human circrna array v2 (4 × 180k) (CapitalBio Corporation)

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CapitalBio Corporation human circrna array v2 (4 × 180k)

The experimental scheme of the study. The discovery cohort was composed of two TB patients and two age- and gender-matched healthy controls: one young male case-control pair and one senior female case-control pair. PBMC <t>circRNA</t> expression in the discovery cohort was profiled by both rRNA-depleted RNA-seq and a circRNA expression microarray. We aggregated circRNAs into pathway-level mechanisms using KEGG pathway definitions. Pathways with significant circRNA dysregulation were prioritized by performing a paired Wilcoxon signed-rank test between paired control and TB samples. A molecular signature was developed based on the dysregulated circRNAs within the prioritized pathways. This circRNA signature was further validated by qRT-PCR in a validation cohort with 11 healthy controls and 10 TB patients.

Human Circrna Array V2 (4 × 180k), supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+circrna+array+analysis/human+lncrna+array+v4/pmc05828303-128-3-1
Average 90 stars, based on 1 article reviews

human circrna array v2 (4 × 180k) - by Bioz Stars, 2026-11

90/100 stars

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1) Product Images from "Potential Diagnostic Power of Blood Circular RNA Expression in Active Pulmonary Tuberculosis"

Article Title: Potential Diagnostic Power of Blood Circular RNA Expression in Active Pulmonary Tuberculosis

Journal: EBioMedicine

doi: 10.1016/j.ebiom.2017.12.007

Figure Legend Snippet: The experimental scheme of the study. The discovery cohort was composed of two TB patients and two age- and gender-matched healthy controls: one young male case-control pair and one senior female case-control pair. PBMC circRNA expression in the discovery cohort was profiled by both rRNA-depleted RNA-seq and a circRNA expression microarray. We aggregated circRNAs into pathway-level mechanisms using KEGG pathway definitions. Pathways with significant circRNA dysregulation were prioritized by performing a paired Wilcoxon signed-rank test between paired control and TB samples. A molecular signature was developed based on the dysregulated circRNAs within the prioritized pathways. This circRNA signature was further validated by qRT-PCR in a validation cohort with 11 healthy controls and 10 TB patients.

Techniques Used: Control, Expressing, RNA Sequencing, Microarray, Quantitative RT-PCR, Biomarker Discovery

$Landscape of PBMC circRNA expression. (a) A Venn diagram of the parental genes with circRNA transcripts among PBMCs, platelets, RBCs, and whole blood. (b) Distribution of log 2 -transformed TPM values of circular transcripts in PBMCs, platelets, RBCs, and whole blood. (c) The fraction of RNA-seq reads from coding linear, non-coding linear, and circular transcripts. (d) The cumulative distribution of TPM values of circular transcripts. A significantly increased circRNA TPM was observed in the TB patients compared with the healthy controls. The P -values were computed by the Kolmogorov-Smirnov test.$
Figure Legend Snippet: Landscape of PBMC circRNA expression. (a) A Venn diagram of the parental genes with circRNA transcripts among PBMCs, platelets, RBCs, and whole blood. (b) Distribution of log 2 -transformed TPM values of circular transcripts in PBMCs, platelets, RBCs, and whole blood. (c) The fraction of RNA-seq reads from coding linear, non-coding linear, and circular transcripts. (d) The cumulative distribution of TPM values of circular transcripts. A significantly increased circRNA TPM was observed in the TB patients compared with the healthy controls. The P -values were computed by the Kolmogorov-Smirnov test.

Techniques Used: Expressing, Transformation Assay, RNA Sequencing

$The KEGG pathways enriched by upregulated circRNAs. A paired Wilcoxon test was applied to identify the KEGG pathways enriched by dysregulated circRNAs in young and senior TB patients. (a) The correlation in Z -scores (computed by a paired Wilcoxon test) between young and senior case-control pairs. Each dot represents one KEGG pathway. The red points stand for the pathways with circular transcripts commonly upregulated in both young and senior TB patients. (b) Paired comparison of circRNA expression in the five prioritized KEGG pathways. (c) Comparison of the fraction of circRNA reads in the five pathways between healthy controls and TB patients.$
Figure Legend Snippet: The KEGG pathways enriched by upregulated circRNAs. A paired Wilcoxon test was applied to identify the KEGG pathways enriched by dysregulated circRNAs in young and senior TB patients. (a) The correlation in Z -scores (computed by a paired Wilcoxon test) between young and senior case-control pairs. Each dot represents one KEGG pathway. The red points stand for the pathways with circular transcripts commonly upregulated in both young and senior TB patients. (b) Paired comparison of circRNA expression in the five prioritized KEGG pathways. (c) Comparison of the fraction of circRNA reads in the five pathways between healthy controls and TB patients.

Techniques Used: Control, Comparison, Expressing

Technical validation of the five prioritized KEGG pathways using a circRNA expression microarray. Total RNA was treated with RNase R to remove linear transcripts and then subject to microarray hybridization.

Figure Legend Snippet: Technical validation of the five prioritized KEGG pathways using a circRNA expression microarray. Total RNA was treated with RNase R to remove linear transcripts and then subject to microarray hybridization.

Techniques Used: Biomarker Discovery, Expressing, Microarray, Hybridization

Figure Legend Snippet: The 7-circRNA signature.

Techniques Used:

The 7-circRNA signature. (a) Expression heatmap of the circRNAs within the 7-circRNA signature in the discovery cohort. The expression measured by both RNA-seq and circRNA microarray is displayed. (b) Comparison of the expression levels of the 7-circRNA signature between the healthy controls and TB patients in the validation cohort. The error bars represent the standard error of the mean. (c) Principal component analysis of the 7-circRNA signature. PC1: the first principal component; PC2: the second principal component. (d) Comparison of the circRNA-based TB index between the healthy controls and TB patients in the validation cohort. (e) The ROC curve of the TB index in distinguishing TB patients from healthy controls. The light blue area indicates the confidence interval of the ROC curve.

Figure Legend Snippet: The 7-circRNA signature. (a) Expression heatmap of the circRNAs within the 7-circRNA signature in the discovery cohort. The expression measured by both RNA-seq and circRNA microarray is displayed. (b) Comparison of the expression levels of the 7-circRNA signature between the healthy controls and TB patients in the validation cohort. The error bars represent the standard error of the mean. (c) Principal component analysis of the 7-circRNA signature. PC1: the first principal component; PC2: the second principal component. (d) Comparison of the circRNA-based TB index between the healthy controls and TB patients in the validation cohort. (e) The ROC curve of the TB index in distinguishing TB patients from healthy controls. The light blue area indicates the confidence interval of the ROC curve.

Techniques Used: Expressing, RNA Sequencing, Microarray, Comparison, Biomarker Discovery

Gene Expression:

Article Title: Bioinformatics analysis of lncRNA-related ceRNA networks in the peripheral blood lymphocytes of Kazakh patients with essential hypertension in Xinjiang
Article Snippet: Total RNA was subsequently purified using RNeasy Micro Kit (Thermo Fisher Scientific, Waltham, MA, USA) and RNase-Free DNase Set (Thermo Fisher Scientific, Waltham, MA, USA). .. In this study, CapitalBio Technology Human LncRNA Array v4 chip was used to detect the gene expression profile of human species, and the lncRNA and mRNA expression profiles in peripheral blood lymphocytes were determined by Beijing Boao Jingdian Biotechnology Co., Ltd. .. Here, 12 microarrays were prepared using 12 specimens, and the samples were labeled with the Jingxin® Biochip Universal Labeling Kit.

Article Title: Identification of a lncRNA AC011511.5- Mediated Competitive Endogenous RNA Network Involved in the Pathogenesis of Allergic Rhinitis
Article Snippet: The RNA was reverse transcribed into complementary deoxyribose nucleic acid (cDNA) by using a Total-Transcriptome cDNA Synthesis Kit (Applied Biological Materials Inc., Canada). .. CapitalBio Technology Human LncRNA Array V4, 4 × 180 K chip which was performed by Beijing Capitalbio Technology Co., Ltd., China was used to detect Human gene expression profile and the test method was numbered as AG-GE-WL10-01-20 ( ). ..

Expressing:

other:

Article Title: Elevated BICD2 DNA methylation in blood of major depressive disorder patients and reduction of depressive-like behaviors in hippocampal Bicd2 -knockdown mice
Article Snippet: The cRNA was fluorescently labeled and hybridized using a 4 × 180 K IncRNA+mRNA Human Gene Expression Microarray V3.0 (CapitalBio, Technology) to detect genome-wide gene expression.

Article Title: LncRP11-675F6.3 responds to rapamycin treatment and reduces triglyceride accumulation via interacting with HK1 in hepatocytes by regulating autophagy and VLDL-related proteins.
Article Snippet: LncRNA profiling was analyzed using LncRNA +mRNA Human Gene Expression Microarray V4.0, 4x180K (Capitalbio, Beijing, China).

Article Title: LncRP11-675F6.3 responds to rapamycin treatment and reduces triglyceride accumulation via interacting with HK1 in hepatocytes by regulating autophagy and VLDL-related proteins
Article Snippet: LncRNA profiling was analyzed using LncRNA+mRNA Human Gene Expression Microarray V4.0, 4x180K (Capitalbio, Beijing, China).

Article Title: Genome-wide identification of RNA modification-related single nucleotide polymorphisms associated with rheumatoid arthritis
Article Snippet: Genome-wide RNA expression was profiled using lncRNA&mRNA Human Gene Expression Microarray V4.0 (CapitalBio Corp, Beijing, China) according to the manufacturer’s instructions.

Article Title: SNHG 12 and hsa-miR-140-5P may play an important role in the ceRNA network related to hypertrophic cardiomyopathy
Article Snippet: LncRNA expression profiling related to HCM was conducted for 7 HCM patients and 5 disease-free individuals who were included in GSE68316 (platform: GPL20113 Capital Bio Human LncRNA Microarray v2.0; CapitalBio, Beijing, China) and for 28 HCM patients and 9 healthy donors who were included in GSE130036 (platform: GPL20795, HiSeq X Ten; Illumina, San Diego, CA, USA).

Article Title: Comprehensive analysis of lncRNA-mRNAs co-expression network identifies potential lncRNA biomarkers in cutaneous squamous cell carcinoma.
Article Snippet: The lncRNA Human Gene Expression Microarray V4.0 (CapitalBio Corp, Beijing, China) was applied for this detection.

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