Histopathology:Article Title: Reagents and methods for treating cancer and autoimmune disease
Article Snippet: Data analysis was done using FlowJoTM software (Ashland, Oreg.). .. Histopathology Human Multiple Normal and Tumor Tissue Arrays were purchased from BioChain (Newark, Calif.). .. The tissues were subjected to antigen unmasking, and then incubated with anti-TAPBPL monoclonal antibody, followed by ImmPRESSTM 4VR Polymer HRP anti-mouse IgG reagent, and developed with peroxidase substrate solution (Vector Laboratories) according to the manufacturer's instructions.
Immunohistochemistry:
Microarray:
Activity Assay:Article Title: Development of a recombinant hepatitis B immunoglobulin derived from B cells collected from healthy individuals administered with hepatitis B virus vaccines: A feasibility study.
Article Snippet: HBV infection was monitored by quantifying the HBV e antigen (HBeAg) in the culture medium with a commercial ELISA (UickTiterTM Hepatitis B “e” Antigen ELISA Kit, CELL BIOLABS, CA). .. Paraffin arrays of HBV human liver cancer and liver tissue (LV1401, US Biomax, MD) and normal human tissue arrays (T8234708-5, BioChain, CA) were rehydrated using a series of xylene and ethanol, followed by inactivation of endogenous catalase activity by incubation with 3% H2O2 for 15 min. Arrays were blocked with 5% BSA in Tris-buffered saline containing 0.05% Tween-20 (TBS-T) at 4°C overnight, and then they were incubated with 10 μg/mL mAb mixture in 1% BSA in TBS-T at 37°C for 45 min. After washing with TBS-T, the arrays were incubated with 1:2500 diluted HRP-conjugated anti-human IgG (Jackson ImmunoResearch Laboratories) at room temperature for 30 min. After washing with TBS-T, the arrays were visualized by staining with diaminobenzidine (ImmPACT DAB; VETOR Laboratories, CA) and counterstaining with Meyer's hematoxylin (Thermo Fisher Scientific) and captured using a CCD camera mounted on a microscope and its imaging software (BX53 and cellSense, Olympus, Tokyo, Japan). ..
Incubation:Article Title: Development of a recombinant hepatitis B immunoglobulin derived from B cells collected from healthy individuals administered with hepatitis B virus vaccines: A feasibility study.
Article Snippet: HBV infection was monitored by quantifying the HBV e antigen (HBeAg) in the culture medium with a commercial ELISA (UickTiterTM Hepatitis B “e” Antigen ELISA Kit, CELL BIOLABS, CA). .. Paraffin arrays of HBV human liver cancer and liver tissue (LV1401, US Biomax, MD) and normal human tissue arrays (T8234708-5, BioChain, CA) were rehydrated using a series of xylene and ethanol, followed by inactivation of endogenous catalase activity by incubation with 3% H2O2 for 15 min. Arrays were blocked with 5% BSA in Tris-buffered saline containing 0.05% Tween-20 (TBS-T) at 4°C overnight, and then they were incubated with 10 μg/mL mAb mixture in 1% BSA in TBS-T at 37°C for 45 min. After washing with TBS-T, the arrays were incubated with 1:2500 diluted HRP-conjugated anti-human IgG (Jackson ImmunoResearch Laboratories) at room temperature for 30 min. After washing with TBS-T, the arrays were visualized by staining with diaminobenzidine (ImmPACT DAB; VETOR Laboratories, CA) and counterstaining with Meyer's hematoxylin (Thermo Fisher Scientific) and captured using a CCD camera mounted on a microscope and its imaging software (BX53 and cellSense, Olympus, Tokyo, Japan). ..
Saline:Article Title: Development of a recombinant hepatitis B immunoglobulin derived from B cells collected from healthy individuals administered with hepatitis B virus vaccines: A feasibility study.
Article Snippet: HBV infection was monitored by quantifying the HBV e antigen (HBeAg) in the culture medium with a commercial ELISA (UickTiterTM Hepatitis B “e” Antigen ELISA Kit, CELL BIOLABS, CA). .. Paraffin arrays of HBV human liver cancer and liver tissue (LV1401, US Biomax, MD) and normal human tissue arrays (T8234708-5, BioChain, CA) were rehydrated using a series of xylene and ethanol, followed by inactivation of endogenous catalase activity by incubation with 3% H2O2 for 15 min. Arrays were blocked with 5% BSA in Tris-buffered saline containing 0.05% Tween-20 (TBS-T) at 4°C overnight, and then they were incubated with 10 μg/mL mAb mixture in 1% BSA in TBS-T at 37°C for 45 min. After washing with TBS-T, the arrays were incubated with 1:2500 diluted HRP-conjugated anti-human IgG (Jackson ImmunoResearch Laboratories) at room temperature for 30 min. After washing with TBS-T, the arrays were visualized by staining with diaminobenzidine (ImmPACT DAB; VETOR Laboratories, CA) and counterstaining with Meyer's hematoxylin (Thermo Fisher Scientific) and captured using a CCD camera mounted on a microscope and its imaging software (BX53 and cellSense, Olympus, Tokyo, Japan). ..
Staining:Article Title: Development of a recombinant hepatitis B immunoglobulin derived from B cells collected from healthy individuals administered with hepatitis B virus vaccines: A feasibility study.
Article Snippet: HBV infection was monitored by quantifying the HBV e antigen (HBeAg) in the culture medium with a commercial ELISA (UickTiterTM Hepatitis B “e” Antigen ELISA Kit, CELL BIOLABS, CA). .. Paraffin arrays of HBV human liver cancer and liver tissue (LV1401, US Biomax, MD) and normal human tissue arrays (T8234708-5, BioChain, CA) were rehydrated using a series of xylene and ethanol, followed by inactivation of endogenous catalase activity by incubation with 3% H2O2 for 15 min. Arrays were blocked with 5% BSA in Tris-buffered saline containing 0.05% Tween-20 (TBS-T) at 4°C overnight, and then they were incubated with 10 μg/mL mAb mixture in 1% BSA in TBS-T at 37°C for 45 min. After washing with TBS-T, the arrays were incubated with 1:2500 diluted HRP-conjugated anti-human IgG (Jackson ImmunoResearch Laboratories) at room temperature for 30 min. After washing with TBS-T, the arrays were visualized by staining with diaminobenzidine (ImmPACT DAB; VETOR Laboratories, CA) and counterstaining with Meyer's hematoxylin (Thermo Fisher Scientific) and captured using a CCD camera mounted on a microscope and its imaging software (BX53 and cellSense, Olympus, Tokyo, Japan). ..
Microscopy:Article Title: Development of a recombinant hepatitis B immunoglobulin derived from B cells collected from healthy individuals administered with hepatitis B virus vaccines: A feasibility study.
Article Snippet: HBV infection was monitored by quantifying the HBV e antigen (HBeAg) in the culture medium with a commercial ELISA (UickTiterTM Hepatitis B “e” Antigen ELISA Kit, CELL BIOLABS, CA). .. Paraffin arrays of HBV human liver cancer and liver tissue (LV1401, US Biomax, MD) and normal human tissue arrays (T8234708-5, BioChain, CA) were rehydrated using a series of xylene and ethanol, followed by inactivation of endogenous catalase activity by incubation with 3% H2O2 for 15 min. Arrays were blocked with 5% BSA in Tris-buffered saline containing 0.05% Tween-20 (TBS-T) at 4°C overnight, and then they were incubated with 10 μg/mL mAb mixture in 1% BSA in TBS-T at 37°C for 45 min. After washing with TBS-T, the arrays were incubated with 1:2500 diluted HRP-conjugated anti-human IgG (Jackson ImmunoResearch Laboratories) at room temperature for 30 min. After washing with TBS-T, the arrays were visualized by staining with diaminobenzidine (ImmPACT DAB; VETOR Laboratories, CA) and counterstaining with Meyer's hematoxylin (Thermo Fisher Scientific) and captured using a CCD camera mounted on a microscope and its imaging software (BX53 and cellSense, Olympus, Tokyo, Japan). ..
Imaging:Article Title: Development of a recombinant hepatitis B immunoglobulin derived from B cells collected from healthy individuals administered with hepatitis B virus vaccines: A feasibility study.
Article Snippet: HBV infection was monitored by quantifying the HBV e antigen (HBeAg) in the culture medium with a commercial ELISA (UickTiterTM Hepatitis B “e” Antigen ELISA Kit, CELL BIOLABS, CA). .. Paraffin arrays of HBV human liver cancer and liver tissue (LV1401, US Biomax, MD) and normal human tissue arrays (T8234708-5, BioChain, CA) were rehydrated using a series of xylene and ethanol, followed by inactivation of endogenous catalase activity by incubation with 3% H2O2 for 15 min. Arrays were blocked with 5% BSA in Tris-buffered saline containing 0.05% Tween-20 (TBS-T) at 4°C overnight, and then they were incubated with 10 μg/mL mAb mixture in 1% BSA in TBS-T at 37°C for 45 min. After washing with TBS-T, the arrays were incubated with 1:2500 diluted HRP-conjugated anti-human IgG (Jackson ImmunoResearch Laboratories) at room temperature for 30 min. After washing with TBS-T, the arrays were visualized by staining with diaminobenzidine (ImmPACT DAB; VETOR Laboratories, CA) and counterstaining with Meyer's hematoxylin (Thermo Fisher Scientific) and captured using a CCD camera mounted on a microscope and its imaging software (BX53 and cellSense, Olympus, Tokyo, Japan). ..
Software:Article Title: Development of a recombinant hepatitis B immunoglobulin derived from B cells collected from healthy individuals administered with hepatitis B virus vaccines: A feasibility study.
Article Snippet: HBV infection was monitored by quantifying the HBV e antigen (HBeAg) in the culture medium with a commercial ELISA (UickTiterTM Hepatitis B “e” Antigen ELISA Kit, CELL BIOLABS, CA). .. Paraffin arrays of HBV human liver cancer and liver tissue (LV1401, US Biomax, MD) and normal human tissue arrays (T8234708-5, BioChain, CA) were rehydrated using a series of xylene and ethanol, followed by inactivation of endogenous catalase activity by incubation with 3% H2O2 for 15 min. Arrays were blocked with 5% BSA in Tris-buffered saline containing 0.05% Tween-20 (TBS-T) at 4°C overnight, and then they were incubated with 10 μg/mL mAb mixture in 1% BSA in TBS-T at 37°C for 45 min. After washing with TBS-T, the arrays were incubated with 1:2500 diluted HRP-conjugated anti-human IgG (Jackson ImmunoResearch Laboratories) at room temperature for 30 min. After washing with TBS-T, the arrays were visualized by staining with diaminobenzidine (ImmPACT DAB; VETOR Laboratories, CA) and counterstaining with Meyer's hematoxylin (Thermo Fisher Scientific) and captured using a CCD camera mounted on a microscope and its imaging software (BX53 and cellSense, Olympus, Tokyo, Japan). ..
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