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gene exp ifnl1 hs00601677 g1  (Thermo Fisher)


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    Thermo Fisher gene exp ifnl1 hs00601677 g1
    Gene Exp Ifnl1 Hs00601677 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lambda+elisa+quantitation+set/Gene+Exp%2E+IFNL1%2C+Hs00601677_g1/pmc11602746-55-53-39
    Average 99 stars, based on 1 article reviews
    gene exp ifnl1 hs00601677 g1 - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    other:

    Article Title: OAS1 and OAS3 genetic variants enhance inflammatory responses to SARS-CoV-2
    Article Snippet: At 24 and 48 hpt, the cells were lysed in passive lysis buffer (Promega) and clarified by centrifugation.

    Article Title: Viral infection and brain inflammation with seizures in PARK7 deficiency
    Article Snippet: After 3 days, media was removed, and cells were stained using crystal violet stain (1:2:7 vol/vol/vol, crystal violet solution [Sigma-Aldrich], absolute ethanol, and deionized H 2 O); plaques were counted using a standard light microscope, and the PFU titer was calculated by the following formula: #plaques × dilution factor virus stock/volume virus added = PFU/ml.

    Article Title: Intrinsic OASL expression governs heterogeneity in interferon induction during influenza A virus infection.
    Article Snippet: Donors 3 and 4 were run on NovaSeq X (Illumina) and NovaSeq X Plus (Illumina) respectively, both using 2 × 150 nt PE reads.

    TaqMan Assay:

    Article Title: Dissecting placental host-pathogen interactions: Rift Valley fever virus infection in early human trophoblast stem cells
    Article Snippet: TaqMan Assay: IFN-β1 , Thermo Scientific , Assay ID: Hs01077958_s1. .. TaqMan Assay: IFN-λ , Thermo Scientific , Assay ID: Hs00601677_g1. .. TaqMan Assay: IL-6 , Thermo Scientific , Assay ID: Hs00985639_m1.

    Article Title: Dissecting placental host-pathogen interactions: Rift Valley fever virus infection in early human trophoblast stem cells
    Article Snippet: Each reaction was performed in a 20 μL volume using 523 a 1:10 dilution of cDNA synthesized previously and run for 40 cycles under the default 524 thermal cycling conditions recommended for the TaqMan Fast Advanced Master Mix 525 (Thermo Scientific). .. TaqMan FAM/MGB probe assays (Thermo Scientific) were employed 526 for detection. mRNA expression levels were assessed for the following cytokines using 527 the corresponding TaqMan Assay IDs: IFNA1 (Hs00256882_s1), IFNB1 528 (Hs01077958_s1), IFNL1 (Hs00601677_g1), IL6 (Hs00985639_m1), IL8 529 (Hs00174103_m1), and TNFA (Hs00174128_m1). ..

    Article Title: Dissecting placental host-pathogen interactions: Rift Valley fever virus infection in early human trophoblast stem cells
    Article Snippet: Each reaction was performed in a 20 μL volume using a 1:10 dilution of cDNA synthesized previously and run for 40 cycles under the default thermal cycling conditions recommended for the TaqMan Fast Advanced Master Mix (Thermo Scientific). .. TaqMan FAM/MGB probe assays (Thermo Scientific) were employed for detection. mRNA expression levels were assessed for the following cytokines using the corresponding TaqMan Assay IDs: IFNA1 (Hs00256882_s1), IFNB1 (Hs01077958_s1), IFNL1 (Hs00601677_g1), IL6 (Hs00985639_m1), IL8 (Hs00174103_m1), and TNFA (Hs00174128_m1). ..

    Quantitative RT-PCR:

    Article Title: Recurrent severe viral infection in a child with inherited complete TBK1 deficiency
    Article Snippet: The RNA was reverse-transcribed with the Super Script IV VILO (SSIV VILO) Master Mix (11756050; Thermo Fisher Scientific) according to the manufacturer’s instructions. .. RT-qPCR was performed with Applied Biosystems TaqMan assays for TBK1 (HS00179410_m1), IFNB1 (Hs01077958_s1), IFNL1 (Hs00601677_g1), IFIT1 (Hs00356631_g1), IL6 (Hs00174131_m1), and the β-glucuronidase (GUS; #4310888E) housekeeping gene for normalization. ..

    Real-time Polymerase Chain Reaction:

    Article Title: OAS1 and OAS3 genetic variants enhance inflammatory responses to SARS-CoV-2
    Article Snippet: Knock-down experiments: non-targeting (NT) negative control siRNA , Thermo Fisher Scientific , Cat# AM4635. .. qPCR: human IFNL1 (Hs00601677_g1), human TNF (Hs00174128_m1), human CXCL10 (Hs00171042_m1), human CCL2 (Hs00234140_m1), human IL6 (Hs 00174131_m1), human OAS1 (Hs00973635_m1), human OAS3 (Hs00196324_m1), human GAPDH (Hs02786624_g1) , Thermo Fisher Scientific , Cat#4331182. .. qPCR: murine Ccl2 (Mm00441242_m1), murine Cxcl10 (Mm00445235_m1), murine Tnf (Mm00443258_m1), murine Ccl8 (Mm01297183_m1), murine Oas3 (Mm00460944_m1), murine Hprt1 (Mm00446968_m1) , Thermo Fisher Scientific , Cat#4331182.

    Expressing:

    Article Title: Dissecting placental host-pathogen interactions: Rift Valley fever virus infection in early human trophoblast stem cells
    Article Snippet: Each reaction was performed in a 20 μL volume using 523 a 1:10 dilution of cDNA synthesized previously and run for 40 cycles under the default 524 thermal cycling conditions recommended for the TaqMan Fast Advanced Master Mix 525 (Thermo Scientific). .. TaqMan FAM/MGB probe assays (Thermo Scientific) were employed 526 for detection. mRNA expression levels were assessed for the following cytokines using 527 the corresponding TaqMan Assay IDs: IFNA1 (Hs00256882_s1), IFNB1 528 (Hs01077958_s1), IFNL1 (Hs00601677_g1), IL6 (Hs00985639_m1), IL8 529 (Hs00174103_m1), and TNFA (Hs00174128_m1). ..

    Article Title: Dissecting placental host-pathogen interactions: Rift Valley fever virus infection in early human trophoblast stem cells
    Article Snippet: Each reaction was performed in a 20 μL volume using a 1:10 dilution of cDNA synthesized previously and run for 40 cycles under the default thermal cycling conditions recommended for the TaqMan Fast Advanced Master Mix (Thermo Scientific). .. TaqMan FAM/MGB probe assays (Thermo Scientific) were employed for detection. mRNA expression levels were assessed for the following cytokines using the corresponding TaqMan Assay IDs: IFNA1 (Hs00256882_s1), IFNB1 (Hs01077958_s1), IFNL1 (Hs00601677_g1), IL6 (Hs00985639_m1), IL8 (Hs00174103_m1), and TNFA (Hs00174128_m1). ..



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    Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human <t>lambda</t> light chain concentrations correlating with tumor burden were determined by <t>ELISA</t> at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.
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    Image Search Results


    Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human lambda light chain concentrations correlating with tumor burden were determined by ELISA at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.

    Journal: Cell chemical biology

    Article Title: Stable and potent selenomab-drug conjugates

    doi: 10.1016/j.chembiol.2017.02.012

    Figure Lengend Snippet: Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human lambda light chain concentrations correlating with tumor burden were determined by ELISA at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.

    Article Snippet: Human Lambda ELISA Quantitation Set , Bethyl Laboratories , Cat#E80-116.

    Techniques: Injection, Enzyme-linked Immunosorbent Assay

    (A) Human breast cancer cell line KPL-4 was xenografted into the mammary fat pads of female NSG mice, grown to ~150 mm3, randomized into 5 groups comprising 6 mice each, and treated with i.v. (tail vein) injections of the indicated ADCs and controls four times every week at 5 mg/kg. Mean ± SD values are plotted. (B) Four female CD-1 mice were injected i.v. with 6 mg/kg of anti-HER2 scFv-Fc(Ser396Sec)/CN29. The plasma concentrations of total antibody and intact ADC were quantified by ELISA at the indicated time points, using HER2 ECD for capturing and either anti-His mAb (total antibody) or anti-MMAF mAb (intact ADC) for detection. Shown are mean ± SD values for each time point. See also Figure S5, Figure S6, Figure S7, and Table S6.

    Journal: Cell chemical biology

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    doi: 10.1016/j.chembiol.2017.02.012

    Figure Lengend Snippet: (A) Human breast cancer cell line KPL-4 was xenografted into the mammary fat pads of female NSG mice, grown to ~150 mm3, randomized into 5 groups comprising 6 mice each, and treated with i.v. (tail vein) injections of the indicated ADCs and controls four times every week at 5 mg/kg. Mean ± SD values are plotted. (B) Four female CD-1 mice were injected i.v. with 6 mg/kg of anti-HER2 scFv-Fc(Ser396Sec)/CN29. The plasma concentrations of total antibody and intact ADC were quantified by ELISA at the indicated time points, using HER2 ECD for capturing and either anti-His mAb (total antibody) or anti-MMAF mAb (intact ADC) for detection. Shown are mean ± SD values for each time point. See also Figure S5, Figure S6, Figure S7, and Table S6.

    Article Snippet: Human Lambda ELISA Quantitation Set , Bethyl Laboratories , Cat#E80-116.

    Techniques: Injection, Clinical Proteomics, Enzyme-linked Immunosorbent Assay

    KEY RESOURCES TABLE

    Journal: Cell chemical biology

    Article Title: Stable and potent selenomab-drug conjugates

    doi: 10.1016/j.chembiol.2017.02.012

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Human Lambda ELISA Quantitation Set , Bethyl Laboratories , Cat#E80-116.

    Techniques: Virus, Recombinant, Marker, Conjugation Assay, Proliferation Assay, Enzyme-linked Immunosorbent Assay, Quantitation Assay, Software