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human cell stress array kit  (R&D Systems)


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    R&D Systems human cell stress array kit
    ( A ) Wright-Giemsa staining of <t>cells</t> after 5-day treatment [Cis (0.45 μg/ml) and Dau (0.175 μg/ml)]. ( B ) Immunofluorescence staining of CD14 and the statistical data of the nucleus areas stained by DAPI. Scale bar, 20 μm. ( C ) Cathepsin B evaluation by a Magic Red assay <t>kit</t> and the relative integrated density of Magic Red in cytoplasm. Scale bar, 10 μm. ( D ) FACS analysis of the CD14 expression with or without NAC pretreatment ( n = 3). ( E ) Relative ROS in Kasumi-1 cells incubated with Cis + Dau and HA/Cis/Dau with or without NAC pretreatment ( n = 3). ( F ) Proteome profiler <t>array.</t> “*,” “#,” or “&” P < 0.05; “**,” “##,” or “&&” P < 0.01; *** P < 0.001; **** P < 0.0001. Con, control.
    Human Cell Stress Array Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cell+stress+array+kit/pmc11952094-260-2-7?v=R%26D+Systems
    Average 93 stars, based on 16 article reviews
    human cell stress array kit - by Bioz Stars, 2026-08
    93/100 stars

    Images

    1) Product Images from "A dual chemodrug-loaded hyaluronan nanogel for differentiation induction therapy of refractory AML via disrupting lysosomal homeostasis"

    Article Title: A dual chemodrug-loaded hyaluronan nanogel for differentiation induction therapy of refractory AML via disrupting lysosomal homeostasis

    Journal: Science Advances

    doi: 10.1126/sciadv.ado3923

    ( A ) Wright-Giemsa staining of cells after 5-day treatment [Cis (0.45 μg/ml) and Dau (0.175 μg/ml)]. ( B ) Immunofluorescence staining of CD14 and the statistical data of the nucleus areas stained by DAPI. Scale bar, 20 μm. ( C ) Cathepsin B evaluation by a Magic Red assay kit and the relative integrated density of Magic Red in cytoplasm. Scale bar, 10 μm. ( D ) FACS analysis of the CD14 expression with or without NAC pretreatment ( n = 3). ( E ) Relative ROS in Kasumi-1 cells incubated with Cis + Dau and HA/Cis/Dau with or without NAC pretreatment ( n = 3). ( F ) Proteome profiler array. “*,” “#,” or “&” P < 0.05; “**,” “##,” or “&&” P < 0.01; *** P < 0.001; **** P < 0.0001. Con, control.
    Figure Legend Snippet: ( A ) Wright-Giemsa staining of cells after 5-day treatment [Cis (0.45 μg/ml) and Dau (0.175 μg/ml)]. ( B ) Immunofluorescence staining of CD14 and the statistical data of the nucleus areas stained by DAPI. Scale bar, 20 μm. ( C ) Cathepsin B evaluation by a Magic Red assay kit and the relative integrated density of Magic Red in cytoplasm. Scale bar, 10 μm. ( D ) FACS analysis of the CD14 expression with or without NAC pretreatment ( n = 3). ( E ) Relative ROS in Kasumi-1 cells incubated with Cis + Dau and HA/Cis/Dau with or without NAC pretreatment ( n = 3). ( F ) Proteome profiler array. “*,” “#,” or “&” P < 0.05; “**,” “##,” or “&&” P < 0.01; *** P < 0.001; **** P < 0.0001. Con, control.

    Techniques Used: Staining, Immunofluorescence, Expressing, Incubation, Control



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    Phosphorylation <t>of</t> <t>receptor</t> tyrosine kinases (RTKs) and their downstream effectors in human follicle dermal papilla cells treated with a Silybum marianum extract (SME, 30 μg/mL, dissolved in dimethylsulfoxide [DMSO]) or DMSO for 1 h ( n = 2 donors, tests performed in duplicate for DMSO‐treated and SME‐treated cells from each donor). (a) EGFR and PDGFRβ, and (b) the downstream phosphokinases ERK1/2, GSK3α/β, Akt1/2/3, STAT5α and STAT5α/β. Data were obtained using the Human <t>Proteome</t> Profiler Phospho‐RTK and Phospho‐Kinase array kits, respectively. Each bar represents the mean ± SEM of SME/DMSO ratio.
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    Image Search Results


    ( A ) Wright-Giemsa staining of cells after 5-day treatment [Cis (0.45 μg/ml) and Dau (0.175 μg/ml)]. ( B ) Immunofluorescence staining of CD14 and the statistical data of the nucleus areas stained by DAPI. Scale bar, 20 μm. ( C ) Cathepsin B evaluation by a Magic Red assay kit and the relative integrated density of Magic Red in cytoplasm. Scale bar, 10 μm. ( D ) FACS analysis of the CD14 expression with or without NAC pretreatment ( n = 3). ( E ) Relative ROS in Kasumi-1 cells incubated with Cis + Dau and HA/Cis/Dau with or without NAC pretreatment ( n = 3). ( F ) Proteome profiler array. “*,” “#,” or “&” P < 0.05; “**,” “##,” or “&&” P < 0.01; *** P < 0.001; **** P < 0.0001. Con, control.

    Journal: Science Advances

    Article Title: A dual chemodrug-loaded hyaluronan nanogel for differentiation induction therapy of refractory AML via disrupting lysosomal homeostasis

    doi: 10.1126/sciadv.ado3923

    Figure Lengend Snippet: ( A ) Wright-Giemsa staining of cells after 5-day treatment [Cis (0.45 μg/ml) and Dau (0.175 μg/ml)]. ( B ) Immunofluorescence staining of CD14 and the statistical data of the nucleus areas stained by DAPI. Scale bar, 20 μm. ( C ) Cathepsin B evaluation by a Magic Red assay kit and the relative integrated density of Magic Red in cytoplasm. Scale bar, 10 μm. ( D ) FACS analysis of the CD14 expression with or without NAC pretreatment ( n = 3). ( E ) Relative ROS in Kasumi-1 cells incubated with Cis + Dau and HA/Cis/Dau with or without NAC pretreatment ( n = 3). ( F ) Proteome profiler array. “*,” “#,” or “&” P < 0.05; “**,” “##,” or “&&” P < 0.01; *** P < 0.001; **** P < 0.0001. Con, control.

    Article Snippet: Then, the Human Cell Stress Array Kit (R&D Systems, USA) was used to detect the differences among different treatment groups.

    Techniques: Staining, Immunofluorescence, Expressing, Incubation, Control

    Phosphorylation of receptor tyrosine kinases (RTKs) and their downstream effectors in human follicle dermal papilla cells treated with a Silybum marianum extract (SME, 30 μg/mL, dissolved in dimethylsulfoxide [DMSO]) or DMSO for 1 h ( n = 2 donors, tests performed in duplicate for DMSO‐treated and SME‐treated cells from each donor). (a) EGFR and PDGFRβ, and (b) the downstream phosphokinases ERK1/2, GSK3α/β, Akt1/2/3, STAT5α and STAT5α/β. Data were obtained using the Human Proteome Profiler Phospho‐RTK and Phospho‐Kinase array kits, respectively. Each bar represents the mean ± SEM of SME/DMSO ratio.

    Journal: Journal of Cosmetic Dermatology

    Article Title: New Plant Extracts Exert Complementary Anti‐Hair Loss Properties in Human In Vitro and Ex Vivo Models

    doi: 10.1111/jocd.16616

    Figure Lengend Snippet: Phosphorylation of receptor tyrosine kinases (RTKs) and their downstream effectors in human follicle dermal papilla cells treated with a Silybum marianum extract (SME, 30 μg/mL, dissolved in dimethylsulfoxide [DMSO]) or DMSO for 1 h ( n = 2 donors, tests performed in duplicate for DMSO‐treated and SME‐treated cells from each donor). (a) EGFR and PDGFRβ, and (b) the downstream phosphokinases ERK1/2, GSK3α/β, Akt1/2/3, STAT5α and STAT5α/β. Data were obtained using the Human Proteome Profiler Phospho‐RTK and Phospho‐Kinase array kits, respectively. Each bar represents the mean ± SEM of SME/DMSO ratio.

    Article Snippet: Growth factor receptor (GFR) signaling pathways were analyzed using two Human Proteome Profiler antibody array kits (R&D Systems, USA) to simultaneously detect the phosphorylation of 49 receptor tyrosine kinases (Proteome Profiler Human Phospho‐RTK Array Kit) and of 43 kinases and two related total proteins (Proteome Profiler Human Phospho‐Kinase Array Kit).

    Techniques: Phospho-proteomics