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a human genomic dna library in lambda dash ii  (Agilent technologies)


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    Structured Review

    Agilent technologies a human genomic dna library in lambda dash ii
    A Human Genomic Dna Library In Lambda Dash Ii, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+genomic+lambda+dash+library/pm09790959-39-6-12
    Average 90 stars, based on 1 article reviews
    a human genomic dna library in lambda dash ii - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Library Screening:

    Article Title: Zaba: a novel miniature transposable element present in genomes of legume plants.
    Article Snippet: A novel family of miniature transposable elements, namedZaba, was identified in pea (Pisum sativum) and subsequently also in other legume species using computer analysis of their DNA sequences.. Zaba elements are 141–190 bp long, generate 10-bp target site duplications, and their terminal inverted repeats make up most of the sequence.Zaba elements thus resemble class 3 foldback transposons.. The elements are only moderately repetitive in pea (tens to hundreds copies per haploid genome), but they are present in up to thousands of copies in the genomes of several Medicago and Vicia species.

    Clone Assay:

    Article Title: Zaba: a novel miniature transposable element present in genomes of legume plants.
    Article Snippet: A novel family of miniature transposable elements, namedZaba, was identified in pea (Pisum sativum) and subsequently also in other legume species using computer analysis of their DNA sequences.. Zaba elements are 141–190 bp long, generate 10-bp target site duplications, and their terminal inverted repeats make up most of the sequence.Zaba elements thus resemble class 3 foldback transposons.. The elements are only moderately repetitive in pea (tens to hundreds copies per haploid genome), but they are present in up to thousands of copies in the genomes of several Medicago and Vicia species.

    Article Title: Molecular characterization and gene disruption of a novel zinc-finger protein, HIT-4, expressed in rodent brain.
    Article Snippet: .. To isolate genomic clones for HIT-4, we screened a 129/SVJ mouse genomic library in lambda DASH II (Stratagene Cloning Systems, La Jolla, CA, USA) with the above PCR product as a probe. ..

    Hybridization:

    Article Title: Zaba: a novel miniature transposable element present in genomes of legume plants.
    Article Snippet: A novel family of miniature transposable elements, namedZaba, was identified in pea (Pisum sativum) and subsequently also in other legume species using computer analysis of their DNA sequences.. Zaba elements are 141–190 bp long, generate 10-bp target site duplications, and their terminal inverted repeats make up most of the sequence.Zaba elements thus resemble class 3 foldback transposons.. The elements are only moderately repetitive in pea (tens to hundreds copies per haploid genome), but they are present in up to thousands of copies in the genomes of several Medicago and Vicia species.

    Centrifugation:

    Article Title: bryA: an unusual modular polyketide synthase gene from the uncultivated bacterial symbiont of the marine bryozoan Bugula neritina.
    Article Snippet: .. Spooling was superior to centrifugation because it reduced the amount into Lambda DASH II (Stratagene, Inc.), which accommodates fragments of 9–23 kbp. ..

    Cloning:

    Article Title: Molecular characterization and gene disruption of a novel zinc-finger protein, HIT-4, expressed in rodent brain.
    Article Snippet: .. To isolate genomic clones for HIT-4, we screened a 129/SVJ mouse genomic library in lambda DASH II (Stratagene Cloning Systems, La Jolla, CA, USA) with the above PCR product as a probe. ..

    Polymerase Chain Reaction:

    Article Title: Molecular characterization and gene disruption of a novel zinc-finger protein, HIT-4, expressed in rodent brain.
    Article Snippet: .. To isolate genomic clones for HIT-4, we screened a 129/SVJ mouse genomic library in lambda DASH II (Stratagene Cloning Systems, La Jolla, CA, USA) with the above PCR product as a probe. ..

    Article Title: Actively Transcribing RNA Polymerase II Concentrates on Spliced Leader Genes in the Nucleus of Trypanosoma cruzi
    Article Snippet: .. To clone and express the recombinant protein corresponding to the CTD of T. cruzi RNA Pol II (rCTD), a lambda DASH II (Stratagene) clone containing the genomic sequence of RNA Pol II of T. cruzi ( 16 ) was used as a template for PCR with oligonucleotides CTDFor (5′ CCCATATGGGCGGCAGCTCCTCCGC) and CTDRev (5′CGGATCCCTACTGGTGCCCTTCCTC). .. The PCR product was inserted into pGEM-T-Easy (Promega, Madison, WI) and then into the NdeI-BamHI sites of pET14b (Novagen).

    other:

    Article Title: A RelA-SpoT homolog (Cr-RSH) identified in Chlamydomonas reinhardtii generates stringent factor in vivo and localizes to chloroplasts in vitro
    Article Snippet: Genomic DNA prepared from C.reinhardtii was partially digested with Sau 3AI, and the resulting fragments of ∼10– 15 kb were size-fractionated by Tris–acetate–EDTA (TAE) agarose gel (0.8%) electrophoresis and cloned into lambda-DASH II (Stratagene).

    Article Title: The AeAct-4 gene is expressed in the developing flight muscles of female Aedes aegypti.
    Article Snippet: An Ae. aegypti genomic library in Lambda DASH® II (Stratagene, La Jolla, CA), was screened initially using a PCR-based method as described (Israel, 1993).

    Recombinant:

    Article Title: Actively Transcribing RNA Polymerase II Concentrates on Spliced Leader Genes in the Nucleus of Trypanosoma cruzi
    Article Snippet: .. To clone and express the recombinant protein corresponding to the CTD of T. cruzi RNA Pol II (rCTD), a lambda DASH II (Stratagene) clone containing the genomic sequence of RNA Pol II of T. cruzi ( 16 ) was used as a template for PCR with oligonucleotides CTDFor (5′ CCCATATGGGCGGCAGCTCCTCCGC) and CTDRev (5′CGGATCCCTACTGGTGCCCTTCCTC). .. The PCR product was inserted into pGEM-T-Easy (Promega, Madison, WI) and then into the NdeI-BamHI sites of pET14b (Novagen).

    Sequencing:

    Article Title: Actively Transcribing RNA Polymerase II Concentrates on Spliced Leader Genes in the Nucleus of Trypanosoma cruzi
    Article Snippet: .. To clone and express the recombinant protein corresponding to the CTD of T. cruzi RNA Pol II (rCTD), a lambda DASH II (Stratagene) clone containing the genomic sequence of RNA Pol II of T. cruzi ( 16 ) was used as a template for PCR with oligonucleotides CTDFor (5′ CCCATATGGGCGGCAGCTCCTCCGC) and CTDRev (5′CGGATCCCTACTGGTGCCCTTCCTC). .. The PCR product was inserted into pGEM-T-Easy (Promega, Madison, WI) and then into the NdeI-BamHI sites of pET14b (Novagen).



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