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anti lambda light chain bv605  (Miltenyi Biotec)


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    Miltenyi Biotec anti lambda light chain bv605
    B cells were enriched from blood of 3 healthy volunteers and infected with live or UV-inactivated purified PUUV, together with Sendai and Sindbis viruses, for comparison (multiplicity of infections for all viruses equals 1). The TLR9 agonist CpG and no treatment were used as a positive and negative controls for B cell activation, respectively. After culturing for 8 days, kappa and <t>lambda</t> FLCs in (A) and IgA, IgM and IgGs in (B) were measured from cell supernatants. Remaining cells were subjected to IgA, IgM and IgG-specific antibody secreting cell (ASC) quantification using Elispot. IgA + and IgM + ASCs were simultaneously detected in one well, using AP-conjugated anti-IgA and HRP-conjugated anti-IgM Abs, and IgG + ASCs in a separate well using HRP-conjugated anti-IgG Ab. Representative Elispot images from one donor are shown in (C), and ASC quantification per input cell number measured from all donors in (D). Significant differences of each treatment group, as compared to non-treated cells, were assessed using two-way ANOVA and Dunnett’s multiple comparison tests. *p < 0.05, **p < 0.01, ***p < 0.001.
    Anti Lambda Light Chain Bv605, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ig+lambda+light+chain/Ig+%CE%BB+Light+Chain+Antibody%2C+anti-human%2C+pure/pmc08382192-95-10-28
    Average 91 stars, based on 8 article reviews
    anti lambda light chain bv605 - by Bioz Stars, 2026-11
    91/100 stars

    Images

    1) Product Images from "Hantavirus infection-induced B cell activation elevates free light chains levels in circulation"

    Article Title: Hantavirus infection-induced B cell activation elevates free light chains levels in circulation

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1009843

    B cells were enriched from blood of 3 healthy volunteers and infected with live or UV-inactivated purified PUUV, together with Sendai and Sindbis viruses, for comparison (multiplicity of infections for all viruses equals 1). The TLR9 agonist CpG and no treatment were used as a positive and negative controls for B cell activation, respectively. After culturing for 8 days, kappa and lambda FLCs in (A) and IgA, IgM and IgGs in (B) were measured from cell supernatants. Remaining cells were subjected to IgA, IgM and IgG-specific antibody secreting cell (ASC) quantification using Elispot. IgA + and IgM + ASCs were simultaneously detected in one well, using AP-conjugated anti-IgA and HRP-conjugated anti-IgM Abs, and IgG + ASCs in a separate well using HRP-conjugated anti-IgG Ab. Representative Elispot images from one donor are shown in (C), and ASC quantification per input cell number measured from all donors in (D). Significant differences of each treatment group, as compared to non-treated cells, were assessed using two-way ANOVA and Dunnett’s multiple comparison tests. *p < 0.05, **p < 0.01, ***p < 0.001.
    Figure Legend Snippet: B cells were enriched from blood of 3 healthy volunteers and infected with live or UV-inactivated purified PUUV, together with Sendai and Sindbis viruses, for comparison (multiplicity of infections for all viruses equals 1). The TLR9 agonist CpG and no treatment were used as a positive and negative controls for B cell activation, respectively. After culturing for 8 days, kappa and lambda FLCs in (A) and IgA, IgM and IgGs in (B) were measured from cell supernatants. Remaining cells were subjected to IgA, IgM and IgG-specific antibody secreting cell (ASC) quantification using Elispot. IgA + and IgM + ASCs were simultaneously detected in one well, using AP-conjugated anti-IgA and HRP-conjugated anti-IgM Abs, and IgG + ASCs in a separate well using HRP-conjugated anti-IgG Ab. Representative Elispot images from one donor are shown in (C), and ASC quantification per input cell number measured from all donors in (D). Significant differences of each treatment group, as compared to non-treated cells, were assessed using two-way ANOVA and Dunnett’s multiple comparison tests. *p < 0.05, **p < 0.01, ***p < 0.001.

    Techniques Used: Infection, Purification, Comparison, Activation Assay, Enzyme-linked Immunospot

    Related Articles

    Infection:

    Article Title: Hantavirus infection-induced B cell activation elevates free light chains levels in circulation
    Article Snippet: (eBioscience fixation and permeabilization buffer; Thermo Scientific), and stained with mAbs against intracellular antigens for 30 min at RT. .. The following mAbs were used: anti-kappa light chain PercP-Cy5.5 (G20-193), anti-lambda light chain BV605 (JDC-12), anti-IgM APC (G20-127), anti-IgG AF700 (G18-145; all from BD) and anti-IgA PE-Vio770 (IS11-8E10; Miltenyi). .. In the experiments with AF647-conjugated bank vole mAb recognizing PUUV N protein (1C12; [ , ]) the anti-IgM APC was replaced by anti-IgM V450 (G20-12

    Purification:

    Article Title: Hantavirus infection-induced B cell activation elevates free light chains levels in circulation
    Article Snippet: (eBioscience fixation and permeabilization buffer; Thermo Scientific), and stained with mAbs against intracellular antigens for 30 min at RT. .. The following mAbs were used: anti-kappa light chain PercP-Cy5.5 (G20-193), anti-lambda light chain BV605 (JDC-12), anti-IgM APC (G20-127), anti-IgG AF700 (G18-145; all from BD) and anti-IgA PE-Vio770 (IS11-8E10; Miltenyi). .. In the experiments with AF647-conjugated bank vole mAb recognizing PUUV N protein (1C12; [ , ]) the anti-IgM APC was replaced by anti-IgM V450 (G20-12

    Comparison:

    Article Title: Hantavirus infection-induced B cell activation elevates free light chains levels in circulation
    Article Snippet: (eBioscience fixation and permeabilization buffer; Thermo Scientific), and stained with mAbs against intracellular antigens for 30 min at RT. .. The following mAbs were used: anti-kappa light chain PercP-Cy5.5 (G20-193), anti-lambda light chain BV605 (JDC-12), anti-IgM APC (G20-127), anti-IgG AF700 (G18-145; all from BD) and anti-IgA PE-Vio770 (IS11-8E10; Miltenyi). .. In the experiments with AF647-conjugated bank vole mAb recognizing PUUV N protein (1C12; [ , ]) the anti-IgM APC was replaced by anti-IgM V450 (G20-12

    Activation Assay:

    Article Title: Hantavirus infection-induced B cell activation elevates free light chains levels in circulation
    Article Snippet: (eBioscience fixation and permeabilization buffer; Thermo Scientific), and stained with mAbs against intracellular antigens for 30 min at RT. .. The following mAbs were used: anti-kappa light chain PercP-Cy5.5 (G20-193), anti-lambda light chain BV605 (JDC-12), anti-IgM APC (G20-127), anti-IgG AF700 (G18-145; all from BD) and anti-IgA PE-Vio770 (IS11-8E10; Miltenyi). .. In the experiments with AF647-conjugated bank vole mAb recognizing PUUV N protein (1C12; [ , ]) the anti-IgM APC was replaced by anti-IgM V450 (G20-12

    Enzyme-linked Immunospot:

    Article Title: Hantavirus infection-induced B cell activation elevates free light chains levels in circulation
    Article Snippet: (eBioscience fixation and permeabilization buffer; Thermo Scientific), and stained with mAbs against intracellular antigens for 30 min at RT. .. The following mAbs were used: anti-kappa light chain PercP-Cy5.5 (G20-193), anti-lambda light chain BV605 (JDC-12), anti-IgM APC (G20-127), anti-IgG AF700 (G18-145; all from BD) and anti-IgA PE-Vio770 (IS11-8E10; Miltenyi). .. In the experiments with AF647-conjugated bank vole mAb recognizing PUUV N protein (1C12; [ , ]) the anti-IgM APC was replaced by anti-IgM V450 (G20-12



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    B cells were enriched from blood of 3 healthy volunteers and infected with live or UV-inactivated purified PUUV, together with Sendai and Sindbis viruses, for comparison (multiplicity of infections for all viruses equals 1). The TLR9 agonist CpG and no treatment were used as a positive and negative controls for B cell activation, respectively. After culturing for 8 days, kappa and <t>lambda</t> FLCs in (A) and IgA, IgM and IgGs in (B) were measured from cell supernatants. Remaining cells were subjected to IgA, IgM and IgG-specific antibody secreting cell (ASC) quantification using Elispot. IgA + and IgM + ASCs were simultaneously detected in one well, using AP-conjugated anti-IgA and HRP-conjugated anti-IgM Abs, and IgG + ASCs in a separate well using HRP-conjugated anti-IgG Ab. Representative Elispot images from one donor are shown in (C), and ASC quantification per input cell number measured from all donors in (D). Significant differences of each treatment group, as compared to non-treated cells, were assessed using two-way ANOVA and Dunnett’s multiple comparison tests. *p < 0.05, **p < 0.01, ***p < 0.001.
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    Image Search Results


    Journal: iScience

    Article Title: Preclinical safety and efficacy of a therapeutic antibody that targets SARS-CoV-2 at the sotrovimab face but is escaped by Omicron

    doi: 10.1016/j.isci.2023.106323

    Figure Lengend Snippet:

    Article Snippet: Anti-Lambda-PE (clone IS7-24C) , Miltenyi Biotec , Cat# 130-119-778; RRID: AB_2751837.

    Techniques: Virus, Recombinant, Blocking Assay, Saline, Enzyme-linked Immunosorbent Assay, Membrane, Plasmid Preparation, Expressing, Software

    B cells were enriched from blood of 3 healthy volunteers and infected with live or UV-inactivated purified PUUV, together with Sendai and Sindbis viruses, for comparison (multiplicity of infections for all viruses equals 1). The TLR9 agonist CpG and no treatment were used as a positive and negative controls for B cell activation, respectively. After culturing for 8 days, kappa and lambda FLCs in (A) and IgA, IgM and IgGs in (B) were measured from cell supernatants. Remaining cells were subjected to IgA, IgM and IgG-specific antibody secreting cell (ASC) quantification using Elispot. IgA + and IgM + ASCs were simultaneously detected in one well, using AP-conjugated anti-IgA and HRP-conjugated anti-IgM Abs, and IgG + ASCs in a separate well using HRP-conjugated anti-IgG Ab. Representative Elispot images from one donor are shown in (C), and ASC quantification per input cell number measured from all donors in (D). Significant differences of each treatment group, as compared to non-treated cells, were assessed using two-way ANOVA and Dunnett’s multiple comparison tests. *p < 0.05, **p < 0.01, ***p < 0.001.

    Journal: PLoS Pathogens

    Article Title: Hantavirus infection-induced B cell activation elevates free light chains levels in circulation

    doi: 10.1371/journal.ppat.1009843

    Figure Lengend Snippet: B cells were enriched from blood of 3 healthy volunteers and infected with live or UV-inactivated purified PUUV, together with Sendai and Sindbis viruses, for comparison (multiplicity of infections for all viruses equals 1). The TLR9 agonist CpG and no treatment were used as a positive and negative controls for B cell activation, respectively. After culturing for 8 days, kappa and lambda FLCs in (A) and IgA, IgM and IgGs in (B) were measured from cell supernatants. Remaining cells were subjected to IgA, IgM and IgG-specific antibody secreting cell (ASC) quantification using Elispot. IgA + and IgM + ASCs were simultaneously detected in one well, using AP-conjugated anti-IgA and HRP-conjugated anti-IgM Abs, and IgG + ASCs in a separate well using HRP-conjugated anti-IgG Ab. Representative Elispot images from one donor are shown in (C), and ASC quantification per input cell number measured from all donors in (D). Significant differences of each treatment group, as compared to non-treated cells, were assessed using two-way ANOVA and Dunnett’s multiple comparison tests. *p < 0.05, **p < 0.01, ***p < 0.001.

    Article Snippet: The following mAbs were used: anti-kappa light chain PercP-Cy5.5 (G20-193), anti-lambda light chain BV605 (JDC-12), anti-IgM APC (G20-127), anti-IgG AF700 (G18-145; all from BD) and anti-IgA PE-Vio770 (IS11-8E10; Miltenyi).

    Techniques: Infection, Purification, Comparison, Activation Assay, Enzyme-linked Immunospot