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vpc23019 857360p  (Croda International Plc)

 
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    Croda International Plc vpc23019 857360p
    Vpc23019 857360p, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 90/100, based on 201 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+phosphorylated+rtk+array+kit/VPC+23019/pm31915150-233-16-18
    Average 90 stars, based on 201 article reviews
    vpc23019 857360p - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Saline:

    Article Title: Apolipoprotein M bound sphingosine 1-phosphate suppresses NETosis through activating S1P1 and S1P4.
    Article Snippet: .. Phosphate-buffered saline (PBS) containing 0.4% fatty acid-free BSA (A8806, Sigma-Aldrich Co., St. Louis, MO) was used as the vehicle for VPC23019 (VPC, 857360 P; Avanti Polar Lipids) and CYM50358 (CYM, HY-136462; MedChemExpress Inc., Monmouth Junction, NJ) and 2% dimethyl sulfoxide (DMSO) in 0.9% saline was used as the vehicle for W146 (10009109; Cayman Chemical, Ann Arbor, MI). ..

    Cell Culture:

    Article Title: The autotaxin-LPA axis promotes membrane trafficking and secretion in yolk sac visceral endoderm cells.
    Article Snippet: Ex vivo whole-embryo culture Whole-embryo culture was performed as described previously (Koike et al., 2009). .. In brief, embryos were dissected at E7.5 and cultured in 100% rat serum (Charles River) supplemented with 2 mg/ml glucose in a culture bottle placed in a rotation drum culture system (Ikemoto Rika) at 37°C under 5% O2, 5% CO2, and 90% N2 for 24 h. In the pharmacologic experiments, embryos were incubated with the following reagents: 3-(4-[4-([1-(2- chlorophenyl)ethoxy]carbonyl amino)-3-methyl-5-isoxazolyl]benzylsulfanyl) propanoic acid (Ki16425, Sigma; 10 μM), (R)-phosphoric acid mono-[2amino-2-(3-octyl-phenylcarbamoyl)-ethyl] ester (VPC23019, Avanti Polar Lipid; 10 μM), and (S)-(+)-2-methyl-1-[(4-methyl-5-isoquinolynyl)sulfonyl] homopiperazine dihydrochloride (H1152, Calbiochem; 0.1 mM), phenylarsine oxide (Sigma-Aldrich; 0.1 μM), monensin (Nacalai Tesque; 50 nM), leupeptin (Peptide Institute; 25 μg/ml), 5-(N-ethyl-N- isopropyl)amiloride (EIPA, MedChemExpress; 50 μM), dynasore (Tocris Bioscience; 20 μM), and pitstop 2 (Selleck Chemicals, 10 μM). .. Total RNA was isolated from yolk sacs of E8.5 mice by use of Sepasol I (Nacalai Tesque).

    Incubation:

    Article Title: The autotaxin-LPA axis promotes membrane trafficking and secretion in yolk sac visceral endoderm cells.
    Article Snippet: Ex vivo whole-embryo culture Whole-embryo culture was performed as described previously (Koike et al., 2009). .. In brief, embryos were dissected at E7.5 and cultured in 100% rat serum (Charles River) supplemented with 2 mg/ml glucose in a culture bottle placed in a rotation drum culture system (Ikemoto Rika) at 37°C under 5% O2, 5% CO2, and 90% N2 for 24 h. In the pharmacologic experiments, embryos were incubated with the following reagents: 3-(4-[4-([1-(2- chlorophenyl)ethoxy]carbonyl amino)-3-methyl-5-isoxazolyl]benzylsulfanyl) propanoic acid (Ki16425, Sigma; 10 μM), (R)-phosphoric acid mono-[2amino-2-(3-octyl-phenylcarbamoyl)-ethyl] ester (VPC23019, Avanti Polar Lipid; 10 μM), and (S)-(+)-2-methyl-1-[(4-methyl-5-isoquinolynyl)sulfonyl] homopiperazine dihydrochloride (H1152, Calbiochem; 0.1 mM), phenylarsine oxide (Sigma-Aldrich; 0.1 μM), monensin (Nacalai Tesque; 50 nM), leupeptin (Peptide Institute; 25 μg/ml), 5-(N-ethyl-N- isopropyl)amiloride (EIPA, MedChemExpress; 50 μM), dynasore (Tocris Bioscience; 20 μM), and pitstop 2 (Selleck Chemicals, 10 μM). .. Total RNA was isolated from yolk sacs of E8.5 mice by use of Sepasol I (Nacalai Tesque).



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    Figure 7. CGP60474 and MAPK signaling. (a) Representative phosphokinome array for KF-1762 <t>(Proteome</t> Profiler—Human Phospho- Kinase Array—(R&D Systems, <t>ARY003C)</t> showing most upregulated phosphoproteins (p-ErkT202/Y204,T185/
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    Journal: iScience

    Article Title: Decoding sunitinib resistance in ccRCC: Metabolic-reprogramming-induced ABAT and GABAergic system shifts

    doi: 10.1016/j.isci.2024.110415

    Figure Lengend Snippet:

    Article Snippet: Human RTK Phosphorylation Antibody Array Kit , RayBiotech , Cat# AAH-PRTK-1-8.

    Techniques: Recombinant, Lysis, Electrophoresis, Cell Counting, Fluorescence, Staining, Phospho-proteomics, Ab Array, Plasmid Preparation, Software

    CSC effects on the expression of RTK activation upregulating downstream effector protein, MAPK, in human cervical epithelial cells. ( A ) Expression of growth factor RTKs in HPV-16 Ect/E6E7 cells shown by representative RTK array (top) and as quantitated dot blot intensity (0 and 10 µg/mL) values (mean + SE) at the bottom. ( B ) Western blotting for phosphorylated MAPK44/42. Data are represented as mean ± SE; * p < 0.05 compared with the control group. Significance was expressed as mean ± SE of at least three replicates.

    Journal: International Journal of Molecular Sciences

    Article Title: Tobacco Smoke Condensate Induces Morphologic Changes in Human Papillomavirus-Positive Cervical Epithelial Cells Consistent with Epithelial to Mesenchymal Transition (EMT) with Activation of Receptor Tyrosine Kinases and Regulation of TGFB

    doi: 10.3390/ijms25094902

    Figure Lengend Snippet: CSC effects on the expression of RTK activation upregulating downstream effector protein, MAPK, in human cervical epithelial cells. ( A ) Expression of growth factor RTKs in HPV-16 Ect/E6E7 cells shown by representative RTK array (top) and as quantitated dot blot intensity (0 and 10 µg/mL) values (mean + SE) at the bottom. ( B ) Western blotting for phosphorylated MAPK44/42. Data are represented as mean ± SE; * p < 0.05 compared with the control group. Significance was expressed as mean ± SE of at least three replicates.

    Article Snippet: The array was conducted following the manufacturer’s instructions to detect phosphorylated tyrosine sites on RTKs (#ARY001B, R&D Systems, Minneapolis, MN, USA).

    Techniques: Expressing, Activation Assay, Dot Blot, Western Blot, Control

    Figure 7. CGP60474 and MAPK signaling. (a) Representative phosphokinome array for KF-1762 (Proteome Profiler—Human Phospho- Kinase Array—(R&D Systems, ARY003C) showing most upregulated phosphoproteins (p-ErkT202/Y204,T185/

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    Figure Lengend Snippet: Figure 7. CGP60474 and MAPK signaling. (a) Representative phosphokinome array for KF-1762 (Proteome Profiler—Human Phospho- Kinase Array—(R&D Systems, ARY003C) showing most upregulated phosphoproteins (p-ErkT202/Y204,T185/

    Article Snippet: Phosphorylated kinase array and phosphorylated RTK array Proteome Profiler Human Phospho-Kinase Array Kit (ARY003C) and Proteome Profiler Human Phospho-RTK Array Kit (ARY001B) were purchased from R&D Systems (Minneapolis, MN).

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