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alexa fluor 488 goat anti human antibody  (Jackson Immuno)


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    Structured Review

    Jackson Immuno alexa fluor 488 goat anti human antibody
    Alexa Fluor 488 Goat Anti Human Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+igg/Alexa+Fluor+488+AffiniPure+Goat+Anti-Human+IgG/pmc12373799-324-5-10
    Average 94 stars, based on 125 article reviews
    alexa fluor 488 goat anti human antibody - by Bioz Stars, 2026-11
    94/100 stars

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    Incubation:

    Article Title: Role of peptidylarginine deiminase 2 in a murine model of traumatic brain injury
    Article Snippet: Following 1 hour incubation with blocking buffer (3% bovine serum albumin) at room temperature, the tissue sections were then incubated overnight at 4°C with primary antibodies against proteins including citrullinated histone 3 (CitH3; 1:200, designed in house, as described previously 21 ), PAD2 (3:200, Protein Tech, 66386-1-Ig), PAD4 (1:200, Protein Tech, 17373-1-AP), caspase-11 (1:200, Abcam, Ab 180673), and glial fibrillatory acidic protein (GFAP; 1:100, Thermo Fisher, 13-0300). .. Thereafter, the sections were incubated with the appropriate secondary antibodies (1:1000, Goat anti-Mouse IgG2b, A21141; 1:1000, Donkey anti-rabbit Jackson ImmunoResearch, 711-545-152; 1:500, Goat anti-human Jackson ImmunoResearch, 109-545-088; and 1:1000, Goat anti-rat Jackson ImmunoResearch, 112-605-003) for 1 hour at room temperature in the dark. .. Following counter-staining with 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher, 815-968-0747), images were acquired with an inverted fluorescence microscope (Olympus, Tokyo, Japan).

    Article Title: Pathogenic human autoantibodies against NMDA receptor alter dendritic spine size.
    Article Snippet: To detect NMDAR-Ab, a cell-based indirect immunofluorescence assay (Autoimmune Encephalitis Mosaic1, Euroimmun, Lübeck, Germany) was performed according to the manufacturer’s instructions. .. Briefly, fixed HEK cells transfected with the relevant antigen on BIOCHIP® slides were sequentially incubated with undiluted CSF and Alexa Fluor 488-conjugated goat anti-human immunoglobulin G (IgG) (1:1,000; Jackson ImmunoResearch, West Grove, PA, USA). .. Fluorescent images were acquired using an AR TIC LE IN PR ES S FV3000 laser scanning confocal microscope (Olympus, Tokyo, Japan).

    Transfection:

    Article Title: Pathogenic human autoantibodies against NMDA receptor alter dendritic spine size.
    Article Snippet: To detect NMDAR-Ab, a cell-based indirect immunofluorescence assay (Autoimmune Encephalitis Mosaic1, Euroimmun, Lübeck, Germany) was performed according to the manufacturer’s instructions. .. Briefly, fixed HEK cells transfected with the relevant antigen on BIOCHIP® slides were sequentially incubated with undiluted CSF and Alexa Fluor 488-conjugated goat anti-human immunoglobulin G (IgG) (1:1,000; Jackson ImmunoResearch, West Grove, PA, USA). .. Fluorescent images were acquired using an AR TIC LE IN PR ES S FV3000 laser scanning confocal microscope (Olympus, Tokyo, Japan).

    Clinical Proteomics:

    Article Title: Pathogenic human autoantibodies against NMDA receptor alter dendritic spine size.
    Article Snippet: To detect NMDAR-Ab, a cell-based indirect immunofluorescence assay (Autoimmune Encephalitis Mosaic1, Euroimmun, Lübeck, Germany) was performed according to the manufacturer’s instructions. .. Briefly, fixed HEK cells transfected with the relevant antigen on BIOCHIP® slides were sequentially incubated with undiluted CSF and Alexa Fluor 488-conjugated goat anti-human immunoglobulin G (IgG) (1:1,000; Jackson ImmunoResearch, West Grove, PA, USA). .. Fluorescent images were acquired using an AR TIC LE IN PR ES S FV3000 laser scanning confocal microscope (Olympus, Tokyo, Japan).

    other:

    Article Title: MYC drives left-handed Z-DNA formation to shape gene expression.
    Article Snippet: Alexa 979 AR TI CL E IN P RE SS ARTICLE IN PRESS Fluor® 488 AffiniPureTM Goat Anti-Human IgG (H+L) (Jackson ImmunoResearch 980 Laboratories, Cat. #109-545-003).

    Article Title: MYC drives left-handed Z-DNA formation to shape gene expression
    Article Snippet: Alexa Fluor® 488 AffiniPureTM Goat Anti-Human IgG (H + L) (Jackson ImmunoResearch Laboratories, Cat. #109-545-003).

    Binding Assay:

    Article Title: Preclinical pharmacology, pharmacokinetics, and pharmacodynamics of veligrotug, a full antagonist antibody to the IGF-1 receptor in development for thyroid eye disease
    Article Snippet: A titration of serially diluted veligrotug, an anti-human IR antibody (Abcam, catalog no. EPR19322 ) or 200 nM IgG1κ isotype control (BioLegend) was then added to the cell suspension and incubated for 30 minutes at 4°C. .. Antibody binding was detected by incubating cells at 4°C for 30 minutes with 2.5 μg/mL Alexa fluor 488-goat Fab anti-human IgG, Fcγ secondary antibody (Jackson Immunoresearch) in 2% FBS, 0.5% NGS and 0.2% sodium azide/PBS. .. A membrane impermeable stain (NucSpot Far-Red; Biotium) was used to detect and exclude necrotic or apoptotic cells.

    Article Title: Preclinical pharmacology, pharmacokinetics, and pharmacodynamics of veligrotug, a full antagonist antibody to the IGF-1 receptor in development for thyroid eye disease
    Article Snippet: A titration of serially diluted veligrotug or 200 nM IgG1κ isotype control (BioLegend, catalog no. 403502; San Diego, CA) in flow cytometry buffer was then added to the cell suspension and incubated for 30 minutes at 4°C. .. Antibody binding was detected by incubating cells at 4°C for 30 minutes with 2.5 μg/mL Alexa fluor 488-goat Fab anti-human IgG, Fcγ secondary antibody (Jackson Immunoresearch, catalog no. 109–847–008) in the BSA-based commercial flow buffer. .. A membrane impermeable stain (NucSpot Far-Red, Biotium; Fremont, CA) was used to detect and exclude necrotic or apoptotic cells.

    Next-Generation Sequencing:

    Article Title: Preclinical pharmacology, pharmacokinetics, and pharmacodynamics of veligrotug, a full antagonist antibody to the IGF-1 receptor in development for thyroid eye disease
    Article Snippet: A titration of serially diluted veligrotug, an anti-human IR antibody (Abcam, catalog no. EPR19322 ) or 200 nM IgG1κ isotype control (BioLegend) was then added to the cell suspension and incubated for 30 minutes at 4°C. .. Antibody binding was detected by incubating cells at 4°C for 30 minutes with 2.5 μg/mL Alexa fluor 488-goat Fab anti-human IgG, Fcγ secondary antibody (Jackson Immunoresearch) in 2% FBS, 0.5% NGS and 0.2% sodium azide/PBS. .. A membrane impermeable stain (NucSpot Far-Red; Biotium) was used to detect and exclude necrotic or apoptotic cells.

    Western Blot:

    Article Title: Chlamydia trachomatis highjacks host MYO1C for actin cage recruitment at the bacterial inclusion.
    Article Snippet: .. Antibodies, reagents and plasmids Antibodies Species Experiment Dilution Reference anti-MYO1C mouse Immunoblotting Immunofluorescence 1:800 1:200 Santa Cruz, sc− 136544 anti-actin mouse Immunoblotting 1:1000 Sigma, A− 2228 anti-clathrin mouse Immunoblotting 1:1000 Santa Cruz, sc− 12734 anti-Myosin II human Immunofluorescence 1:100 Recombinant antibody (continued on next page) (continued ) platform, Institut Curie, Paris, France anti-CT529 rabbit Immunoblotting Immunofluorescence 1:500 1:50 Agathe Subtil, Pasteur Institute, Paris, France Alexa 488- labeled anti-mouseIgG goat Immunofluorescence 1:500 Jackson ImmunoResearch Laboratories Alexa 488- labeled antihuman-IgG goat Immunofluorescence 1:500 Jackson ImmunoResearch Laboratories HRPconjugated anti-rabbitIgG goat Immunoblotting 1:5000 Jackson ImmunoResearch Laboratories HRPconjugated anti-mouseIgG antibodies goat Immunoblotting 1:5000 Jackson ImmunoResearch Laboratories DNA was stained with DAPI (Life Technologies). .. Actin was visualized by incubation with Alexa Fluor 568 Phalloidin (Invitrogen, A12380) in PBS/1 % BSA for 1 h at 37◦C.

    Immunofluorescence:

    Article Title: Chlamydia trachomatis highjacks host MYO1C for actin cage recruitment at the bacterial inclusion.
    Article Snippet: .. Antibodies, reagents and plasmids Antibodies Species Experiment Dilution Reference anti-MYO1C mouse Immunoblotting Immunofluorescence 1:800 1:200 Santa Cruz, sc− 136544 anti-actin mouse Immunoblotting 1:1000 Sigma, A− 2228 anti-clathrin mouse Immunoblotting 1:1000 Santa Cruz, sc− 12734 anti-Myosin II human Immunofluorescence 1:100 Recombinant antibody (continued on next page) (continued ) platform, Institut Curie, Paris, France anti-CT529 rabbit Immunoblotting Immunofluorescence 1:500 1:50 Agathe Subtil, Pasteur Institute, Paris, France Alexa 488- labeled anti-mouseIgG goat Immunofluorescence 1:500 Jackson ImmunoResearch Laboratories Alexa 488- labeled antihuman-IgG goat Immunofluorescence 1:500 Jackson ImmunoResearch Laboratories HRPconjugated anti-rabbitIgG goat Immunoblotting 1:5000 Jackson ImmunoResearch Laboratories HRPconjugated anti-mouseIgG antibodies goat Immunoblotting 1:5000 Jackson ImmunoResearch Laboratories DNA was stained with DAPI (Life Technologies). .. Actin was visualized by incubation with Alexa Fluor 568 Phalloidin (Invitrogen, A12380) in PBS/1 % BSA for 1 h at 37◦C.

    Recombinant:

    Article Title: Chlamydia trachomatis highjacks host MYO1C for actin cage recruitment at the bacterial inclusion.
    Article Snippet: .. Antibodies, reagents and plasmids Antibodies Species Experiment Dilution Reference anti-MYO1C mouse Immunoblotting Immunofluorescence 1:800 1:200 Santa Cruz, sc− 136544 anti-actin mouse Immunoblotting 1:1000 Sigma, A− 2228 anti-clathrin mouse Immunoblotting 1:1000 Santa Cruz, sc− 12734 anti-Myosin II human Immunofluorescence 1:100 Recombinant antibody (continued on next page) (continued ) platform, Institut Curie, Paris, France anti-CT529 rabbit Immunoblotting Immunofluorescence 1:500 1:50 Agathe Subtil, Pasteur Institute, Paris, France Alexa 488- labeled anti-mouseIgG goat Immunofluorescence 1:500 Jackson ImmunoResearch Laboratories Alexa 488- labeled antihuman-IgG goat Immunofluorescence 1:500 Jackson ImmunoResearch Laboratories HRPconjugated anti-rabbitIgG goat Immunoblotting 1:5000 Jackson ImmunoResearch Laboratories HRPconjugated anti-mouseIgG antibodies goat Immunoblotting 1:5000 Jackson ImmunoResearch Laboratories DNA was stained with DAPI (Life Technologies). .. Actin was visualized by incubation with Alexa Fluor 568 Phalloidin (Invitrogen, A12380) in PBS/1 % BSA for 1 h at 37◦C.

    Labeling:

    Article Title: Chlamydia trachomatis highjacks host MYO1C for actin cage recruitment at the bacterial inclusion.
    Article Snippet: .. Antibodies, reagents and plasmids Antibodies Species Experiment Dilution Reference anti-MYO1C mouse Immunoblotting Immunofluorescence 1:800 1:200 Santa Cruz, sc− 136544 anti-actin mouse Immunoblotting 1:1000 Sigma, A− 2228 anti-clathrin mouse Immunoblotting 1:1000 Santa Cruz, sc− 12734 anti-Myosin II human Immunofluorescence 1:100 Recombinant antibody (continued on next page) (continued ) platform, Institut Curie, Paris, France anti-CT529 rabbit Immunoblotting Immunofluorescence 1:500 1:50 Agathe Subtil, Pasteur Institute, Paris, France Alexa 488- labeled anti-mouseIgG goat Immunofluorescence 1:500 Jackson ImmunoResearch Laboratories Alexa 488- labeled antihuman-IgG goat Immunofluorescence 1:500 Jackson ImmunoResearch Laboratories HRPconjugated anti-rabbitIgG goat Immunoblotting 1:5000 Jackson ImmunoResearch Laboratories HRPconjugated anti-mouseIgG antibodies goat Immunoblotting 1:5000 Jackson ImmunoResearch Laboratories DNA was stained with DAPI (Life Technologies). .. Actin was visualized by incubation with Alexa Fluor 568 Phalloidin (Invitrogen, A12380) in PBS/1 % BSA for 1 h at 37◦C.

    Staining:

    Article Title: Chlamydia trachomatis highjacks host MYO1C for actin cage recruitment at the bacterial inclusion.
    Article Snippet: .. Antibodies, reagents and plasmids Antibodies Species Experiment Dilution Reference anti-MYO1C mouse Immunoblotting Immunofluorescence 1:800 1:200 Santa Cruz, sc− 136544 anti-actin mouse Immunoblotting 1:1000 Sigma, A− 2228 anti-clathrin mouse Immunoblotting 1:1000 Santa Cruz, sc− 12734 anti-Myosin II human Immunofluorescence 1:100 Recombinant antibody (continued on next page) (continued ) platform, Institut Curie, Paris, France anti-CT529 rabbit Immunoblotting Immunofluorescence 1:500 1:50 Agathe Subtil, Pasteur Institute, Paris, France Alexa 488- labeled anti-mouseIgG goat Immunofluorescence 1:500 Jackson ImmunoResearch Laboratories Alexa 488- labeled antihuman-IgG goat Immunofluorescence 1:500 Jackson ImmunoResearch Laboratories HRPconjugated anti-rabbitIgG goat Immunoblotting 1:5000 Jackson ImmunoResearch Laboratories HRPconjugated anti-mouseIgG antibodies goat Immunoblotting 1:5000 Jackson ImmunoResearch Laboratories DNA was stained with DAPI (Life Technologies). .. Actin was visualized by incubation with Alexa Fluor 568 Phalloidin (Invitrogen, A12380) in PBS/1 % BSA for 1 h at 37◦C.



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