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human medulloblastoma tissue array slides  (Cybrdi Inc)

 
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    Structured Review

    Cybrdi Inc human medulloblastoma tissue array slides
    (A) CD1d expression in four <t>medulloblastoma</t> cell lines was examined by flow cytometry: staining with isotype control (tinted) or anti-hCD1d (open). (B) CD1d mRNA expression in four medulloblastoma cell lines was analyzed by RT-PCR using GADPH as an amplification control. (C) CD1d expression in primary tumors was assessed by IHC staining. Representative sections shown are from two CD1d-positive (upper pannel) and two CD1d-negative (lower pannel) of 20 analyzed tumors. Original magnification, X10. (D) CD1d mRNA expression was analyzed in 61 primary tumors by Taqman qRT-PCR and quantified using delta Ct method relative to the expression of HPRT1. DAOY and CHLA-255 cells were used as CD1d positive and negative controls, respectively.
    Human Medulloblastoma Tissue Array Slides, supplied by Cybrdi Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+tissue+array+slides/human+medulloblastoma+tissue+array+slides/pmc03809126-60-0-15
    Average 90 stars, based on 1 article reviews
    human medulloblastoma tissue array slides - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Medulloblastoma expresses CD1d and can be targeted for immunotherapy with NKT cells"

    Article Title: Medulloblastoma expresses CD1d and can be targeted for immunotherapy with NKT cells

    Journal: Clinical immunology (Orlando, Fla.)

    doi: 10.1016/j.clim.2013.06.005

    (A) CD1d expression in four medulloblastoma cell lines was examined by flow cytometry: staining with isotype control (tinted) or anti-hCD1d (open). (B) CD1d mRNA expression in four medulloblastoma cell lines was analyzed by RT-PCR using GADPH as an amplification control. (C) CD1d expression in primary tumors was assessed by IHC staining. Representative sections shown are from two CD1d-positive (upper pannel) and two CD1d-negative (lower pannel) of 20 analyzed tumors. Original magnification, X10. (D) CD1d mRNA expression was analyzed in 61 primary tumors by Taqman qRT-PCR and quantified using delta Ct method relative to the expression of HPRT1. DAOY and CHLA-255 cells were used as CD1d positive and negative controls, respectively.
    Figure Legend Snippet: (A) CD1d expression in four medulloblastoma cell lines was examined by flow cytometry: staining with isotype control (tinted) or anti-hCD1d (open). (B) CD1d mRNA expression in four medulloblastoma cell lines was analyzed by RT-PCR using GADPH as an amplification control. (C) CD1d expression in primary tumors was assessed by IHC staining. Representative sections shown are from two CD1d-positive (upper pannel) and two CD1d-negative (lower pannel) of 20 analyzed tumors. Original magnification, X10. (D) CD1d mRNA expression was analyzed in 61 primary tumors by Taqman qRT-PCR and quantified using delta Ct method relative to the expression of HPRT1. DAOY and CHLA-255 cells were used as CD1d positive and negative controls, respectively.

    Techniques Used: Expressing, Flow Cytometry, Staining, Control, Reverse Transcription Polymerase Chain Reaction, Amplification, Immunohistochemistry, Quantitative RT-PCR

    Related Articles

    Immunohistochemistry:

    Article Title: Medulloblastoma expresses CD1d and can be targeted for immunotherapy with NKT cells
    Article Snippet: .. Immunohistochemistry Human medulloblastoma tissue array slides and normal brain paraffin tissue sections were purchased from Cybrdi, Inc. H&E staining was done following a standard hematoxylin and eosin staining protocol. .. CD1d and CD3 expression was detected with immunohistochemistry using mouse anti-human CD1d mAb (clone NOR3.2, Abcam) and mouse anti-human CD3 mAb (clone PS-1, Vector Laboratories).

    Staining:

    Article Title: Medulloblastoma expresses CD1d and can be targeted for immunotherapy with NKT cells
    Article Snippet: .. Immunohistochemistry Human medulloblastoma tissue array slides and normal brain paraffin tissue sections were purchased from Cybrdi, Inc. H&E staining was done following a standard hematoxylin and eosin staining protocol. .. CD1d and CD3 expression was detected with immunohistochemistry using mouse anti-human CD1d mAb (clone NOR3.2, Abcam) and mouse anti-human CD3 mAb (clone PS-1, Vector Laboratories).

    Article Title: Medulloblastoma expresses CD1d and can be targeted for immunotherapy with NKT cells
    Article Snippet: .. Human medulloblastoma tissue array slides and normal brain paraffin tissue sections were purchased from Cybrdi, Inc. H&E staining was done following a standard hematoxylin and eosin staining protocol. .. CD1d and CD3 expression was detected with immunohistochemistry using mouse anti-human CD1d mAb (clone NOR3.2, Abcam) and mouse anti-human CD3 mAb (clone PS-1, Vector Laboratories).



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