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pcmv ha vector  (Addgene inc)


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    Structured Review

    Addgene inc pcmv ha vector
    Pcmv Ha Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 249 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+circrna+array+analysis/pcDNA3%2E1(%2B)+CircRNA+Mini+Vector+(Plasmid+%2360648)/pmc04488726-26-13-29
    Average 96 stars, based on 249 article reviews
    pcmv ha vector - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Over Expression:

    Article Title: Tumor cell-intrinsic circular RNA circFNDC3B attenuates CD8 + T cells infiltration in non-small cell lung cancer
    Article Snippet: Fluorescence images were captured using a confocal laser scanner, and the data were analyzed using ImageJ software. .. To establish circFNDC3B, circFndc3b, TFII-I, and STAT1 overexpression plasmids, circFNDC3B, circFndc3b, TFII-I, and STAT1 cDNA were synthesized by TSINGKE (Wuhan, China) and then cloned into pcDNA3.1(+) CircRNA Mini Vector (Addgene, Watertown, USA) and p3×FLAG-CMV-10 vector (Sigma-Aldrich, St. Louis, USA), respectively. ..

    Article Title: Circular RNA hsa_circ_0099188 Regulates Inducible Nitric Oxide Synthase and Chemokine Transcription in Macrophages by Targeting the hsa-miR-381-3p/PPP3CA and hsa-miR-381-3p/KLF4 Pathways in Response to 4,4’-Methylene Diphenyl Diisocyanate-Glutathione Conjugate Exposure
    Article Snippet: .. The circRNA overexpression vector, pcDNA3.1 (+) _CircRNA_Mini Vector, was obtained from Addgene (plasmid id#60648; Watertown, MA), deposited by Dr. Jeremy Wilusz at Baylor College of Medicine ( Liang and Wilusz 2014 ). ..

    Article Title: Tumor cell-intrinsic circular RNA circFNDC3B attenuates CD8 + T cells infiltration in non-small cell lung cancer.
    Article Snippet: Fluorescence images were captured using a confocal laser scanner, and the data were analyzed using ImageJ software. .. To establish circFNDC3B, circFndc3b, TFII-I, and STAT1 overexpression plasmids, circFNDC3B, circFndc3b, TFII-I, and STAT1 cDNA were synthesized by TSINGKE (Wuhan, China) and then cloned into pcDNA3.1(+) CircRNAMini Vector (Addgene, Watertown, USA) and p3×FLAG-CMV10 vector (Sigma-Aldrich, St. Louis, USA), respectively. ..

    Synthesized:

    Article Title: Tumor cell-intrinsic circular RNA circFNDC3B attenuates CD8 + T cells infiltration in non-small cell lung cancer
    Article Snippet: Fluorescence images were captured using a confocal laser scanner, and the data were analyzed using ImageJ software. .. To establish circFNDC3B, circFndc3b, TFII-I, and STAT1 overexpression plasmids, circFNDC3B, circFndc3b, TFII-I, and STAT1 cDNA were synthesized by TSINGKE (Wuhan, China) and then cloned into pcDNA3.1(+) CircRNA Mini Vector (Addgene, Watertown, USA) and p3×FLAG-CMV-10 vector (Sigma-Aldrich, St. Louis, USA), respectively. ..

    Article Title: Tumor cell-intrinsic circular RNA circFNDC3B attenuates CD8 + T cells infiltration in non-small cell lung cancer.
    Article Snippet: Fluorescence images were captured using a confocal laser scanner, and the data were analyzed using ImageJ software. .. To establish circFNDC3B, circFndc3b, TFII-I, and STAT1 overexpression plasmids, circFNDC3B, circFndc3b, TFII-I, and STAT1 cDNA were synthesized by TSINGKE (Wuhan, China) and then cloned into pcDNA3.1(+) CircRNAMini Vector (Addgene, Watertown, USA) and p3×FLAG-CMV10 vector (Sigma-Aldrich, St. Louis, USA), respectively. ..

    Clone Assay:

    Article Title: Tumor cell-intrinsic circular RNA circFNDC3B attenuates CD8 + T cells infiltration in non-small cell lung cancer
    Article Snippet: Fluorescence images were captured using a confocal laser scanner, and the data were analyzed using ImageJ software. .. To establish circFNDC3B, circFndc3b, TFII-I, and STAT1 overexpression plasmids, circFNDC3B, circFndc3b, TFII-I, and STAT1 cDNA were synthesized by TSINGKE (Wuhan, China) and then cloned into pcDNA3.1(+) CircRNA Mini Vector (Addgene, Watertown, USA) and p3×FLAG-CMV-10 vector (Sigma-Aldrich, St. Louis, USA), respectively. ..

    Article Title: Tumor cell-intrinsic circular RNA circFNDC3B attenuates CD8 + T cells infiltration in non-small cell lung cancer.
    Article Snippet: Fluorescence images were captured using a confocal laser scanner, and the data were analyzed using ImageJ software. .. To establish circFNDC3B, circFndc3b, TFII-I, and STAT1 overexpression plasmids, circFNDC3B, circFndc3b, TFII-I, and STAT1 cDNA were synthesized by TSINGKE (Wuhan, China) and then cloned into pcDNA3.1(+) CircRNAMini Vector (Addgene, Watertown, USA) and p3×FLAG-CMV10 vector (Sigma-Aldrich, St. Louis, USA), respectively. ..

    Polymerase Chain Reaction:

    Article Title: RNA sequencing reveals differential expression of circular RNAs in human small cell lung cancer
    Article Snippet: .. The PCR product was purified from agarose gel, restriction digested, and ligated into the pcDNA3.1 (+) circRNA mini vector (#60648, Addgene) using T4 DNA ligase (NEB). ..

    Article Title: RNA sequencing reveals differential expression of circular RNAs in human small cell lung cancer.
    Article Snippet: .. The PCR product was purified from agarose gel, restriction digested, and ligated into the pcDNA3.1(+) circRNA mini vector (#60648, Addgene) using T4 DNA ligase (NEB). ..

    Purification:

    Article Title: RNA sequencing reveals differential expression of circular RNAs in human small cell lung cancer
    Article Snippet: .. The PCR product was purified from agarose gel, restriction digested, and ligated into the pcDNA3.1 (+) circRNA mini vector (#60648, Addgene) using T4 DNA ligase (NEB). ..

    Article Title: RNA sequencing reveals differential expression of circular RNAs in human small cell lung cancer.
    Article Snippet: .. The PCR product was purified from agarose gel, restriction digested, and ligated into the pcDNA3.1(+) circRNA mini vector (#60648, Addgene) using T4 DNA ligase (NEB). ..

    Agarose Gel Electrophoresis:

    Article Title: RNA sequencing reveals differential expression of circular RNAs in human small cell lung cancer
    Article Snippet: .. The PCR product was purified from agarose gel, restriction digested, and ligated into the pcDNA3.1 (+) circRNA mini vector (#60648, Addgene) using T4 DNA ligase (NEB). ..

    Article Title: RNA sequencing reveals differential expression of circular RNAs in human small cell lung cancer.
    Article Snippet: .. The PCR product was purified from agarose gel, restriction digested, and ligated into the pcDNA3.1(+) circRNA mini vector (#60648, Addgene) using T4 DNA ligase (NEB). ..

    Plasmid Preparation:

    Article Title: RNA sequencing reveals differential expression of circular RNAs in human small cell lung cancer
    Article Snippet: .. The PCR product was purified from agarose gel, restriction digested, and ligated into the pcDNA3.1 (+) circRNA mini vector (#60648, Addgene) using T4 DNA ligase (NEB). ..

    Article Title: Circular RNA hsa_circ_0099188 Regulates Inducible Nitric Oxide Synthase and Chemokine Transcription in Macrophages by Targeting the hsa-miR-381-3p/PPP3CA and hsa-miR-381-3p/KLF4 Pathways in Response to 4,4’-Methylene Diphenyl Diisocyanate-Glutathione Conjugate Exposure
    Article Snippet: .. The circRNA overexpression vector, pcDNA3.1 (+) _CircRNA_Mini Vector, was obtained from Addgene (plasmid id#60648; Watertown, MA), deposited by Dr. Jeremy Wilusz at Baylor College of Medicine ( Liang and Wilusz 2014 ). ..

    Article Title: CircNFATc3 promotes Fetal Hemoglobin induction by regulating let-7b/GATA2 axis
    Article Snippet: Images of the stained cells were taken using Nikon TE2000 microscope equipped with a digital camera. .. The pcDNA3.1(+) CircRNA Mini Vector was a gift from Jeremy Wilusz (Addgene plasmid #60648; http://n2t.net/addgene:60648 ; RRID: Addgene_60648)[ ]. .. For overexpression of circNFATc3, the cDNA of NFATc3 exons 2 and 3 were amplified from K562 cells and cloned into pcDNA3.1(+) CircRNA Mini Vector and pcDNA3.1(+) empty vector (Supplementary Table S1).

    Article Title: RNA sequencing reveals differential expression of circular RNAs in human small cell lung cancer.
    Article Snippet: .. The PCR product was purified from agarose gel, restriction digested, and ligated into the pcDNA3.1(+) circRNA mini vector (#60648, Addgene) using T4 DNA ligase (NEB). ..



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