human pten elisa kit (Danaher Inc)

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Danaher Inc human pten elisa kit

The influence of incubation of cell lines with rec-TF and PAR2-activation on <t>PTEN</t> activity and Akt inhibition. Cells (2 × 10 5 ) were treated with ( A ) rec-TF (65 pg/ml) for 1 h, or ( B ) PAR2-AP for 30 min, lysed in PhosphoSafe buffer (150 µl) and the lipid-phosphatase activity of PTEN measured using the echelon PTEN Activity <t>ELISA</t> kit (n = 3; *p < 0.05 vs. the respective untreated samples). Akt activity was also determined in the samples treated with ( C ) rec-TF or ( D ) PAR2-AP as above, and measured using the Akt Kinase activity assay kit according to manufacturer's instructions (n = 3; *p < 0.05 vs. the respective untreated samples). Samples of MDA-MB-231, LoVo and CaCo-2 cells were pre-incubated with a PAR2 blocking antibody (SAM-11, 20 µg/ml) or a control isotype antibody. The cells were treated with recombinant TF and ( E ) PTEN activity and ( F ) Akt activity measured as above (n = 3; *p < 0.05 vs. the respective control isotype antibody).

Human Pten Elisa Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 871 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+phosphorylated+rtk+array+kit/Human+MER+ELISA+Kit/pmc07708427-61-30-34
Average 99 stars, based on 871 article reviews

human pten elisa kit - by Bioz Stars, 2026-11

99/100 stars

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1) Product Images from "Activation of PAR2 by tissue factor induces the release of the PTEN from MAGI proteins and regulates PTEN and Akt activities"

Article Title: Activation of PAR2 by tissue factor induces the release of the PTEN from MAGI proteins and regulates PTEN and Akt activities

Journal: Scientific Reports

doi: 10.1038/s41598-020-77963-6

The influence of incubation of cell lines with rec-TF and PAR2-activation on PTEN activity and Akt inhibition. Cells (2 × 10 5 ) were treated with ( A ) rec-TF (65 pg/ml) for 1 h, or ( B ) PAR2-AP for 30 min, lysed in PhosphoSafe buffer (150 µl) and the lipid-phosphatase activity of PTEN measured using the echelon PTEN Activity ELISA kit (n = 3; *p < 0.05 vs. the respective untreated samples). Akt activity was also determined in the samples treated with ( C ) rec-TF or ( D ) PAR2-AP as above, and measured using the Akt Kinase activity assay kit according to manufacturer's instructions (n = 3; *p < 0.05 vs. the respective untreated samples). Samples of MDA-MB-231, LoVo and CaCo-2 cells were pre-incubated with a PAR2 blocking antibody (SAM-11, 20 µg/ml) or a control isotype antibody. The cells were treated with recombinant TF and ( E ) PTEN activity and ( F ) Akt activity measured as above (n = 3; *p < 0.05 vs. the respective control isotype antibody).

Figure Legend Snippet: The influence of incubation of cell lines with rec-TF and PAR2-activation on PTEN activity and Akt inhibition. Cells (2 × 10 5 ) were treated with ( A ) rec-TF (65 pg/ml) for 1 h, or ( B ) PAR2-AP for 30 min, lysed in PhosphoSafe buffer (150 µl) and the lipid-phosphatase activity of PTEN measured using the echelon PTEN Activity ELISA kit (n = 3; *p < 0.05 vs. the respective untreated samples). Akt activity was also determined in the samples treated with ( C ) rec-TF or ( D ) PAR2-AP as above, and measured using the Akt Kinase activity assay kit according to manufacturer's instructions (n = 3; *p < 0.05 vs. the respective untreated samples). Samples of MDA-MB-231, LoVo and CaCo-2 cells were pre-incubated with a PAR2 blocking antibody (SAM-11, 20 µg/ml) or a control isotype antibody. The cells were treated with recombinant TF and ( E ) PTEN activity and ( F ) Akt activity measured as above (n = 3; *p < 0.05 vs. the respective control isotype antibody).

Techniques Used: Incubation, Activation Assay, Activity Assay, Inhibition, Enzyme-linked Immunosorbent Assay, Kinase Assay, Blocking Assay, Recombinant

The influence of long-term treatment of cell lines with rec-TF on cellular PTEN antigen levels and Akt activity. Equal number of cells were lysed in PhosphoSafe buffer (150 µl) and ( A ) the level of PTEN antigen measured using a PTEN ELISA kit (n = 5; *p < 0.05 vs. the samples on first day 0). ( B ) The Akt activity was also measured using the Akt Kinase activity assay kit (n = 3; *p < 0.05 vs. the samples on first day 0). Cells (2 × 10 5 ) were cultured in the recommended media and supplemented every 2 days with rec-TF (65 pg/ml) up to 14 days. ( C ) The number of cells in the treated and untreated samples were determined using crystal violet staining (n = 3; *p < 0.05 vs. the samples on first day 0). In addition, cells were lysed in PhosphoSafe buffer and ( D ) the level of PTEN antigen and ( E ) the Akt kinase activity measured. (n = 3; *p < 0.05 vs. the samples on first day 0).

Figure Legend Snippet: The influence of long-term treatment of cell lines with rec-TF on cellular PTEN antigen levels and Akt activity. Equal number of cells were lysed in PhosphoSafe buffer (150 µl) and ( A ) the level of PTEN antigen measured using a PTEN ELISA kit (n = 5; *p < 0.05 vs. the samples on first day 0). ( B ) The Akt activity was also measured using the Akt Kinase activity assay kit (n = 3; *p < 0.05 vs. the samples on first day 0). Cells (2 × 10 5 ) were cultured in the recommended media and supplemented every 2 days with rec-TF (65 pg/ml) up to 14 days. ( C ) The number of cells in the treated and untreated samples were determined using crystal violet staining (n = 3; *p < 0.05 vs. the samples on first day 0). In addition, cells were lysed in PhosphoSafe buffer and ( D ) the level of PTEN antigen and ( E ) the Akt kinase activity measured. (n = 3; *p < 0.05 vs. the samples on first day 0).

Techniques Used: Activity Assay, Enzyme-linked Immunosorbent Assay, Kinase Assay, Cell Culture, Staining

Clinical Proteomics:

Article Title: CREB1-driven CXCR4 hi neutrophils promote skin inflammation in mouse models and human patients
Article Snippet: After washing twice with PBS and subsequently analyzed by a confocal microscope (LSM880, Carl Zeiss). .. CXCL12 (E-EL-H0052c, ElabScience, Wuhan, China), IL17A (E-EL-H5812c, ElabScience), MPO (ab119605, Abcam), and MMP-9 (E-EL-H6075, ElabScience) levels in blood plasma samples were measured using ELISA kits according to the manufacturer’s instructions. ..

Enzyme-linked Immunosorbent Assay:

Article Title: Oral supplementation with fish cartilage hydrolysate in an adult population suffering from knee pain and function discomfort: results from an innovative approach combining an exploratory clinical study and an ex vivo clinical investigation
Article Snippet: Nitrate/Nitrite colorimetric assay and prostaglandin E2 Enzyme Immunoassay (EIA) kits were obtained from Cayman Chemical (Ann Arbor-MI, USA). .. Human ELISA Kits for MMP-13, Collagen II and Aggrecan detection were purchased from Abcam® (Paris, France). ..

Article Title: Single-Cell RNA-Sequencing Reveals Peripheral T Helper Cells Promoting the Development of IgG4-Related Disease by Enhancing B Cell Activation and Differentiation
Article Snippet: .. IL-21 level was detected using human IL-21 ELISA kit (ab119542, Abcam) according to the instructions. .. RNA was extracted from CD4 + T cell using Micro Cells Total RNA Extraction Kit (Simgen, Hangzhou, China) and quantified using spectro-photometry (Denovix DS-11).

Article Title: Serum autophagy protein 5 is positively related to T helper 2/T helper 1 ratio, inflammation, and exacerbation in adult asthma patients
Article Snippet: The levels of Th1 and Th2 cells in 125 asthma patients were measured with the Human Th1/Th2 Phenotyping Kit (BD Biosciences, USA; Cat. No. 560,751) by flow cytometry (FCM). .. Then, the levels of interleukin-4 (IL-4), interferon-gamma (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and interleukin-17 (IL-17), were detected among 200 asthma patients with Human ELISA Kits (Abcam, UK). ..

Article Title: Production of therapeutic levels of human FIX-R338L by engineered B cells using GMP-compatible medium
Article Snippet: .. FIX secretion levels in cell culture supernatants or in mouse plasmas were determined by ELISA according to manufacturer’s instructions (Human Factor IX ELISA Kit, Abcam, Paris, France). .. Human IgA, IgM, and IgG were measured using an MSD Multiplex assay according to manufacturer’s instructions (Meso Scale Diagnostics, Rockville, MD).

Article Title: Rationale and protocol of a double-blind, randomized, placebo-controlled trial to test the efficacy, safety, and tolerability of dimethyl fumarate in Friedreich Ataxia (DMF-FA-201)
Article Snippet: PBMCs will be lysed with an extraction buffer (Abcam n.AB193970-50, Cambridge, UK), and total protein will be measured using the bicinchoninic acid assay. .. Each sample will be assayed in duplicate using a commercially available ELISA kit (Frataxin profiling ELISA kit, n.AB110173, Abcam, Cambridge, UK), and will be normalized with a standard curve using full length human frataxin (n.AB110353, Abcam, Cambridge, UK). ..

Immunostaining:

Article Title: Hallmark molecular and pathological features of POLG disease are recapitulated in cerebral organoids
Article Snippet: Mounted sections were incubated for 1 hour at room temperature with and blocked using blocking buffer containing (Sigma-Aldrich, #G9023) and 0.1% (v/v) Triton X100 (Sigma-Aldrich, #9036-19-5), and then incubated with primary antibodies diluted in blocking solution overnight at 4°C. .. The following primary antibodies were used for immunostaining: Nanog (rabbit, 1:100; Abcam, #ab80892), Oct4 (rabbit, 1:100; Abcam, #ab19857), SSEA4 (mouse, 1:200; Abcam, #ab16287,), NeuN (rabbit, 1:500; Cell Signaling, #24307S), MAP2 (rabbit, 1:1000; Abcam, #ab5392), β-tubulin III (TUJ1) (mouse, 1:1000; Abcam, #ab78078), Synaptophysin (mouse, 1:500; Proteintech, #17785-1-AP), PSD-95 (rabbit, 1:500; Proteintech, #20665-1-AP), SOX2 (rabbit, 1:100; Abcam, #ab97959), NDUFB10 (rabbit, 1:350; Abcam 196019), TFAM (mouse, 1:1000; Abcam #ab119684), TOMM20 (mouse, 1:350; Abcam, #ab56783), GFAP (chicken, 1:500; Abcam #ab4674), DCX (mouse, 1:200; Abcam #ab135349), SATB2 (rabbit, 1:400; Abcam #ab4674), CTIP2 (rat, 1:500; Abcam, #ab18465), OLIG2 (rabbit, 1:500; Abcam, #ab42453). .. Alexa Fluor Dyes (Invitrogen, #A11008, #A21449, #A11012, #A21141, #A11042, #A21236) were used at 1:800 dilution as secondary antibodies.

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human pten elisa kit (Danaher Inc)

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1) Product Images from "Activation of PAR2 by tissue factor induces the release of the PTEN from MAGI proteins and regulates PTEN and Akt activities"

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