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cd34 cells  (Miltenyi Biotec)


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    Miltenyi Biotec cd34 cells
    Fig. 4 | Long-term selective expansion of functional human HSCs in 3a medium. a, The log2-transformed fold change in the expression of the indicated HSC-associated genes. Mean ± s.d. of three independent cultures. EPCR+ and EPCR− indicate CD34highEPCR+ cells and CD34highEPCR− cells, respectively. **†P = 0.0049; **‡P = 0.0043; **§P = 0.0022; ***P = 0.0006. b,c, Results from GSEA for genes differentially expressed between CD34highEPCR+ and CD34highEPCR− samples using gene sets for HSC genes (b) and mitochondrial oxidative-phosphorylation-related genes (c). Statistical significance was calculated using an empirical phenotype-based permutation test procedure38. NES, normalized enrichment score. d, Uniform manifold approximation and projection (UMAP) plot of single-cell RNA-seq data from 10-day-expanded <t>CD34+</t> cord blood cells with 12 cell clusters annotated (see ‘Single-cell RNA-seq’ in Methods for details of the abbreviations used). Integrated cell map from cells cultured in PCL-PVAc-PEG-based 3a medium, StemSpan with SR-1 medium
    Cd34 Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1651 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+phosphorylated+rtk+array+kit/CD34+MicroBead+Kit%2C+human/pm36813966-435-2-12
    Average 97 stars, based on 1651 article reviews
    cd34 cells - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Chemically defined cytokine-free expansion of human haematopoietic stem cells."

    Article Title: Chemically defined cytokine-free expansion of human haematopoietic stem cells.

    Journal: Nature

    doi: 10.1038/s41586-023-05739-9

    Fig. 4 | Long-term selective expansion of functional human HSCs in 3a medium. a, The log2-transformed fold change in the expression of the indicated HSC-associated genes. Mean ± s.d. of three independent cultures. EPCR+ and EPCR− indicate CD34highEPCR+ cells and CD34highEPCR− cells, respectively. **†P = 0.0049; **‡P = 0.0043; **§P = 0.0022; ***P = 0.0006. b,c, Results from GSEA for genes differentially expressed between CD34highEPCR+ and CD34highEPCR− samples using gene sets for HSC genes (b) and mitochondrial oxidative-phosphorylation-related genes (c). Statistical significance was calculated using an empirical phenotype-based permutation test procedure38. NES, normalized enrichment score. d, Uniform manifold approximation and projection (UMAP) plot of single-cell RNA-seq data from 10-day-expanded CD34+ cord blood cells with 12 cell clusters annotated (see ‘Single-cell RNA-seq’ in Methods for details of the abbreviations used). Integrated cell map from cells cultured in PCL-PVAc-PEG-based 3a medium, StemSpan with SR-1 medium
    Figure Legend Snippet: Fig. 4 | Long-term selective expansion of functional human HSCs in 3a medium. a, The log2-transformed fold change in the expression of the indicated HSC-associated genes. Mean ± s.d. of three independent cultures. EPCR+ and EPCR− indicate CD34highEPCR+ cells and CD34highEPCR− cells, respectively. **†P = 0.0049; **‡P = 0.0043; **§P = 0.0022; ***P = 0.0006. b,c, Results from GSEA for genes differentially expressed between CD34highEPCR+ and CD34highEPCR− samples using gene sets for HSC genes (b) and mitochondrial oxidative-phosphorylation-related genes (c). Statistical significance was calculated using an empirical phenotype-based permutation test procedure38. NES, normalized enrichment score. d, Uniform manifold approximation and projection (UMAP) plot of single-cell RNA-seq data from 10-day-expanded CD34+ cord blood cells with 12 cell clusters annotated (see ‘Single-cell RNA-seq’ in Methods for details of the abbreviations used). Integrated cell map from cells cultured in PCL-PVAc-PEG-based 3a medium, StemSpan with SR-1 medium

    Techniques Used: Functional Assay, Transformation Assay, Expressing, Phospho-proteomics, RNA Sequencing, Cell Culture

    Related Articles

    Isolation:

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch
    Article Snippet: .. CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ]. .. pTLCV2-sgRNA-Zeb1-up-exon4 (targeting Zeb1 intron 3, and containing a Cas9 expression cassette) and pLentiGuide-Hygro-sgZeb1-downEx6 (targeting Zeb1 intron 6) viral plasmids were generated by inserting annealed sgRNA primers (Table ) into pTLCV2 (Addgene #87360) and pLentiGuide-Hygro (Addgene #139462), respectively.

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Selection:

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch
    Article Snippet: .. CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ]. .. pTLCV2-sgRNA-Zeb1-up-exon4 (targeting Zeb1 intron 3, and containing a Cas9 expression cassette) and pLentiGuide-Hygro-sgZeb1-downEx6 (targeting Zeb1 intron 6) viral plasmids were generated by inserting annealed sgRNA primers (Table ) into pTLCV2 (Addgene #87360) and pLentiGuide-Hygro (Addgene #139462), respectively.

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Magnetic Cell Separation:

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch
    Article Snippet: .. CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ]. .. pTLCV2-sgRNA-Zeb1-up-exon4 (targeting Zeb1 intron 3, and containing a Cas9 expression cassette) and pLentiGuide-Hygro-sgZeb1-downEx6 (targeting Zeb1 intron 6) viral plasmids were generated by inserting annealed sgRNA primers (Table ) into pTLCV2 (Addgene #87360) and pLentiGuide-Hygro (Addgene #139462), respectively.

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Cell Culture:

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch
    Article Snippet: .. CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ]. .. pTLCV2-sgRNA-Zeb1-up-exon4 (targeting Zeb1 intron 3, and containing a Cas9 expression cassette) and pLentiGuide-Hygro-sgZeb1-downEx6 (targeting Zeb1 intron 6) viral plasmids were generated by inserting annealed sgRNA primers (Table ) into pTLCV2 (Addgene #87360) and pLentiGuide-Hygro (Addgene #139462), respectively.

    Ex Vivo:

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch
    Article Snippet: .. CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ]. .. pTLCV2-sgRNA-Zeb1-up-exon4 (targeting Zeb1 intron 3, and containing a Cas9 expression cassette) and pLentiGuide-Hygro-sgZeb1-downEx6 (targeting Zeb1 intron 6) viral plasmids were generated by inserting annealed sgRNA primers (Table ) into pTLCV2 (Addgene #87360) and pLentiGuide-Hygro (Addgene #139462), respectively.

    Gradient Centrifugation:

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Purification:

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Immunostaining:

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.



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    Figure Lengend Snippet:

    Article Snippet: Human RTK Phosphorylation Antibody Array Kit , RayBiotech , Cat# AAH-PRTK-1-8.

    Techniques: Recombinant, Lysis, Electrophoresis, Cell Counting, Fluorescence, Staining, Phospho-proteomics, Ab Array, Plasmid Preparation, Software

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    Article Snippet: The array was conducted following the manufacturer’s instructions to detect phosphorylated tyrosine sites on RTKs (#ARY001B, R&D Systems, Minneapolis, MN, USA).

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