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pe conjugated tim3 antibody  (R&D Systems)


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    Structured Review

    R&D Systems pe conjugated tim3 antibody
    Pe Conjugated Tim3 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+tim3/Human+TIM-3+PE-conjugated+Antibody/us12376573-1704-30-34
    Average 93 stars, based on 35 article reviews
    pe conjugated tim3 antibody - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    other:

    Article Title: Dexamethasone potentiates chimeric antigen receptor Tcell persistence and function by enhancing IL-7R expression
    Article Snippet: After 7 days, CD3/CD28 Dynabeads beads were removed using a DynaMag-5 Magnet (Invitrogen, 12303D) and 7 105 cells/mL were plated in RPMI 1640/10% FBS, 50 U/mL rhIL-2, and 0.5 ng/mL rhIL-15 to rest for 48 h. CAR T cells were cultured for 16–24 days before being used in in vitro experiments or being frozen in CryoStor (Biolife Solutions, 205102) for mouse experiments.

    Article Title: Treatment of cancer with anti-GITR agonist antibodies
    Article Snippet: The samples were then acquired on a Beckman Coulter Cytoflex S flow cytometer, and the resulting data was analyzed using FlowJo software (TreeStar).

    Article Title: Dexamethasone Potentiates Chimeric Antigen Receptor T Cell Persistence and Function by Enhancing IL-7Rα Expression.
    Article Snippet: Where indicated, media was also supplemented with IL-7 (R&D 318 Systems, 207-IL-025) on days 9, 12, and 14.

    Control:

    Article Title: Effects of Baicalin on the Galectin-9-tim-3 pathway in ovarian damage induced by X-ray irradiation and bacterial lipopolysaccharide in pregnant rats and their fetuses
    Article Snippet: FlowJo software (FlowJo, LLC, Ashland, OR, USA) was used for data analysis. .. The antibodies used included: PE-conjugated anti-Tim-3 (rat IgG2a, catalog no. FAB2365P, 50 μg/ml; R&D Systems, China), PerCpCy5.5-conjugated anti-CD4 (mouse monoclonal, catalog no. 45-0037- 42, 10 μg/ml; eBioscience), APC-conjugated anti-CD8 (mouse monoclonal, catalog no. 17-0086-42, 10 μg/ml; eBioscience), and a PEconjugated rat IgG2a isotype control (catalog no. IC006P, 50 μg/ml; R&D Systems, China). ..

    Staining:

    Article Title: TIM-3 signaling hijacks the canonical Wnt/β-catenin pathway to maintain cancer stemness in acute myeloid leukemia
    Article Snippet: .. Cells were stained with fluorescein isothiocyanate-conjugated anti-CD99 (HCD99; BioLegend), phosphatidylethanolamine-conjugated (PE-conjugated) anti-TIM-3 (344823; R&D Systems), allophycocyanin-conjugated anti-CD34 (8G12; BD Biosciences), anti-TIM-3 (F38-2E2; BioLegend), allophycocyanin/Cyanine-7-conjugated CD45 (HI30; BioLegend), Brilliant Violet 421-conjugated CD38 (HIT2; BD Biosciences), and Brilliant Violet 650conjugated CD3 (UCHT1; BioLegend). .. For the intracellular staining analysis, we used the BD cytofixTM fixation buffer and BD PhosflowTM Perm Buffer III according to the instructions provided by the manufacturer.

    Article Title: Genetically modified mice comprising humanized cellular immune system components with improved diversity of TCRB repertoire
    Article Snippet: Blood was taken from uninfected mice and infected humanized mice 3 weeks post-infection and stained using flow cytometry with PE-Cy7 conjugated anti-PD1 antibody (BIOLEGEND), PerCpCy5.5 conjugated Lag3 antibody (BIOLEGEND), and PE conjugated Tim3 antibody (R&D Systems). .. Blood was taken from uninfected mice and infected humanized mice 3 weeks post-infection and stained using flow cytometry with PE-Cy7 conjugated anti-PD1 antibody (BIOLEGEND), PerCpCy5.5 conjugated Lag3 antibody (BIOLEGEND), and PE conjugated Tim3 antibody (R&D Systems). ..

    Marker:

    Article Title: Baseline biomarkers of efficacy and on-treatment immune-profile changes associated with bempegaldesleukin plus nivolumab
    Article Snippet: .. Antibodies and fluorochromes used for immunophenotyping flow cytometric analysis FoxP3 Treg panel Marker Clone Fluorochrome Catalog Vendor CD45RA HI100 BB515 564552 BD Biosciences CD39 TU66 PerCP Cy5.5 564899 BD Biosciences FOXP3 PCH101 APC 17-4776-42 Thermo Fisher Scientific CD3 OKT3 AF700 317340 Biolegend CD45 2D1 APC-H7 560178 BD Biosciences CCR4 L291H4 BV421 359414 Biolegend CD4 SK3 BV510 344634 Biolegend HLA−DR G46-6 BV650 564231 BD Biosciences Ki67 B56 BV786 563756 BD Biosciences PD1 MIH4 PE 12-9969-42 Thermo Fisher Scientific CD25 CD25-4E3 PE eFluor610 61-0257-42 Thermo Fisher Scientific ICOS C398.4A PE-Cy7 313520 Biolegend T effector/memory panel Marker Clone Fluorochrome Catalog Vendor CD45RA HI100 BB515 564552 BD Biosciences Ki67 B56 PerCP-Cy5.5 561284 BD Biosciences CD27 O323 AF647 302812 Biolegend CD3 SK7 AF700 344822 Biolegend CD45 2D1 APC-H7 560178 BD Biosciences CD197 (CCR7) GO43H7 BV421 353208 Biolegend CD4 OKT4 BV510 300546 Biolegend CD8 SK1 BV605 564116 BD Biosciences HLA−DR G46-6 BV650 564231 BD Biosciences CD279 (PD-1) MIH4 PE 12-9969-42 Thermo Fisher Scientific CD152 BNI3 PE-CF594 562742 BD Biosciences CD38 HB7 PE-Cy7 356608 Biolegend NK panel Marker Clone Fluorochrome Catalog Vendor CD94 DX22 FITC 305504 Biolegend Ki67 B56 PerCP-Cy5.5 561284 BD Biosciences CD56 HCD56 AF647 318314 Biolegend CD3 SK7 AF700 344822 Biolegend CD45 2D1 APC-H7 560178 BD Biosciences CD335 (NKp46) 9E2 BV421 564065 BD Biosciences CD16 3G8 BV510 563830 BD Biosciences CD27 O323 BV605 302830 Biolegend HLA−DR G46-6 BV650 564231 BD Biosciences CD366 (TIM-3) 344823 PE FAB2365P R&D Systems CD314 (NKG2D) 1D11 PE-CF594 562498 BD Biosciences CD57 TB01 PE Cy-7 25-0577-42 Thermo Fisher Scientific Supplementary Figure 1. ..

    Infection:

    Article Title: Genetically modified mice comprising humanized cellular immune system components with improved diversity of TCRB repertoire
    Article Snippet: Blood was taken from uninfected mice and infected humanized mice 3 weeks post-infection and stained using flow cytometry with PE-Cy7 conjugated anti-PD1 antibody (BIOLEGEND), PerCpCy5.5 conjugated Lag3 antibody (BIOLEGEND), and PE conjugated Tim3 antibody (R&D Systems). .. Blood was taken from uninfected mice and infected humanized mice 3 weeks post-infection and stained using flow cytometry with PE-Cy7 conjugated anti-PD1 antibody (BIOLEGEND), PerCpCy5.5 conjugated Lag3 antibody (BIOLEGEND), and PE conjugated Tim3 antibody (R&D Systems). ..

    Flow Cytometry:

    Article Title: Genetically modified mice comprising humanized cellular immune system components with improved diversity of TCRB repertoire
    Article Snippet: Blood was taken from uninfected mice and infected humanized mice 3 weeks post-infection and stained using flow cytometry with PE-Cy7 conjugated anti-PD1 antibody (BIOLEGEND), PerCpCy5.5 conjugated Lag3 antibody (BIOLEGEND), and PE conjugated Tim3 antibody (R&D Systems). .. Blood was taken from uninfected mice and infected humanized mice 3 weeks post-infection and stained using flow cytometry with PE-Cy7 conjugated anti-PD1 antibody (BIOLEGEND), PerCpCy5.5 conjugated Lag3 antibody (BIOLEGEND), and PE conjugated Tim3 antibody (R&D Systems). ..

    Bioprocessing:

    Article Title: Evaluation of the Elements of Short Hairpin RNAs in Developing shRNA-Containing CAR T Cells
    Article Snippet: .. Fluorochrome-conjugated monoclonal antibodies are CD3 (BD Bioscience, San Jose, CA, USA, 563109, 557832), CD4 (BD Bioscience, 557852, 340133), CD8 (BD Biosciences, 348793), CD62L (BD Biosciences, 341012), CD127 (Biolegend, San Diego, CA, USA, 351319), EGFR (Biolegend, 352906), LAG3 (LSBio, Seattle, DC, USA, LS-B2237), PD1 (Invitrogen, Waltham, MA, USA, 47-2799-42), TIM3 (R&D Systems, FAB2365P), CD45RA (BD Biosciences, 555488), CD45RO (BD Biosciences, 561137), CD45 (BD Biosciences, 340665), CD107a (BD Biosciences, 555800), CD45 (BD Biosciences, 340665), or CD19 (Life Technologies, Carlsbad, CA, USA, MHCD1905). ..



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    (A) A UMAP showing the integration of tumour and benign cells of the TME, and healthy lymph node data from two public studies (PMID35027729, PMID39566559). Cells are coloured according to the data source, and scANVI-predicted cell types are shown in (B), (C) and (D). Images of an ALK+ ALCL case colored by Cell2location-predicted cell type abundances in each Visium spot. (C) Predictions for the locations of malignant cells, <t>TIM3+</t> T cells (T_TIM3+) and cytotoxic T cells (T_CD8+_cytotoxic), (D) Predictions for the locations of dark zone B cells (B_GC_DZ), light zone B cells (B_GC_LZ) and follicular dendritic cells (FDC). (E) A dotplot showing non-negative matrix factorisation (NMF) factors, where each factor is a group of co-localized cell types. In the dotplot, the size and color density represent the loading of each cell type in each factor. (F) Staining of TIM3 and CD30 expression on two representative ALK+ ALCL cases (G) Summary of the % TIM3+ infiltrating T lymphocytes in each of the 9 ALK+ ALCL cases analysed.
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    (A) A UMAP showing the integration of tumour and benign cells of the TME, and healthy lymph node data from two public studies (PMID35027729, PMID39566559). Cells are coloured according to the data source, and scANVI-predicted cell types are shown in (B), (C) and (D). Images of an ALK+ ALCL case colored by Cell2location-predicted cell type abundances in each Visium spot. (C) Predictions for the locations of malignant cells, <t>TIM3+</t> T cells (T_TIM3+) and cytotoxic T cells (T_CD8+_cytotoxic), (D) Predictions for the locations of dark zone B cells (B_GC_DZ), light zone B cells (B_GC_LZ) and follicular dendritic cells (FDC). (E) A dotplot showing non-negative matrix factorisation (NMF) factors, where each factor is a group of co-localized cell types. In the dotplot, the size and color density represent the loading of each cell type in each factor. (F) Staining of TIM3 and CD30 expression on two representative ALK+ ALCL cases (G) Summary of the % TIM3+ infiltrating T lymphocytes in each of the 9 ALK+ ALCL cases analysed.
    Anti Human Tim3 Rat Monoclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems pe conjugated tim3 antibody
    (A) A UMAP showing the integration of tumour and benign cells of the TME, and healthy lymph node data from two public studies (PMID35027729, PMID39566559). Cells are coloured according to the data source, and scANVI-predicted cell types are shown in (B), (C) and (D). Images of an ALK+ ALCL case colored by Cell2location-predicted cell type abundances in each Visium spot. (C) Predictions for the locations of malignant cells, <t>TIM3+</t> T cells (T_TIM3+) and cytotoxic T cells (T_CD8+_cytotoxic), (D) Predictions for the locations of dark zone B cells (B_GC_DZ), light zone B cells (B_GC_LZ) and follicular dendritic cells (FDC). (E) A dotplot showing non-negative matrix factorisation (NMF) factors, where each factor is a group of co-localized cell types. In the dotplot, the size and color density represent the loading of each cell type in each factor. (F) Staining of TIM3 and CD30 expression on two representative ALK+ ALCL cases (G) Summary of the % TIM3+ infiltrating T lymphocytes in each of the 9 ALK+ ALCL cases analysed.
    Pe Conjugated Tim3 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+tim3/Human+TIM-3+PE-conjugated+Antibody/us12376573-1704-30-34
    Average 93 stars, based on 1 article reviews
    pe conjugated tim3 antibody - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    Image Search Results


    (A) A UMAP showing the integration of tumour and benign cells of the TME, and healthy lymph node data from two public studies (PMID35027729, PMID39566559). Cells are coloured according to the data source, and scANVI-predicted cell types are shown in (B), (C) and (D). Images of an ALK+ ALCL case colored by Cell2location-predicted cell type abundances in each Visium spot. (C) Predictions for the locations of malignant cells, TIM3+ T cells (T_TIM3+) and cytotoxic T cells (T_CD8+_cytotoxic), (D) Predictions for the locations of dark zone B cells (B_GC_DZ), light zone B cells (B_GC_LZ) and follicular dendritic cells (FDC). (E) A dotplot showing non-negative matrix factorisation (NMF) factors, where each factor is a group of co-localized cell types. In the dotplot, the size and color density represent the loading of each cell type in each factor. (F) Staining of TIM3 and CD30 expression on two representative ALK+ ALCL cases (G) Summary of the % TIM3+ infiltrating T lymphocytes in each of the 9 ALK+ ALCL cases analysed.

    Journal: bioRxiv

    Article Title: Single-Cell Profiling Reveals Developmental Trajectories and identifies SYK and TIM3 as Targets in some T Cell Lymphomas

    doi: 10.64898/2026.03.27.714741

    Figure Lengend Snippet: (A) A UMAP showing the integration of tumour and benign cells of the TME, and healthy lymph node data from two public studies (PMID35027729, PMID39566559). Cells are coloured according to the data source, and scANVI-predicted cell types are shown in (B), (C) and (D). Images of an ALK+ ALCL case colored by Cell2location-predicted cell type abundances in each Visium spot. (C) Predictions for the locations of malignant cells, TIM3+ T cells (T_TIM3+) and cytotoxic T cells (T_CD8+_cytotoxic), (D) Predictions for the locations of dark zone B cells (B_GC_DZ), light zone B cells (B_GC_LZ) and follicular dendritic cells (FDC). (E) A dotplot showing non-negative matrix factorisation (NMF) factors, where each factor is a group of co-localized cell types. In the dotplot, the size and color density represent the loading of each cell type in each factor. (F) Staining of TIM3 and CD30 expression on two representative ALK+ ALCL cases (G) Summary of the % TIM3+ infiltrating T lymphocytes in each of the 9 ALK+ ALCL cases analysed.

    Article Snippet: Following heat-mediated antigen retrieval, consecutive sections were stained for TIM3 (MAB23652, R&D Systems) and CD30 (M0751, Agilent Dako) both at a 1:50 dilution.

    Techniques: Staining, Expressing