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cytometric bead array for human apoptosis cba  (Becton Dickinson)

 
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    Becton Dickinson cytometric bead array for human apoptosis cba
    Time course of <t>apoptosis</t> and necrosis of post-hypoxic neuronal cells in direct co-cultures with tMNC, CD133 + and CD133 - . Box plots showing time course of apoptosis (A) and necrosis/late apoptosis (B) in direct co-cultures of post-hypoxic neuronal cells with tMNC, CD133 - or CD133 + (colored plots). Grey boxes display the development of cell death in post-hypoxic neuronal cells directly after hypoxic pre-cultivation of SH-SY5Y cells (Day 0) and 1–3 days post-hypoxia. The ordinate represents the percentage of apoptotic and necrotic cells, respectively. Rates of apoptosis and necrosis were calculated in relation to the total number of SH-SY5Y cells.
    Cytometric Bead Array For Human Apoptosis Cba, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+apoptosis+array/cytometric+bead+array+for+human+apoptosis+cba/pmc02569042-158-4-7
    Average 90 stars, based on 1 article reviews
    cytometric bead array for human apoptosis cba - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Neuronal hypoxia in vitro : Investigation of therapeutic principles of HUCB-MNC and CD133 + stem cells"

    Article Title: Neuronal hypoxia in vitro : Investigation of therapeutic principles of HUCB-MNC and CD133 + stem cells

    Journal: BMC Neuroscience

    doi: 10.1186/1471-2202-9-91

    Time course of apoptosis and necrosis of post-hypoxic neuronal cells in direct co-cultures with tMNC, CD133 + and CD133 - . Box plots showing time course of apoptosis (A) and necrosis/late apoptosis (B) in direct co-cultures of post-hypoxic neuronal cells with tMNC, CD133 - or CD133 + (colored plots). Grey boxes display the development of cell death in post-hypoxic neuronal cells directly after hypoxic pre-cultivation of SH-SY5Y cells (Day 0) and 1–3 days post-hypoxia. The ordinate represents the percentage of apoptotic and necrotic cells, respectively. Rates of apoptosis and necrosis were calculated in relation to the total number of SH-SY5Y cells.
    Figure Legend Snippet: Time course of apoptosis and necrosis of post-hypoxic neuronal cells in direct co-cultures with tMNC, CD133 + and CD133 - . Box plots showing time course of apoptosis (A) and necrosis/late apoptosis (B) in direct co-cultures of post-hypoxic neuronal cells with tMNC, CD133 - or CD133 + (colored plots). Grey boxes display the development of cell death in post-hypoxic neuronal cells directly after hypoxic pre-cultivation of SH-SY5Y cells (Day 0) and 1–3 days post-hypoxia. The ordinate represents the percentage of apoptotic and necrotic cells, respectively. Rates of apoptosis and necrosis were calculated in relation to the total number of SH-SY5Y cells.

    Techniques Used:

    Effects of direct and indirect application of tMNC and CD133 - on apoptosis and necrosis of post-hypoxic neuronal cells . Box plots represent the percentage of annexin-V positive post-hypoxic neuronal cells (A, B) and of PI positive post-hypoxic neuronal cells (C, D) in direct and indirect co-cultures. Co-cultures were performed with tMNC (A, C) and CD133 - (B, D). The extents of apoptosis (annexin-V-binding) and necrosis (PI) were analyzed for three days after hypoxia.
    Figure Legend Snippet: Effects of direct and indirect application of tMNC and CD133 - on apoptosis and necrosis of post-hypoxic neuronal cells . Box plots represent the percentage of annexin-V positive post-hypoxic neuronal cells (A, B) and of PI positive post-hypoxic neuronal cells (C, D) in direct and indirect co-cultures. Co-cultures were performed with tMNC (A, C) and CD133 - (B, D). The extents of apoptosis (annexin-V-binding) and necrosis (PI) were analyzed for three days after hypoxia.

    Techniques Used: Binding Assay

    Direct comparison of effects of tMNC and CD133 - on late apoptosis (PARP cleavage) . Bar chart showing concentrations of cleaved PARP in lysates of post-hypoxic neuronal cells which were indirectly co-cultivated with tMNC (grey shaded bars) or CD133 - (black bars). Controls are post-hypoxic mono-cultures (grey bars) and normoxic cultures (white bars). Each bar visualizes pooled samples obtained from three independent experiments.
    Figure Legend Snippet: Direct comparison of effects of tMNC and CD133 - on late apoptosis (PARP cleavage) . Bar chart showing concentrations of cleaved PARP in lysates of post-hypoxic neuronal cells which were indirectly co-cultivated with tMNC (grey shaded bars) or CD133 - (black bars). Controls are post-hypoxic mono-cultures (grey bars) and normoxic cultures (white bars). Each bar visualizes pooled samples obtained from three independent experiments.

    Techniques Used: Comparison

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    Article Title: Neuronal hypoxia in vitro : Investigation of therapeutic principles of HUCB-MNC and CD133 + stem cells
    Article Snippet: .. Cytometric Bead Array for human apoptosis (CBA, Becton Dickinson, Erembodegem, Belgium) was used to quantify the apoptosis specific parameter cleaved Poly-ADP-Ribose-Polymerase I (PARP) in lysates of post-hypoxic neuronal cells after indirect co-cultivation with tMNC and CD133 - . .. For indirect co-cultivation tMNC or CD133 - were added in cell impassable cell culture inserts with a pore size of 0.4 μm (Greiner Bio-One GmbH, Frickenhausen, Germany).



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    Effects of benjaminin on apoptosis activation and apoptotic protein levels following 24 h of treatment with 0.1% DMSO (control) or benjaminin (IC₅₀). Benjaminin increased the proportion of apoptotic cells and modulated apoptotic protein expression. ( a ) Representative cytograms showing cell distribution across apoptotic stages. ( b ) Fold changes in apoptotic protein signal intensity relative to DMSO; lysates were pooled from two independent experiments. Data in ( a ) are mean ± SD of three biological replicates (n = 3). Statistical significance was determined using Student’s t-test. *P < 0.05, **P < 0.001.

    Journal: Scientific Reports

    Article Title: A natural coumarin, benjaminin, suppresses proliferation and migration and induces apoptosis in MCF-7 breast cancer cells

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    Figure Lengend Snippet: Effects of benjaminin on apoptosis activation and apoptotic protein levels following 24 h of treatment with 0.1% DMSO (control) or benjaminin (IC₅₀). Benjaminin increased the proportion of apoptotic cells and modulated apoptotic protein expression. ( a ) Representative cytograms showing cell distribution across apoptotic stages. ( b ) Fold changes in apoptotic protein signal intensity relative to DMSO; lysates were pooled from two independent experiments. Data in ( a ) are mean ± SD of three biological replicates (n = 3). Statistical significance was determined using Student’s t-test. *P < 0.05, **P < 0.001.

    Article Snippet: RayBio® Human Apoptosis Array G1 kit was purchased from Raybiotech (USA).

    Techniques: Activation Assay, Control, Expressing

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    Figure Lengend Snippet: Trichodermamide B (TRH2) affects prostate cell cycle and apoptosis in prostate cancer cells. A & B Effect of TRH2 on the cell cycle distribution of LNCaP cells. C & D Effect of TRH2 on the cell cycle distribution of PC-3 cells. E & F The protein expression of Cyclin A2 in LNCaP cells. G & H The protein expression of Cyclin A2 in PC-3 cells. I & J Effect of TRH2 on apoptosis in 22Rv1 cells. K & L Effect of TRH2 on apoptosis in PC-3 cells. * P <0.05, ** P <0.01 vs control

    Article Snippet: After treatment with either DMSO (0.1%, v/v) or TRH2 (8 μM) for 48 h, cells were harvested and proteins were extracted and analyzed according to the instructions of the Human Apoptosis Array Kit (Cat# ARY009, R&D Systems).

    Techniques: Expressing, Control

    Trichodermamide B (TRH2) alters the ultrastructure of prostate cancer cells and modifies the expression of apoptosis-related proteins. A Morphology of normal LNCaP cells under the transmission electron microscope. B & C Morphological changes in LNCaP cells treated with TRH2 under transmission electron microscopy. D & E Effect of TRH2 on the expression of apoptosis-related proteins in LNCaP cells. F & G Effect of TRH2 on the expression of apoptosis-related proteins in PC-3 cells

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    Figure Lengend Snippet: Trichodermamide B (TRH2) alters the ultrastructure of prostate cancer cells and modifies the expression of apoptosis-related proteins. A Morphology of normal LNCaP cells under the transmission electron microscope. B & C Morphological changes in LNCaP cells treated with TRH2 under transmission electron microscopy. D & E Effect of TRH2 on the expression of apoptosis-related proteins in LNCaP cells. F & G Effect of TRH2 on the expression of apoptosis-related proteins in PC-3 cells

    Article Snippet: After treatment with either DMSO (0.1%, v/v) or TRH2 (8 μM) for 48 h, cells were harvested and proteins were extracted and analyzed according to the instructions of the Human Apoptosis Array Kit (Cat# ARY009, R&D Systems).

    Techniques: Expressing, Transmission Assay, Microscopy, Electron Microscopy