Review



human recombinant c-src enzyme  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Millipore human recombinant c-src enzyme
    Human Recombinant C Src Enzyme, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+src+enzyme/recombinant+human+c+src/pm09516477-45-2-9
    Average 90 stars, based on 1 article reviews
    human recombinant c-src enzyme - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    In Vitro:

    Article Title: Tyrosine Phosphorylation Regulates the Endocytosis and Surface Expression of GluN3A-Containing NMDA Receptors
    Article Snippet: In vitro phosphorylation was performed by incubating GST-proteins with 9 units of recombinant human c-Src (Millipore Biotechnology) and 5 μCi of [γ- 32 P]ATP (PerkinElmer) for 1 h at 37°C in 30 μl of phosphorylation buffer (50 m m Tris-HCl, pH 7.4, 3 m m MnCl 2 , 10 m m MgCl 2 ) including 83.33 μ m unlabeled ATP (Sigma).

    Article Title: Protein Kinase G Type Iα Activity in Human Ovarian Cancer Cells Significantly Contributes to Enhanced Src Activation and DNA Synthesis/Cell Proliferation
    Article Snippet: Authors' A Cancer Ins Vegas, Nev Gerontolog The Chines 4Reproduc Departmen University Research Major, De Mol Canc D ow naded rom http://aournals.org/m cr/article-p/578/3402560/578 Previously, we showed that basal activity of nitric oxide (NO)/cyclic GMP (cGMP)/protein kinase G (PKG) signaling pathway protects against spontaneous apoptosis and confers resistance to cisplatin-induced apoptosis in human ovarian cancer cells.. The present study determines whether basal PKG kinase activity regulates Src family kinase (SFK) activity and proliferation in these cells.. PKG-Iα was identified as predominant isoform in both OV2008 (cisplatin-sensitive, wild-type p53) and A2780cp (cisplatin-resistant, mutated p53) ovarian cancer cells.

    Article Title: ?5?1 Integrin Engagement Increases Large Conductance, Ca 2+ -activated K + Channel Current and Ca 2+ Sensitivity through c- src -mediated Channel Phosphorylation
    Article Snippet: For in vitro phosphorylation of expressed wild-type and mutant BK channels by human c- src tyrosine kinase, immunoprecipitated BK channels bound to protein A-Sepharose beads were resuspended in 10 m m HEPES, pH 7.6, and 1 m m dithiothreitol and then added to a reaction mixture containing (final) the following: 50 m m HEPES, 125 m m NaCl, 5 m m MgCl 2 , 1 m m dithiothreitol, 0.2 m m Na 3 VO 4 , 4 mg/ml p -nitrophenyl phosphate, and ∼15 units of partially purified recombinant human c- src- (UBI/Millipore), pH 7.6.

    Article Title: Tyrosine Phosphorylation Regulates the Endocytosis and Surface Expression of GluN3A-Containing NMDA Receptors
    Article Snippet: In vitro phosphorylation was performed by incubating GST-proteins with 9 units of recombinant human c-Src (Millipore Biotechnology) and 5 Ci of [ - 32P]ATP (PerkinElmer) for 1 h at 37°C in 30 l of phosphorylation buffer (50 mM Tris-HCl, pH 7.4, 3 mM MnCl2, 10 mM MgCl2) including 83.33 M unlabeled ATP (Sigma).

    Article Title: Trihydrophobin 1 Phosphorylation by c-Src Regulates MAPK/ERK Signaling and Cell Migration
    Article Snippet: Purified GST, GST-TH1 or GST-TH1 mutant (5 μg) was incubated with recombinant human c-Src (5 U, from Millipore, Bedford, MA) in 30 μl kinase buffer (10 mM HEPES, pH 7.5, 150 mM NaCl, 2.5 mM DTT, 0.01% Triton X-100, 10 mM MnCl 2 , 100 μM ATP) for 30 minutes at 30°C.

    Article Title: Characterization of Phospho-(Tyrosine)-Mimetic Calmodulin Mutants
    Article Snippet: Mouse monoclonal anti-phosphotyrosine antibody (clone 4G10, isotype IgG 2bκ ), recombinant c-Src (human) and mouse monoclonal anti-CaM antibody (isotype IgG 1 ) were obtained from Millipore (Billerica, MA).

    Article Title: Cell-Based Proteome Profiling of Potential Dasatinib Targets by Use of Affinity-Based Probes
    Article Snippet: Protein kinases (PKs) play an important role in the development and progression of cancer by regulating cell growth, survival, invasion, metastasis, and angiogenesis.. Dasatinib (BMS-354825), a dual Src/Abl inhibitor, is a promising therapeutic agent with oral bioavailability.. It has been used for the treatment of imatinib-resistant chronic myelogenous leukemia (CML).

    Article Title: Protein Kinase G Type Iα Activity in Human Ovarian Cancer Cells Significantly Contributes to Enhanced Src Activation and DNA Synthesis/Cell Proliferation
    Article Snippet: In vitro Phosphorylation Reaction of Recombinant Human PKG-Iα and Recombinant Human c-Src Interactions between recombinant human PKG-Iα and recombinant human c-Src (both from Calbiochem) were determined using in vitro experiments.

    Phospho-proteomics:

    Article Title: Tyrosine Phosphorylation Regulates the Endocytosis and Surface Expression of GluN3A-Containing NMDA Receptors
    Article Snippet: In vitro phosphorylation was performed by incubating GST-proteins with 9 units of recombinant human c-Src (Millipore Biotechnology) and 5 μCi of [γ- 32 P]ATP (PerkinElmer) for 1 h at 37°C in 30 μl of phosphorylation buffer (50 m m Tris-HCl, pH 7.4, 3 m m MnCl 2 , 10 m m MgCl 2 ) including 83.33 μ m unlabeled ATP (Sigma).

    Article Title: Protein Kinase G Type Iα Activity in Human Ovarian Cancer Cells Significantly Contributes to Enhanced Src Activation and DNA Synthesis/Cell Proliferation
    Article Snippet: Authors' A Cancer Ins Vegas, Nev Gerontolog The Chines 4Reproduc Departmen University Research Major, De Mol Canc D ow naded rom http://aournals.org/m cr/article-p/578/3402560/578 Previously, we showed that basal activity of nitric oxide (NO)/cyclic GMP (cGMP)/protein kinase G (PKG) signaling pathway protects against spontaneous apoptosis and confers resistance to cisplatin-induced apoptosis in human ovarian cancer cells.. The present study determines whether basal PKG kinase activity regulates Src family kinase (SFK) activity and proliferation in these cells.. PKG-Iα was identified as predominant isoform in both OV2008 (cisplatin-sensitive, wild-type p53) and A2780cp (cisplatin-resistant, mutated p53) ovarian cancer cells.

    Article Title: ?5?1 Integrin Engagement Increases Large Conductance, Ca 2+ -activated K + Channel Current and Ca 2+ Sensitivity through c- src -mediated Channel Phosphorylation
    Article Snippet: For in vitro phosphorylation of expressed wild-type and mutant BK channels by human c- src tyrosine kinase, immunoprecipitated BK channels bound to protein A-Sepharose beads were resuspended in 10 m m HEPES, pH 7.6, and 1 m m dithiothreitol and then added to a reaction mixture containing (final) the following: 50 m m HEPES, 125 m m NaCl, 5 m m MgCl 2 , 1 m m dithiothreitol, 0.2 m m Na 3 VO 4 , 4 mg/ml p -nitrophenyl phosphate, and ∼15 units of partially purified recombinant human c- src- (UBI/Millipore), pH 7.6.

    Article Title: Tyrosine Phosphorylation Regulates the Endocytosis and Surface Expression of GluN3A-Containing NMDA Receptors
    Article Snippet: In vitro phosphorylation was performed by incubating GST-proteins with 9 units of recombinant human c-Src (Millipore Biotechnology) and 5 Ci of [ - 32P]ATP (PerkinElmer) for 1 h at 37°C in 30 l of phosphorylation buffer (50 mM Tris-HCl, pH 7.4, 3 mM MnCl2, 10 mM MgCl2) including 83.33 M unlabeled ATP (Sigma).

    Article Title: Trihydrophobin 1 Phosphorylation by c-Src Regulates MAPK/ERK Signaling and Cell Migration
    Article Snippet: Purified GST, GST-TH1 or GST-TH1 mutant (5 μg) was incubated with recombinant human c-Src (5 U, from Millipore, Bedford, MA) in 30 μl kinase buffer (10 mM HEPES, pH 7.5, 150 mM NaCl, 2.5 mM DTT, 0.01% Triton X-100, 10 mM MnCl 2 , 100 μM ATP) for 30 minutes at 30°C.

    Article Title: Characterization of Phospho-(Tyrosine)-Mimetic Calmodulin Mutants
    Article Snippet: Mouse monoclonal anti-phosphotyrosine antibody (clone 4G10, isotype IgG 2bκ ), recombinant c-Src (human) and mouse monoclonal anti-CaM antibody (isotype IgG 1 ) were obtained from Millipore (Billerica, MA).

    Article Title: Cell-Based Proteome Profiling of Potential Dasatinib Targets by Use of Affinity-Based Probes
    Article Snippet: Protein kinases (PKs) play an important role in the development and progression of cancer by regulating cell growth, survival, invasion, metastasis, and angiogenesis.. Dasatinib (BMS-354825), a dual Src/Abl inhibitor, is a promising therapeutic agent with oral bioavailability.. It has been used for the treatment of imatinib-resistant chronic myelogenous leukemia (CML).

    Article Title: Protein Kinase G Type Iα Activity in Human Ovarian Cancer Cells Significantly Contributes to Enhanced Src Activation and DNA Synthesis/Cell Proliferation
    Article Snippet: In vitro Phosphorylation Reaction of Recombinant Human PKG-Iα and Recombinant Human c-Src Interactions between recombinant human PKG-Iα and recombinant human c-Src (both from Calbiochem) were determined using in vitro experiments.

    Recombinant:

    Article Title: Tyrosine Phosphorylation Regulates the Endocytosis and Surface Expression of GluN3A-Containing NMDA Receptors
    Article Snippet: In vitro phosphorylation was performed by incubating GST-proteins with 9 units of recombinant human c-Src (Millipore Biotechnology) and 5 μCi of [γ- 32 P]ATP (PerkinElmer) for 1 h at 37°C in 30 μl of phosphorylation buffer (50 m m Tris-HCl, pH 7.4, 3 m m MnCl 2 , 10 m m MgCl 2 ) including 83.33 μ m unlabeled ATP (Sigma).

    Article Title: Protein Kinase G Type Iα Activity in Human Ovarian Cancer Cells Significantly Contributes to Enhanced Src Activation and DNA Synthesis/Cell Proliferation
    Article Snippet: Authors' A Cancer Ins Vegas, Nev Gerontolog The Chines 4Reproduc Departmen University Research Major, De Mol Canc D ow naded rom http://aournals.org/m cr/article-p/578/3402560/578 Previously, we showed that basal activity of nitric oxide (NO)/cyclic GMP (cGMP)/protein kinase G (PKG) signaling pathway protects against spontaneous apoptosis and confers resistance to cisplatin-induced apoptosis in human ovarian cancer cells.. The present study determines whether basal PKG kinase activity regulates Src family kinase (SFK) activity and proliferation in these cells.. PKG-Iα was identified as predominant isoform in both OV2008 (cisplatin-sensitive, wild-type p53) and A2780cp (cisplatin-resistant, mutated p53) ovarian cancer cells.

    Article Title: ?5?1 Integrin Engagement Increases Large Conductance, Ca 2+ -activated K + Channel Current and Ca 2+ Sensitivity through c- src -mediated Channel Phosphorylation
    Article Snippet: For in vitro phosphorylation of expressed wild-type and mutant BK channels by human c- src tyrosine kinase, immunoprecipitated BK channels bound to protein A-Sepharose beads were resuspended in 10 m m HEPES, pH 7.6, and 1 m m dithiothreitol and then added to a reaction mixture containing (final) the following: 50 m m HEPES, 125 m m NaCl, 5 m m MgCl 2 , 1 m m dithiothreitol, 0.2 m m Na 3 VO 4 , 4 mg/ml p -nitrophenyl phosphate, and ∼15 units of partially purified recombinant human c- src- (UBI/Millipore), pH 7.6.

    Article Title: Tyrosine Phosphorylation Regulates the Endocytosis and Surface Expression of GluN3A-Containing NMDA Receptors
    Article Snippet: In vitro phosphorylation was performed by incubating GST-proteins with 9 units of recombinant human c-Src (Millipore Biotechnology) and 5 Ci of [ - 32P]ATP (PerkinElmer) for 1 h at 37°C in 30 l of phosphorylation buffer (50 mM Tris-HCl, pH 7.4, 3 mM MnCl2, 10 mM MgCl2) including 83.33 M unlabeled ATP (Sigma).

    Article Title: Trihydrophobin 1 Phosphorylation by c-Src Regulates MAPK/ERK Signaling and Cell Migration
    Article Snippet: Purified GST, GST-TH1 or GST-TH1 mutant (5 μg) was incubated with recombinant human c-Src (5 U, from Millipore, Bedford, MA) in 30 μl kinase buffer (10 mM HEPES, pH 7.5, 150 mM NaCl, 2.5 mM DTT, 0.01% Triton X-100, 10 mM MnCl 2 , 100 μM ATP) for 30 minutes at 30°C.

    Article Title: Characterization of Phospho-(Tyrosine)-Mimetic Calmodulin Mutants
    Article Snippet: Mouse monoclonal anti-phosphotyrosine antibody (clone 4G10, isotype IgG 2bκ ), recombinant c-Src (human) and mouse monoclonal anti-CaM antibody (isotype IgG 1 ) were obtained from Millipore (Billerica, MA).

    Article Title: Cell-Based Proteome Profiling of Potential Dasatinib Targets by Use of Affinity-Based Probes
    Article Snippet: Protein kinases (PKs) play an important role in the development and progression of cancer by regulating cell growth, survival, invasion, metastasis, and angiogenesis.. Dasatinib (BMS-354825), a dual Src/Abl inhibitor, is a promising therapeutic agent with oral bioavailability.. It has been used for the treatment of imatinib-resistant chronic myelogenous leukemia (CML).

    Article Title: Protein Kinase G Type Iα Activity in Human Ovarian Cancer Cells Significantly Contributes to Enhanced Src Activation and DNA Synthesis/Cell Proliferation
    Article Snippet: In vitro Phosphorylation Reaction of Recombinant Human PKG-Iα and Recombinant Human c-Src Interactions between recombinant human PKG-Iα and recombinant human c-Src (both from Calbiochem) were determined using in vitro experiments.

    Plasmid Preparation:

    Article Title: Tyrosine Phosphorylation Regulates the Endocytosis and Surface Expression of GluN3A-Containing NMDA Receptors
    Article Snippet: In vitro phosphorylation was performed by incubating GST-proteins with 9 units of recombinant human c-Src (Millipore Biotechnology) and 5 μCi of [γ- 32 P]ATP (PerkinElmer) for 1 h at 37°C in 30 μl of phosphorylation buffer (50 m m Tris-HCl, pH 7.4, 3 m m MnCl 2 , 10 m m MgCl 2 ) including 83.33 μ m unlabeled ATP (Sigma).

    Article Title: Protein Kinase G Type Iα Activity in Human Ovarian Cancer Cells Significantly Contributes to Enhanced Src Activation and DNA Synthesis/Cell Proliferation
    Article Snippet: Authors' A Cancer Ins Vegas, Nev Gerontolog The Chines 4Reproduc Departmen University Research Major, De Mol Canc D ow naded rom http://aournals.org/m cr/article-p/578/3402560/578 Previously, we showed that basal activity of nitric oxide (NO)/cyclic GMP (cGMP)/protein kinase G (PKG) signaling pathway protects against spontaneous apoptosis and confers resistance to cisplatin-induced apoptosis in human ovarian cancer cells.. The present study determines whether basal PKG kinase activity regulates Src family kinase (SFK) activity and proliferation in these cells.. PKG-Iα was identified as predominant isoform in both OV2008 (cisplatin-sensitive, wild-type p53) and A2780cp (cisplatin-resistant, mutated p53) ovarian cancer cells.

    Article Title: ?5?1 Integrin Engagement Increases Large Conductance, Ca 2+ -activated K + Channel Current and Ca 2+ Sensitivity through c- src -mediated Channel Phosphorylation
    Article Snippet: For in vitro phosphorylation of expressed wild-type and mutant BK channels by human c- src tyrosine kinase, immunoprecipitated BK channels bound to protein A-Sepharose beads were resuspended in 10 m m HEPES, pH 7.6, and 1 m m dithiothreitol and then added to a reaction mixture containing (final) the following: 50 m m HEPES, 125 m m NaCl, 5 m m MgCl 2 , 1 m m dithiothreitol, 0.2 m m Na 3 VO 4 , 4 mg/ml p -nitrophenyl phosphate, and ∼15 units of partially purified recombinant human c- src- (UBI/Millipore), pH 7.6.

    Article Title: Tyrosine Phosphorylation Regulates the Endocytosis and Surface Expression of GluN3A-Containing NMDA Receptors
    Article Snippet: In vitro phosphorylation was performed by incubating GST-proteins with 9 units of recombinant human c-Src (Millipore Biotechnology) and 5 Ci of [ - 32P]ATP (PerkinElmer) for 1 h at 37°C in 30 l of phosphorylation buffer (50 mM Tris-HCl, pH 7.4, 3 mM MnCl2, 10 mM MgCl2) including 83.33 M unlabeled ATP (Sigma).

    Article Title: Trihydrophobin 1 Phosphorylation by c-Src Regulates MAPK/ERK Signaling and Cell Migration
    Article Snippet: Purified GST, GST-TH1 or GST-TH1 mutant (5 μg) was incubated with recombinant human c-Src (5 U, from Millipore, Bedford, MA) in 30 μl kinase buffer (10 mM HEPES, pH 7.5, 150 mM NaCl, 2.5 mM DTT, 0.01% Triton X-100, 10 mM MnCl 2 , 100 μM ATP) for 30 minutes at 30°C.

    Article Title: Characterization of Phospho-(Tyrosine)-Mimetic Calmodulin Mutants
    Article Snippet: Mouse monoclonal anti-phosphotyrosine antibody (clone 4G10, isotype IgG 2bκ ), recombinant c-Src (human) and mouse monoclonal anti-CaM antibody (isotype IgG 1 ) were obtained from Millipore (Billerica, MA).

    Article Title: Cell-Based Proteome Profiling of Potential Dasatinib Targets by Use of Affinity-Based Probes
    Article Snippet: Protein kinases (PKs) play an important role in the development and progression of cancer by regulating cell growth, survival, invasion, metastasis, and angiogenesis.. Dasatinib (BMS-354825), a dual Src/Abl inhibitor, is a promising therapeutic agent with oral bioavailability.. It has been used for the treatment of imatinib-resistant chronic myelogenous leukemia (CML).

    Article Title: Protein Kinase G Type Iα Activity in Human Ovarian Cancer Cells Significantly Contributes to Enhanced Src Activation and DNA Synthesis/Cell Proliferation
    Article Snippet: In vitro Phosphorylation Reaction of Recombinant Human PKG-Iα and Recombinant Human c-Src Interactions between recombinant human PKG-Iα and recombinant human c-Src (both from Calbiochem) were determined using in vitro experiments.

    Purification:

    Article Title: Tyrosine Phosphorylation Regulates the Endocytosis and Surface Expression of GluN3A-Containing NMDA Receptors
    Article Snippet: In vitro phosphorylation was performed by incubating GST-proteins with 9 units of recombinant human c-Src (Millipore Biotechnology) and 5 μCi of [γ- 32 P]ATP (PerkinElmer) for 1 h at 37°C in 30 μl of phosphorylation buffer (50 m m Tris-HCl, pH 7.4, 3 m m MnCl 2 , 10 m m MgCl 2 ) including 83.33 μ m unlabeled ATP (Sigma).

    Article Title: Protein Kinase G Type Iα Activity in Human Ovarian Cancer Cells Significantly Contributes to Enhanced Src Activation and DNA Synthesis/Cell Proliferation
    Article Snippet: Authors' A Cancer Ins Vegas, Nev Gerontolog The Chines 4Reproduc Departmen University Research Major, De Mol Canc D ow naded rom http://aournals.org/m cr/article-p/578/3402560/578 Previously, we showed that basal activity of nitric oxide (NO)/cyclic GMP (cGMP)/protein kinase G (PKG) signaling pathway protects against spontaneous apoptosis and confers resistance to cisplatin-induced apoptosis in human ovarian cancer cells.. The present study determines whether basal PKG kinase activity regulates Src family kinase (SFK) activity and proliferation in these cells.. PKG-Iα was identified as predominant isoform in both OV2008 (cisplatin-sensitive, wild-type p53) and A2780cp (cisplatin-resistant, mutated p53) ovarian cancer cells.

    Article Title: ?5?1 Integrin Engagement Increases Large Conductance, Ca 2+ -activated K + Channel Current and Ca 2+ Sensitivity through c- src -mediated Channel Phosphorylation
    Article Snippet: For in vitro phosphorylation of expressed wild-type and mutant BK channels by human c- src tyrosine kinase, immunoprecipitated BK channels bound to protein A-Sepharose beads were resuspended in 10 m m HEPES, pH 7.6, and 1 m m dithiothreitol and then added to a reaction mixture containing (final) the following: 50 m m HEPES, 125 m m NaCl, 5 m m MgCl 2 , 1 m m dithiothreitol, 0.2 m m Na 3 VO 4 , 4 mg/ml p -nitrophenyl phosphate, and ∼15 units of partially purified recombinant human c- src- (UBI/Millipore), pH 7.6.

    Article Title: Tyrosine Phosphorylation Regulates the Endocytosis and Surface Expression of GluN3A-Containing NMDA Receptors
    Article Snippet: In vitro phosphorylation was performed by incubating GST-proteins with 9 units of recombinant human c-Src (Millipore Biotechnology) and 5 Ci of [ - 32P]ATP (PerkinElmer) for 1 h at 37°C in 30 l of phosphorylation buffer (50 mM Tris-HCl, pH 7.4, 3 mM MnCl2, 10 mM MgCl2) including 83.33 M unlabeled ATP (Sigma).

    Article Title: Trihydrophobin 1 Phosphorylation by c-Src Regulates MAPK/ERK Signaling and Cell Migration
    Article Snippet: Purified GST, GST-TH1 or GST-TH1 mutant (5 μg) was incubated with recombinant human c-Src (5 U, from Millipore, Bedford, MA) in 30 μl kinase buffer (10 mM HEPES, pH 7.5, 150 mM NaCl, 2.5 mM DTT, 0.01% Triton X-100, 10 mM MnCl 2 , 100 μM ATP) for 30 minutes at 30°C.

    Article Title: Characterization of Phospho-(Tyrosine)-Mimetic Calmodulin Mutants
    Article Snippet: Mouse monoclonal anti-phosphotyrosine antibody (clone 4G10, isotype IgG 2bκ ), recombinant c-Src (human) and mouse monoclonal anti-CaM antibody (isotype IgG 1 ) were obtained from Millipore (Billerica, MA).

    Article Title: Cell-Based Proteome Profiling of Potential Dasatinib Targets by Use of Affinity-Based Probes
    Article Snippet: Protein kinases (PKs) play an important role in the development and progression of cancer by regulating cell growth, survival, invasion, metastasis, and angiogenesis.. Dasatinib (BMS-354825), a dual Src/Abl inhibitor, is a promising therapeutic agent with oral bioavailability.. It has been used for the treatment of imatinib-resistant chronic myelogenous leukemia (CML).

    Article Title: Protein Kinase G Type Iα Activity in Human Ovarian Cancer Cells Significantly Contributes to Enhanced Src Activation and DNA Synthesis/Cell Proliferation
    Article Snippet: In vitro Phosphorylation Reaction of Recombinant Human PKG-Iα and Recombinant Human c-Src Interactions between recombinant human PKG-Iα and recombinant human c-Src (both from Calbiochem) were determined using in vitro experiments.

    Mutagenesis:

    Article Title: Tyrosine Phosphorylation Regulates the Endocytosis and Surface Expression of GluN3A-Containing NMDA Receptors
    Article Snippet: In vitro phosphorylation was performed by incubating GST-proteins with 9 units of recombinant human c-Src (Millipore Biotechnology) and 5 μCi of [γ- 32 P]ATP (PerkinElmer) for 1 h at 37°C in 30 μl of phosphorylation buffer (50 m m Tris-HCl, pH 7.4, 3 m m MnCl 2 , 10 m m MgCl 2 ) including 83.33 μ m unlabeled ATP (Sigma).

    Article Title: Protein Kinase G Type Iα Activity in Human Ovarian Cancer Cells Significantly Contributes to Enhanced Src Activation and DNA Synthesis/Cell Proliferation
    Article Snippet: Authors' A Cancer Ins Vegas, Nev Gerontolog The Chines 4Reproduc Departmen University Research Major, De Mol Canc D ow naded rom http://aournals.org/m cr/article-p/578/3402560/578 Previously, we showed that basal activity of nitric oxide (NO)/cyclic GMP (cGMP)/protein kinase G (PKG) signaling pathway protects against spontaneous apoptosis and confers resistance to cisplatin-induced apoptosis in human ovarian cancer cells.. The present study determines whether basal PKG kinase activity regulates Src family kinase (SFK) activity and proliferation in these cells.. PKG-Iα was identified as predominant isoform in both OV2008 (cisplatin-sensitive, wild-type p53) and A2780cp (cisplatin-resistant, mutated p53) ovarian cancer cells.

    Article Title: ?5?1 Integrin Engagement Increases Large Conductance, Ca 2+ -activated K + Channel Current and Ca 2+ Sensitivity through c- src -mediated Channel Phosphorylation
    Article Snippet: For in vitro phosphorylation of expressed wild-type and mutant BK channels by human c- src tyrosine kinase, immunoprecipitated BK channels bound to protein A-Sepharose beads were resuspended in 10 m m HEPES, pH 7.6, and 1 m m dithiothreitol and then added to a reaction mixture containing (final) the following: 50 m m HEPES, 125 m m NaCl, 5 m m MgCl 2 , 1 m m dithiothreitol, 0.2 m m Na 3 VO 4 , 4 mg/ml p -nitrophenyl phosphate, and ∼15 units of partially purified recombinant human c- src- (UBI/Millipore), pH 7.6.

    Article Title: Tyrosine Phosphorylation Regulates the Endocytosis and Surface Expression of GluN3A-Containing NMDA Receptors
    Article Snippet: In vitro phosphorylation was performed by incubating GST-proteins with 9 units of recombinant human c-Src (Millipore Biotechnology) and 5 Ci of [ - 32P]ATP (PerkinElmer) for 1 h at 37°C in 30 l of phosphorylation buffer (50 mM Tris-HCl, pH 7.4, 3 mM MnCl2, 10 mM MgCl2) including 83.33 M unlabeled ATP (Sigma).

    Article Title: Trihydrophobin 1 Phosphorylation by c-Src Regulates MAPK/ERK Signaling and Cell Migration
    Article Snippet: Purified GST, GST-TH1 or GST-TH1 mutant (5 μg) was incubated with recombinant human c-Src (5 U, from Millipore, Bedford, MA) in 30 μl kinase buffer (10 mM HEPES, pH 7.5, 150 mM NaCl, 2.5 mM DTT, 0.01% Triton X-100, 10 mM MnCl 2 , 100 μM ATP) for 30 minutes at 30°C.

    Article Title: Characterization of Phospho-(Tyrosine)-Mimetic Calmodulin Mutants
    Article Snippet: Mouse monoclonal anti-phosphotyrosine antibody (clone 4G10, isotype IgG 2bκ ), recombinant c-Src (human) and mouse monoclonal anti-CaM antibody (isotype IgG 1 ) were obtained from Millipore (Billerica, MA).

    Article Title: Cell-Based Proteome Profiling of Potential Dasatinib Targets by Use of Affinity-Based Probes
    Article Snippet: Protein kinases (PKs) play an important role in the development and progression of cancer by regulating cell growth, survival, invasion, metastasis, and angiogenesis.. Dasatinib (BMS-354825), a dual Src/Abl inhibitor, is a promising therapeutic agent with oral bioavailability.. It has been used for the treatment of imatinib-resistant chronic myelogenous leukemia (CML).

    Article Title: Protein Kinase G Type Iα Activity in Human Ovarian Cancer Cells Significantly Contributes to Enhanced Src Activation and DNA Synthesis/Cell Proliferation
    Article Snippet: In vitro Phosphorylation Reaction of Recombinant Human PKG-Iα and Recombinant Human c-Src Interactions between recombinant human PKG-Iα and recombinant human c-Src (both from Calbiochem) were determined using in vitro experiments.

    Incubation:

    Article Title: Tyrosine Phosphorylation Regulates the Endocytosis and Surface Expression of GluN3A-Containing NMDA Receptors
    Article Snippet: In vitro phosphorylation was performed by incubating GST-proteins with 9 units of recombinant human c-Src (Millipore Biotechnology) and 5 μCi of [γ- 32 P]ATP (PerkinElmer) for 1 h at 37°C in 30 μl of phosphorylation buffer (50 m m Tris-HCl, pH 7.4, 3 m m MnCl 2 , 10 m m MgCl 2 ) including 83.33 μ m unlabeled ATP (Sigma).

    Article Title: Protein Kinase G Type Iα Activity in Human Ovarian Cancer Cells Significantly Contributes to Enhanced Src Activation and DNA Synthesis/Cell Proliferation
    Article Snippet: Authors' A Cancer Ins Vegas, Nev Gerontolog The Chines 4Reproduc Departmen University Research Major, De Mol Canc D ow naded rom http://aournals.org/m cr/article-p/578/3402560/578 Previously, we showed that basal activity of nitric oxide (NO)/cyclic GMP (cGMP)/protein kinase G (PKG) signaling pathway protects against spontaneous apoptosis and confers resistance to cisplatin-induced apoptosis in human ovarian cancer cells.. The present study determines whether basal PKG kinase activity regulates Src family kinase (SFK) activity and proliferation in these cells.. PKG-Iα was identified as predominant isoform in both OV2008 (cisplatin-sensitive, wild-type p53) and A2780cp (cisplatin-resistant, mutated p53) ovarian cancer cells.

    Article Title: ?5?1 Integrin Engagement Increases Large Conductance, Ca 2+ -activated K + Channel Current and Ca 2+ Sensitivity through c- src -mediated Channel Phosphorylation
    Article Snippet: For in vitro phosphorylation of expressed wild-type and mutant BK channels by human c- src tyrosine kinase, immunoprecipitated BK channels bound to protein A-Sepharose beads were resuspended in 10 m m HEPES, pH 7.6, and 1 m m dithiothreitol and then added to a reaction mixture containing (final) the following: 50 m m HEPES, 125 m m NaCl, 5 m m MgCl 2 , 1 m m dithiothreitol, 0.2 m m Na 3 VO 4 , 4 mg/ml p -nitrophenyl phosphate, and ∼15 units of partially purified recombinant human c- src- (UBI/Millipore), pH 7.6.

    Article Title: Tyrosine Phosphorylation Regulates the Endocytosis and Surface Expression of GluN3A-Containing NMDA Receptors
    Article Snippet: In vitro phosphorylation was performed by incubating GST-proteins with 9 units of recombinant human c-Src (Millipore Biotechnology) and 5 Ci of [ - 32P]ATP (PerkinElmer) for 1 h at 37°C in 30 l of phosphorylation buffer (50 mM Tris-HCl, pH 7.4, 3 mM MnCl2, 10 mM MgCl2) including 83.33 M unlabeled ATP (Sigma).

    Article Title: Trihydrophobin 1 Phosphorylation by c-Src Regulates MAPK/ERK Signaling and Cell Migration
    Article Snippet: Purified GST, GST-TH1 or GST-TH1 mutant (5 μg) was incubated with recombinant human c-Src (5 U, from Millipore, Bedford, MA) in 30 μl kinase buffer (10 mM HEPES, pH 7.5, 150 mM NaCl, 2.5 mM DTT, 0.01% Triton X-100, 10 mM MnCl 2 , 100 μM ATP) for 30 minutes at 30°C.

    Article Title: Characterization of Phospho-(Tyrosine)-Mimetic Calmodulin Mutants
    Article Snippet: Mouse monoclonal anti-phosphotyrosine antibody (clone 4G10, isotype IgG 2bκ ), recombinant c-Src (human) and mouse monoclonal anti-CaM antibody (isotype IgG 1 ) were obtained from Millipore (Billerica, MA).

    Article Title: Cell-Based Proteome Profiling of Potential Dasatinib Targets by Use of Affinity-Based Probes
    Article Snippet: Protein kinases (PKs) play an important role in the development and progression of cancer by regulating cell growth, survival, invasion, metastasis, and angiogenesis.. Dasatinib (BMS-354825), a dual Src/Abl inhibitor, is a promising therapeutic agent with oral bioavailability.. It has been used for the treatment of imatinib-resistant chronic myelogenous leukemia (CML).

    Article Title: Protein Kinase G Type Iα Activity in Human Ovarian Cancer Cells Significantly Contributes to Enhanced Src Activation and DNA Synthesis/Cell Proliferation
    Article Snippet: In vitro Phosphorylation Reaction of Recombinant Human PKG-Iα and Recombinant Human c-Src Interactions between recombinant human PKG-Iα and recombinant human c-Src (both from Calbiochem) were determined using in vitro experiments.

    Immunoprecipitation:

    Article Title: Tyrosine Phosphorylation Regulates the Endocytosis and Surface Expression of GluN3A-Containing NMDA Receptors
    Article Snippet: In vitro phosphorylation was performed by incubating GST-proteins with 9 units of recombinant human c-Src (Millipore Biotechnology) and 5 μCi of [γ- 32 P]ATP (PerkinElmer) for 1 h at 37°C in 30 μl of phosphorylation buffer (50 m m Tris-HCl, pH 7.4, 3 m m MnCl 2 , 10 m m MgCl 2 ) including 83.33 μ m unlabeled ATP (Sigma).

    Article Title: Protein Kinase G Type Iα Activity in Human Ovarian Cancer Cells Significantly Contributes to Enhanced Src Activation and DNA Synthesis/Cell Proliferation
    Article Snippet: Authors' A Cancer Ins Vegas, Nev Gerontolog The Chines 4Reproduc Departmen University Research Major, De Mol Canc D ow naded rom http://aournals.org/m cr/article-p/578/3402560/578 Previously, we showed that basal activity of nitric oxide (NO)/cyclic GMP (cGMP)/protein kinase G (PKG) signaling pathway protects against spontaneous apoptosis and confers resistance to cisplatin-induced apoptosis in human ovarian cancer cells.. The present study determines whether basal PKG kinase activity regulates Src family kinase (SFK) activity and proliferation in these cells.. PKG-Iα was identified as predominant isoform in both OV2008 (cisplatin-sensitive, wild-type p53) and A2780cp (cisplatin-resistant, mutated p53) ovarian cancer cells.

    Article Title: ?5?1 Integrin Engagement Increases Large Conductance, Ca 2+ -activated K + Channel Current and Ca 2+ Sensitivity through c- src -mediated Channel Phosphorylation
    Article Snippet: For in vitro phosphorylation of expressed wild-type and mutant BK channels by human c- src tyrosine kinase, immunoprecipitated BK channels bound to protein A-Sepharose beads were resuspended in 10 m m HEPES, pH 7.6, and 1 m m dithiothreitol and then added to a reaction mixture containing (final) the following: 50 m m HEPES, 125 m m NaCl, 5 m m MgCl 2 , 1 m m dithiothreitol, 0.2 m m Na 3 VO 4 , 4 mg/ml p -nitrophenyl phosphate, and ∼15 units of partially purified recombinant human c- src- (UBI/Millipore), pH 7.6.

    Article Title: Tyrosine Phosphorylation Regulates the Endocytosis and Surface Expression of GluN3A-Containing NMDA Receptors
    Article Snippet: In vitro phosphorylation was performed by incubating GST-proteins with 9 units of recombinant human c-Src (Millipore Biotechnology) and 5 Ci of [ - 32P]ATP (PerkinElmer) for 1 h at 37°C in 30 l of phosphorylation buffer (50 mM Tris-HCl, pH 7.4, 3 mM MnCl2, 10 mM MgCl2) including 83.33 M unlabeled ATP (Sigma).

    Article Title: Trihydrophobin 1 Phosphorylation by c-Src Regulates MAPK/ERK Signaling and Cell Migration
    Article Snippet: Purified GST, GST-TH1 or GST-TH1 mutant (5 μg) was incubated with recombinant human c-Src (5 U, from Millipore, Bedford, MA) in 30 μl kinase buffer (10 mM HEPES, pH 7.5, 150 mM NaCl, 2.5 mM DTT, 0.01% Triton X-100, 10 mM MnCl 2 , 100 μM ATP) for 30 minutes at 30°C.

    Article Title: Characterization of Phospho-(Tyrosine)-Mimetic Calmodulin Mutants
    Article Snippet: Mouse monoclonal anti-phosphotyrosine antibody (clone 4G10, isotype IgG 2bκ ), recombinant c-Src (human) and mouse monoclonal anti-CaM antibody (isotype IgG 1 ) were obtained from Millipore (Billerica, MA).

    Article Title: Cell-Based Proteome Profiling of Potential Dasatinib Targets by Use of Affinity-Based Probes
    Article Snippet: Protein kinases (PKs) play an important role in the development and progression of cancer by regulating cell growth, survival, invasion, metastasis, and angiogenesis.. Dasatinib (BMS-354825), a dual Src/Abl inhibitor, is a promising therapeutic agent with oral bioavailability.. It has been used for the treatment of imatinib-resistant chronic myelogenous leukemia (CML).

    Article Title: Protein Kinase G Type Iα Activity in Human Ovarian Cancer Cells Significantly Contributes to Enhanced Src Activation and DNA Synthesis/Cell Proliferation
    Article Snippet: In vitro Phosphorylation Reaction of Recombinant Human PKG-Iα and Recombinant Human c-Src Interactions between recombinant human PKG-Iα and recombinant human c-Src (both from Calbiochem) were determined using in vitro experiments.



    Similar Products

    95
    Carna Inc human src enzyme
    Human Src Enzyme, supplied by Carna Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+src+enzyme/SRC/us10479780-2133-23-28
    Average 95 stars, based on 1 article reviews
    human src enzyme - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    90
    Upstate Biotechnology Inc 32p-based tyrosine kinase assays with human src enzyme
    32p Based Tyrosine Kinase Assays With Human Src Enzyme, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+src+enzyme/32p+based+tyrosine+kinase+assays+with+human+src+enzyme/pm16513308-86-8-11
    Average 90 stars, based on 1 article reviews
    32p-based tyrosine kinase assays with human src enzyme - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    86
    Thermo Fisher recombinant human src enzyme
    Recombinant Human Src Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+src+enzyme/us06987116-1161-0-7
    Average 86 stars, based on 1 article reviews
    recombinant human src enzyme - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    90
    Upstate Biotechnology Inc human src enzyme
    Human Src Enzyme, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+src+enzyme/human+src+enzyme/pm15313472-64-6-9
    Average 90 stars, based on 1 article reviews
    human src enzyme - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Upstate Biotechnology Inc purified recombinant human c-src enzyme
    Purified Recombinant Human C Src Enzyme, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+src+enzyme/full+length+human+purified+recombinant+pp60c+src/pm12871937-46-0-20
    Average 90 stars, based on 1 article reviews
    purified recombinant human c-src enzyme - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Millipore human recombinant c-src enzyme
    Human Recombinant C Src Enzyme, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+src+enzyme/recombinant+human+c+src/pm09516477-45-2-9
    Average 90 stars, based on 1 article reviews
    human recombinant c-src enzyme - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results