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human lambda elisa quantitation set  (Bethyl)


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    Bethyl human lambda elisa quantitation set
    Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human <t>lambda</t> light chain concentrations correlating with tumor burden were determined by <t>ELISA</t> at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.
    Human Lambda Elisa Quantitation Set, supplied by Bethyl, used in various techniques. Bioz Stars score: 96/100, based on 544 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lambda+elisa+quantitation+set/Human+Albumin+ELISA+Quantitation+Set/pmc05400723-52-0-6
    Average 96 stars, based on 544 article reviews
    human lambda elisa quantitation set - by Bioz Stars, 2026-11
    96/100 stars

    Images

    1) Product Images from "Stable and potent selenomab-drug conjugates"

    Article Title: Stable and potent selenomab-drug conjugates

    Journal: Cell chemical biology

    doi: 10.1016/j.chembiol.2017.02.012

    Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human lambda light chain concentrations correlating with tumor burden were determined by ELISA at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.
    Figure Legend Snippet: Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human lambda light chain concentrations correlating with tumor burden were determined by ELISA at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.

    Techniques Used: Injection, Enzyme-linked Immunosorbent Assay

    (A) Human breast cancer cell line KPL-4 was xenografted into the mammary fat pads of female NSG mice, grown to ~150 mm3, randomized into 5 groups comprising 6 mice each, and treated with i.v. (tail vein) injections of the indicated ADCs and controls four times every week at 5 mg/kg. Mean ± SD values are plotted. (B) Four female CD-1 mice were injected i.v. with 6 mg/kg of anti-HER2 scFv-Fc(Ser396Sec)/CN29. The plasma concentrations of total antibody and intact ADC were quantified by ELISA at the indicated time points, using HER2 ECD for capturing and either anti-His mAb (total antibody) or anti-MMAF mAb (intact ADC) for detection. Shown are mean ± SD values for each time point. See also Figure S5, Figure S6, Figure S7, and Table S6.
    Figure Legend Snippet: (A) Human breast cancer cell line KPL-4 was xenografted into the mammary fat pads of female NSG mice, grown to ~150 mm3, randomized into 5 groups comprising 6 mice each, and treated with i.v. (tail vein) injections of the indicated ADCs and controls four times every week at 5 mg/kg. Mean ± SD values are plotted. (B) Four female CD-1 mice were injected i.v. with 6 mg/kg of anti-HER2 scFv-Fc(Ser396Sec)/CN29. The plasma concentrations of total antibody and intact ADC were quantified by ELISA at the indicated time points, using HER2 ECD for capturing and either anti-His mAb (total antibody) or anti-MMAF mAb (intact ADC) for detection. Shown are mean ± SD values for each time point. See also Figure S5, Figure S6, Figure S7, and Table S6.

    Techniques Used: Injection, Clinical Proteomics, Enzyme-linked Immunosorbent Assay

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Virus, Recombinant, Marker, Conjugation Assay, Proliferation Assay, Enzyme-linked Immunosorbent Assay, Quantitation Assay, Software

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: A clinically defined and xeno-free hydrogel system for regenerative medicine
    Article Snippet: .. Albumin concentrations in supernatants were measured using a human albumin ELISA quantification kit (Bethyl Lab, no. E80-129). ..

    Article Title: In vitro space of Disse model for exploration of drug induced hepatotoxicity.
    Article Snippet: .. Specific ELISA kits were used according to the manufacturer's instructions: Human Albumin ELISA Quantification Set, Cat N E80129, Bethyl Laboratories and Urea Assay kit, Sigma Aldrich, MAK006. ..

    Article Title: Liver-on-Chip: An Analysis of Liver Cell Types, Seeding Parameters, and Liver Function Assays
    Article Snippet: 4 , HepG2, Caco-2 , 200 μL of 5.5 × 10 6 –10 7 cells/mL (per cell line) , 1000 μL of cell lysate added to the collected cells (4 °C, 30 min), centrifuged at 2000 rpm for 20 min, and then collected , HepG2: 12.5–16.9 mg/mL HepG2 + Caco-2: 17.1–18.5 mg/mL , NC , NA , NA , 1, 3, 5 and 7 days , Human Albumin ELISA Kit Fankew , NA , NA , NA , [ ] . .. 5 , HepG2, HUVEC , Small modules: ~8.0 × 10 7 cells/cm 3 , Large modules: ~1.5 × 10 7 cells/cm 3 1 × 10 6 to 1 × 10 7 cells/mL, 1.5 × 10 7 cells/mL was useable. , Ten modules transferred to a 24 well-plate in 500 μL of fresh medium , Average: 14 μg/10 6 cell/day 0.012–0.0176 ng/cell/day for both small and large module. , 12–17.6 (avg. 14) , NA , NA , Days 3 and 7 evaluations , Human Albumin ELISA Quantitation Kit (Bethyl Laboratories Inc.). , NA , NA , NA , [ ] . .. 6 , HepG2, HUVEC-T1, THP-1, HHSC, Caco-2, HT29-MTX-E12 , 100 cells/spheroid , 10 μL from each side , Co-culture (liver chamber) 100–400 mg/L 3D liver Spheroid: 2–400 mg/mL day 2, 7, 14 , NC , Co-culture (liver chamber) 4–6 mM 3D liver Spheroid: 110–175 nmol day 2, 7, 14 , NC , Days 7, 10, 14 and 21 , Human Albumin ELISA Kit (Abcam), , Urea Assay Kit (Abcam), , 0.8 mL/min , NA , [ ] .

    Article Title: Compositions and methods for long term culture of hepatocytes
    Article Snippet: .. Unsupervised hierarchical clustering of RNA-Seq data was generated by hclust package in R. Biochemical Assays Human albumin was measured using the Human Albumin ELISA Quantitation kit (Bethyl Laboratories). .. Urea concentration was measured by the QuantiChromTM Urea Assay Kit (BioAssay Systems).

    Article Title: MicroRNA-30c analog C2 decreases plasma cholesterol and atherosclerosis without causing liver injury in preclinical studies.
    Article Snippet: The mice were maintained on Pico Lab High Energy Mouse diet 5LJ5 (LabDiet, Inc) for ~21 weeks posttransplantation. .. Liver humanization was determined by human albumin (hALB) measurement in whole blood with a human-specific albumin antibody ELISA (Bethyl Laboratories Inc, E80-129). ..

    other:

    Article Title: Functional evaluation of a natural AAV capsid liver targeting motif in human hepatocytes
    Article Snippet: Human serum albumin (HSA) was quantified by ELISA (Bethyl Labs #E80-129).

    Article Title: Engineered hepatic cells with stable expansion and heat-inducible liver function.
    Article Snippet: Albumin production was quantified by collecting culture supernatants at 24 h after the final medium change and measuring albumin concentration by ELISA using a commercial kit (E80-129; Bethyl Laboratories, Montgomery, TX, USA), in accordance with the manufacturer’s instructions.

    Quantitation Assay:

    Article Title: Liver-on-Chip: An Analysis of Liver Cell Types, Seeding Parameters, and Liver Function Assays
    Article Snippet: 4 , HepG2, Caco-2 , 200 μL of 5.5 × 10 6 –10 7 cells/mL (per cell line) , 1000 μL of cell lysate added to the collected cells (4 °C, 30 min), centrifuged at 2000 rpm for 20 min, and then collected , HepG2: 12.5–16.9 mg/mL HepG2 + Caco-2: 17.1–18.5 mg/mL , NC , NA , NA , 1, 3, 5 and 7 days , Human Albumin ELISA Kit Fankew , NA , NA , NA , [ ] . .. 5 , HepG2, HUVEC , Small modules: ~8.0 × 10 7 cells/cm 3 , Large modules: ~1.5 × 10 7 cells/cm 3 1 × 10 6 to 1 × 10 7 cells/mL, 1.5 × 10 7 cells/mL was useable. , Ten modules transferred to a 24 well-plate in 500 μL of fresh medium , Average: 14 μg/10 6 cell/day 0.012–0.0176 ng/cell/day for both small and large module. , 12–17.6 (avg. 14) , NA , NA , Days 3 and 7 evaluations , Human Albumin ELISA Quantitation Kit (Bethyl Laboratories Inc.). , NA , NA , NA , [ ] . .. 6 , HepG2, HUVEC-T1, THP-1, HHSC, Caco-2, HT29-MTX-E12 , 100 cells/spheroid , 10 μL from each side , Co-culture (liver chamber) 100–400 mg/L 3D liver Spheroid: 2–400 mg/mL day 2, 7, 14 , NC , Co-culture (liver chamber) 4–6 mM 3D liver Spheroid: 110–175 nmol day 2, 7, 14 , NC , Days 7, 10, 14 and 21 , Human Albumin ELISA Kit (Abcam), , Urea Assay Kit (Abcam), , 0.8 mL/min , NA , [ ] .

    Article Title: Compositions and methods for long term culture of hepatocytes
    Article Snippet: .. Unsupervised hierarchical clustering of RNA-Seq data was generated by hclust package in R. Biochemical Assays Human albumin was measured using the Human Albumin ELISA Quantitation kit (Bethyl Laboratories). .. Urea concentration was measured by the QuantiChromTM Urea Assay Kit (BioAssay Systems).

    RNA sequencing:

    Article Title: Compositions and methods for long term culture of hepatocytes
    Article Snippet: .. Unsupervised hierarchical clustering of RNA-Seq data was generated by hclust package in R. Biochemical Assays Human albumin was measured using the Human Albumin ELISA Quantitation kit (Bethyl Laboratories). .. Urea concentration was measured by the QuantiChromTM Urea Assay Kit (BioAssay Systems).

    Generated:

    Article Title: Compositions and methods for long term culture of hepatocytes
    Article Snippet: .. Unsupervised hierarchical clustering of RNA-Seq data was generated by hclust package in R. Biochemical Assays Human albumin was measured using the Human Albumin ELISA Quantitation kit (Bethyl Laboratories). .. Urea concentration was measured by the QuantiChromTM Urea Assay Kit (BioAssay Systems).

    Sandwich ELISA:

    Article Title: Orthogonal CRISPR screens and human liver chimeric mice identify hepatitis B virus host factors
    Article Snippet: .. Levels of hAlb were quantified using a sandwich ELISA assay (Bethyl Laboratories) to determine the extent of human hepatocyte repopulation, as serum hAlb levels correlate with liver humanization in FNRG mice. ..



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    Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human <t>lambda</t> light chain concentrations correlating with tumor burden were determined by <t>ELISA</t> at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.
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    Image Search Results


    Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human lambda light chain concentrations correlating with tumor burden were determined by ELISA at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.

    Journal: Cell chemical biology

    Article Title: Stable and potent selenomab-drug conjugates

    doi: 10.1016/j.chembiol.2017.02.012

    Figure Lengend Snippet: Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human lambda light chain concentrations correlating with tumor burden were determined by ELISA at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.

    Article Snippet: Human Lambda ELISA Quantitation Set , Bethyl Laboratories , Cat#E80-116.

    Techniques: Injection, Enzyme-linked Immunosorbent Assay

    (A) Human breast cancer cell line KPL-4 was xenografted into the mammary fat pads of female NSG mice, grown to ~150 mm3, randomized into 5 groups comprising 6 mice each, and treated with i.v. (tail vein) injections of the indicated ADCs and controls four times every week at 5 mg/kg. Mean ± SD values are plotted. (B) Four female CD-1 mice were injected i.v. with 6 mg/kg of anti-HER2 scFv-Fc(Ser396Sec)/CN29. The plasma concentrations of total antibody and intact ADC were quantified by ELISA at the indicated time points, using HER2 ECD for capturing and either anti-His mAb (total antibody) or anti-MMAF mAb (intact ADC) for detection. Shown are mean ± SD values for each time point. See also Figure S5, Figure S6, Figure S7, and Table S6.

    Journal: Cell chemical biology

    Article Title: Stable and potent selenomab-drug conjugates

    doi: 10.1016/j.chembiol.2017.02.012

    Figure Lengend Snippet: (A) Human breast cancer cell line KPL-4 was xenografted into the mammary fat pads of female NSG mice, grown to ~150 mm3, randomized into 5 groups comprising 6 mice each, and treated with i.v. (tail vein) injections of the indicated ADCs and controls four times every week at 5 mg/kg. Mean ± SD values are plotted. (B) Four female CD-1 mice were injected i.v. with 6 mg/kg of anti-HER2 scFv-Fc(Ser396Sec)/CN29. The plasma concentrations of total antibody and intact ADC were quantified by ELISA at the indicated time points, using HER2 ECD for capturing and either anti-His mAb (total antibody) or anti-MMAF mAb (intact ADC) for detection. Shown are mean ± SD values for each time point. See also Figure S5, Figure S6, Figure S7, and Table S6.

    Article Snippet: Human Lambda ELISA Quantitation Set , Bethyl Laboratories , Cat#E80-116.

    Techniques: Injection, Clinical Proteomics, Enzyme-linked Immunosorbent Assay

    KEY RESOURCES TABLE

    Journal: Cell chemical biology

    Article Title: Stable and potent selenomab-drug conjugates

    doi: 10.1016/j.chembiol.2017.02.012

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Human Lambda ELISA Quantitation Set , Bethyl Laboratories , Cat#E80-116.

    Techniques: Virus, Recombinant, Marker, Conjugation Assay, Proliferation Assay, Enzyme-linked Immunosorbent Assay, Quantitation Assay, Software