goat anti human polyclonal reck antibody (Santa Cruz Biotechnology)
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Goat Anti Human Polyclonal Reck Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+reck/RECK+Antibody/pmc07136997-93-4-9
Average 93 stars, based on 45 article reviews
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Membrane:Article Title: CLEC19A overexpression inhibits tumor cell proliferation/migration and promotes apoptosis concomitant suppression of PI3K/AKT/NF-κB signaling pathway in glioblastoma multiforme Article Snippet: Next, membrane blocking was performed at 4 °C for 2 h using 5% skim milk (Sigma, USA) diluted in PBST (Bio basic, Canada). .. Subsequently, the membrane was incubated with the primary antibodies to GFP (Santa Cruz, USA, sc-9996, 1:300), MMP2 (Santa Cruz, USA, sc-10736, 1:300), Article Title: CLEC19A overexpression inhibits tumor cell proliferation/migration and promotes apoptosis concomitant suppression of PI3K/AKT/NF-κB signaling pathway in glioblastoma multiforme. Article Snippet: Next, membrane blocking was performed at 4 °C for 2 h using 5% skim milk (Sigma, USA) diluted in PBST (Bio basic, Canada). .. Subsequently, the membrane was incubated with the primary antibodies to GFP (Santa Cruz, USA, sc-9996, 1:300), MMP2 (Santa Cruz, USA, sc-10736, 1:300), Incubation:Article Title: CLEC19A overexpression inhibits tumor cell proliferation/migration and promotes apoptosis concomitant suppression of PI3K/AKT/NF-κB signaling pathway in glioblastoma multiforme Article Snippet: Next, membrane blocking was performed at 4 °C for 2 h using 5% skim milk (Sigma, USA) diluted in PBST (Bio basic, Canada). .. Subsequently, the membrane was incubated with the primary antibodies to GFP (Santa Cruz, USA, sc-9996, 1:300), MMP2 (Santa Cruz, USA, sc-10736, 1:300), Article Title: Effect of NQO1 Downregulation on the Migration and Invasion of HPV16-Positive Cervical Cancer Cells Article Snippet: For each sample, 200 μg of protein was loaded and separated using a 10% SDS-PAGE gel, followed by transfer to a nitrocellulose membrane (Bio-Rad). .. The membranes were incubated with primary antibodies: NQO1 (ab805588, 1:10,000, Abcam), Article Title: Enhancing RECK Expression Through miR-21 Inhibition: A Promising Strategy for Bladder Carcinoma Control. Article Snippet: Bladder carcinoma (BC) is the tenth most frequent malignancy worldwide, with high morbidity and mortality rates.. Despite recent treatment advances, high-grade BC and muscle-invasive BC present with significant progression and recurrence rates, urging the need for alternative treatments.. The microRNA-21 (miR-21) has superexpression in many malignancies and is associated with cellular invasion and progression. Article Title: CLEC19A overexpression inhibits tumor cell proliferation/migration and promotes apoptosis concomitant suppression of PI3K/AKT/NF-κB signaling pathway in glioblastoma multiforme. Article Snippet: Next, membrane blocking was performed at 4 °C for 2 h using 5% skim milk (Sigma, USA) diluted in PBST (Bio basic, Canada). .. Subsequently, the membrane was incubated with the primary antibodies to GFP (Santa Cruz, USA, sc-9996, 1:300), MMP2 (Santa Cruz, USA, sc-10736, 1:300), Article Title: Effect of NQO1 Downregulation on the Migration and Invasion of HPV16-Positive Cervical Cancer Cells Article Snippet: For each sample, 200 μg of protein was loaded and separated using a 10% SDS-PAGE gel, followed by transfer to a nitrocellulose membrane (Bio-Rad). .. The membranes were incubated with primary antibodies: NQO1 (ab805588, 1:10,000, Abcam), Article Title: FXR agonists INT-787 and OCA increase RECK and inhibit liver steatosis and inflammation in diet-induced ob/ob mouse model of NASH. Article Snippet: Immunostaining was revealed with BIO-RAD Chemidoc XRS+, visualized using the ECL Clarity (BIO-RAD), and intensity quantification performed by Image Lab SoftwareTM6.0.1 (Bio-Rad). .. Acetone-fixed, 4 μm thick, cut frozen liver sections, were treated for 8 mins with UltraVision ProteinBlock (ThermoFisher Scientific), and then immunostained by overnight incubation at 4°C with rabbit polyclonal anti-wide spectrum screening cytokeratins (pCK, Agilent) and Immunoprecipitation:Article Title: RECK/GPR124 ‐driven WNT signaling in pancreatic and gastric cancer cells Article Snippet: A total of 0.8 × 10 5 cells were seeded onto six‐well plates and cultured after several days and harvested using magnesium‐containing lysis buffer (MLB) supplemented with 0.5 mM NaF (#31418‐45, Nacalai Tesque), 100 μM Na VO 4 (#190‐09751, FUJIFILM) and 1X protease inhibitor (#25955‐11, Nacalai Tesque). .. RECK was immunoprecipitated with mouse Article Title: RECK/GPR124-driven WNT signaling in pancreatic and gastric cancer cells. Article Snippet: RECK has been identified as a cDNA clone contained in an Okayama– Berg cDNA expression library driven from MRC5 untransformed human fibroblasts mRNAs.. According to the “flat reversion cloning method,”1,2 aiming to isolate a gene whose overexpression causes an untransformed celllike flat phenotype in highly transformed NIH3T3 cells which have been doubly infected with retrovirus harboring oncogenic KRASG12V, we isolated a cDNA from a “flat revertant” clone that appeared after drug selection.. The initially isolated cDNA clone was named RECK, but it lacked the 5′end containing the initiation codon; thus, there was no reason for calling this gene a “KRAS transformation suppressor.” However, RECK gene expression appeared to be suppressed by a number of oncogenic gene products including Ras, Fos, Myc, Src, Fms, Fes, and Mos, and moreover, universally underexpressed in cancer tissues compared with normal tissues.3–5 Low expression of RECK in cancers overall predicts poor prognosis.6–16 Received: 23 April 2024 | Revised: 5 June 2024 | Accepted: 16 June 2024 DOI: 10.1111/cas.16258 Immunofluorescence:Article Title: Enhancing RECK Expression Through miR-21 Inhibition: A Promising Strategy for Bladder Carcinoma Control. Article Snippet: Bladder carcinoma (BC) is the tenth most frequent malignancy worldwide, with high morbidity and mortality rates.. Despite recent treatment advances, high-grade BC and muscle-invasive BC present with significant progression and recurrence rates, urging the need for alternative treatments.. The microRNA-21 (miR-21) has superexpression in many malignancies and is associated with cellular invasion and progression. Blocking Assay:Article Title: Enhancing RECK Expression Through miR-21 Inhibition: A Promising Strategy for Bladder Carcinoma Control. Article Snippet: Bladder carcinoma (BC) is the tenth most frequent malignancy worldwide, with high morbidity and mortality rates.. Despite recent treatment advances, high-grade BC and muscle-invasive BC present with significant progression and recurrence rates, urging the need for alternative treatments.. The microRNA-21 (miR-21) has superexpression in many malignancies and is associated with cellular invasion and progression. |
