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human lambda elisa quantitation  (Fortis Life Sciences)


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    Fortis Life Sciences human lambda elisa quantitation
    Human Lambda Elisa Quantitation, supplied by Fortis Life Sciences, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lambda+elisa+quantitation/Human+Lambda+Free+Light+Chain/pm36170375-330-22-21
    Average 93 stars, based on 36 article reviews
    human lambda elisa quantitation - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: Anti-lambda myeloma antigen (LMA) binding proteins to treat LMA-expressing cancer and autoimmune disorders
    Article Snippet: .. Similarly, human anti-LMA antibodies (6A1, 13H3 and 4A1) demonstrated high and selective affinity to human multiple myeloma cell lines expressing lambda-light chain isotypes and not to kappa-light chain expressing JJN3 cell line (FIG. 4) while clones 18E8, 7F11 and 18F9 demonstrated selective affinity to commercial lambda-light chain (Bethyl) (FIG. 2B). ..

    Clone Assay:

    Article Title: Anti-lambda myeloma antigen (LMA) binding proteins to treat LMA-expressing cancer and autoimmune disorders
    Article Snippet: .. Similarly, human anti-LMA antibodies (6A1, 13H3 and 4A1) demonstrated high and selective affinity to human multiple myeloma cell lines expressing lambda-light chain isotypes and not to kappa-light chain expressing JJN3 cell line (FIG. 4) while clones 18E8, 7F11 and 18F9 demonstrated selective affinity to commercial lambda-light chain (Bethyl) (FIG. 2B). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Heterohybridomas producing human immunoglobulin light chains using CD138-selected bone marrow cells.
    Article Snippet: Background: Light chain research is hampered by lack of mammalian cell lines producing human light chains (FLC).. Therefore, we used heterohybridoma (HH) technology to produce clones making FLC thereby providing tools to study light chain behavior.. Methods: Marrow CD138+ cells from patients with multiple myeloma (MM) and polyclonal gammopathy (PG) were selected, fused with B5-6 T cells and cultured in hypoxanthine-aminopterin-thymidine medium (HAT).

    Article Title: Treatment of al amyloidosis with melflufen
    Article Snippet: .. Levels of kappa and lambda light chains as well as BCMA from the serum was analysed on days 9 and 16 post tumor inoculation using commercial ELISA kits (below) and manufacturer's protocols: Human Kappa ELISA (Bethyl Laboratories, #E80-115) Human Lambda ELISA (Bethyl Laboratories, #E80-116) Human BCMA ELISA (R&D Systems, #DY193). ..

    Article Title: Therapeutic implications of mitochondrial stress-induced proteasome inhibitor resistance in multiple myeloma.
    Article Snippet: .. Lysates of the cell pellets were prepared in RIPA buffer, and the lambda and kappa light chain antibodies were measured using Bethyl Human Lambda ELISA Quantitation Set (E80-116) and Bethyl Human Kappa ELISA Quantitation Set (#E80-115) following the manufacturer’s protocol. .. Bacterial clones containing ATF4 short hairpin RNAs were obtained from Sigma-Aldrich (TRCN0000013573 and TRCN0000013574) and plated on LB agar plates containing ampicillin.

    Article Title: Heterohybridomas producing human immunoglobulin light chains using CD138-selected bone marrow cells
    Article Snippet: .. Heterohybridoma supernatants for ELISA were evaluated in quantitative sandwich ELISA for human IgA, IgG, IgM, κ and λ light chains (Bethyl Laboratories, Montgomery, TX) according to the manufacturers’ instructions. ..

    Article Title: Therapeutic implications of mitochondrial stress–induced proteasome inhibitor resistance in multiple myeloma
    Article Snippet: .. Lysates of the cell pellets were prepared in RIPA buffer, and the lambda and kappa light chain antibodies were measured using Bethyl Human Lambda ELISA Quantitation Set (E80-116) and Bethyl Human Kappa ELISA Quantitation Set (#E80-115) following the manufacturer’s protocol. .. Lysates of the cell pellets were prepared in RIPA buffer, and the lambda and kappa light chain antibodies were measured using Bethyl Human Lambda ELISA Quantitation Set (E80-116) and Bethyl Human Kappa ELISA Quantitation Set (#E80-115) following the manufacturer’s protocol.

    Article Title: A non-ligand surrogate agonist antibody that enhances canonical Wnt signaling and bone regeneration
    Article Snippet: .. One week post treatment, mice were anesthetized and scanned by micro-CT. One week post CT scanning, blood and femurs were collected from the mice, and free human Ig-lambda light chains in serum was assessed using a Human Lambda ELISA Kit (Bethyl Laboratories) according to manufacturer’s instructions. .. One week post treatment, mice were anesthetized and scanned by micro-CT. One week post CT scanning, blood and femurs were collected from the mice, and free human Ig-lambda light chains in serum was assessed using a Human Lambda ELISA Kit (Bethyl Laboratories) according to manufacturer’s instructions.

    Sandwich ELISA:

    Article Title: Heterohybridomas producing human immunoglobulin light chains using CD138-selected bone marrow cells.
    Article Snippet: Background: Light chain research is hampered by lack of mammalian cell lines producing human light chains (FLC).. Therefore, we used heterohybridoma (HH) technology to produce clones making FLC thereby providing tools to study light chain behavior.. Methods: Marrow CD138+ cells from patients with multiple myeloma (MM) and polyclonal gammopathy (PG) were selected, fused with B5-6 T cells and cultured in hypoxanthine-aminopterin-thymidine medium (HAT).

    Article Title: Heterohybridomas producing human immunoglobulin light chains using CD138-selected bone marrow cells
    Article Snippet: .. Heterohybridoma supernatants for ELISA were evaluated in quantitative sandwich ELISA for human IgA, IgG, IgM, κ and λ light chains (Bethyl Laboratories, Montgomery, TX) according to the manufacturers’ instructions. ..

    Quantitation Assay:

    Article Title: Therapeutic implications of mitochondrial stress-induced proteasome inhibitor resistance in multiple myeloma.
    Article Snippet: .. Lysates of the cell pellets were prepared in RIPA buffer, and the lambda and kappa light chain antibodies were measured using Bethyl Human Lambda ELISA Quantitation Set (E80-116) and Bethyl Human Kappa ELISA Quantitation Set (#E80-115) following the manufacturer’s protocol. .. Bacterial clones containing ATF4 short hairpin RNAs were obtained from Sigma-Aldrich (TRCN0000013573 and TRCN0000013574) and plated on LB agar plates containing ampicillin.

    Article Title: Therapeutic implications of mitochondrial stress–induced proteasome inhibitor resistance in multiple myeloma
    Article Snippet: .. Lysates of the cell pellets were prepared in RIPA buffer, and the lambda and kappa light chain antibodies were measured using Bethyl Human Lambda ELISA Quantitation Set (E80-116) and Bethyl Human Kappa ELISA Quantitation Set (#E80-115) following the manufacturer’s protocol. .. Lysates of the cell pellets were prepared in RIPA buffer, and the lambda and kappa light chain antibodies were measured using Bethyl Human Lambda ELISA Quantitation Set (E80-116) and Bethyl Human Kappa ELISA Quantitation Set (#E80-115) following the manufacturer’s protocol.

    Micro-CT:

    Article Title: A non-ligand surrogate agonist antibody that enhances canonical Wnt signaling and bone regeneration
    Article Snippet: .. One week post treatment, mice were anesthetized and scanned by micro-CT. One week post CT scanning, blood and femurs were collected from the mice, and free human Ig-lambda light chains in serum was assessed using a Human Lambda ELISA Kit (Bethyl Laboratories) according to manufacturer’s instructions. .. One week post treatment, mice were anesthetized and scanned by micro-CT. One week post CT scanning, blood and femurs were collected from the mice, and free human Ig-lambda light chains in serum was assessed using a Human Lambda ELISA Kit (Bethyl Laboratories) according to manufacturer’s instructions.



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    Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human <t>lambda</t> light chain concentrations correlating with tumor burden were determined by <t>ELISA</t> at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.
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    Image Search Results


    Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human lambda light chain concentrations correlating with tumor burden were determined by ELISA at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.

    Journal: Cell chemical biology

    Article Title: Stable and potent selenomab-drug conjugates

    doi: 10.1016/j.chembiol.2017.02.012

    Figure Lengend Snippet: Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human lambda light chain concentrations correlating with tumor burden were determined by ELISA at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.

    Article Snippet: Tumor burden was monitored (i) by weekly in vivo bioluminescence imaging using a Xenogen IVIS Imaging System (Caliper Life Sciences) following i.p. injection of 100 μL of 25 mg/mL D-luciferin (Caliper Life Sciences) and (ii) by weekly measurement of human lambda light chain concentrations using the Human Lambda ELISA Quantitation Set (Bethyl Laboratories).

    Techniques: Injection, Enzyme-linked Immunosorbent Assay

    (A) Human breast cancer cell line KPL-4 was xenografted into the mammary fat pads of female NSG mice, grown to ~150 mm3, randomized into 5 groups comprising 6 mice each, and treated with i.v. (tail vein) injections of the indicated ADCs and controls four times every week at 5 mg/kg. Mean ± SD values are plotted. (B) Four female CD-1 mice were injected i.v. with 6 mg/kg of anti-HER2 scFv-Fc(Ser396Sec)/CN29. The plasma concentrations of total antibody and intact ADC were quantified by ELISA at the indicated time points, using HER2 ECD for capturing and either anti-His mAb (total antibody) or anti-MMAF mAb (intact ADC) for detection. Shown are mean ± SD values for each time point. See also Figure S5, Figure S6, Figure S7, and Table S6.

    Journal: Cell chemical biology

    Article Title: Stable and potent selenomab-drug conjugates

    doi: 10.1016/j.chembiol.2017.02.012

    Figure Lengend Snippet: (A) Human breast cancer cell line KPL-4 was xenografted into the mammary fat pads of female NSG mice, grown to ~150 mm3, randomized into 5 groups comprising 6 mice each, and treated with i.v. (tail vein) injections of the indicated ADCs and controls four times every week at 5 mg/kg. Mean ± SD values are plotted. (B) Four female CD-1 mice were injected i.v. with 6 mg/kg of anti-HER2 scFv-Fc(Ser396Sec)/CN29. The plasma concentrations of total antibody and intact ADC were quantified by ELISA at the indicated time points, using HER2 ECD for capturing and either anti-His mAb (total antibody) or anti-MMAF mAb (intact ADC) for detection. Shown are mean ± SD values for each time point. See also Figure S5, Figure S6, Figure S7, and Table S6.

    Article Snippet: Tumor burden was monitored (i) by weekly in vivo bioluminescence imaging using a Xenogen IVIS Imaging System (Caliper Life Sciences) following i.p. injection of 100 μL of 25 mg/mL D-luciferin (Caliper Life Sciences) and (ii) by weekly measurement of human lambda light chain concentrations using the Human Lambda ELISA Quantitation Set (Bethyl Laboratories).

    Techniques: Injection, Clinical Proteomics, Enzyme-linked Immunosorbent Assay

    KEY RESOURCES TABLE

    Journal: Cell chemical biology

    Article Title: Stable and potent selenomab-drug conjugates

    doi: 10.1016/j.chembiol.2017.02.012

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Tumor burden was monitored (i) by weekly in vivo bioluminescence imaging using a Xenogen IVIS Imaging System (Caliper Life Sciences) following i.p. injection of 100 μL of 25 mg/mL D-luciferin (Caliper Life Sciences) and (ii) by weekly measurement of human lambda light chain concentrations using the Human Lambda ELISA Quantitation Set (Bethyl Laboratories).

    Techniques: Virus, Recombinant, Marker, Conjugation Assay, Proliferation Assay, Enzyme-linked Immunosorbent Assay, Quantitation Assay, Software