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healthy brain tissue  (OriGene)


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    Structured Review

    OriGene healthy brain tissue
    Healthy Brain Tissue, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+tissue+array/TissueScan%2C+Human+Brain+cDNA+Array/pm42236120-298-30-39
    Average 94 stars, based on 11 article reviews
    healthy brain tissue - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: ZC4H2, an XLID gene, is required for the generation of a specific subset of CNS interneurons.
    Article Snippet: .. Since both transcripts were present in fetal brain, we examined the level of expression in different regions of human brain using a Rapid Scan Human Brain Panel (Origene). ..

    Article Title: Saitohin gene and uses of same
    Article Snippet: .. Because tau and STH have a significant overlap in general tissue expression, an expanded study was performed using the Human Brain Rapid-Scana Panel (Origene) to determine the central nervous system (CNS) expression patterns, and to ascertain whether STH and tau are coordinately expressed in the brain. ..

    Article Title: Ptchd1 deficiency induces excitatory synaptic and cognitive dysfunctions in mouse
    Article Snippet: .. The expression profile of Ptchd1 was assessed using the TissueScan quantitative PCR-ready cDNA Arrays (Origene, Rockville, MD, USA; ). .. The Ptchd1 full-length sequence from IMAGE cDNA clone 40095445 (Source BioSience, Nottingham, UK) was used for green fluorescent protein (GFP) fusion, deletion constructs and pulldown strategy using the Pierce GST Protein Interaction Pull-down Kit (Fisher scientific, Illkirch, France) to detect, respectively, PTCHD1 subcellular localization and protein interactions ( ).

    Article Title: Vaspin is related to gender, puberty and deteriorating insulin sensitivity in children.
    Article Snippet: Department of Women and Child Health, University Hospital for Children & Adolescents, University of Leipzig, Leipzig, Germany; Leipzig Heart Center, University of Leipzig, Leipzig, Germany; Institute of Laboratory Medicine, Clinical Chemistry & Molecular Diagnostics, University of Leipzig, Leipzig, Germany; Interdisciplinary Center of Clinical Research, University of Leipzig, Leipzig, Germany and Department of Medicine, University of Leipzig, Leipzig, Germany

    Article Title: Novel CAR T cell blend targeting PDPN and GD2 to overcome glioblastoma heterogeneity.
    Article Snippet: Acquisitions were conducted using a Cytek Northern Lights system (Cytek). .. Subsequent data analysis was performed with the Data Analysis Software Suite for LEGENDplex. qPCR Pooled cDNA from healthy organs (Human MTC Panel I and II, Takara Bio, Kusatsu, Japan) and healthy brain tissue (TissueScan, Human Brain cDNA Array, HBRT501, OriGene, Rockyville, Maryland, USA) was analyzed for PDPN expression. .. Each sample was analyzed with the StepOnePlus Real- time PCR System using TaqMan PDPN Gene Expression Assays (Thermo Fisher Scientific, FAMMGB_PL, Hs00366766_m1) with GAPDH as an endogenous control (Thermo Fisher Scientific, VIC- MGB_PL, Hs99999905_m1).

    Article Title: A novel CAR T cell blend targeting PDPN and GD2 to overcome glioblastoma heterogeneity
    Article Snippet: Data were analyzed using FlowJo software v10.7.1 (BD). .. Pooled cDNA of healthy organ (Human MTCTM Panel I & II, Takara Bio Inc., Kusatsu, Japan) and healthy brain tissue (TissueScan, Human Brain cDNA Array, HBRT501, OriGene, Rockyville, MD, USA) was analyzed for PDPN expression. .. Each sample was analyzed with the StepOnePlus Real-time PCR System using TaqMan TM PDPN Gene Expression Assays (Thermo Fisher Scientific, FAM-MGB_PL, Hs00366766_m1) with GAPDH as endogenous control (Thermo Fisher Scientific, VIC-MGB_PL, Hs99999905_m1).

    Polymerase Chain Reaction:

    Article Title: Ptchd1 deficiency induces excitatory synaptic and cognitive dysfunctions in mouse
    Article Snippet: .. The expression profile of Ptchd1 was assessed using the TissueScan quantitative PCR-ready cDNA Arrays (Origene, Rockville, MD, USA; ). .. The Ptchd1 full-length sequence from IMAGE cDNA clone 40095445 (Source BioSience, Nottingham, UK) was used for green fluorescent protein (GFP) fusion, deletion constructs and pulldown strategy using the Pierce GST Protein Interaction Pull-down Kit (Fisher scientific, Illkirch, France) to detect, respectively, PTCHD1 subcellular localization and protein interactions ( ).

    Real-time Polymerase Chain Reaction:

    Article Title: Vaspin is related to gender, puberty and deteriorating insulin sensitivity in children.
    Article Snippet: Department of Women and Child Health, University Hospital for Children & Adolescents, University of Leipzig, Leipzig, Germany; Leipzig Heart Center, University of Leipzig, Leipzig, Germany; Institute of Laboratory Medicine, Clinical Chemistry & Molecular Diagnostics, University of Leipzig, Leipzig, Germany; Interdisciplinary Center of Clinical Research, University of Leipzig, Leipzig, Germany and Department of Medicine, University of Leipzig, Leipzig, Germany

    Article Title: Novel CAR T cell blend targeting PDPN and GD2 to overcome glioblastoma heterogeneity.
    Article Snippet: Acquisitions were conducted using a Cytek Northern Lights system (Cytek). .. Subsequent data analysis was performed with the Data Analysis Software Suite for LEGENDplex. qPCR Pooled cDNA from healthy organs (Human MTC Panel I and II, Takara Bio, Kusatsu, Japan) and healthy brain tissue (TissueScan, Human Brain cDNA Array, HBRT501, OriGene, Rockyville, Maryland, USA) was analyzed for PDPN expression. .. Each sample was analyzed with the StepOnePlus Real- time PCR System using TaqMan PDPN Gene Expression Assays (Thermo Fisher Scientific, FAMMGB_PL, Hs00366766_m1) with GAPDH as an endogenous control (Thermo Fisher Scientific, VIC- MGB_PL, Hs99999905_m1).

    Affinity Magnetic Separation:

    Article Title: Vaspin is related to gender, puberty and deteriorating insulin sensitivity in children.
    Article Snippet: Department of Women and Child Health, University Hospital for Children & Adolescents, University of Leipzig, Leipzig, Germany; Leipzig Heart Center, University of Leipzig, Leipzig, Germany; Institute of Laboratory Medicine, Clinical Chemistry & Molecular Diagnostics, University of Leipzig, Leipzig, Germany; Interdisciplinary Center of Clinical Research, University of Leipzig, Leipzig, Germany and Department of Medicine, University of Leipzig, Leipzig, Germany

    Software:

    Article Title: Novel CAR T cell blend targeting PDPN and GD2 to overcome glioblastoma heterogeneity.
    Article Snippet: Acquisitions were conducted using a Cytek Northern Lights system (Cytek). .. Subsequent data analysis was performed with the Data Analysis Software Suite for LEGENDplex. qPCR Pooled cDNA from healthy organs (Human MTC Panel I and II, Takara Bio, Kusatsu, Japan) and healthy brain tissue (TissueScan, Human Brain cDNA Array, HBRT501, OriGene, Rockyville, Maryland, USA) was analyzed for PDPN expression. .. Each sample was analyzed with the StepOnePlus Real- time PCR System using TaqMan PDPN Gene Expression Assays (Thermo Fisher Scientific, FAMMGB_PL, Hs00366766_m1) with GAPDH as an endogenous control (Thermo Fisher Scientific, VIC- MGB_PL, Hs99999905_m1).



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    CBX family genes expression in colon cancer. ( A ) CBX expression level by qRT-PCR in three colon cancer cell lines (CACO-2, SW480, and HCT 116) compared to a normal colon cell line (NMC 460D). ( B ) CBX gene expression level in a colon cancer cDNA <t>RT-qPCR</t> array consisting of tumor ( N = 40) and normal samples ( N = 8). ( C ) Expression analysis of CBX family genes of colon tumor in UALCAN dataset (Normal = 41, Primary Tumor = 286). The star symbol indicates statistical significance (**: p ≤ 0.01, ***: p ≤ 0.001, ****: p ≤ 0.0001).
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    Image Search Results


    (A) Representative IHC staining of ITGB3 in PDAC tissue microarrays (TMA) showing varying expression levels in non-cancer and cancer tissue sections. (B). High magnification IHC images demonstrating the expression of ITGB3 on normal pancreas and cancer cells with stromal regions. (D-I) . Scatter plots depicting the correlation between ITGB3 and key tumor-associated markers such as HIF-1α (D) , GLUT-1 (E) , VEGFA (F) , Vimentin (G) , COL1A1 (H) , and PECAM1 (I) . Pearson correlation coefficients (R) and p -values indicate statistically significant positive correlation between ITGB3 and these markers. Statistical significance was assessed using a two-tailed unpaired Student’s t-test. All quantitative data represent the mean ± SEM. ∗∗∗∗p < 0.0001.

    Journal: bioRxiv

    Article Title: ProAgio, a Novel Integrin αvβ3 Targeted Cytotoxin, Suppresses Tumor Growth and Reprograms the PDAC Microenvironment

    doi: 10.64898/2026.01.15.699725

    Figure Lengend Snippet: (A) Representative IHC staining of ITGB3 in PDAC tissue microarrays (TMA) showing varying expression levels in non-cancer and cancer tissue sections. (B). High magnification IHC images demonstrating the expression of ITGB3 on normal pancreas and cancer cells with stromal regions. (D-I) . Scatter plots depicting the correlation between ITGB3 and key tumor-associated markers such as HIF-1α (D) , GLUT-1 (E) , VEGFA (F) , Vimentin (G) , COL1A1 (H) , and PECAM1 (I) . Pearson correlation coefficients (R) and p -values indicate statistically significant positive correlation between ITGB3 and these markers. Statistical significance was assessed using a two-tailed unpaired Student’s t-test. All quantitative data represent the mean ± SEM. ∗∗∗∗p < 0.0001.

    Article Snippet: To evaluate the role of ITGB3 in PDAC, we analyzed its expression using IHC in a human PDAC tissue array (PA483-L97, Biomax) that contains 40 cancer and eight non-cancer tissues ( Supplementary Table 1 ).

    Techniques: Immunohistochemistry, Expressing, Two Tailed Test

    CBX family genes expression in colon cancer. ( A ) CBX expression level by qRT-PCR in three colon cancer cell lines (CACO-2, SW480, and HCT 116) compared to a normal colon cell line (NMC 460D). ( B ) CBX gene expression level in a colon cancer cDNA RT-qPCR array consisting of tumor ( N = 40) and normal samples ( N = 8). ( C ) Expression analysis of CBX family genes of colon tumor in UALCAN dataset (Normal = 41, Primary Tumor = 286). The star symbol indicates statistical significance (**: p ≤ 0.01, ***: p ≤ 0.001, ****: p ≤ 0.0001).

    Journal: International Journal of Molecular Sciences

    Article Title: The Study of Chromobox Protein Homolog 4 in 3D Organoid Models of Colon Cancer as a Potential Predictive Marker

    doi: 10.3390/ijms26157385

    Figure Lengend Snippet: CBX family genes expression in colon cancer. ( A ) CBX expression level by qRT-PCR in three colon cancer cell lines (CACO-2, SW480, and HCT 116) compared to a normal colon cell line (NMC 460D). ( B ) CBX gene expression level in a colon cancer cDNA RT-qPCR array consisting of tumor ( N = 40) and normal samples ( N = 8). ( C ) Expression analysis of CBX family genes of colon tumor in UALCAN dataset (Normal = 41, Primary Tumor = 286). The star symbol indicates statistical significance (**: p ≤ 0.01, ***: p ≤ 0.001, ****: p ≤ 0.0001).

    Article Snippet: Human TissueScan Colon Cancer Tissue qPCR Panel IV (HCRT304), containing first-strand cDNA from 48 samples covering 8-normal, 5-Stage I, 8-IIA, 1-II, 1-IIIA, 6-IIIB, 3-IIIC, 6-III, and 10-IV patients, was purchased from Origene (Rockville, MD, USA).

    Techniques: Expressing, Quantitative RT-PCR, Gene Expression

    Association between CBX4 expression levels and clinical stage and grade of CRC patients. ( A ) CBX4 expression analyses performed using the UALCAN dataset with relative statistical comparison among groups. ( B ) CBX4 expression analyses in a colon cancer cDNA RT-qPCR array consisting of tumor ( n = 40) and normal samples ( n = 8) stratifying patients according to stage and grade. The star symbol indicates statistical significance (**: p ≤ 0.01).

    Journal: International Journal of Molecular Sciences

    Article Title: The Study of Chromobox Protein Homolog 4 in 3D Organoid Models of Colon Cancer as a Potential Predictive Marker

    doi: 10.3390/ijms26157385

    Figure Lengend Snippet: Association between CBX4 expression levels and clinical stage and grade of CRC patients. ( A ) CBX4 expression analyses performed using the UALCAN dataset with relative statistical comparison among groups. ( B ) CBX4 expression analyses in a colon cancer cDNA RT-qPCR array consisting of tumor ( n = 40) and normal samples ( n = 8) stratifying patients according to stage and grade. The star symbol indicates statistical significance (**: p ≤ 0.01).

    Article Snippet: Human TissueScan Colon Cancer Tissue qPCR Panel IV (HCRT304), containing first-strand cDNA from 48 samples covering 8-normal, 5-Stage I, 8-IIA, 1-II, 1-IIIA, 6-IIIB, 3-IIIC, 6-III, and 10-IV patients, was purchased from Origene (Rockville, MD, USA).

    Techniques: Expressing, Comparison, Quantitative RT-PCR

    Characterization of CBX4 in CRC organoids. ( A ) Representative images of ex vivo PDO culture obtained from colorectal cancer biopsies are reported. Scale bar: 10 µm. ( B ) Real-time qPCR analysis of CBX4 in two groups of PDOs: the first one derived from healthy tissues (CNT) and the second one derived from tumor tissues (Tumor). Results were normalized to RPS18 mRNA and analyzed by 2 −ΔΔCt method. ( C ) Analysis of the knockdown efficiency of siCBX4 assessed by RT-PCR in PDOs after 72 h. ( D ) Effect of CBX4 silencing on PDO viability assessed by ATP Lite assay after 72 h. The star symbol indicates statistical significance (**: p ≤ 0.01).

    Journal: International Journal of Molecular Sciences

    Article Title: The Study of Chromobox Protein Homolog 4 in 3D Organoid Models of Colon Cancer as a Potential Predictive Marker

    doi: 10.3390/ijms26157385

    Figure Lengend Snippet: Characterization of CBX4 in CRC organoids. ( A ) Representative images of ex vivo PDO culture obtained from colorectal cancer biopsies are reported. Scale bar: 10 µm. ( B ) Real-time qPCR analysis of CBX4 in two groups of PDOs: the first one derived from healthy tissues (CNT) and the second one derived from tumor tissues (Tumor). Results were normalized to RPS18 mRNA and analyzed by 2 −ΔΔCt method. ( C ) Analysis of the knockdown efficiency of siCBX4 assessed by RT-PCR in PDOs after 72 h. ( D ) Effect of CBX4 silencing on PDO viability assessed by ATP Lite assay after 72 h. The star symbol indicates statistical significance (**: p ≤ 0.01).

    Article Snippet: Human TissueScan Colon Cancer Tissue qPCR Panel IV (HCRT304), containing first-strand cDNA from 48 samples covering 8-normal, 5-Stage I, 8-IIA, 1-II, 1-IIIA, 6-IIIB, 3-IIIC, 6-III, and 10-IV patients, was purchased from Origene (Rockville, MD, USA).

    Techniques: Ex Vivo, Derivative Assay, Knockdown, Reverse Transcription Polymerase Chain Reaction

    Role of CBX4 silencing in CRC organoids ( A , B ). Representative images of our target molecules through immunofluorescence analysis. Localization of NFkB in tumor ex vivo PDOs before (CNT) and after siCBX4. Maximal projection images of PDO incubated NFkB (green signal) and cell nuclei were stained with Hoechst 33342 (blue signal). Scale bar: 10 µm. ( C ) Real time qPCR analysis of NF-κB, c-myc, TNF-α, and IL-1 in tumor ex vivo PDOs before (CTR) and after CBX4 silencing. Results were normalized to RPS18 mRNA and analyzed by 2 −ΔΔCt method. The star symbol indicates statistical significance (*: p ≤ 0.05, **: p ≤ 0.01).

    Journal: International Journal of Molecular Sciences

    Article Title: The Study of Chromobox Protein Homolog 4 in 3D Organoid Models of Colon Cancer as a Potential Predictive Marker

    doi: 10.3390/ijms26157385

    Figure Lengend Snippet: Role of CBX4 silencing in CRC organoids ( A , B ). Representative images of our target molecules through immunofluorescence analysis. Localization of NFkB in tumor ex vivo PDOs before (CNT) and after siCBX4. Maximal projection images of PDO incubated NFkB (green signal) and cell nuclei were stained with Hoechst 33342 (blue signal). Scale bar: 10 µm. ( C ) Real time qPCR analysis of NF-κB, c-myc, TNF-α, and IL-1 in tumor ex vivo PDOs before (CTR) and after CBX4 silencing. Results were normalized to RPS18 mRNA and analyzed by 2 −ΔΔCt method. The star symbol indicates statistical significance (*: p ≤ 0.05, **: p ≤ 0.01).

    Article Snippet: Human TissueScan Colon Cancer Tissue qPCR Panel IV (HCRT304), containing first-strand cDNA from 48 samples covering 8-normal, 5-Stage I, 8-IIA, 1-II, 1-IIIA, 6-IIIB, 3-IIIC, 6-III, and 10-IV patients, was purchased from Origene (Rockville, MD, USA).

    Techniques: Immunofluorescence, Ex Vivo, Incubation, Staining