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goat anti human polyclonal reck antibody  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology goat anti human polyclonal reck antibody
    Goat Anti Human Polyclonal Reck Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+reck/RECK+Antibody/pmc07136997-93-4-9
    Average 93 stars, based on 45 article reviews
    goat anti human polyclonal reck antibody - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Membrane:

    Article Title: CLEC19A overexpression inhibits tumor cell proliferation/migration and promotes apoptosis concomitant suppression of PI3K/AKT/NF-κB signaling pathway in glioblastoma multiforme
    Article Snippet: Next, membrane blocking was performed at 4 °C for 2 h using 5% skim milk (Sigma, USA) diluted in PBST (Bio basic, Canada). .. Subsequently, the membrane was incubated with the primary antibodies to GFP (Santa Cruz, USA, sc-9996, 1:300), MMP2 (Santa Cruz, USA, sc-10736, 1:300), RECK (Santa Cruz, USA, sc-373929, 1:300), BAX (Santa Cruz, USA, sc-7480, 1:300), BCL2 (Santa Cruz, USA, sc-492, 1:300), IκB-α (Santa Cruz, USA, sc-1643, 1:300), TIMP3 (Santa Cruz, USA, sc-373839, 1:300), PI3K (Santa Cruz, USA, sc-67306, 1:300), and β-Actin (Santa Cruz, USA, sc-47778, 1:300) overnight at 4 °C. .. The anti-mouse IgG-Horse Radish Peroxidase (HRP) (Santa Cruz, USA, sc-516102, 1:1000) or anti-rabbit IgG-HRP (Santa Cruz, USA, sc-2357, 1:1000) secondary antibodies were diluted according to the manufacturer’s instructions and was incubated with the membrane for 1 h after three times washes with PBST.

    Article Title: CLEC19A overexpression inhibits tumor cell proliferation/migration and promotes apoptosis concomitant suppression of PI3K/AKT/NF-κB signaling pathway in glioblastoma multiforme.
    Article Snippet: Next, membrane blocking was performed at 4 °C for 2 h using 5% skim milk (Sigma, USA) diluted in PBST (Bio basic, Canada). .. Subsequently, the membrane was incubated with the primary antibodies to GFP (Santa Cruz, USA, sc-9996, 1:300), MMP2 (Santa Cruz, USA, sc-10736, 1:300), RECK (Santa Cruz, USA, sc-373929, 1:300), BAX (Santa Cruz, USA, sc-7480, 1:300), BCL2 (Santa Cruz, USA, sc-492, 1:300), IκB-α (Santa Cruz, USA, sc-1643, 1:300), TIMP3 (Santa Cruz, USA, sc-373839, 1:300), PI3K (Santa Cruz, USA, sc-67306, 1:300), and β-Actin (Santa Cruz, USA, sc-47778, 1:300) overnight at 4 °C. .. The antimouse IgG-Horse Radish Peroxidase (HRP) (Santa Cruz, USA, sc-516102, 1:1000) or anti-rabbit IgG-HRP (Santa Cruz, USA, sc-2357, 1:1000) secondary antibodies were diluted according to the manufacturer’s instructions and was incubated with the membrane for 1 h after three times washes with PBST.

    Incubation:

    Article Title: CLEC19A overexpression inhibits tumor cell proliferation/migration and promotes apoptosis concomitant suppression of PI3K/AKT/NF-κB signaling pathway in glioblastoma multiforme
    Article Snippet: Next, membrane blocking was performed at 4 °C for 2 h using 5% skim milk (Sigma, USA) diluted in PBST (Bio basic, Canada). .. Subsequently, the membrane was incubated with the primary antibodies to GFP (Santa Cruz, USA, sc-9996, 1:300), MMP2 (Santa Cruz, USA, sc-10736, 1:300), RECK (Santa Cruz, USA, sc-373929, 1:300), BAX (Santa Cruz, USA, sc-7480, 1:300), BCL2 (Santa Cruz, USA, sc-492, 1:300), IκB-α (Santa Cruz, USA, sc-1643, 1:300), TIMP3 (Santa Cruz, USA, sc-373839, 1:300), PI3K (Santa Cruz, USA, sc-67306, 1:300), and β-Actin (Santa Cruz, USA, sc-47778, 1:300) overnight at 4 °C. .. The anti-mouse IgG-Horse Radish Peroxidase (HRP) (Santa Cruz, USA, sc-516102, 1:1000) or anti-rabbit IgG-HRP (Santa Cruz, USA, sc-2357, 1:1000) secondary antibodies were diluted according to the manufacturer’s instructions and was incubated with the membrane for 1 h after three times washes with PBST.

    Article Title: Effect of NQO1 Downregulation on the Migration and Invasion of HPV16-Positive Cervical Cancer Cells
    Article Snippet: For each sample, 200 μg of protein was loaded and separated using a 10% SDS-PAGE gel, followed by transfer to a nitrocellulose membrane (Bio-Rad). .. The membranes were incubated with primary antibodies: NQO1 (ab805588, 1:10,000, Abcam), RECK (sc-373929, 1:1,000, Santa Cruz Biotechnology), PI3K (p110, 4255, 1:1,000, Cell Signaling Technology), p-PI3K (p-p85, 4228, 1:1,000, Cell Signaling Technology), p53 (9282, 1:1,000, Cell Signaling Technology), and GAPDH (sc-47724, 1:200, Santa Cruz Biotechnology). .. Following primary antibody incubation, the membranes were incubated with secondary HRP-conjugated antibodies: rabbit anti-mouse (ab205719) and anti-rabbit IgG (ab205718) antibodies (Abcam).

    Article Title: Enhancing RECK Expression Through miR-21 Inhibition: A Promising Strategy for Bladder Carcinoma Control.
    Article Snippet: Bladder carcinoma (BC) is the tenth most frequent malignancy worldwide, with high morbidity and mortality rates.. Despite recent treatment advances, high-grade BC and muscle-invasive BC present with significant progression and recurrence rates, urging the need for alternative treatments.. The microRNA-21 (miR-21) has superexpression in many malignancies and is associated with cellular invasion and progression.

    Article Title: CLEC19A overexpression inhibits tumor cell proliferation/migration and promotes apoptosis concomitant suppression of PI3K/AKT/NF-κB signaling pathway in glioblastoma multiforme.
    Article Snippet: Next, membrane blocking was performed at 4 °C for 2 h using 5% skim milk (Sigma, USA) diluted in PBST (Bio basic, Canada). .. Subsequently, the membrane was incubated with the primary antibodies to GFP (Santa Cruz, USA, sc-9996, 1:300), MMP2 (Santa Cruz, USA, sc-10736, 1:300), RECK (Santa Cruz, USA, sc-373929, 1:300), BAX (Santa Cruz, USA, sc-7480, 1:300), BCL2 (Santa Cruz, USA, sc-492, 1:300), IκB-α (Santa Cruz, USA, sc-1643, 1:300), TIMP3 (Santa Cruz, USA, sc-373839, 1:300), PI3K (Santa Cruz, USA, sc-67306, 1:300), and β-Actin (Santa Cruz, USA, sc-47778, 1:300) overnight at 4 °C. .. The antimouse IgG-Horse Radish Peroxidase (HRP) (Santa Cruz, USA, sc-516102, 1:1000) or anti-rabbit IgG-HRP (Santa Cruz, USA, sc-2357, 1:1000) secondary antibodies were diluted according to the manufacturer’s instructions and was incubated with the membrane for 1 h after three times washes with PBST.

    Article Title: Effect of NQO1 Downregulation on the Migration and Invasion of HPV16-Positive Cervical Cancer Cells
    Article Snippet: For each sample, 200 μg of protein was loaded and separated using a 10% SDS-PAGE gel, followed by transfer to a nitrocellulose membrane (Bio-Rad). .. The membranes were incubated with primary antibodies: NQO1 (ab805588, 1:10,000, Abcam), RECK (sc-373929, 1:1,000, Santa Cruz Biotechnology), PI3K (p110, 4255, 1:1,000, Cell Signaling Technology), p-PI3K (p-p85, 4228, 1:1,000, Cell Signaling Technology), p53 (9282, 1:1,000, Cell Signaling Technology), and GAPDH (sc-47724, 1:200, Santa Cruz Biotechnology). .. Following primary antibody incubation, the membranes were incubated with secondary HRP-conjugated antibodies: rabbit anti-mouse (ab205719) and anti-rabbit IgG (ab205718) antibodies (Abcam).

    Article Title: FXR agonists INT-787 and OCA increase RECK and inhibit liver steatosis and inflammation in diet-induced ob/ob mouse model of NASH.
    Article Snippet: Immunostaining was revealed with BIO-RAD Chemidoc XRS+, visualized using the ECL Clarity (BIO-RAD), and intensity quantification performed by Image Lab SoftwareTM6.0.1 (Bio-Rad). .. Acetone-fixed, 4 μm thick, cut frozen liver sections, were treated for 8 mins with UltraVision ProteinBlock (ThermoFisher Scientific), and then immunostained by overnight incubation at 4°C with rabbit polyclonal anti-wide spectrum screening cytokeratins (pCK, Agilent) and anti-RECK (Santa Cruz Biotechnology). .. Following incubation, slides were rinsed, incubated with the appropriate secondary antibody (Alexa fluor 488 or 594, ThermoFisher Scientific) and then mounted with ProLong Gold with DAPI (ThermoFisher Scientific) antifade mounting media.

    Immunoprecipitation:

    Article Title: RECK/GPR124 ‐driven WNT signaling in pancreatic and gastric cancer cells
    Article Snippet: A total of 0.8 × 10 5 cells were seeded onto six‐well plates and cultured after several days and harvested using magnesium‐containing lysis buffer (MLB) supplemented with 0.5 mM NaF (#31418‐45, Nacalai Tesque), 100 μM Na VO 4 (#190‐09751, FUJIFILM) and 1X protease inhibitor (#25955‐11, Nacalai Tesque). .. RECK was immunoprecipitated with mouse anti‐RECK antibody (5B11D12) and Myc‐GPR124 with Myc antibody (#9E10, sc‐40; Santa Cruz). .. RECK was immunoprecipitated with mouse anti‐RECK antibody (5B11D12) and Myc‐GPR124 with Myc antibody (#9E10, sc‐40; Santa Cruz).

    Article Title: RECK/GPR124-driven WNT signaling in pancreatic and gastric cancer cells.
    Article Snippet: RECK has been identified as a cDNA clone contained in an Okayama– Berg cDNA expression library driven from MRC5 untransformed human fibroblasts mRNAs.. According to the “flat reversion cloning method,”1,2 aiming to isolate a gene whose overexpression causes an untransformed celllike flat phenotype in highly transformed NIH3T3 cells which have been doubly infected with retrovirus harboring oncogenic KRASG12V, we isolated a cDNA from a “flat revertant” clone that appeared after drug selection.. The initially isolated cDNA clone was named RECK, but it lacked the 5′end containing the initiation codon; thus, there was no reason for calling this gene a “KRAS transformation suppressor.” However, RECK gene expression appeared to be suppressed by a number of oncogenic gene products including Ras, Fos, Myc, Src, Fms, Fes, and Mos, and moreover, universally underexpressed in cancer tissues compared with normal tissues.3–5 Low expression of RECK in cancers overall predicts poor prognosis.6–16 Received: 23 April 2024 | Revised: 5 June 2024 | Accepted: 16 June 2024 DOI: 10.1111/cas.16258

    Immunofluorescence:

    Article Title: Enhancing RECK Expression Through miR-21 Inhibition: A Promising Strategy for Bladder Carcinoma Control.
    Article Snippet: Bladder carcinoma (BC) is the tenth most frequent malignancy worldwide, with high morbidity and mortality rates.. Despite recent treatment advances, high-grade BC and muscle-invasive BC present with significant progression and recurrence rates, urging the need for alternative treatments.. The microRNA-21 (miR-21) has superexpression in many malignancies and is associated with cellular invasion and progression.

    Blocking Assay:

    Article Title: Enhancing RECK Expression Through miR-21 Inhibition: A Promising Strategy for Bladder Carcinoma Control.
    Article Snippet: Bladder carcinoma (BC) is the tenth most frequent malignancy worldwide, with high morbidity and mortality rates.. Despite recent treatment advances, high-grade BC and muscle-invasive BC present with significant progression and recurrence rates, urging the need for alternative treatments.. The microRNA-21 (miR-21) has superexpression in many malignancies and is associated with cellular invasion and progression.



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    Goat Anti Human Polyclonal Reck Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+reck/RECK+Antibody/pmc07136997-93-4-9
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    Targeting TRAF3IP2 or RECK overexpression blunts IL-18-induced ASMC proliferation and migration. ( A – E ) Silencing TRAF3IP2 inhibits IL-18-induced ASMC proliferation ( B ) and migration ( C ). ASMC were transduced with adenovirus-expressing shRNA targeting human TRAF3IP2 (moi 10 for 48 h), made quiescent, and then exposed to IL-18 (10 ng/mL; experimental design in ( A )). ASMC proliferation was assessed after 48 h of IL-18 addition using the CyQUANT Cell proliferation assay ( B ), and migration after 18 h using Boyden chamber assay ( C ). ASMCs migrating to the lower surface of the membrane were counted in 10 different fields and summarized as mean ± SEM. ( B , C ) * p < at least 0.01 vs. Untreated; † p < 0.01 vs. IL-18 or IL-18+GFP (n = 6). Knockdown of TRAF3IP2 was confirmed by RT-qPCR using a TaqMan™ probe ( D ) and Western blotting ( E ). ( D , E ) * p < 0.01 vs. untreated (n = 3). ( F , G ) Dose-dependent effects of Ad.RECK on RECK expression (experimental design in ( F )). Induction of RECK following adenoviral transduction was confirmed by Western blotting with tubulin serving as an internal control ( G ). ( H – K ) Forced expression of RECK inhibits IL-18-stimulated ASMC proliferation and migration. ASMCs were transduced with adenovirus-expressing human RECK cDNA (moi 10 for 24 h), made quiescent, and then treated with IL-18 (experimental design in ( H )) and analyzed for proliferation ( I ) and migration ( J ) as in ( B , C ). ( C , J ) The insets show representative images of Matrigel™ Transwell invasion. Scale bar: 20 μM. ( E , G , K ) While a representative immunoblot is shown, the intensities of immunoreactive bands from three ( E ), four ( G ) and three ( K ) independent experiments were semiquantified by densitometry and are summarized on the right. ( I ) * p < at least 0.01 vs. Untreated; † p < 0.01 vs. IL-18 or IL-18+GFP (n = 6); ( J ) * p < at least 0.01 vs. Untreated; † p < 0.01 vs. IL-18 or IL-18+eGFP (n = 4); ( K ) * p < 0.01 vs. Untreated; † p < 0.05 vs. IL-18 or IL-18+eGFP (n = 3).

    Journal: Cells

    Article Title: EF24, a Curcumin Analog, Reverses Interleukin-18-Induced miR-30a or miR-342-Dependent TRAF3IP2 Expression, RECK Suppression, and the Proinflammatory Phenotype of Human Aortic Smooth Muscle Cells

    doi: 10.3390/cells13201673

    Figure Lengend Snippet: Targeting TRAF3IP2 or RECK overexpression blunts IL-18-induced ASMC proliferation and migration. ( A – E ) Silencing TRAF3IP2 inhibits IL-18-induced ASMC proliferation ( B ) and migration ( C ). ASMC were transduced with adenovirus-expressing shRNA targeting human TRAF3IP2 (moi 10 for 48 h), made quiescent, and then exposed to IL-18 (10 ng/mL; experimental design in ( A )). ASMC proliferation was assessed after 48 h of IL-18 addition using the CyQUANT Cell proliferation assay ( B ), and migration after 18 h using Boyden chamber assay ( C ). ASMCs migrating to the lower surface of the membrane were counted in 10 different fields and summarized as mean ± SEM. ( B , C ) * p < at least 0.01 vs. Untreated; † p < 0.01 vs. IL-18 or IL-18+GFP (n = 6). Knockdown of TRAF3IP2 was confirmed by RT-qPCR using a TaqMan™ probe ( D ) and Western blotting ( E ). ( D , E ) * p < 0.01 vs. untreated (n = 3). ( F , G ) Dose-dependent effects of Ad.RECK on RECK expression (experimental design in ( F )). Induction of RECK following adenoviral transduction was confirmed by Western blotting with tubulin serving as an internal control ( G ). ( H – K ) Forced expression of RECK inhibits IL-18-stimulated ASMC proliferation and migration. ASMCs were transduced with adenovirus-expressing human RECK cDNA (moi 10 for 24 h), made quiescent, and then treated with IL-18 (experimental design in ( H )) and analyzed for proliferation ( I ) and migration ( J ) as in ( B , C ). ( C , J ) The insets show representative images of Matrigel™ Transwell invasion. Scale bar: 20 μM. ( E , G , K ) While a representative immunoblot is shown, the intensities of immunoreactive bands from three ( E ), four ( G ) and three ( K ) independent experiments were semiquantified by densitometry and are summarized on the right. ( I ) * p < at least 0.01 vs. Untreated; † p < 0.01 vs. IL-18 or IL-18+GFP (n = 6); ( J ) * p < at least 0.01 vs. Untreated; † p < 0.01 vs. IL-18 or IL-18+eGFP (n = 4); ( K ) * p < 0.01 vs. Untreated; † p < 0.05 vs. IL-18 or IL-18+eGFP (n = 3).

    Article Snippet: Adenoviral vector-expressing human RECK cDNA (GenBank accession # NM_021111) was also custom-generated at Vector Biolabs.

    Techniques: Over Expression, Migration, Transduction, Expressing, shRNA, CyQUANT Assay, Proliferation Assay, Boyden Chamber Assay, Membrane, Knockdown, Quantitative RT-PCR, Western Blot, Control

    Ectopic expression of RECK blunts IL-18-induced inhibition in the expression of SMC markers and the induction of proinflammatory phenotype markers. ( A – F ) Forced expression of RECK restores IL-18-induced suppression of SMC markers and inhibits the induction of proinflammatory phenotype markers. ASMCs were transduced with adenovirus-expressing human RECK cDNA (moi10 for 24 h), made quiescent, and then treated with IL-18 at 10 ng/mL for 48 h (experimental design in ( A )). The expression levels of SMC markers ACTA2 ( B , C ) and MYH11 ( D , E ) were analyzed by RT-qPCR ( B , D ) and Western blotting ( C , E ). The proinflammatory phenotype markers Galectin 3, Olr1, VCAM, CCL2, IL-6, IL-8, and TNF-α were analyzed by RT-qPCR using TaqMan™ probes ( F ). ( C , E ) While a representative immunoblot is shown, the intensities of immunoreactive bands from three independent experiments were semiquantified by densitometry and are summarized on the right. ( B , D , F ) * p < at least 0.01 vs. Untreated; † p < 0.01 vs. IL-18 or IL-18+eGFP (n = 5). ( C , E ) * p < 0.05 vs. Untreated; † p < 0.05 vs. IL-18 or IL-18+eGFP (n = 3).

    Journal: Cells

    Article Title: EF24, a Curcumin Analog, Reverses Interleukin-18-Induced miR-30a or miR-342-Dependent TRAF3IP2 Expression, RECK Suppression, and the Proinflammatory Phenotype of Human Aortic Smooth Muscle Cells

    doi: 10.3390/cells13201673

    Figure Lengend Snippet: Ectopic expression of RECK blunts IL-18-induced inhibition in the expression of SMC markers and the induction of proinflammatory phenotype markers. ( A – F ) Forced expression of RECK restores IL-18-induced suppression of SMC markers and inhibits the induction of proinflammatory phenotype markers. ASMCs were transduced with adenovirus-expressing human RECK cDNA (moi10 for 24 h), made quiescent, and then treated with IL-18 at 10 ng/mL for 48 h (experimental design in ( A )). The expression levels of SMC markers ACTA2 ( B , C ) and MYH11 ( D , E ) were analyzed by RT-qPCR ( B , D ) and Western blotting ( C , E ). The proinflammatory phenotype markers Galectin 3, Olr1, VCAM, CCL2, IL-6, IL-8, and TNF-α were analyzed by RT-qPCR using TaqMan™ probes ( F ). ( C , E ) While a representative immunoblot is shown, the intensities of immunoreactive bands from three independent experiments were semiquantified by densitometry and are summarized on the right. ( B , D , F ) * p < at least 0.01 vs. Untreated; † p < 0.01 vs. IL-18 or IL-18+eGFP (n = 5). ( C , E ) * p < 0.05 vs. Untreated; † p < 0.05 vs. IL-18 or IL-18+eGFP (n = 3).

    Article Snippet: Adenoviral vector-expressing human RECK cDNA (GenBank accession # NM_021111) was also custom-generated at Vector Biolabs.

    Techniques: Expressing, Inhibition, Transduction, Quantitative RT-PCR, Western Blot