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human plasma  (Assaypro)


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    Structured Review

    Assaypro human plasma
    Human Plasma, supplied by Assaypro, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+complement+plasma/Human+Complement+Factor+B+Deficient+Plasma/pmc06742440-108-7-17
    Average 90 stars, based on 1 article reviews
    human plasma - by Bioz Stars, 2026-11
    90/100 stars

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    Clinical Proteomics:

    Article Title: Packaging functionally-important plasma proteins into the α-granules of human induced pluripotent stem cell-derived megakaryocytes
    Article Snippet: The HPCs were further differentiated to MKs in serum-free differentiation (SFD) medium: Iscove’s Modified Dulbecco’s Medium (IMDM) (Thermo Fisher Scientific) containing 25% Ham’s F12 (Corning), 0.5% N2 (Thermo Fisher Scientific), 1% B27 without Vitamin A (Thermo Fisher Scientific), 0.05% BSA (Sigma), 2mM L-glutamine and penicillin/streptomycin supplemented with 50 ng/ml SCF (R&D systems) and 50 ng/ml TPO (R&D systems) at 37 °C, 5% CO 2 for 7 days. .. To load MKs with proteins present in human plasma, 10% heparinized type AB human complement C5-deficient plasma (Assaypro) was added to culture medium on day 6. ..

    Article Title: Role of inflammation in sensory neuropathy in prediabetes or diabetes.
    Article Snippet: Objectives – Prediabetes includes individuals with impaired glucose metabolism, and it has been associated with various complications of diabetes mellitus (DM), including peripheral neuropathy.. We aimed to investigate the associations between pro-inflammatory (TNF-a) and anti-inflammatory (IL-10) cytokines and neuropathy of very distal sensory nerves in patients with prediabetes or type 2 DM.. Materials and Methods – We included 50 patients with prediabetes, 50 patients with type 2 DM, and 44 controls in the study.

    Article Title: High sCD40L levels early after trauma are associated with enhanced shock, sympathoadrenal activation, tissue and endothelial damage, coagulopathy and mortality.
    Article Snippet: Citrate plasma: protein C (PC, Helena Laboratories, Beaumont, TX, USA; LLD 5% of reference plasma); activated protein C (APC, USCNLIFE; LLD 4.2 pg mL)1); soluble endothelial protein C receptor (sEPCR; R&D Systems Europe; LLD 0.064 ng mL)1); protein S (PS, ADI; LLD not stated, quantified relative to provided reference plasma); tissue-type plasminogen activator (tPA, ADI, detects sc-tPA, tc-tPA and tPA/PAI-1 complexes; LLD 1 ng mL)1); plasminogen activator inhibitor-1 (PAI-1, Assaypro, Sct. .. Charles, MO, USA; LLD 0.2 ng mL)1); prothrombinfragment 1 and 2 (PF1.2, USCNLIFE; LLD 0.043 nmol L)1); (TAT, USCNLIFE; LLD 0.215 ng mL)1); tissue factor pathway inhibitor (TFPI, ADI, detects intact TFPI, truncated TFPI, TF/FVIIa/TFPI complexes; LLD 0.18 ng mL)1); von Willebrand factor antigen (VWF, Helena Laboratories, LLD 5% of reference plasma); factor (F) XIII (Assaypro; LLD 50 pg mL)1); terminal complement complex (sC5b-9, MicroVue sC5b-9 plus EIA Kit; Quidel Corp., San Diego, CA, USA; LLD 3.7 ng mL)1); and interleukin-6 (IL-6, Quantikine HS; R&D Systems Europe; LLD 0.039 pg mL)1). .. Serum: Syndecan-1 (Diaclone SAS, Besancon, France; LLD 2.56 ng mL)1).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Role of inflammation in sensory neuropathy in prediabetes or diabetes.
    Article Snippet: Objectives – Prediabetes includes individuals with impaired glucose metabolism, and it has been associated with various complications of diabetes mellitus (DM), including peripheral neuropathy.. We aimed to investigate the associations between pro-inflammatory (TNF-a) and anti-inflammatory (IL-10) cytokines and neuropathy of very distal sensory nerves in patients with prediabetes or type 2 DM.. Materials and Methods – We included 50 patients with prediabetes, 50 patients with type 2 DM, and 44 controls in the study.

    Article Title: High sCD40L levels early after trauma are associated with enhanced shock, sympathoadrenal activation, tissue and endothelial damage, coagulopathy and mortality.
    Article Snippet: Citrate plasma: protein C (PC, Helena Laboratories, Beaumont, TX, USA; LLD 5% of reference plasma); activated protein C (APC, USCNLIFE; LLD 4.2 pg mL)1); soluble endothelial protein C receptor (sEPCR; R&D Systems Europe; LLD 0.064 ng mL)1); protein S (PS, ADI; LLD not stated, quantified relative to provided reference plasma); tissue-type plasminogen activator (tPA, ADI, detects sc-tPA, tc-tPA and tPA/PAI-1 complexes; LLD 1 ng mL)1); plasminogen activator inhibitor-1 (PAI-1, Assaypro, Sct. .. Charles, MO, USA; LLD 0.2 ng mL)1); prothrombinfragment 1 and 2 (PF1.2, USCNLIFE; LLD 0.043 nmol L)1); (TAT, USCNLIFE; LLD 0.215 ng mL)1); tissue factor pathway inhibitor (TFPI, ADI, detects intact TFPI, truncated TFPI, TF/FVIIa/TFPI complexes; LLD 0.18 ng mL)1); von Willebrand factor antigen (VWF, Helena Laboratories, LLD 5% of reference plasma); factor (F) XIII (Assaypro; LLD 50 pg mL)1); terminal complement complex (sC5b-9, MicroVue sC5b-9 plus EIA Kit; Quidel Corp., San Diego, CA, USA; LLD 3.7 ng mL)1); and interleukin-6 (IL-6, Quantikine HS; R&D Systems Europe; LLD 0.039 pg mL)1). .. Serum: Syndecan-1 (Diaclone SAS, Besancon, France; LLD 2.56 ng mL)1).



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    Anti-KLRG1 monoclonal antibodies exert no apoptosis but induce ADCP, ADCC, and <t>complement-mediated</t> cytotoxicity (CDC). A, SMZ1-WT and SMZ1-KLRG1 cells were incubated with the indicated antibodies (mAb208, 13F12F2, or its isotype control mIgG2aκ) at increasing concentrations from 1 to 100 μg/mL or 0.2 or 1 μmol/L staurosporine (positive control) for 24 hours, and the percentage of apoptotic cells was quantified by flow cytometry. Three independent experiments were conducted. Shown are the mean values of technical triplicates with error bars representing SEM from one representative experiment. B–C, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human monocyte-derived macrophages (hMDM) from three healthy donors and bone-marrow–derived murine macrophages (mBMDM) from different lots of mice in the presence of indicated antibodies for 2 hours. Anti-CD47 mAb, MIAP410-mediated phagocytosis of Jurkat cells relative to its isotype control, mIgG1κ, was used as a positive control. The percentage of CFSE + macrophages (of human CD14 + or murine CD11b + cells) was determined by flow cytometry and plotted. D–E, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human-derived PBMCs from three healthy donors in the presence of indicated antibodies at increasing concentrations for 4 hours. The percentage of propidium iodide–positive (% PI pos; of CFSE + ) cells was determined by flow cytometry and plotted. Rituximab and Raji cells were used as a positive control. F–G, ADCC reporter assay measuring luminescence with murine FcγRIIIA-expressing Jurkat cells was performed in triplicate in three independent experiments at an effector:target ratio of 25:1 with indicated antibodies. Fold induction is relative to isotype control (mIgG2aκ for mAb208 and 13F12F2). Human anti-CD20 mAb and Raji cells were used as positive control along with its isotype control, hIgG1. Representative results from one experiment are shown. H–K, CDC assay with 30% murine and human complement was performed in triplicate in three independent experiments, and % PI pos cells were reported. Rituximab and Raji cells were used as positive controls. Shown are the mean values of technical triplicates, with error bars representing SEM from one representative experiment. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by two-sided Welch t test.
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    Image Search Results


    Anti-KLRG1 monoclonal antibodies exert no apoptosis but induce ADCP, ADCC, and complement-mediated cytotoxicity (CDC). A, SMZ1-WT and SMZ1-KLRG1 cells were incubated with the indicated antibodies (mAb208, 13F12F2, or its isotype control mIgG2aκ) at increasing concentrations from 1 to 100 μg/mL or 0.2 or 1 μmol/L staurosporine (positive control) for 24 hours, and the percentage of apoptotic cells was quantified by flow cytometry. Three independent experiments were conducted. Shown are the mean values of technical triplicates with error bars representing SEM from one representative experiment. B–C, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human monocyte-derived macrophages (hMDM) from three healthy donors and bone-marrow–derived murine macrophages (mBMDM) from different lots of mice in the presence of indicated antibodies for 2 hours. Anti-CD47 mAb, MIAP410-mediated phagocytosis of Jurkat cells relative to its isotype control, mIgG1κ, was used as a positive control. The percentage of CFSE + macrophages (of human CD14 + or murine CD11b + cells) was determined by flow cytometry and plotted. D–E, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human-derived PBMCs from three healthy donors in the presence of indicated antibodies at increasing concentrations for 4 hours. The percentage of propidium iodide–positive (% PI pos; of CFSE + ) cells was determined by flow cytometry and plotted. Rituximab and Raji cells were used as a positive control. F–G, ADCC reporter assay measuring luminescence with murine FcγRIIIA-expressing Jurkat cells was performed in triplicate in three independent experiments at an effector:target ratio of 25:1 with indicated antibodies. Fold induction is relative to isotype control (mIgG2aκ for mAb208 and 13F12F2). Human anti-CD20 mAb and Raji cells were used as positive control along with its isotype control, hIgG1. Representative results from one experiment are shown. H–K, CDC assay with 30% murine and human complement was performed in triplicate in three independent experiments, and % PI pos cells were reported. Rituximab and Raji cells were used as positive controls. Shown are the mean values of technical triplicates, with error bars representing SEM from one representative experiment. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by two-sided Welch t test.

    Journal: Clinical Cancer Research

    Article Title: KLRG1 Cell Depletion as a Novel Therapeutic Strategy in Patients with Mature T-Cell Lymphoma Subtypes

    doi: 10.1158/1078-0432.CCR-23-3504

    Figure Lengend Snippet: Anti-KLRG1 monoclonal antibodies exert no apoptosis but induce ADCP, ADCC, and complement-mediated cytotoxicity (CDC). A, SMZ1-WT and SMZ1-KLRG1 cells were incubated with the indicated antibodies (mAb208, 13F12F2, or its isotype control mIgG2aκ) at increasing concentrations from 1 to 100 μg/mL or 0.2 or 1 μmol/L staurosporine (positive control) for 24 hours, and the percentage of apoptotic cells was quantified by flow cytometry. Three independent experiments were conducted. Shown are the mean values of technical triplicates with error bars representing SEM from one representative experiment. B–C, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human monocyte-derived macrophages (hMDM) from three healthy donors and bone-marrow–derived murine macrophages (mBMDM) from different lots of mice in the presence of indicated antibodies for 2 hours. Anti-CD47 mAb, MIAP410-mediated phagocytosis of Jurkat cells relative to its isotype control, mIgG1κ, was used as a positive control. The percentage of CFSE + macrophages (of human CD14 + or murine CD11b + cells) was determined by flow cytometry and plotted. D–E, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human-derived PBMCs from three healthy donors in the presence of indicated antibodies at increasing concentrations for 4 hours. The percentage of propidium iodide–positive (% PI pos; of CFSE + ) cells was determined by flow cytometry and plotted. Rituximab and Raji cells were used as a positive control. F–G, ADCC reporter assay measuring luminescence with murine FcγRIIIA-expressing Jurkat cells was performed in triplicate in three independent experiments at an effector:target ratio of 25:1 with indicated antibodies. Fold induction is relative to isotype control (mIgG2aκ for mAb208 and 13F12F2). Human anti-CD20 mAb and Raji cells were used as positive control along with its isotype control, hIgG1. Representative results from one experiment are shown. H–K, CDC assay with 30% murine and human complement was performed in triplicate in three independent experiments, and % PI pos cells were reported. Rituximab and Raji cells were used as positive controls. Shown are the mean values of technical triplicates, with error bars representing SEM from one representative experiment. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by two-sided Welch t test.

    Article Snippet: Human male AB plasma as a source of human complement was obtained from Sigma-Aldrich.

    Techniques: Flow Cytometry Complement-Mediated Cytotoxicity Assay, Incubation, Positive Control, Flow Cytometry, Labeling, Derivative Assay, Reporter Assay, Expressing, CDC Assay