proteome profiler human apoptosis array kit a kim (R&D Systems)

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R&D Systems proteome profiler human apoptosis array kit a kim

Fig. 3. Increase of caspase activities by CAA treatment in iPSCs. (A) <t>Apoptosis-related</t> proteins were detected by Western blotting in CAA-treated iPSCs and hDFs. Relative band intensities were calculated after normalization to β-actin expression. (B) iPSCs and hDFs were treated with 50 and 100 μM CAA for 24 h, and the caspase-3, −8, and −9 activities were determined. Relative caspase activity compared with CAA-untreated iPSCs and hDFs was expressed as the means ± SD (n = 3). (C) iPSCs were pre-treated with or without 10 μM of caspase-3 inhibitor (z-DEVD), caspase-8 inhibitor (z-IETD), caspase-9 inhibitor (z-LEHD), and pan-caspase inhibitor (z-VAD) for 30 min and then treated with 50 and 100 μM CAA. DMSO was treated as vehicle control. After 24 h, cells were stained with crystal violet solution and relative cell viability compared with CAA-untreated control iPSCs were expressed as the means ± SD (n = 3). **p < 0.01 vs. CAA-untreated control. ##p < 0.01 vs. Inhibitor-untreated control. Scale bar = 100 μM.

Proteome Profiler Human Apoptosis Array Kit A Kim, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+apoptosis+array+kit/Proteome+Profiler+Human+Apoptosis+Array+Kit/pm35537368-70-16-31
Average 96 stars, based on 247 article reviews

proteome profiler human apoptosis array kit a kim - by Bioz Stars, 2026-09

96/100 stars

Images

1) Product Images from "Caffeic acid selectively eliminates teratogenic human-induced pluripotent stem cells via apoptotic cell death."

Article Title: Caffeic acid selectively eliminates teratogenic human-induced pluripotent stem cells via apoptotic cell death.

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

doi: 10.1016/j.phymed.2022.154144

Fig. 3. Increase of caspase activities by CAA treatment in iPSCs. (A) Apoptosis-related proteins were detected by Western blotting in CAA-treated iPSCs and hDFs. Relative band intensities were calculated after normalization to β-actin expression. (B) iPSCs and hDFs were treated with 50 and 100 μM CAA for 24 h, and the caspase-3, −8, and −9 activities were determined. Relative caspase activity compared with CAA-untreated iPSCs and hDFs was expressed as the means ± SD (n = 3). (C) iPSCs were pre-treated with or without 10 μM of caspase-3 inhibitor (z-DEVD), caspase-8 inhibitor (z-IETD), caspase-9 inhibitor (z-LEHD), and pan-caspase inhibitor (z-VAD) for 30 min and then treated with 50 and 100 μM CAA. DMSO was treated as vehicle control. After 24 h, cells were stained with crystal violet solution and relative cell viability compared with CAA-untreated control iPSCs were expressed as the means ± SD (n = 3). **p < 0.01 vs. CAA-untreated control. ##p < 0.01 vs. Inhibitor-untreated control. Scale bar = 100 μM.

Figure Legend Snippet: Fig. 3. Increase of caspase activities by CAA treatment in iPSCs. (A) Apoptosis-related proteins were detected by Western blotting in CAA-treated iPSCs and hDFs. Relative band intensities were calculated after normalization to β-actin expression. (B) iPSCs and hDFs were treated with 50 and 100 μM CAA for 24 h, and the caspase-3, −8, and −9 activities were determined. Relative caspase activity compared with CAA-untreated iPSCs and hDFs was expressed as the means ± SD (n = 3). (C) iPSCs were pre-treated with or without 10 μM of caspase-3 inhibitor (z-DEVD), caspase-8 inhibitor (z-IETD), caspase-9 inhibitor (z-LEHD), and pan-caspase inhibitor (z-VAD) for 30 min and then treated with 50 and 100 μM CAA. DMSO was treated as vehicle control. After 24 h, cells were stained with crystal violet solution and relative cell viability compared with CAA-untreated control iPSCs were expressed as the means ± SD (n = 3). **p < 0.01 vs. CAA-untreated control. ##p < 0.01 vs. Inhibitor-untreated control. Scale bar = 100 μM.

Techniques Used: Western Blot, Expressing, Activity Assay, Control, Staining

Expressing:

Article Title: Enhanced Payload Release Enables Disitamab Vedotin to Surpass Trastuzumab Emtansine and Retain Efficacy in Acquired Resistance to Clinical Anti-HER2 Therapies
Article Snippet: After incubation, samples (50,000 cells) were acquired in an Accuri C6 Flow Cytometer (BD Biosciences), considering viable if they were negative for both Annexin V, which detects early apoptotic cells, and PI, which identifies necrotic cells. .. To analyze the expression profiles of apoptosis-related proteins, the Proteome Profiler Human Apoptosis Array Kit (R&D Systems, Minneapolis, USA) was used according to the manufacturer’s instructions. ..

Article Title: Erica spiculifolia Extract Potentiates Cisplatin Cytotoxicity by Reactivating p53 and Caspase-3-Dependent Apoptosis in Colorectal Carcinoma.
Article Snippet: .. Protein expression profiling was carried out using membrane-based sandwich immunoassays according to the manufacturer’s instructions (Human Apoptosis Array Kit, ARY009; R&D Systems, Minneapolis, MN, USA). .. Signal intensities of individual protein spots were quantified by densitometric analysis using ImageJ software (version 1.8.0) and subsequently converted into absolute protein expression values.

Article Title: Erica spiculifolia Extract Potentiates Cisplatin Cytotoxicity by Reactivating p53 and Caspase-3-Dependent Apoptosis in Colorectal Carcinoma
Article Snippet: .. Protein expression profiling was carried out using membrane-based sandwich immunoassays according to the manufacturer’s instructions (Human Apoptosis Array Kit, ARY009; R&D Systems, Minneapolis, MN, USA). .. Signal intensities of individual protein spots were quantified by densitometric analysis using ImageJ software (version 1.8.0) and subsequently converted into absolute protein expression values.

Article Title: Enhanced Payload Release Enables Disitamab Vedotin to Surpass Trastuzumab Emtansine and Retain Efficacy in Acquired Resistance to Clinical Anti-HER2 Therapies.
Article Snippet: After incubation, samples (50,000 cells) were acquired in an Accuri C6 Flow Cytometer (BD Biosciences), considering viable if they were negative for both Annexin V, which detects early apoptotic cells, and PI, which identifies necrotic cells. .. To analyze the expression profiles of apoptosis-related proteins, the Proteome Profiler Human Apoptosis Array Kit (R&D Systems, Minneapolis, USA) was used according to the manufacturer’s instructions. ..

Membrane:

Imaging:

Article Title: Functionalized nanoparticle transforms “cold” to “hot” adenoid cystic carcinoma of salivary gland tumour microenvironment in vitro
Article Snippet: .. Upon following manufacturer instruction of Proteome Profiler Human Apoptosis Array Kit (R&D Systems, Cat RDSARY009), high-resolution images were acquired using Chemidoc MP imaging system and processed using Image Lab version 6.0 (Bio-Rad Laboratories, Hercules, CA, USA). .. Quantification of relative protein expressions were done using microarray profile plugin in ImageJ (ImageJ 1.52a, National Institutes of Health, USA).

other:

Article Title: Marine trichodermamide B inhibits prostate cancer progression via catalase inhibition-induced apoptosis
Article Snippet: After treatment with either DMSO (0.1%, v/v) or TRH2 (8 μM) for 48 h, cells were harvested and proteins were extracted and analyzed according to the instructions of the Human Apoptosis Array Kit (Cat# ARY009, R&D Systems).

Article Title: Marine trichodermamide B inhibits prostate cancer progression via catalase inhibition-induced apoptosis.
Article Snippet: After treatment with either DMSO (0.1%, v/v) or TRH2 (8 μM) for 48 hours, cells were harvested and proteins were extracted and analyzed according to the instructions of the Human Apoptosis Array Kit (Cat# ARY009, R&D Systems).

Lysis:

Article Title: Functionalized nanoparticle transforms “cold” to “hot” adenoid cystic carcinoma of salivary gland tumour microenvironment in vitro
Article Snippet: .. The adenoid cystic carcinoma cells were added to 100 μL of lysis solution, supplied in Proteome Profiler Human Apoptosis Array Kit (R&D Systems, Cat RDSARY009) and 10 μL of protease inhibitor (Protease Inhibitor Cocktail I, Tocris, Cat 5500). ..

Protease Inhibitor: