7 days). a , b NVD administration (40 and 60 μM) of HCT116 and HT29 cells inhibiting colony formation. Each value represents a mean ± SD (n = 3)
Techniques Used: Inhibition, Clonogenic Assay
Figure Legend Snippet: a , b NVD treatment on HCT116 and HT29 cells resulted in accumulation of cells at the G2-phase and growth inhibition and apoptosis. After 24 h incubation of NVD treated cells and staining with propidium iodide, DNA content was analyzed by flow cytometry. c , d NVD treatment on HCT116 and HT29 cells resulted in accumulation of cells at the G2-phase. After 24 h incubation of NVD treated cells and staining with propidium iodide, DNA content was analyzed by flow cytometry. Percentage of cell population in G2-phase of the cell cycle is shown. Experiments were performed in triplicate
Techniques Used: Inhibition, Incubation, Staining, Flow Cytometry
Figure Legend Snippet: Percentage of cell population after administration of NVD
Techniques Used:
Figure Legend Snippet: Effect of NVD treatment of HCT116 and HT29 ( a , b ) cells on protein expression of cdc2, WAF1/p21, cdk 2, 4 cyclin A, B1, E2 and ( c , d ) showing active Caspase 3, 7, 9 and PARP. Cells were administrated with NVD (20, 40 and 60 μM) for 24 h and 48 h and harvested. Total cell lysates were prepared and 40 μM proteins was subjected to SDS page followed by immunoblot analysis and chemiluminescence detection. Equal loading of protein was verified by stripping the Immunoblot and again probing it for Actin. The immunoblots shown here are representative of three individual experiments with similar results
Techniques Used: Expressing, SDS Page, Western Blot, Stripping Membranes
Figure Legend Snippet: a Effect of treatment of NVD to HCT116 and HT29 cell lines on protein expression of Bax, Bcl2, Bak and Bcl-X L . b Immunoblot analysis of β-catenin expression of HCT116 and HT29 in NVD treated group as compared to control group. The cells were administrated with NVD for 48 h and harvested and cell lysates prepared. The data are representative of three independent experiments with similar results
Techniques Used: Expressing, Western Blot, Control
Figure Legend Snippet: a Modulation of β -catenin expression by NVD treatment in HCT116 ( a ) and HT29 ( b ) cells. mRNA expression of β-catenin in NVD treated HCT116 and HT29 cells (RT-PCR), experiment performed in triplicate (mean ± SD), **p < 0.01. b Effect of NVD on protein expression of β-catenin, Survivin and phosphorylation of Akt at Ser473 in HCT116 and HT29 cells., Total cell lysate were prepared and 40 μM proteins was subjected to SDS-page followed by Immunoblot analysis and chemiluminescence detection. Equal loading of protein was confirmed by stripping the immunoblot and reprobing it for β-Actin
Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Phospho-proteomics, SDS Page, Western Blot, Stripping Membranes
Figure Legend Snippet: Imunofluorescence staining of HCT116 ( b ) and HT29 ( d ) demonstrating expression of β-catenin in both NVD treated (60 µM) as compared to control HCT116 ( a ) and HT29 ( c ) cells (untreated). Alexa fluor staining of β-catenin of both cell lines (green fluorescence) and counter stained with DAPI (blue fluorescence) were observed
Techniques Used: Staining, Expressing, Control, Fluorescence
Figure Legend Snippet: Western blot analysis of cellular lysates prepared from HCT116 and HT29 cells. Lysates prepared from HCT116 and HT29 cells, initially seeded at density of 1.5 × 10 4 cells were administrated with 20, 40 and 60 µM concentrations of NVD. The blots probed with CK2 αα’, PI3-K, STAT3, STAT P-Try705, NFκB p65 P-S529, IκBα P-S32/36 and xIAP antibodies were shown. Actin blots are shown as loading controls. Data based on three different experiments, each carried out in triplicate
Techniques Used: Western Blot
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