Review



human lambda light chain elisa kit  (Bethyl)


Bioz Verified Symbol Bethyl is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Bethyl human lambda light chain elisa kit
    Human Lambda Light Chain Elisa Kit, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lambda+light+chain+kit/Human+Lambda+ELISA+Kit/pm40043324-383-15-21
    Average 93 stars, based on 52 article reviews
    human lambda light chain elisa kit - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Generated:

    Article Title: Impact of fixed phosphorus position on activity of triazole bisphosphonates as geranylgeranyl diphosphate synthase inhibitors.
    Article Snippet: Geranylgeranyl diphosphate synthase (GGDPS) produces the 20-carbon isoprenoid species used in protein geranylgeranylation reactions.. Inhibition of GGDPS has emerged as a novel means of disrupting the activity of geranylgeranylated proteins in cancers such as myeloma and osteosarcoma.. We have focused on developing a series of isoprenoid triazole bisphosphonate-based GGDPS inhibitors, demonstrating a complex structure–activity relationship (SAR), not only at the enzymatic level, but also at the cellular and whole organism levels.

    Article Title: Incorporation of an alpha-hydroxy substituent modulates activity of triazole bisphosphonate-based geranylgeranyl diphosphate synthase inhibitors.
    Article Snippet: Geranylgeranyl diphosphate synthase (GGDPS), the enzyme responsible for producing the isoprenoid substrate used in protein geranylgeranylation reactions, represents a novel therapeutic target in cancer, including malignancies such as multiple myeloma and osteosarcoma.. Our work has focused on the development of a series of isoprenoid triazole bisphosphonate-based GGDPS inhibitors.. Previous work has revealed that our lead GGDPS inhibitors do achieve substantial systemic distribution, in contrast to clinically used nitrogenous bisphosphonates which accumulate primarily in the bone.

    Western Blot:

    Article Title: Impact of fixed phosphorus position on activity of triazole bisphosphonates as geranylgeranyl diphosphate synthase inhibitors.
    Article Snippet: Geranylgeranyl diphosphate synthase (GGDPS) produces the 20-carbon isoprenoid species used in protein geranylgeranylation reactions.. Inhibition of GGDPS has emerged as a novel means of disrupting the activity of geranylgeranylated proteins in cancers such as myeloma and osteosarcoma.. We have focused on developing a series of isoprenoid triazole bisphosphonate-based GGDPS inhibitors, demonstrating a complex structure–activity relationship (SAR), not only at the enzymatic level, but also at the cellular and whole organism levels.

    Article Title: Incorporation of an alpha-hydroxy substituent modulates activity of triazole bisphosphonate-based geranylgeranyl diphosphate synthase inhibitors.
    Article Snippet: Geranylgeranyl diphosphate synthase (GGDPS), the enzyme responsible for producing the isoprenoid substrate used in protein geranylgeranylation reactions, represents a novel therapeutic target in cancer, including malignancies such as multiple myeloma and osteosarcoma.. Our work has focused on the development of a series of isoprenoid triazole bisphosphonate-based GGDPS inhibitors.. Previous work has revealed that our lead GGDPS inhibitors do achieve substantial systemic distribution, in contrast to clinically used nitrogenous bisphosphonates which accumulate primarily in the bone.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Impact of fixed phosphorus position on activity of triazole bisphosphonates as geranylgeranyl diphosphate synthase inhibitors.
    Article Snippet: Geranylgeranyl diphosphate synthase (GGDPS) produces the 20-carbon isoprenoid species used in protein geranylgeranylation reactions.. Inhibition of GGDPS has emerged as a novel means of disrupting the activity of geranylgeranylated proteins in cancers such as myeloma and osteosarcoma.. We have focused on developing a series of isoprenoid triazole bisphosphonate-based GGDPS inhibitors, demonstrating a complex structure–activity relationship (SAR), not only at the enzymatic level, but also at the cellular and whole organism levels.

    Article Title: Super enhancer acquisition drives expression of oncogenic PPP1R15B that regulates protein homeostasis in multiple myeloma.
    Article Snippet: .. The supernatants were collected for analysis of the secreted human immunoglobulin λ or κ light chains by enzyme-linked immunosorbent assay (ELISA), according to the manufacturer’s instructions (E88-115, E88-116; Bethyl Laboratories, Montgomery, TX, USA). ..

    Article Title: Incorporation of an alpha-hydroxy substituent modulates activity of triazole bisphosphonate-based geranylgeranyl diphosphate synthase inhibitors.
    Article Snippet: Geranylgeranyl diphosphate synthase (GGDPS), the enzyme responsible for producing the isoprenoid substrate used in protein geranylgeranylation reactions, represents a novel therapeutic target in cancer, including malignancies such as multiple myeloma and osteosarcoma.. Our work has focused on the development of a series of isoprenoid triazole bisphosphonate-based GGDPS inhibitors.. Previous work has revealed that our lead GGDPS inhibitors do achieve substantial systemic distribution, in contrast to clinically used nitrogenous bisphosphonates which accumulate primarily in the bone.

    Article Title: The oligosaccharyltransferase complex is an essential component of multiple myeloma plasma cells
    Article Snippet: MYC and 523 GADPH mRNAs were detected by real-time PCR (QuantStudio 3, Applied Biosystems, USA). .. 524 525 Human lambda light chain ELISA 526 The Nunc 96-well plates (Thermo Scientific) were coated with goat anti-human lambda chain 527 antibody (20 μg/mL) (Southern Biotechnology, #2070-01) in coating buffer (Human Lambda 528 ELISA Kit, Bethyl Laboratories, #E88116) and incubated overnight at 4°C. ..

    Article Title: The oligosaccharyltransferase complex is an essential component of multiple myeloma plasma cells
    Article Snippet: MYC and GADPH mRNAs were detected by real-time PCR (QuantStudio 3, Applied Biosystems, USA). .. The Nunc 96-well plates (Thermo Scientific) were coated with goat anti-human lambda chain antibody (20 μg/mL) (Southern Biotechnology, #2070-01) in coating buffer (Human Lambda ELISA Kit, Bethyl Laboratories, #E88116) and incubated overnight at 4°C. ..

    Article Title: EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction
    Article Snippet: .. Human λ chains ELISA Kits were purchased from Bethyl Laboratories (#E88-116). ..

    Biomarker Discovery:

    Article Title: Targeting NOTCH3 to eradicate dormant and therapy-resistant multiple myeloma cells
    Article Snippet: .. The levels of the tumor biomarker IgG2B (Invitrogen; Cat#88-50430-88), a paraprotein produced by 5TGM1 cells, or human lambda light chain (Bethyl Laboratories, Montgomery, TX, USA; Cat#E88-116), were used to determine tumor growth/burden in vivo (serum) or ex vivo (conditioned media). .. Cell lysates (50 μg) were boiled in the presence of SDS sample buffer (NuPAGE LDS sample buffer; Invitrogen) for 10 min and then subjected to electrophoresis on 10% SDS-PAGE (Bio-Rad Laboratories).

    Article Title: Targeting NOTCH3 to eradicate dormant and therapy-resistant multiple myeloma cells.
    Article Snippet: Gene expression was quantified by quantitative real-time PCR (qPCR) using TaqMan assays from Applied Biosystems (Foster City, CA, USA), according to the manufacturer's instructions. .. The levels of the tumor biomarker IgG2B (Invitrogen; Cat#88-50430-88), a paraprotein produced by 5TGM1 cells, or human lambda light chain (Bethyl Laboratories, Montgomery, TX, USA; Cat#E88-116), were used to determine tumor growth/burden in vivo (serum) or ex vivo (conditioned media). ..

    Produced:

    Article Title: Targeting NOTCH3 to eradicate dormant and therapy-resistant multiple myeloma cells
    Article Snippet: .. The levels of the tumor biomarker IgG2B (Invitrogen; Cat#88-50430-88), a paraprotein produced by 5TGM1 cells, or human lambda light chain (Bethyl Laboratories, Montgomery, TX, USA; Cat#E88-116), were used to determine tumor growth/burden in vivo (serum) or ex vivo (conditioned media). .. Cell lysates (50 μg) were boiled in the presence of SDS sample buffer (NuPAGE LDS sample buffer; Invitrogen) for 10 min and then subjected to electrophoresis on 10% SDS-PAGE (Bio-Rad Laboratories).

    Article Title: Targeting NOTCH3 to eradicate dormant and therapy-resistant multiple myeloma cells.
    Article Snippet: Gene expression was quantified by quantitative real-time PCR (qPCR) using TaqMan assays from Applied Biosystems (Foster City, CA, USA), according to the manufacturer's instructions. .. The levels of the tumor biomarker IgG2B (Invitrogen; Cat#88-50430-88), a paraprotein produced by 5TGM1 cells, or human lambda light chain (Bethyl Laboratories, Montgomery, TX, USA; Cat#E88-116), were used to determine tumor growth/burden in vivo (serum) or ex vivo (conditioned media). ..

    In Vivo:

    Article Title: Targeting NOTCH3 to eradicate dormant and therapy-resistant multiple myeloma cells
    Article Snippet: .. The levels of the tumor biomarker IgG2B (Invitrogen; Cat#88-50430-88), a paraprotein produced by 5TGM1 cells, or human lambda light chain (Bethyl Laboratories, Montgomery, TX, USA; Cat#E88-116), were used to determine tumor growth/burden in vivo (serum) or ex vivo (conditioned media). .. Cell lysates (50 μg) were boiled in the presence of SDS sample buffer (NuPAGE LDS sample buffer; Invitrogen) for 10 min and then subjected to electrophoresis on 10% SDS-PAGE (Bio-Rad Laboratories).

    Article Title: Targeting NOTCH3 to eradicate dormant and therapy-resistant multiple myeloma cells.
    Article Snippet: Gene expression was quantified by quantitative real-time PCR (qPCR) using TaqMan assays from Applied Biosystems (Foster City, CA, USA), according to the manufacturer's instructions. .. The levels of the tumor biomarker IgG2B (Invitrogen; Cat#88-50430-88), a paraprotein produced by 5TGM1 cells, or human lambda light chain (Bethyl Laboratories, Montgomery, TX, USA; Cat#E88-116), were used to determine tumor growth/burden in vivo (serum) or ex vivo (conditioned media). ..

    Ex Vivo:

    Article Title: Targeting NOTCH3 to eradicate dormant and therapy-resistant multiple myeloma cells
    Article Snippet: .. The levels of the tumor biomarker IgG2B (Invitrogen; Cat#88-50430-88), a paraprotein produced by 5TGM1 cells, or human lambda light chain (Bethyl Laboratories, Montgomery, TX, USA; Cat#E88-116), were used to determine tumor growth/burden in vivo (serum) or ex vivo (conditioned media). .. Cell lysates (50 μg) were boiled in the presence of SDS sample buffer (NuPAGE LDS sample buffer; Invitrogen) for 10 min and then subjected to electrophoresis on 10% SDS-PAGE (Bio-Rad Laboratories).

    Article Title: Targeting NOTCH3 to eradicate dormant and therapy-resistant multiple myeloma cells.
    Article Snippet: Gene expression was quantified by quantitative real-time PCR (qPCR) using TaqMan assays from Applied Biosystems (Foster City, CA, USA), according to the manufacturer's instructions. .. The levels of the tumor biomarker IgG2B (Invitrogen; Cat#88-50430-88), a paraprotein produced by 5TGM1 cells, or human lambda light chain (Bethyl Laboratories, Montgomery, TX, USA; Cat#E88-116), were used to determine tumor growth/burden in vivo (serum) or ex vivo (conditioned media). ..

    Incubation:

    Article Title: The oligosaccharyltransferase complex is an essential component of multiple myeloma plasma cells
    Article Snippet: MYC and 523 GADPH mRNAs were detected by real-time PCR (QuantStudio 3, Applied Biosystems, USA). .. 524 525 Human lambda light chain ELISA 526 The Nunc 96-well plates (Thermo Scientific) were coated with goat anti-human lambda chain 527 antibody (20 μg/mL) (Southern Biotechnology, #2070-01) in coating buffer (Human Lambda 528 ELISA Kit, Bethyl Laboratories, #E88116) and incubated overnight at 4°C. ..

    Article Title: The oligosaccharyltransferase complex is an essential component of multiple myeloma plasma cells
    Article Snippet: MYC and GADPH mRNAs were detected by real-time PCR (QuantStudio 3, Applied Biosystems, USA). .. The Nunc 96-well plates (Thermo Scientific) were coated with goat anti-human lambda chain antibody (20 μg/mL) (Southern Biotechnology, #2070-01) in coating buffer (Human Lambda ELISA Kit, Bethyl Laboratories, #E88116) and incubated overnight at 4°C. ..



    Similar Products

    93
    Bethyl intracellular lambda light chain levels
    Intracellular Lambda Light Chain Levels, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lambda+light+chain+kit/Human+Lambda+ELISA+Kit/pm41861438-243-18-30
    Average 93 stars, based on 1 article reviews
    intracellular lambda light chain levels - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Bethyl human lambda light chain
    Human Lambda Light Chain, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lambda+light+chain+kit/Human+Lambda+ELISA+Kit/pm41486357-101-16-20
    Average 93 stars, based on 1 article reviews
    human lambda light chain - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Biorbyt enzyme linked immunosorbent assay elisa kits
    Enzyme Linked Immunosorbent Assay Elisa Kits, supplied by Biorbyt, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lambda+light+chain+kit/Goat+IgG+Against+Human+Ig+Lambda+Light+Chain+(TRITC)/pm41002417-58-15-24
    Average 93 stars, based on 1 article reviews
    enzyme linked immunosorbent assay elisa kits - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    BioVendor Instruments lambda elisa kit
    Lambda Elisa Kit, supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lambda+light+chain+kit/Immunoglobulin+Free+Light+Chains+Kappa+and+Lambda+Human+ELISA/pm40915391-108-16-19
    Average 93 stars, based on 1 article reviews
    lambda elisa kit - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Bethyl human lambda light chain elisa kit
    Human Lambda Light Chain Elisa Kit, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lambda+light+chain+kit/Human+Lambda+ELISA+Kit/pm40043324-383-15-21
    Average 93 stars, based on 1 article reviews
    human lambda light chain elisa kit - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Bethyl human lambda light chain kit
    Human Lambda Light Chain Kit, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lambda+light+chain+kit/Human+Lambda+ELISA+Kit/us11033560-269-9-14
    Average 93 stars, based on 1 article reviews
    human lambda light chain kit - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Bethyl lambda light chain elisa human lambda light chain kit
    RPMI-8226 cells were incubated for 48 hours in the presence or absence of lovastatin (Lov, 10 μM) or varying concentrations of the test compounds. A) Intracellular <t>lambda</t> light chain concentrations were determined via <t>ELISA.</t> Data are expressed as a percentage of control (mean + SD, n=3). The * denotes p < 0.05 per unpaired two-tailed t-test. B) Immunoblot analysis of Rap1a (antibody detects only unmodified protein) and β-tubulin (as a loading control).
    Lambda Light Chain Elisa Human Lambda Light Chain Kit, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lambda+light+chain+kit/Human+Lambda+ELISA+Kit/pmc05752576-198-1-10
    Average 93 stars, based on 1 article reviews
    lambda light chain elisa human lambda light chain kit - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    Image Search Results


    RPMI-8226 cells were incubated for 48 hours in the presence or absence of lovastatin (Lov, 10 μM) or varying concentrations of the test compounds. A) Intracellular lambda light chain concentrations were determined via ELISA. Data are expressed as a percentage of control (mean + SD, n=3). The * denotes p < 0.05 per unpaired two-tailed t-test. B) Immunoblot analysis of Rap1a (antibody detects only unmodified protein) and β-tubulin (as a loading control).

    Journal: Bioorganic & medicinal chemistry

    Article Title: α-Methylation Enhances the Potency of Isoprenoid Triazole Bisphosphonates as Geranylgeranyl Diphosphate Synthase Inhibitors

    doi: 10.1016/j.bmc.2017.10.023

    Figure Lengend Snippet: RPMI-8226 cells were incubated for 48 hours in the presence or absence of lovastatin (Lov, 10 μM) or varying concentrations of the test compounds. A) Intracellular lambda light chain concentrations were determined via ELISA. Data are expressed as a percentage of control (mean + SD, n=3). The * denotes p < 0.05 per unpaired two-tailed t-test. B) Immunoblot analysis of Rap1a (antibody detects only unmodified protein) and β-tubulin (as a loading control).

    Article Snippet: 5.23 Lambda light chain ELISA Human lambda light chain kit (Bethyl Laboratories, Montgomery, TX) was used to quantify intracellular monoclonal protein levels of whole cell lysate.

    Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Control, Two Tailed Test, Western Blot

    RPMI-8226 cells were incubated for 48 hours in the presence or absence of lovastatin (Lov, 10 μM) or varying concentrations of the test compound. A) Intracellular lambda light chain concentrations were determined via ELISA. Data are expressed as a percentage of control (mean + SD, n=3). The * denotes p < 0.05 per unpaired two-tailed t-test. B) Immunoblot analysis of Rap1a (antibody detects only unmodified protein) and β-tubulin (as a loading control).

    Journal: Bioorganic & medicinal chemistry

    Article Title: α-Methylation Enhances the Potency of Isoprenoid Triazole Bisphosphonates as Geranylgeranyl Diphosphate Synthase Inhibitors

    doi: 10.1016/j.bmc.2017.10.023

    Figure Lengend Snippet: RPMI-8226 cells were incubated for 48 hours in the presence or absence of lovastatin (Lov, 10 μM) or varying concentrations of the test compound. A) Intracellular lambda light chain concentrations were determined via ELISA. Data are expressed as a percentage of control (mean + SD, n=3). The * denotes p < 0.05 per unpaired two-tailed t-test. B) Immunoblot analysis of Rap1a (antibody detects only unmodified protein) and β-tubulin (as a loading control).

    Article Snippet: 5.23 Lambda light chain ELISA Human lambda light chain kit (Bethyl Laboratories, Montgomery, TX) was used to quantify intracellular monoclonal protein levels of whole cell lysate.

    Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Control, Two Tailed Test, Western Blot

    RPMI-8226 cells were incubated for 48 hours in the presence or absence of lovastatin (Lov, 10 μM) or varying concentrations of the test compounds. A) Intracellular lambda light chain concentrations were determined via ELISA. Data are expressed as a percentage of control (mean + SD, n=3). The * denotes p < 0.05 per unpaired two-tailed t-test. B) Immunoblot analysis of Rap1a (antibody detects only unmodified protein) and β-tubulin (as a loading control).

    Journal: Bioorganic & medicinal chemistry

    Article Title: α-Methylation Enhances the Potency of Isoprenoid Triazole Bisphosphonates as Geranylgeranyl Diphosphate Synthase Inhibitors

    doi: 10.1016/j.bmc.2017.10.023

    Figure Lengend Snippet: RPMI-8226 cells were incubated for 48 hours in the presence or absence of lovastatin (Lov, 10 μM) or varying concentrations of the test compounds. A) Intracellular lambda light chain concentrations were determined via ELISA. Data are expressed as a percentage of control (mean + SD, n=3). The * denotes p < 0.05 per unpaired two-tailed t-test. B) Immunoblot analysis of Rap1a (antibody detects only unmodified protein) and β-tubulin (as a loading control).

    Article Snippet: 5.23 Lambda light chain ELISA Human lambda light chain kit (Bethyl Laboratories, Montgomery, TX) was used to quantify intracellular monoclonal protein levels of whole cell lysate.

    Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Control, Two Tailed Test, Western Blot

    RPMI-8226 cells were incubated for 48 hours in the presence or absence of lovastatin (Lov, 10 μM) or varying concentrations of the test compounds. A) Intracellular lambda light chain concentrations were determined via ELISA. Data are expressed as a percentage of control (mean + SD, n=3). The * denotes p < 0.05 per unpaired two-tailed t-test. B) Immunoblot analysis of Rap1a (antibody detects only unmodified protein) and β-tubulin (as a loading control).

    Journal: Bioorganic & medicinal chemistry

    Article Title: α-Methylation Enhances the Potency of Isoprenoid Triazole Bisphosphonates as Geranylgeranyl Diphosphate Synthase Inhibitors

    doi: 10.1016/j.bmc.2017.10.023

    Figure Lengend Snippet: RPMI-8226 cells were incubated for 48 hours in the presence or absence of lovastatin (Lov, 10 μM) or varying concentrations of the test compounds. A) Intracellular lambda light chain concentrations were determined via ELISA. Data are expressed as a percentage of control (mean + SD, n=3). The * denotes p < 0.05 per unpaired two-tailed t-test. B) Immunoblot analysis of Rap1a (antibody detects only unmodified protein) and β-tubulin (as a loading control).

    Article Snippet: 5.23 Lambda light chain ELISA Human lambda light chain kit (Bethyl Laboratories, Montgomery, TX) was used to quantify intracellular monoclonal protein levels of whole cell lysate.

    Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Control, Two Tailed Test, Western Blot