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human serum  (Innovative Research Inc)


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    Structured Review

    Innovative Research Inc human serum
    Human Serum, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 93/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+serum+complement/Pooled+Human+Complement+Serum/us12558398-1207-1-3
    Average 93 stars, based on 50 article reviews
    human serum - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    other:

    Article Title: Arginine regulates the mucoid phenotype of hypervirulent Klebsiella pneumoniae
    Article Snippet: The final elution buffer was exchanged for a storage buffer (10 mM Tris-HCl, 100 mM NaCl, 5% glycerol, pH 8) by dialysis using a 10 K MWCO Slide-A-Lyzer MINI (Thermo Scientific).

    Article Title: Development of a Liposome-Based Serological Assay for SARS-CoV‑2 Variants with Special Emphasis on Coupling Chemistries Required to Maintain Protein Antigenicity
    Article Snippet: Pooled Human Complement Serum (IR45270 and IR46827) was obtained from Innovative Research (Novi, MI, USA).

    Incubation:

    Article Title: Identification of a Kingella kingae factor H binding protein that is the major determinant of serum resistance
    Article Snippet: .. Bacterial samples of 2 x 10 colony forming units (CFU) were pelleted, washed once in PBS, resuspended in PBS, and incubated with varying concentrations of heat inactivated normal human serum (HI-NHS) (pooled human complement serum, Innovative Research, Novi, MI) as a source of FH for 1 h at room temperature (RT) with gentle agitation. ..

    Article Title: Identification of a Kingella kingae factor H binding protein that is the major determinant of serum resistance.
    Article Snippet: .. Bacterial samples of 2 x 108 colony forming units (CFU) were pelleted, washed once in PBS, resuspended in PBS, and incubated with varying concentrations of heat inactivated normal human serum (HI-NHS) (pooled human complement serum, Innovative Research, Novi, Table 2. ..

    Gentle:

    Article Title: Identification of a Kingella kingae factor H binding protein that is the major determinant of serum resistance
    Article Snippet: .. Bacterial samples of 2 x 10 colony forming units (CFU) were pelleted, washed once in PBS, resuspended in PBS, and incubated with varying concentrations of heat inactivated normal human serum (HI-NHS) (pooled human complement serum, Innovative Research, Novi, MI) as a source of FH for 1 h at room temperature (RT) with gentle agitation. ..

    Affinity Chromatography:

    Article Title: Inflammatory disease treatment with complement inhibitors
    Article Snippet: .. Pooled human serum (Innovative Research, Novi, MI) with human complement components was depleted of IgG using Protein-G affinity chromatography. ..

    Sensitivity:

    Article Title: Self-destructing attenuated adjuvant Salmonella strains: design, construction, characterization, and evaluation of safety and efficacy following in ovo inoculation of chicken embryos.
    Article Snippet: .. Pooled human complement serum (PHCS) purchased from Innovative Research (MI, USA) was used to determine the sensitivity of SDAAS strains to complement-mediated killing, as no commercially available chicken sera certified for use in such assays was available. ..



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    Quidel normal human serum complement
    ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human <t>complement.</t> Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.
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    Image Search Results


    ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human complement. Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.

    Journal: Science Advances

    Article Title: Xenopus IgX informs engineering strategies of IgM and IgG hexamers

    doi: 10.1126/sciadv.aea3737

    Figure Lengend Snippet: ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human complement. Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.

    Article Snippet: The RTX-Fcμ or RTX-Fcγ proteins were incubated with equal volumes of cell cultures (~20,000 cells) and 12% normal human serum complement (Quidel) sequentially and then transferred into a 96-microwell plate.

    Techniques: Cell Culture