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neutrophil isolation kit  (Beijing Solarbio Science)


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    Beijing Solarbio Science neutrophil isolation kit
    Ginseng and Salvia miltiorrhiza modulated the <t>neutrophil-associated</t> TIME in the lungs. (A) Experimental setup. The mice were constructed as experimental metastasis models after a period of acclimatization. The mice were prepared as experimental metastasis models following an acclimatization period. Ginseng and Salvia miltiorrhiza extracts were administered daily to the relevant groups according to the schematic diagram, and lung tissues were collected on day 28. (B) Representative bioluminescence images of metastasis in the intravenously implanted LLC mice. (C) Representative H&E images of the tumor area in the lungs of the mice (scale bar: 50 μm). (D) Quantitative analysis of the lung tumor area ( n = 3). (E) Quantitative analysis of the number of lung metastatic nodules in tumor-bearing mice( n = 15). (F) Metastasis rates of LLC tumor-bearing mice ( n = 15). (G) GOBP analysis of intersecting genes in the control, model and G + S groups. (H) GSEA of genes related to neutrophil migration. (I) Representative images of immunohistochemical staining and quantitative analysis of Ly6G in the lungs ( n = 3; scale bar: 50 μm). (J) Representative images of immunofluorescence staining and quantitative analysis of Ly6G in the lungs ( n = 3; scale bar: 100 μm). All the data are presented as mean ± SEM. #### p < 0.0001 vs. the control group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs the model group.
    Neutrophil Isolation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 95/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+phosphorylated+rtk+array+kit/Human+Peripheral+Blood+Neutrophil+Isolation+Solution+Kit/pmc12789768-94-13-17
    Average 95 stars, based on 116 article reviews
    neutrophil isolation kit - by Bioz Stars, 2026-11
    95/100 stars

    Images

    1) Product Images from "Synergistic potentiation of the anti-metastatic effect of a Ginseng - Salvia miltiorrhiza herbal pair and its biological ingredients via the suppression of CD62E-dependent neutrophil infiltration and NETformation"

    Article Title: Synergistic potentiation of the anti-metastatic effect of a Ginseng - Salvia miltiorrhiza herbal pair and its biological ingredients via the suppression of CD62E-dependent neutrophil infiltration and NETformation

    Journal: Journal of Advanced Research

    doi: 10.1016/j.jare.2024.10.036

    Ginseng and Salvia miltiorrhiza modulated the neutrophil-associated TIME in the lungs. (A) Experimental setup. The mice were constructed as experimental metastasis models after a period of acclimatization. The mice were prepared as experimental metastasis models following an acclimatization period. Ginseng and Salvia miltiorrhiza extracts were administered daily to the relevant groups according to the schematic diagram, and lung tissues were collected on day 28. (B) Representative bioluminescence images of metastasis in the intravenously implanted LLC mice. (C) Representative H&E images of the tumor area in the lungs of the mice (scale bar: 50 μm). (D) Quantitative analysis of the lung tumor area ( n = 3). (E) Quantitative analysis of the number of lung metastatic nodules in tumor-bearing mice( n = 15). (F) Metastasis rates of LLC tumor-bearing mice ( n = 15). (G) GOBP analysis of intersecting genes in the control, model and G + S groups. (H) GSEA of genes related to neutrophil migration. (I) Representative images of immunohistochemical staining and quantitative analysis of Ly6G in the lungs ( n = 3; scale bar: 50 μm). (J) Representative images of immunofluorescence staining and quantitative analysis of Ly6G in the lungs ( n = 3; scale bar: 100 μm). All the data are presented as mean ± SEM. #### p < 0.0001 vs. the control group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs the model group.
    Figure Legend Snippet: Ginseng and Salvia miltiorrhiza modulated the neutrophil-associated TIME in the lungs. (A) Experimental setup. The mice were constructed as experimental metastasis models after a period of acclimatization. The mice were prepared as experimental metastasis models following an acclimatization period. Ginseng and Salvia miltiorrhiza extracts were administered daily to the relevant groups according to the schematic diagram, and lung tissues were collected on day 28. (B) Representative bioluminescence images of metastasis in the intravenously implanted LLC mice. (C) Representative H&E images of the tumor area in the lungs of the mice (scale bar: 50 μm). (D) Quantitative analysis of the lung tumor area ( n = 3). (E) Quantitative analysis of the number of lung metastatic nodules in tumor-bearing mice( n = 15). (F) Metastasis rates of LLC tumor-bearing mice ( n = 15). (G) GOBP analysis of intersecting genes in the control, model and G + S groups. (H) GSEA of genes related to neutrophil migration. (I) Representative images of immunohistochemical staining and quantitative analysis of Ly6G in the lungs ( n = 3; scale bar: 50 μm). (J) Representative images of immunofluorescence staining and quantitative analysis of Ly6G in the lungs ( n = 3; scale bar: 100 μm). All the data are presented as mean ± SEM. #### p < 0.0001 vs. the control group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs the model group.

    Techniques Used: Construct, Control, Migration, Immunohistochemical staining, Staining, Immunofluorescence

    CPT inhibited CD62E–mediated neutrophil adhesion and recruitment to metastases. (A) Representative images and quantification of HL–60 cell adhesion to HUVECs (scale bar: 200 μm). (B) Schematic diagram of the parallel plate flow chamber combined with the microscope. (C) Number of neutrophils firmly adhered to HUVECs. (D) Representative immunofluorescence images and quantification of CD62E in the lungs ( n = 3; scale bar: 50 μm). (E) Representative western blot images of CD62E levels in the lungs and quantitative data ( n = 3). GAPDH was used as a loading control. (F) The mRNA expression levels of TNF–α, ICAM1, and VCAM1 in lung tissues were determined by qRT–PCR ( n = 3). (G) The correlation between CD62E expression and neutrophil infiltration in LUAD and LUSC was analyzed via TIMER. (H) Representative images and quantification of Ly6G + neutrophils in lungs detected by flow cytometry ( n = 4). (I) Representative immunofluorescence images and quantification of Ly6G + neutrophils in the lungs (n = 3; scale bar: 50 μm). All the data are presented as mean ± SEM. ## p < 0.01, ### p < 0.001, #### p < 0.0001 vs. the control group. * p < 0.05, ** p < 0.01, **** p < 0.0001 vs. the model group.
    Figure Legend Snippet: CPT inhibited CD62E–mediated neutrophil adhesion and recruitment to metastases. (A) Representative images and quantification of HL–60 cell adhesion to HUVECs (scale bar: 200 μm). (B) Schematic diagram of the parallel plate flow chamber combined with the microscope. (C) Number of neutrophils firmly adhered to HUVECs. (D) Representative immunofluorescence images and quantification of CD62E in the lungs ( n = 3; scale bar: 50 μm). (E) Representative western blot images of CD62E levels in the lungs and quantitative data ( n = 3). GAPDH was used as a loading control. (F) The mRNA expression levels of TNF–α, ICAM1, and VCAM1 in lung tissues were determined by qRT–PCR ( n = 3). (G) The correlation between CD62E expression and neutrophil infiltration in LUAD and LUSC was analyzed via TIMER. (H) Representative images and quantification of Ly6G + neutrophils in lungs detected by flow cytometry ( n = 4). (I) Representative immunofluorescence images and quantification of Ly6G + neutrophils in the lungs (n = 3; scale bar: 50 μm). All the data are presented as mean ± SEM. ## p < 0.01, ### p < 0.001, #### p < 0.0001 vs. the control group. * p < 0.05, ** p < 0.01, **** p < 0.0001 vs. the model group.

    Techniques Used: Microscopy, Immunofluorescence, Western Blot, Control, Expressing, Quantitative RT-PCR, Flow Cytometry

    Neutrophil RNA sequencing analysis and correlation analysis of NETs and lung cancer. (A) Schematic diagram of neutrophil isolation and RNA sequencing from mouse lung tissues. (B-C) Heatmap analysis of neutrophil-related genes and KEGG analysis of altered genes. (D) Results of partial GOBP analysis of intersecting genes in the control, model and G + S groups. (E) The expression of HIST3H3 in different cancers and adjacent normal tissues was analyzed via TIMER. (F–H) The correlation between copy number alterations in HIST3H3, ELANE, and MPO and neutrophil infiltration was analyzed via TIMER. (I) The correlations between cumulative survival and HIST3H3, ELANE, MPO and neutrophil infiltration in lung cancer patients were analyzed via TIMER. A two-sided Wilcoxon rank-sum test was used. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. the control group.
    Figure Legend Snippet: Neutrophil RNA sequencing analysis and correlation analysis of NETs and lung cancer. (A) Schematic diagram of neutrophil isolation and RNA sequencing from mouse lung tissues. (B-C) Heatmap analysis of neutrophil-related genes and KEGG analysis of altered genes. (D) Results of partial GOBP analysis of intersecting genes in the control, model and G + S groups. (E) The expression of HIST3H3 in different cancers and adjacent normal tissues was analyzed via TIMER. (F–H) The correlation between copy number alterations in HIST3H3, ELANE, and MPO and neutrophil infiltration was analyzed via TIMER. (I) The correlations between cumulative survival and HIST3H3, ELANE, MPO and neutrophil infiltration in lung cancer patients were analyzed via TIMER. A two-sided Wilcoxon rank-sum test was used. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. the control group.

    Techniques Used: RNA Sequencing, Isolation, Control, Expressing

    Ginseng and Salvia miltiorrhiza reduced the generation of NETs in lung tissues, and effective substances from ginseng were screened. (A) Representative immunohistochemical images of MPO expression in the lungs (scale bar: 100 μm). (B) Representative immunofluorescence images of H3cit and NE in the lungs (scale bar: 100 μm). (C–E) Quantitative analysis of MPO, H3cit and NE expression in the lungs ( n = 3). (F) Neutrophil MPO release was detected by ELISA. The chemical structure of Rg1 is shown on the right. (G) Representative images and quantification of extracellular DNA release after Sytox™ Green staining (scale bar: 50 μm). All the data are presented as mean ± SEM. ####p < 0.0001 vs. the control group. * p < 0.05, ** p < 0.01, ***p < 0.001, **** p < 0.0001 vs. the model group.
    Figure Legend Snippet: Ginseng and Salvia miltiorrhiza reduced the generation of NETs in lung tissues, and effective substances from ginseng were screened. (A) Representative immunohistochemical images of MPO expression in the lungs (scale bar: 100 μm). (B) Representative immunofluorescence images of H3cit and NE in the lungs (scale bar: 100 μm). (C–E) Quantitative analysis of MPO, H3cit and NE expression in the lungs ( n = 3). (F) Neutrophil MPO release was detected by ELISA. The chemical structure of Rg1 is shown on the right. (G) Representative images and quantification of extracellular DNA release after Sytox™ Green staining (scale bar: 50 μm). All the data are presented as mean ± SEM. ####p < 0.0001 vs. the control group. * p < 0.05, ** p < 0.01, ***p < 0.001, **** p < 0.0001 vs. the model group.

    Techniques Used: Immunohistochemical staining, Expressing, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Staining, Control

    Rg1 reduced NET formation by inhibiting ROS production and ERK1/2 and MAPK phosphorylation. (A) Representative immunofluorescence images of the NET components H3cit and NE in neutrophils ( n = 3; scale bar: 50 μm). (B–C) Representative western blot images of H3cit expression in neutrophils and quantitative data. GAPDH was used as a loading control ( n = 3). (D-E) ROS production in dHL-60 cells (D) and neutrophils (E) was assessed by flow cytometry. The median fluorescence intensity of ROS was used for quantification. (F-G) Representative western blot images and quantification of p-ERK1/2, ERK1/2, p-MAPK and MAPK expression in dHL-60 cells (F) and neutrophils (G). GAPDH was used as a loading control. All the data are presented as mean ± SEM. # p < 0.05, ### p < 0.001, #### p < 0.0001 vs. the control group. * p < 0.05, ** p < 0.01, **** p < 0.0001 vs. the model group.
    Figure Legend Snippet: Rg1 reduced NET formation by inhibiting ROS production and ERK1/2 and MAPK phosphorylation. (A) Representative immunofluorescence images of the NET components H3cit and NE in neutrophils ( n = 3; scale bar: 50 μm). (B–C) Representative western blot images of H3cit expression in neutrophils and quantitative data. GAPDH was used as a loading control ( n = 3). (D-E) ROS production in dHL-60 cells (D) and neutrophils (E) was assessed by flow cytometry. The median fluorescence intensity of ROS was used for quantification. (F-G) Representative western blot images and quantification of p-ERK1/2, ERK1/2, p-MAPK and MAPK expression in dHL-60 cells (F) and neutrophils (G). GAPDH was used as a loading control. All the data are presented as mean ± SEM. # p < 0.05, ### p < 0.001, #### p < 0.0001 vs. the control group. * p < 0.05, ** p < 0.01, **** p < 0.0001 vs. the model group.

    Techniques Used: Phospho-proteomics, Immunofluorescence, Western Blot, Expressing, Control, Flow Cytometry, Fluorescence

    Rg1 reversed NET-induced invasion, EMT, and adhesion of LLC cells and increased vascular permeability. (A) LLC cell proliferation was detected via a CCK8 assay. (B) Schematic diagram of NET isolation and coculture with LLC cells in the transwell system. (C) Images of the transwell invasion assay (scale bar, 200 μm). The number of infiltrated cells and the OD values were used for quantification. (D-E) Representative immunofluorescence images and quantification of MMP9 in the lungs (n = 3, scale bar 50 μm). (F) Representative immunofluorescence images of E-cadherin and N-cadherin in LLC cells treated with or without Rg1 or NETs. (G-I) Representative immunofluorescence images and quantification of E-cadherin and N-cadherin in the lungs ( n = 3, scale bar 50 μm). (J) Schematic diagram of the adhesion assay of LLC cells by NETs. (K) Representative fluorescence images of the adhesion assay for DiI-labeled LLC cells trapped within PMA- or Rg1-treated neutrophils. (L) RNA-seq analysis of neutrophils. GSEA of genes related to the regulation of vascular permeability. (M) Schematic diagram of the endothelial permeability assay. (N) The effects of Rg1 or NETs on vascular permeability were tested via an endothelial permeability assay. (O-P) Representative immunofluorescence images and quantification of CD31 and FITC-dextran in the lungs ( n = 3; scale bar, 50 μm). All the data are presented as mean ± SEM. # p < 0.05, #### p < 0.0001 vs. the control group. * p < 0.05, ** p < 0.01, **** p < 0.0001 vs. the model group.
    Figure Legend Snippet: Rg1 reversed NET-induced invasion, EMT, and adhesion of LLC cells and increased vascular permeability. (A) LLC cell proliferation was detected via a CCK8 assay. (B) Schematic diagram of NET isolation and coculture with LLC cells in the transwell system. (C) Images of the transwell invasion assay (scale bar, 200 μm). The number of infiltrated cells and the OD values were used for quantification. (D-E) Representative immunofluorescence images and quantification of MMP9 in the lungs (n = 3, scale bar 50 μm). (F) Representative immunofluorescence images of E-cadherin and N-cadherin in LLC cells treated with or without Rg1 or NETs. (G-I) Representative immunofluorescence images and quantification of E-cadherin and N-cadherin in the lungs ( n = 3, scale bar 50 μm). (J) Schematic diagram of the adhesion assay of LLC cells by NETs. (K) Representative fluorescence images of the adhesion assay for DiI-labeled LLC cells trapped within PMA- or Rg1-treated neutrophils. (L) RNA-seq analysis of neutrophils. GSEA of genes related to the regulation of vascular permeability. (M) Schematic diagram of the endothelial permeability assay. (N) The effects of Rg1 or NETs on vascular permeability were tested via an endothelial permeability assay. (O-P) Representative immunofluorescence images and quantification of CD31 and FITC-dextran in the lungs ( n = 3; scale bar, 50 μm). All the data are presented as mean ± SEM. # p < 0.05, #### p < 0.0001 vs. the control group. * p < 0.05, ** p < 0.01, **** p < 0.0001 vs. the model group.

    Techniques Used: Permeability, CCK-8 Assay, Isolation, Transwell Invasion Assay, Immunofluorescence, Cell Adhesion Assay, Fluorescence, Labeling, RNA Sequencing, Control

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    Isolation:

    Article Title: SREBP2 regulates CCDC25 expression and promotes tumor metastasis in Triple-Negative Breast Cancer.
    Article Snippet: .. To obtain neutrophils for this study, human peripheral blood samples from a healthy donor were processed following the instructions of the Human Peripheral Blood Neutrophil Isolation Solution Kit (Solarbio, Beijing, China). ..

    Article Title: Nanocatalytic anti-inflammatory therapy for alleviating rheumatoid lesions with endothelial cell-derived hydrosilicene
    Article Snippet: After co-culture with HSI nanosheets and HSI@ECM at concentrations of 0, 50, 100 and 200 μg/mL for durations of 24, 48, and 72 h, 100 μL of CCK-8 working solution (Beyotime, China) was added to each well and incubated in darkness at 37 °C for 2 h. Subsequently, the absorbance at 450 nm was measured using a microplate reader (Infinite E Plex, Tecan, Switzerland). .. 2.7 Neutrophil isolation and cell culture Human peripheral blood neutrophils were isolated from a healthy donor using a density gradient centrifugation kit (Solarbio, China). ..

    Article Title: Single-cell RNA sequencing highlights the heterogeneity of neutrophils in granulomatosis with polyangiitis
    Article Snippet: .. Neutrophils were isolated utilizing the Human Peripheral Blood Neutrophil Isolation Kit (Solarbio, China), then stimulated with eBioscience Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) (Thermo Fisher Scitific, USA) at 37°C in 5% CO 2 . .. For intracellular staining, cells were fixed and permeabilized (Cytofix/Cytoperm and Perm/Wash Buffer, BD Biosciences, USA), then incubated with PLA2G7 polyclonal antibody at 37°C in 5% CO 2 for 1.5 h, followed by FITC-conjugated goat anti-rabbit IgG (Proteintech, China) for 1 h. Stained neutrophils were analyzed on a BD FACSAria II flow cytometer, and data processed using FlowJo v10.8.

    Article Title: Robust transcriptomic hallmarks targeting intratumor heterogeneity in intrahepatic cholangiocarcinoma
    Article Snippet: Cell Counting Kit (CCK-8) , Yeasen , Cat# 40203ES76. .. Human Peripheral Blood Neutrophil Isolation Solution Kit , Solarbio , Cat# P9040. ..

    Article Title: Ginkgolide B Alleviates Airway Inflammation in Hyperoxia Lung Injury
    Article Snippet: .. Human neutrophils were isolated from fresh peripheral blood of healthy neonates according to the instructions of the peripheral blood neutrophil isolation kit (Cat # P9040, Beijing Solarbio Science & Technology Co. Ltd). .. The isolated neutrophils were counted and placed in a 24‐well plate, stimulated with PAF (Cat # HY‐108635, MCE) at 100 ng/mL for 0.5, 2, and 4 h. Then, some neutrophils were collected at each time point for flow cytometry detection.

    Article Title: Ginkgolide B Alleviates Airway Inflammation in Hyperoxia Lung Injury.
    Article Snippet: .. Human neutrophils were isolated from fresh peripheral blood of healthy neonates according to the instructions of the peripheral blood neutrophil isolation kit (Cat#P9040, Beijing Solarbio Science & Technology Co. Ltd). .. The isolated neutrophils were counted and placed in a 24‐well plate, stimulated with PAF (Cat# HY‐108635, MCE) at 100 ng/mL for 0.5, 2, and 4 h. Then, some neutrophils were collected at each time point for flow cytometry detection.

    Cell Culture:

    Article Title: Nanocatalytic anti-inflammatory therapy for alleviating rheumatoid lesions with endothelial cell-derived hydrosilicene
    Article Snippet: After co-culture with HSI nanosheets and HSI@ECM at concentrations of 0, 50, 100 and 200 μg/mL for durations of 24, 48, and 72 h, 100 μL of CCK-8 working solution (Beyotime, China) was added to each well and incubated in darkness at 37 °C for 2 h. Subsequently, the absorbance at 450 nm was measured using a microplate reader (Infinite E Plex, Tecan, Switzerland). .. 2.7 Neutrophil isolation and cell culture Human peripheral blood neutrophils were isolated from a healthy donor using a density gradient centrifugation kit (Solarbio, China). ..

    Gradient Centrifugation:

    Article Title: Nanocatalytic anti-inflammatory therapy for alleviating rheumatoid lesions with endothelial cell-derived hydrosilicene
    Article Snippet: After co-culture with HSI nanosheets and HSI@ECM at concentrations of 0, 50, 100 and 200 μg/mL for durations of 24, 48, and 72 h, 100 μL of CCK-8 working solution (Beyotime, China) was added to each well and incubated in darkness at 37 °C for 2 h. Subsequently, the absorbance at 450 nm was measured using a microplate reader (Infinite E Plex, Tecan, Switzerland). .. 2.7 Neutrophil isolation and cell culture Human peripheral blood neutrophils were isolated from a healthy donor using a density gradient centrifugation kit (Solarbio, China). ..

    other:

    Article Title: Robust transcriptomic hallmarks targeting intratumor heterogeneity in intrahepatic cholangiocarcinoma.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Recombinant human EGF Peprotech Cat# PHG0311 Insulin-Transferrin-SeleniumSodium Pyruvate Gibco Cat# 51300044 Ganetespib Selleck Cat# S1159 Alvespimycin Selleck Cat# S1142 TAE684 Selleck Cat# S1108 ABT-263 Selleck Cat# S1001 dBET6 Selleck Cat# S8762 EDTA buffer Servicebio Cat# G1203 Citrate buffer Servicebio Cat# G1202 Endogenous peroxidase blocking solution YIFAN Biological Cat# YFH4001 Goat serum BOSTER Cat# AR0009 Diaminobenzidine solution Servicebio Cat# G1212 Lysis buffer Biosharp Cat# BL504A Proteinase inhibitor cocktail Yeasen Cat# 20124ES Phosphorylated protease inhibitor cocktail Yeasen Cat# 20109ES Collagenase IV BioFroxx Cat# 2091GR001 Matrigel Corning Cat# 356231 Advanced DMEM/F12 Gibco Cat# 12634010 Penicillin-Streptomycin Gibco Cat# 15070063 Primocin InvivoGen Cat# ant-pm-2 Glutamax Gibco Cat# 35050061 HEPES Gibco Cat# 15630080 B-27 Supplement Gibco Cat# 17504044 N-2 Supplement Gibco Cat# 17502048 N-acetylcysteine Sigma Cat# A9165 R-spondin 1 Sino Biological Cat# 11083-HNAS Noggin Sino Biological Cat# 50688-M02H Nicotinamide Sigma Cat# N0636 Gastrin Sigma Cat# G9145 EGF Peprotech Cat# AF-100-15-500 FGF10 Peprotech Cat# 100-26-25 Forskolin MedChemExpress Cat# HY-15371 Y27632 Selleck Cat# S1049 A83-01 MedChemExpress Cat# HY-10432 TrypLE Express Gibco Cat# 12604021 Critical commercial assays Cell Counting Kit (CCK-8) Yeasen Cat# 40203ES76 Human Peripheral Blood Neutrophil Isolation Solution Kit Solarbio Cat# P9040 MolPure TRIeasy Plus Total RNA Kit Yeasen Cat# 19211ES Hifair III 1st Strand cDNA Synthesis SuperMix Yeasen Cat# 11141ES Hieff qPCR SYBR Green Master Mix Yeasen Cat# 11202ES All-in-One Tricine-PAGE Kit Epizyme Biomedical Technology Cat# PG310 (Continued on next page) Cell Reports Medicine 7, 102708, April 21, 2026 e2 Article ll OPEN ACCESS

    Article Title: Robust transcriptomic hallmarks targeting intratumor heterogeneity in intrahepatic cholangiocarcinoma
    Article Snippet: Proteins were separated by Tricine-PAGE (Epizyme Biomedical Technology, PG310) and transferred to polyvinylidene fluoride (PVDF) membranes (Epizyme Biomedical Technology, WJ001).

    Cell Stimulation:

    Article Title: Single-cell RNA sequencing highlights the heterogeneity of neutrophils in granulomatosis with polyangiitis
    Article Snippet: .. Neutrophils were isolated utilizing the Human Peripheral Blood Neutrophil Isolation Kit (Solarbio, China), then stimulated with eBioscience Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) (Thermo Fisher Scitific, USA) at 37°C in 5% CO 2 . .. For intracellular staining, cells were fixed and permeabilized (Cytofix/Cytoperm and Perm/Wash Buffer, BD Biosciences, USA), then incubated with PLA2G7 polyclonal antibody at 37°C in 5% CO 2 for 1.5 h, followed by FITC-conjugated goat anti-rabbit IgG (Proteintech, China) for 1 h. Stained neutrophils were analyzed on a BD FACSAria II flow cytometer, and data processed using FlowJo v10.8.



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    Figure 7. CGP60474 and MAPK signaling. (a) Representative phosphokinome array for KF-1762 <t>(Proteome</t> Profiler—Human Phospho- Kinase Array—(R&D Systems, <t>ARY003C)</t> showing most upregulated phosphoproteins (p-ErkT202/Y204,T185/
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    https://www.bioz.com/product/human+phosphorylated+rtk+array+kit/Proteome+Profiler+Human+Phospho-Kinase+Array+Kit/pm38303762-175-4-25
    Average 96 stars, based on 1 article reviews
    phosphorylated rtk array proteome profiler human phospho kinase array kit - by Bioz Stars, 2026-11
    96/100 stars
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    Journal: iScience

    Article Title: Decoding sunitinib resistance in ccRCC: Metabolic-reprogramming-induced ABAT and GABAergic system shifts

    doi: 10.1016/j.isci.2024.110415

    Figure Lengend Snippet:

    Article Snippet: Human RTK Phosphorylation Antibody Array Kit , RayBiotech , Cat# AAH-PRTK-1-8.

    Techniques: Recombinant, Lysis, Electrophoresis, Cell Counting, Fluorescence, Staining, Phospho-proteomics, Ab Array, Plasmid Preparation, Software

    CSC effects on the expression of RTK activation upregulating downstream effector protein, MAPK, in human cervical epithelial cells. ( A ) Expression of growth factor RTKs in HPV-16 Ect/E6E7 cells shown by representative RTK array (top) and as quantitated dot blot intensity (0 and 10 µg/mL) values (mean + SE) at the bottom. ( B ) Western blotting for phosphorylated MAPK44/42. Data are represented as mean ± SE; * p < 0.05 compared with the control group. Significance was expressed as mean ± SE of at least three replicates.

    Journal: International Journal of Molecular Sciences

    Article Title: Tobacco Smoke Condensate Induces Morphologic Changes in Human Papillomavirus-Positive Cervical Epithelial Cells Consistent with Epithelial to Mesenchymal Transition (EMT) with Activation of Receptor Tyrosine Kinases and Regulation of TGFB

    doi: 10.3390/ijms25094902

    Figure Lengend Snippet: CSC effects on the expression of RTK activation upregulating downstream effector protein, MAPK, in human cervical epithelial cells. ( A ) Expression of growth factor RTKs in HPV-16 Ect/E6E7 cells shown by representative RTK array (top) and as quantitated dot blot intensity (0 and 10 µg/mL) values (mean + SE) at the bottom. ( B ) Western blotting for phosphorylated MAPK44/42. Data are represented as mean ± SE; * p < 0.05 compared with the control group. Significance was expressed as mean ± SE of at least three replicates.

    Article Snippet: The array was conducted following the manufacturer’s instructions to detect phosphorylated tyrosine sites on RTKs (#ARY001B, R&D Systems, Minneapolis, MN, USA).

    Techniques: Expressing, Activation Assay, Dot Blot, Western Blot, Control

    Figure 7. CGP60474 and MAPK signaling. (a) Representative phosphokinome array for KF-1762 (Proteome Profiler—Human Phospho- Kinase Array—(R&D Systems, ARY003C) showing most upregulated phosphoproteins (p-ErkT202/Y204,T185/

    Journal: JID innovations : skin science from molecules to population health

    Article Title: Identification of Collagen-Suppressive Agents in Keloidal Fibroblasts Using a High-Content, Phenotype-Based Drug Screen.

    doi: 10.1016/j.xjidi.2023.100248

    Figure Lengend Snippet: Figure 7. CGP60474 and MAPK signaling. (a) Representative phosphokinome array for KF-1762 (Proteome Profiler—Human Phospho- Kinase Array—(R&D Systems, ARY003C) showing most upregulated phosphoproteins (p-ErkT202/Y204,T185/

    Article Snippet: Phosphorylated kinase array and phosphorylated RTK array Proteome Profiler Human Phospho-Kinase Array Kit (ARY003C) and Proteome Profiler Human Phospho-RTK Array Kit (ARY001B) were purchased from R&D Systems (Minneapolis, MN).

    Techniques: