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human apoptosis array kit  (R&D Systems)


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    R&D Systems human apoptosis array kit
    Trichodermamide B (TRH2) affects prostate cell cycle and <t>apoptosis</t> in prostate cancer cells. A & B Effect of TRH2 on the cell cycle distribution of LNCaP cells. C & D Effect of TRH2 on the cell cycle distribution of PC-3 cells. E & F The protein expression of Cyclin A2 in LNCaP cells. G & H The protein expression of Cyclin A2 in PC-3 cells. I & J Effect of TRH2 on apoptosis in 22Rv1 cells. K & L Effect of TRH2 on apoptosis in PC-3 cells. * P <0.05, ** P <0.01 vs control
    Human Apoptosis Array Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 251 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+apoptosis+array+kit/Proteome+Profiler+Human+Apoptosis+Array+Kit/pmc13014934-92-29-35
    Average 96 stars, based on 251 article reviews
    human apoptosis array kit - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Marine trichodermamide B inhibits prostate cancer progression via catalase inhibition-induced apoptosis"

    Article Title: Marine trichodermamide B inhibits prostate cancer progression via catalase inhibition-induced apoptosis

    Journal: Cell Communication and Signaling : CCS

    doi: 10.1186/s12964-026-02737-y

    Trichodermamide B (TRH2) affects prostate cell cycle and apoptosis in prostate cancer cells. A & B Effect of TRH2 on the cell cycle distribution of LNCaP cells. C & D Effect of TRH2 on the cell cycle distribution of PC-3 cells. E & F The protein expression of Cyclin A2 in LNCaP cells. G & H The protein expression of Cyclin A2 in PC-3 cells. I & J Effect of TRH2 on apoptosis in 22Rv1 cells. K & L Effect of TRH2 on apoptosis in PC-3 cells. * P <0.05, ** P <0.01 vs control
    Figure Legend Snippet: Trichodermamide B (TRH2) affects prostate cell cycle and apoptosis in prostate cancer cells. A & B Effect of TRH2 on the cell cycle distribution of LNCaP cells. C & D Effect of TRH2 on the cell cycle distribution of PC-3 cells. E & F The protein expression of Cyclin A2 in LNCaP cells. G & H The protein expression of Cyclin A2 in PC-3 cells. I & J Effect of TRH2 on apoptosis in 22Rv1 cells. K & L Effect of TRH2 on apoptosis in PC-3 cells. * P <0.05, ** P <0.01 vs control

    Techniques Used: Expressing, Control

    Trichodermamide B (TRH2) alters the ultrastructure of prostate cancer cells and modifies the expression of apoptosis-related proteins. A Morphology of normal LNCaP cells under the transmission electron microscope. B & C Morphological changes in LNCaP cells treated with TRH2 under transmission electron microscopy. D & E Effect of TRH2 on the expression of apoptosis-related proteins in LNCaP cells. F & G Effect of TRH2 on the expression of apoptosis-related proteins in PC-3 cells
    Figure Legend Snippet: Trichodermamide B (TRH2) alters the ultrastructure of prostate cancer cells and modifies the expression of apoptosis-related proteins. A Morphology of normal LNCaP cells under the transmission electron microscope. B & C Morphological changes in LNCaP cells treated with TRH2 under transmission electron microscopy. D & E Effect of TRH2 on the expression of apoptosis-related proteins in LNCaP cells. F & G Effect of TRH2 on the expression of apoptosis-related proteins in PC-3 cells

    Techniques Used: Expressing, Transmission Assay, Microscopy, Electron Microscopy

    Related Articles

    Expressing:

    Article Title: Enhanced Payload Release Enables Disitamab Vedotin to Surpass Trastuzumab Emtansine and Retain Efficacy in Acquired Resistance to Clinical Anti-HER2 Therapies
    Article Snippet: After incubation, samples (50,000 cells) were acquired in an Accuri C6 Flow Cytometer (BD Biosciences), considering viable if they were negative for both Annexin V, which detects early apoptotic cells, and PI, which identifies necrotic cells. .. To analyze the expression profiles of apoptosis-related proteins, the Proteome Profiler Human Apoptosis Array Kit (R&D Systems, Minneapolis, USA) was used according to the manufacturer’s instructions. ..

    Article Title: Erica spiculifolia Extract Potentiates Cisplatin Cytotoxicity by Reactivating p53 and Caspase-3-Dependent Apoptosis in Colorectal Carcinoma.
    Article Snippet: .. Protein expression profiling was carried out using membrane-based sandwich immunoassays according to the manufacturer’s instructions (Human Apoptosis Array Kit, ARY009; R&D Systems, Minneapolis, MN, USA). .. Signal intensities of individual protein spots were quantified by densitometric analysis using ImageJ software (version 1.8.0) and subsequently converted into absolute protein expression values.

    Article Title: Erica spiculifolia Extract Potentiates Cisplatin Cytotoxicity by Reactivating p53 and Caspase-3-Dependent Apoptosis in Colorectal Carcinoma
    Article Snippet: .. Protein expression profiling was carried out using membrane-based sandwich immunoassays according to the manufacturer’s instructions (Human Apoptosis Array Kit, ARY009; R&D Systems, Minneapolis, MN, USA). .. Signal intensities of individual protein spots were quantified by densitometric analysis using ImageJ software (version 1.8.0) and subsequently converted into absolute protein expression values.

    Article Title: Enhanced Payload Release Enables Disitamab Vedotin to Surpass Trastuzumab Emtansine and Retain Efficacy in Acquired Resistance to Clinical Anti-HER2 Therapies.
    Article Snippet: After incubation, samples (50,000 cells) were acquired in an Accuri C6 Flow Cytometer (BD Biosciences), considering viable if they were negative for both Annexin V, which detects early apoptotic cells, and PI, which identifies necrotic cells. .. To analyze the expression profiles of apoptosis-related proteins, the Proteome Profiler Human Apoptosis Array Kit (R&D Systems, Minneapolis, USA) was used according to the manufacturer’s instructions. ..

    Membrane:

    Article Title: Erica spiculifolia Extract Potentiates Cisplatin Cytotoxicity by Reactivating p53 and Caspase-3-Dependent Apoptosis in Colorectal Carcinoma.
    Article Snippet: .. Protein expression profiling was carried out using membrane-based sandwich immunoassays according to the manufacturer’s instructions (Human Apoptosis Array Kit, ARY009; R&D Systems, Minneapolis, MN, USA). .. Signal intensities of individual protein spots were quantified by densitometric analysis using ImageJ software (version 1.8.0) and subsequently converted into absolute protein expression values.

    Article Title: Erica spiculifolia Extract Potentiates Cisplatin Cytotoxicity by Reactivating p53 and Caspase-3-Dependent Apoptosis in Colorectal Carcinoma
    Article Snippet: .. Protein expression profiling was carried out using membrane-based sandwich immunoassays according to the manufacturer’s instructions (Human Apoptosis Array Kit, ARY009; R&D Systems, Minneapolis, MN, USA). .. Signal intensities of individual protein spots were quantified by densitometric analysis using ImageJ software (version 1.8.0) and subsequently converted into absolute protein expression values.

    Imaging:

    Article Title: Functionalized nanoparticle transforms “cold” to “hot” adenoid cystic carcinoma of salivary gland tumour microenvironment in vitro
    Article Snippet: .. Upon following manufacturer instruction of Proteome Profiler Human Apoptosis Array Kit (R&D Systems, Cat RDSARY009), high-resolution images were acquired using Chemidoc MP imaging system and processed using Image Lab version 6.0 (Bio-Rad Laboratories, Hercules, CA, USA). .. Quantification of relative protein expressions were done using microarray profile plugin in ImageJ (ImageJ 1.52a, National Institutes of Health, USA).

    other:

    Article Title: Marine trichodermamide B inhibits prostate cancer progression via catalase inhibition-induced apoptosis
    Article Snippet: After treatment with either DMSO (0.1%, v/v) or TRH2 (8 μM) for 48 h, cells were harvested and proteins were extracted and analyzed according to the instructions of the Human Apoptosis Array Kit (Cat# ARY009, R&D Systems).

    Article Title: Marine trichodermamide B inhibits prostate cancer progression via catalase inhibition-induced apoptosis.
    Article Snippet: Apoptosis and cell cycle distribution were analyzed by flow cytometry (NovoCyte, Agilent) as previously described (Gan et al., 2024).

    Lysis:

    Article Title: Functionalized nanoparticle transforms “cold” to “hot” adenoid cystic carcinoma of salivary gland tumour microenvironment in vitro
    Article Snippet: .. The adenoid cystic carcinoma cells were added to 100 μL of lysis solution, supplied in Proteome Profiler Human Apoptosis Array Kit (R&D Systems, Cat RDSARY009) and 10 μL of protease inhibitor (Protease Inhibitor Cocktail I, Tocris, Cat 5500). ..

    Protease Inhibitor:

    Article Title: Functionalized nanoparticle transforms “cold” to “hot” adenoid cystic carcinoma of salivary gland tumour microenvironment in vitro
    Article Snippet: .. The adenoid cystic carcinoma cells were added to 100 μL of lysis solution, supplied in Proteome Profiler Human Apoptosis Array Kit (R&D Systems, Cat RDSARY009) and 10 μL of protease inhibitor (Protease Inhibitor Cocktail I, Tocris, Cat 5500). ..



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    Image Search Results


    Effects of benjaminin on apoptosis activation and apoptotic protein levels following 24 h of treatment with 0.1% DMSO (control) or benjaminin (IC₅₀). Benjaminin increased the proportion of apoptotic cells and modulated apoptotic protein expression. ( a ) Representative cytograms showing cell distribution across apoptotic stages. ( b ) Fold changes in apoptotic protein signal intensity relative to DMSO; lysates were pooled from two independent experiments. Data in ( a ) are mean ± SD of three biological replicates (n = 3). Statistical significance was determined using Student’s t-test. *P < 0.05, **P < 0.001.

    Journal: Scientific Reports

    Article Title: A natural coumarin, benjaminin, suppresses proliferation and migration and induces apoptosis in MCF-7 breast cancer cells

    doi: 10.1038/s41598-026-51303-6

    Figure Lengend Snippet: Effects of benjaminin on apoptosis activation and apoptotic protein levels following 24 h of treatment with 0.1% DMSO (control) or benjaminin (IC₅₀). Benjaminin increased the proportion of apoptotic cells and modulated apoptotic protein expression. ( a ) Representative cytograms showing cell distribution across apoptotic stages. ( b ) Fold changes in apoptotic protein signal intensity relative to DMSO; lysates were pooled from two independent experiments. Data in ( a ) are mean ± SD of three biological replicates (n = 3). Statistical significance was determined using Student’s t-test. *P < 0.05, **P < 0.001.

    Article Snippet: RayBio® Human Apoptosis Array G1 kit was purchased from Raybiotech (USA).

    Techniques: Activation Assay, Control, Expressing

    Trichodermamide B (TRH2) affects prostate cell cycle and apoptosis in prostate cancer cells. A & B Effect of TRH2 on the cell cycle distribution of LNCaP cells. C & D Effect of TRH2 on the cell cycle distribution of PC-3 cells. E & F The protein expression of Cyclin A2 in LNCaP cells. G & H The protein expression of Cyclin A2 in PC-3 cells. I & J Effect of TRH2 on apoptosis in 22Rv1 cells. K & L Effect of TRH2 on apoptosis in PC-3 cells. * P <0.05, ** P <0.01 vs control

    Journal: Cell Communication and Signaling : CCS

    Article Title: Marine trichodermamide B inhibits prostate cancer progression via catalase inhibition-induced apoptosis

    doi: 10.1186/s12964-026-02737-y

    Figure Lengend Snippet: Trichodermamide B (TRH2) affects prostate cell cycle and apoptosis in prostate cancer cells. A & B Effect of TRH2 on the cell cycle distribution of LNCaP cells. C & D Effect of TRH2 on the cell cycle distribution of PC-3 cells. E & F The protein expression of Cyclin A2 in LNCaP cells. G & H The protein expression of Cyclin A2 in PC-3 cells. I & J Effect of TRH2 on apoptosis in 22Rv1 cells. K & L Effect of TRH2 on apoptosis in PC-3 cells. * P <0.05, ** P <0.01 vs control

    Article Snippet: After treatment with either DMSO (0.1%, v/v) or TRH2 (8 μM) for 48 h, cells were harvested and proteins were extracted and analyzed according to the instructions of the Human Apoptosis Array Kit (Cat# ARY009, R&D Systems).

    Techniques: Expressing, Control

    Trichodermamide B (TRH2) alters the ultrastructure of prostate cancer cells and modifies the expression of apoptosis-related proteins. A Morphology of normal LNCaP cells under the transmission electron microscope. B & C Morphological changes in LNCaP cells treated with TRH2 under transmission electron microscopy. D & E Effect of TRH2 on the expression of apoptosis-related proteins in LNCaP cells. F & G Effect of TRH2 on the expression of apoptosis-related proteins in PC-3 cells

    Journal: Cell Communication and Signaling : CCS

    Article Title: Marine trichodermamide B inhibits prostate cancer progression via catalase inhibition-induced apoptosis

    doi: 10.1186/s12964-026-02737-y

    Figure Lengend Snippet: Trichodermamide B (TRH2) alters the ultrastructure of prostate cancer cells and modifies the expression of apoptosis-related proteins. A Morphology of normal LNCaP cells under the transmission electron microscope. B & C Morphological changes in LNCaP cells treated with TRH2 under transmission electron microscopy. D & E Effect of TRH2 on the expression of apoptosis-related proteins in LNCaP cells. F & G Effect of TRH2 on the expression of apoptosis-related proteins in PC-3 cells

    Article Snippet: After treatment with either DMSO (0.1%, v/v) or TRH2 (8 μM) for 48 h, cells were harvested and proteins were extracted and analyzed according to the instructions of the Human Apoptosis Array Kit (Cat# ARY009, R&D Systems).

    Techniques: Expressing, Transmission Assay, Microscopy, Electron Microscopy