circrna scarb1 (Addgene inc)
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circrna scarb1
Circrna Scarb1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+circrna+array+analysis/pcDNA3%2E1(%2B)+CircRNA+Mini+Vector-ZKSCAN1+nt+548-1047+(Plasmid+%2360649)/pmc08292958-69-2-8
Average 93 stars, based on 23 article reviews
Circrna Scarb1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+circrna+array+analysis/pcDNA3%2E1(%2B)+CircRNA+Mini+Vector-ZKSCAN1+nt+548-1047+(Plasmid+%2360649)/pmc08292958-69-2-8
Average 93 stars, based on 23 article reviews
circrna scarb1 - by Bioz Stars,
2026-10
93/100 stars
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1) Product Images from "The Maturation of Tumor Suppressor miR-497 in Hepatocellular Carcinoma is Inhibited by Oncogenic circRNA SCARB1"
Article Title: The Maturation of Tumor Suppressor miR-497 in Hepatocellular Carcinoma is Inhibited by Oncogenic circRNA SCARB1
Journal: Cancer Management and Research
doi: 10.2147/CMAR.S304125
Figure Legend Snippet: The expression of circRNA SCARB1, mature miR-497 and miR-497 precursor was altered in HCC. HCC and paired non-tumor tissues were collected from the 64 HCC patients included in this study, and the expression of circRNA SCARB1 ( A ), mature miR-497 ( B ) and miR-497 precursor ( C ) in these tissue samples was determined by RT-qPCR. Average values of three technical replicates were used to express gene expression data in paired HCC and non-tumor tissues, ** p < 0.01.
Techniques Used: Expressing, Quantitative RT-PCR, Gene Expression
Figure Legend Snippet: The Correlations Between Clinicopathological Variables and the Expression of circRNA SCARB1 in HCC
Techniques Used: Expressing, Infection
Figure Legend Snippet: CircRNA SCARB1 and mature miR-497 were inversely correlated across HCC samples. Pearson’s correlation coefficient analysis was used to analyze the correlations between circRNA SCARB1 and mature miR-497 ( A ) or miR-497 precursor ( B ) across HCC tissue samples.
Techniques Used:
Figure Legend Snippet: Overexpression of circRNA SCARB1 downregulated mature miR-497 in HCC cells. To explore the effects of overexpression of circRNA SCARB1 on the maturation of miR-497, SNU-423 and SNU-387 cells were transfected with either circRNA SCARB1 expression vector or miR-497 mimic, followed by the confirmation of transfections at 48 h post-transfection by RT-qPCR ( A ). The effects of overexpression of circRNA SCARB1 of the expression of mature miR-497 ( B ) and miR-497 precursor ( C ), as well as the effects of the transfection of miR-497 mimic on circRNA SCARB1 expression ( D ) were also analyzed by RT-qPCR. Mean ± SD values were used to express data of 3 biological replicates of in vitro cell experiments. * p < 0.05.
Techniques Used: Over Expression, Transfection, Expressing, Plasmid Preparation, Quantitative RT-PCR, In Vitro
Figure Legend Snippet: Overexpression of circRNA SCARB1 increased HCC cell proliferation through miR-497. CCK-8 assay was performed to analyze the effects of overexpression of circRNA SCARB1 and miR-497 on the proliferation of SNU-423 ( A ) and SNU-387 cells ( B ). Mean ± SD values were used to express data of three biological replicates of in vitro cell experiments. * p < 0.05.
Techniques Used: Over Expression, CCK-8 Assay, In Vitro
Figure Legend Snippet: Overexpression of circRNA SCARB1 increased HCC cell migration through miR-497. Transwell assay was conducted to analyze the effects of overexpression of circRNA SCARB1 and miR-497 on the migration of SNU-423 ( A and C ) and SNU-387 cells ( B and D ). Mean ± SD values were used to express data of three biological replicates of in vitro cell experiments. * p < 0.05.
Techniques Used: Over Expression, Migration, Transwell Assay, In Vitro
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The OGT-570aa-3Flag sequence (Table ) was inserted into Clone Assay:Article Title: A tripartite circRNA/mRNA/miRNA interaction regulates glutamatergic signaling in the mouse brain. Article Snippet: All the mutant constructs were obtained by inverse PCR followed by phosphorylation with T4 Polynucleotide Kinase (New England Biolabs) and self-ligation of the PCR product with T4 DNA ligase (New England Biolabs). .. For the circDlc1(2) overexpression the circDlc1(2) sequence was cloned in the circRNA mini vector ZKSCAN1,43 addgene #60649, via the Article Title: A virus-induced circular RNA maintains latent infection of Kaposi sarcoma herpesvirus Article Snippet: 2 μg pcDNA3.1- hsa_circ_0001400 of plasmids was transfected to 2×10 6 cells with 12 μl Transporter 5 (Polysciences) lipofection reagent and 1 ml Opti-MemI in 10 ml DMEM. .. 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Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse C57BL/6J WT This paper N/A Mouse C57BL/6J, circDlc1(2) / This paper N/A Mouse C57BL/6J, Dlc1-206 / This paper N/A Mouse C57BL/6J WT mESCs This paper N/A Mouse C57BL/6J, circDlc1(2) / mESCs This paper N/A Mouse C57BL/6J, Dlc1-206 / mESCs This paper N/A Mouse C57BL/6J, circDlc1(2) / Rescue mESCs This paper N/A Oligonucleotides Oligonucleotides used for the genotyping in this work are listed in Table S2 This paper N/A Oligonucleotides for qPCR experiments in this work are listed in Table S2 This paper N/A Oligonucleotides for miRNA qPCR experiments in this work are listed in Table S3 Qiagen cat# 339306 Oligonucleotides for plasmid construction are listed in Table S2 This paper N/A siRNAs used in this work are listed in Table S2 This paper N/A DNA oligonucleotide probes are listed in Table S2 This paper N/A miRCURY LNA miRNA Detection Probes mmu-miR-130b-5p mmu-miR-132-3p EXIQON cat# YD00615959-BCG YD00615938-BCG Mm circ-Dlc1-specific probe for BaseScopeTM ACD cat# 708481 Recombinant DNA circDlc1(2) overexpression vector This paper N/A circDlc1(2) D130 overexpression vector This paper N/A psiCHECKTM-2 Vector Promega cat# C8021 ZKSCAN1 Kramer et al., Negative Control:Article Title: CircRNA circ_0006892 regulates miR-24/PHLPP2 axis to mitigate cigarette smoke extract-induced bronchial epithelial cell injury. 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