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anti ig human lambda light chain biotin  (Miltenyi Biotec)


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    Miltenyi Biotec anti ig human lambda light chain biotin
    Anti Ig Human Lambda Light Chain Biotin, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ig+lambda+light+chain/Ig+%CE%BB+Light+Chain+Antibody%2C+anti-human%2C+Biotin/pmc08977869-439-6-11
    Average 91 stars, based on 8 article reviews
    anti ig human lambda light chain biotin - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    other:

    Article Title: Vaccine protection against rectal acquisition of SIVmac239 in rhesus macaques
    Article Snippet: Next, 50 μl of Anti-Ig human lambda light chain-biotin (Miltenyi Biotec, Inc.) diluted 1:100 in dilution buffer was added to each well.

    Binding Assay:

    Article Title: Total body CD4 + T cell dynamics in treated and untreated SIV infection revealed by in vivo imaging
    Article Snippet: .. A secondary mouse anti–human λ light chain conjugated to biotin (Miltenyi Biotec) and streptavidin/HRP system was used to detect binding of monkey IgG to the immobilized antigens. ..

    Incubation:

    Article Title: Rare Control of SIVmac239 Infection in a Vaccinated Rhesus Macaque
    Article Snippet: .. Subsequently, plates were washed five times, and 100 μL of a 100-fold dilution of anti-immunoglobulin (Ig) human λ light chain conjugated to biotin (Miltenyi Biotec) was added to each well and incubated for 1 h. Plates were then washed five times before 100 μL of a 10,000-fold dilution of horseradish peroxidase-conjugated Streptavidin (Invitrogen) was added to each well. ..

    Article Title: Blocking α 4 β 7 integrin delays viral rebound in SHIV SF162P3 -infected macaques treated with anti-HIV broadly neutralizing antibodies
    Article Snippet: .. Plates were washed and incubated with anti-Ig human lambda light chain–biotin (Miltenyi Biotec), which does not recognize the kappa chain of the Rh-α 4 β 7 for 1 hour at room temperature. .. Plates were washed and incubated with diluted streptavidin-HRP (Invitrogen) for 1 hour at room temperature.

    Article Title: Protection of rhesus macaques against vaginal SHIV challenges by VRC01 and an anti-α 4 β 7 antibody
    Article Snippet: .. Plates were washed and incubated with anti-Ig human lambda light chain-biotin (Miltenyi), which does not recognize the kappa chain of the Rh-α 4 β 7 1 hour at RT. .. Plates were washed and incubated with diluted streptavidin-HRP (Invitrogen) 1 hour at RT.

    Article Title: Delayed vaginal SHIV infection in VRC01 and anti-α4β7 treated rhesus macaques
    Article Snippet: .. Plates were washed and incubated with anti-Ig human lambda light chain-biotin (Miltenyi), which does not recognize the kappa chain of the Rh-α 4 β 7 1 hour at room temperature. .. Plates were washed and incubated with diluted streptavidin-HRP (Invitrogen) 1 hour at room temperature.



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    B cells were enriched from blood of 3 healthy volunteers and infected with live or UV-inactivated purified PUUV, together with Sendai and Sindbis viruses, for comparison (multiplicity of infections for all viruses equals 1). The TLR9 agonist CpG and no treatment were used as a positive and negative controls for B cell activation, respectively. After culturing for 8 days, kappa and <t>lambda</t> FLCs in (A) and IgA, IgM and IgGs in (B) were measured from cell supernatants. Remaining cells were subjected to IgA, IgM and IgG-specific antibody secreting cell (ASC) quantification using Elispot. IgA + and IgM + ASCs were simultaneously detected in one well, using AP-conjugated anti-IgA and HRP-conjugated anti-IgM Abs, and IgG + ASCs in a separate well using HRP-conjugated anti-IgG Ab. Representative Elispot images from one donor are shown in (C), and ASC quantification per input cell number measured from all donors in (D). Significant differences of each treatment group, as compared to non-treated cells, were assessed using two-way ANOVA and Dunnett’s multiple comparison tests. *p < 0.05, **p < 0.01, ***p < 0.001.
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    Image Search Results


    Journal: iScience

    Article Title: Preclinical safety and efficacy of a therapeutic antibody that targets SARS-CoV-2 at the sotrovimab face but is escaped by Omicron

    doi: 10.1016/j.isci.2023.106323

    Figure Lengend Snippet:

    Article Snippet: Anti-Lambda-PE (clone IS7-24C) , Miltenyi Biotec , Cat# 130-119-778; RRID: AB_2751837.

    Techniques: Virus, Recombinant, Blocking Assay, Saline, Enzyme-linked Immunosorbent Assay, Membrane, Plasmid Preparation, Expressing, Software

    B cells were enriched from blood of 3 healthy volunteers and infected with live or UV-inactivated purified PUUV, together with Sendai and Sindbis viruses, for comparison (multiplicity of infections for all viruses equals 1). The TLR9 agonist CpG and no treatment were used as a positive and negative controls for B cell activation, respectively. After culturing for 8 days, kappa and lambda FLCs in (A) and IgA, IgM and IgGs in (B) were measured from cell supernatants. Remaining cells were subjected to IgA, IgM and IgG-specific antibody secreting cell (ASC) quantification using Elispot. IgA + and IgM + ASCs were simultaneously detected in one well, using AP-conjugated anti-IgA and HRP-conjugated anti-IgM Abs, and IgG + ASCs in a separate well using HRP-conjugated anti-IgG Ab. Representative Elispot images from one donor are shown in (C), and ASC quantification per input cell number measured from all donors in (D). Significant differences of each treatment group, as compared to non-treated cells, were assessed using two-way ANOVA and Dunnett’s multiple comparison tests. *p < 0.05, **p < 0.01, ***p < 0.001.

    Journal: PLoS Pathogens

    Article Title: Hantavirus infection-induced B cell activation elevates free light chains levels in circulation

    doi: 10.1371/journal.ppat.1009843

    Figure Lengend Snippet: B cells were enriched from blood of 3 healthy volunteers and infected with live or UV-inactivated purified PUUV, together with Sendai and Sindbis viruses, for comparison (multiplicity of infections for all viruses equals 1). The TLR9 agonist CpG and no treatment were used as a positive and negative controls for B cell activation, respectively. After culturing for 8 days, kappa and lambda FLCs in (A) and IgA, IgM and IgGs in (B) were measured from cell supernatants. Remaining cells were subjected to IgA, IgM and IgG-specific antibody secreting cell (ASC) quantification using Elispot. IgA + and IgM + ASCs were simultaneously detected in one well, using AP-conjugated anti-IgA and HRP-conjugated anti-IgM Abs, and IgG + ASCs in a separate well using HRP-conjugated anti-IgG Ab. Representative Elispot images from one donor are shown in (C), and ASC quantification per input cell number measured from all donors in (D). Significant differences of each treatment group, as compared to non-treated cells, were assessed using two-way ANOVA and Dunnett’s multiple comparison tests. *p < 0.05, **p < 0.01, ***p < 0.001.

    Article Snippet: The following mAbs were used: anti-kappa light chain PercP-Cy5.5 (G20-193), anti-lambda light chain BV605 (JDC-12), anti-IgM APC (G20-127), anti-IgG AF700 (G18-145; all from BD) and anti-IgA PE-Vio770 (IS11-8E10; Miltenyi).

    Techniques: Infection, Purification, Comparison, Activation Assay, Enzyme-linked Immunospot