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cd34 cells  (Miltenyi Biotec)


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    Miltenyi Biotec cd34 cells
    Fig. 4 | Long-term selective expansion of functional human HSCs in 3a medium. a, The log2-transformed fold change in the expression of the indicated HSC-associated genes. Mean ± s.d. of three independent cultures. EPCR+ and EPCR− indicate CD34highEPCR+ cells and CD34highEPCR− cells, respectively. **†P = 0.0049; **‡P = 0.0043; **§P = 0.0022; ***P = 0.0006. b,c, Results from GSEA for genes differentially expressed between CD34highEPCR+ and CD34highEPCR− samples using gene sets for HSC genes (b) and mitochondrial oxidative-phosphorylation-related genes (c). Statistical significance was calculated using an empirical phenotype-based permutation test procedure38. NES, normalized enrichment score. d, Uniform manifold approximation and projection (UMAP) plot of single-cell RNA-seq data from 10-day-expanded <t>CD34+</t> cord blood cells with 12 cell clusters annotated (see ‘Single-cell RNA-seq’ in Methods for details of the abbreviations used). Integrated cell map from cells cultured in PCL-PVAc-PEG-based 3a medium, StemSpan with SR-1 medium
    Cd34 Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1642 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+phosphorylated+rtk+array+kit/CD34+MicroBead+Kit%2C+human/pm36813966-435-2-12
    Average 97 stars, based on 1642 article reviews
    cd34 cells - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Chemically defined cytokine-free expansion of human haematopoietic stem cells."

    Article Title: Chemically defined cytokine-free expansion of human haematopoietic stem cells.

    Journal: Nature

    doi: 10.1038/s41586-023-05739-9

    Fig. 4 | Long-term selective expansion of functional human HSCs in 3a medium. a, The log2-transformed fold change in the expression of the indicated HSC-associated genes. Mean ± s.d. of three independent cultures. EPCR+ and EPCR− indicate CD34highEPCR+ cells and CD34highEPCR− cells, respectively. **†P = 0.0049; **‡P = 0.0043; **§P = 0.0022; ***P = 0.0006. b,c, Results from GSEA for genes differentially expressed between CD34highEPCR+ and CD34highEPCR− samples using gene sets for HSC genes (b) and mitochondrial oxidative-phosphorylation-related genes (c). Statistical significance was calculated using an empirical phenotype-based permutation test procedure38. NES, normalized enrichment score. d, Uniform manifold approximation and projection (UMAP) plot of single-cell RNA-seq data from 10-day-expanded CD34+ cord blood cells with 12 cell clusters annotated (see ‘Single-cell RNA-seq’ in Methods for details of the abbreviations used). Integrated cell map from cells cultured in PCL-PVAc-PEG-based 3a medium, StemSpan with SR-1 medium
    Figure Legend Snippet: Fig. 4 | Long-term selective expansion of functional human HSCs in 3a medium. a, The log2-transformed fold change in the expression of the indicated HSC-associated genes. Mean ± s.d. of three independent cultures. EPCR+ and EPCR− indicate CD34highEPCR+ cells and CD34highEPCR− cells, respectively. **†P = 0.0049; **‡P = 0.0043; **§P = 0.0022; ***P = 0.0006. b,c, Results from GSEA for genes differentially expressed between CD34highEPCR+ and CD34highEPCR− samples using gene sets for HSC genes (b) and mitochondrial oxidative-phosphorylation-related genes (c). Statistical significance was calculated using an empirical phenotype-based permutation test procedure38. NES, normalized enrichment score. d, Uniform manifold approximation and projection (UMAP) plot of single-cell RNA-seq data from 10-day-expanded CD34+ cord blood cells with 12 cell clusters annotated (see ‘Single-cell RNA-seq’ in Methods for details of the abbreviations used). Integrated cell map from cells cultured in PCL-PVAc-PEG-based 3a medium, StemSpan with SR-1 medium

    Techniques Used: Functional Assay, Transformation Assay, Expressing, Phospho-proteomics, RNA Sequencing, Cell Culture

    Related Articles

    Isolation:

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch
    Article Snippet: .. CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ]. .. pTLCV2-sgRNA-Zeb1-up-exon4 (targeting Zeb1 intron 3, and containing a Cas9 expression cassette) and pLentiGuide-Hygro-sgZeb1-downEx6 (targeting Zeb1 intron 6) viral plasmids were generated by inserting annealed sgRNA primers (Table ) into pTLCV2 (Addgene #87360) and pLentiGuide-Hygro (Addgene #139462), respectively.

    Selection:

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch
    Article Snippet: .. CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ]. .. pTLCV2-sgRNA-Zeb1-up-exon4 (targeting Zeb1 intron 3, and containing a Cas9 expression cassette) and pLentiGuide-Hygro-sgZeb1-downEx6 (targeting Zeb1 intron 6) viral plasmids were generated by inserting annealed sgRNA primers (Table ) into pTLCV2 (Addgene #87360) and pLentiGuide-Hygro (Addgene #139462), respectively.

    Magnetic Cell Separation:

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch
    Article Snippet: .. CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ]. .. pTLCV2-sgRNA-Zeb1-up-exon4 (targeting Zeb1 intron 3, and containing a Cas9 expression cassette) and pLentiGuide-Hygro-sgZeb1-downEx6 (targeting Zeb1 intron 6) viral plasmids were generated by inserting annealed sgRNA primers (Table ) into pTLCV2 (Addgene #87360) and pLentiGuide-Hygro (Addgene #139462), respectively.

    Cell Culture:

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch
    Article Snippet: .. CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ]. .. pTLCV2-sgRNA-Zeb1-up-exon4 (targeting Zeb1 intron 3, and containing a Cas9 expression cassette) and pLentiGuide-Hygro-sgZeb1-downEx6 (targeting Zeb1 intron 6) viral plasmids were generated by inserting annealed sgRNA primers (Table ) into pTLCV2 (Addgene #87360) and pLentiGuide-Hygro (Addgene #139462), respectively.

    Ex Vivo:

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch
    Article Snippet: .. CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ]. .. pTLCV2-sgRNA-Zeb1-up-exon4 (targeting Zeb1 intron 3, and containing a Cas9 expression cassette) and pLentiGuide-Hygro-sgZeb1-downEx6 (targeting Zeb1 intron 6) viral plasmids were generated by inserting annealed sgRNA primers (Table ) into pTLCV2 (Addgene #87360) and pLentiGuide-Hygro (Addgene #139462), respectively.



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    Image Search Results


    CSC effects on the expression of RTK activation upregulating downstream effector protein, MAPK, in human cervical epithelial cells. ( A ) Expression of growth factor RTKs in HPV-16 Ect/E6E7 cells shown by representative RTK array (top) and as quantitated dot blot intensity (0 and 10 µg/mL) values (mean + SE) at the bottom. ( B ) Western blotting for phosphorylated MAPK44/42. Data are represented as mean ± SE; * p < 0.05 compared with the control group. Significance was expressed as mean ± SE of at least three replicates.

    Journal: International Journal of Molecular Sciences

    Article Title: Tobacco Smoke Condensate Induces Morphologic Changes in Human Papillomavirus-Positive Cervical Epithelial Cells Consistent with Epithelial to Mesenchymal Transition (EMT) with Activation of Receptor Tyrosine Kinases and Regulation of TGFB

    doi: 10.3390/ijms25094902

    Figure Lengend Snippet: CSC effects on the expression of RTK activation upregulating downstream effector protein, MAPK, in human cervical epithelial cells. ( A ) Expression of growth factor RTKs in HPV-16 Ect/E6E7 cells shown by representative RTK array (top) and as quantitated dot blot intensity (0 and 10 µg/mL) values (mean + SE) at the bottom. ( B ) Western blotting for phosphorylated MAPK44/42. Data are represented as mean ± SE; * p < 0.05 compared with the control group. Significance was expressed as mean ± SE of at least three replicates.

    Article Snippet: The array was conducted following the manufacturer’s instructions to detect phosphorylated tyrosine sites on RTKs (#ARY001B, R&D Systems, Minneapolis, MN, USA).

    Techniques: Expressing, Activation Assay, Dot Blot, Western Blot, Control

    Figure 7. CGP60474 and MAPK signaling. (a) Representative phosphokinome array for KF-1762 (Proteome Profiler—Human Phospho- Kinase Array—(R&D Systems, ARY003C) showing most upregulated phosphoproteins (p-ErkT202/Y204,T185/

    Journal: JID innovations : skin science from molecules to population health

    Article Title: Identification of Collagen-Suppressive Agents in Keloidal Fibroblasts Using a High-Content, Phenotype-Based Drug Screen.

    doi: 10.1016/j.xjidi.2023.100248

    Figure Lengend Snippet: Figure 7. CGP60474 and MAPK signaling. (a) Representative phosphokinome array for KF-1762 (Proteome Profiler—Human Phospho- Kinase Array—(R&D Systems, ARY003C) showing most upregulated phosphoproteins (p-ErkT202/Y204,T185/

    Article Snippet: Phosphorylated kinase array and phosphorylated RTK array Proteome Profiler Human Phospho-Kinase Array Kit (ARY003C) and Proteome Profiler Human Phospho-RTK Array Kit (ARY001B) were purchased from R&D Systems (Minneapolis, MN).

    Techniques:

    ACK1 is a major Ceritinib target in MDA-MB 453. ( A ) The levels of phosphorylated tyrosine kinases in a panel of 73 RTKs were analyzed by a human RTK Phosphorylation Antibody Array C1 Kit (RayBiotech) after MDA-MB 453 cells were treated with 10 μM ceritinib, DMSO was used as control. The positive dot#1,#2,#3 are all controlled amount of biotinylated antibody, which is consistent from array to array. As such, the intensity of these Positive Control signals serves much like housekeeping proteins. ( B ) The densitometric values of phospho-RTKs were determined by an image lab software (Bio-Rad). The relative intensities of the duplicated spots were normalized to positive control 1 spots. Values represent the mean of duplicate spots for each protein after normalization. ( C ) AR and ACK1 were determined by WB assay in human breast cancer cell lines and ( D ) in human breast cancer tissues

    Journal: Molecular Cancer

    Article Title: Ceritinib is a novel triple negative breast cancer therapeutic agent

    doi: 10.1186/s12943-022-01601-0

    Figure Lengend Snippet: ACK1 is a major Ceritinib target in MDA-MB 453. ( A ) The levels of phosphorylated tyrosine kinases in a panel of 73 RTKs were analyzed by a human RTK Phosphorylation Antibody Array C1 Kit (RayBiotech) after MDA-MB 453 cells were treated with 10 μM ceritinib, DMSO was used as control. The positive dot#1,#2,#3 are all controlled amount of biotinylated antibody, which is consistent from array to array. As such, the intensity of these Positive Control signals serves much like housekeeping proteins. ( B ) The densitometric values of phospho-RTKs were determined by an image lab software (Bio-Rad). The relative intensities of the duplicated spots were normalized to positive control 1 spots. Values represent the mean of duplicate spots for each protein after normalization. ( C ) AR and ACK1 were determined by WB assay in human breast cancer cell lines and ( D ) in human breast cancer tissues

    Article Snippet: To determine which receptor tyrosine kinases (RTKs) are targeted by ceritinib, the Human RTK Phosphorylation Antibody Array C1 Kit (RayBiotech, Cat. No. AAH-PRTK-1-2) was used.

    Techniques: Ab Array, Positive Control, Software