human ace2 coding sequence (Addgene inc)

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Addgene inc human ace2 coding sequence
Human Ace2 Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 175 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+ace2+coding+sequence/hACE2+(Plasmid+%231786)/pm37661543-43-9-22
Average 96 stars, based on 175 article reviews

human ace2 coding sequence - by Bioz Stars, 2026-09

96/100 stars

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Construct:

Article Title: Targeting ACE2 with a camelid antibody inhibits SARS-CoV-2 binding and has protective effects in vivo
Article Snippet: Hamster ACE2 (haACE2) pcDNA3.1 hygro plasmid was synthetized by GeneArt (Thermo Fisher). .. The construct human ACE2 (hACE2) was obtained by substitution of the sequence encoding mCardinal from mCardinal-C1 plasmid (addgene, #54799) with hACE2 from the pLenti6-attB-hACE2-BSD . .. The construct myc-hACE2 was obtained by substitution of the sequence encoding mCardinal from mCardinal-C1 plasmid (addgene, #54799) with myc-hACE2 from the pCEP4-myc-ACE2 (addgene, #141185) .

Article Title: Targeting ACE2 with a camelid antibody inhibits SARS-CoV-2 binding and has protective effects in vivo.
Article Snippet: Hamster ACE2 (haACE2) pcDNA3.1 hygro plasmid was synthetized by GeneArt (Thermo Fisher). .. The construct human ACE2 (hACE2) was obtained by substitution of the sequence encoding mCardinal frommCardinal-C1 plasmid (addgene, #54799) with hACE2 from the pLenti6-attB-hACE2-BSD32. .. The construct myc-hACE2 was obtained by substitution of the sequence encoding mCardinal from mCardinal-C1 plasmid (addgene, #54799) with myc-hACE2 from the pCEP4-myc-ACE2 (addgene, #141185)59.

Sequencing:

Plasmid Preparation:

Article Title: Development and Application of a Pseudovirus-Based Assay for Modelling SARS-CoV-2 Spike Protein Mediated Drug Screening
Article Snippet: .. dHIV-VSV-G-hACE2 , pMD2.G (Addgene plasmid # 12259) , pCDH-EF1-FHC-hACE2. ..

Article Title: Small molecules that treat or prevent viral infections
Article Snippet: .. The plasmid expressing the human ACE2 gene was acquired from Addgene (Cat. No. 1786). .. The plasmid expressing the Delta Spike Protein (Cat. No. VG40819-UT) was acquired from Sino Biological.

Expressing:

Article Title: Pathogenicity, virological features, and immune evasion of SARS-CoV-2 JN.1-derived variants including JN.1.7, KP.2, KP.3, and KP.3.1.1
Article Snippet: A population of 293T cells was co-transfected with GFP1-10 (cat#68715, Addgene) along with SARS-CoV-2 spike expression plasmids to generate effector cells. .. Similarly, a separate set of 293T cells was co-transfected with GFP11 (cat#68716, Addgene) and human ACE2 expression plasmids to establish target cells. .. Twenty-four hours post-transfection, cells were harvested using EDTA-Trypsin (25200072, Gibco) and subsequently co-cultured at a 1:1 ratio in 8-well chamber slides for an additional 24 h. After incubation, cells were fixed with 10% formalin and permeabilized with 0.1% Triton X-100 (11332481001, Sigma, USA) at room temperature.

Article Title: Pathogenicity, virological features, and immune evasion of SARS-CoV-2 JN.1-derived variants including JN.1.7, KP.2, KP.3, and KP.3.1.1.
Article Snippet: Apopulation of 293T cellswas co-transfectedwithGFP1-10 (cat#68715, Addgene) along with SARS-CoV-2 spike expression plasmids to generate effector cells. .. Similarly, a separate set of 293T cells was cotransfected with GFP11 (cat#68716, Addgene) and human ACE2 expression plasmids to establish target cells. .. Twenty-four hours posttransfection, cells were harvested using EDTA-Trypsin (25200072, Gibco) and subsequently co-cultured at a 1:1 ratio in 8-well chamber slides for an additional 24 h. After incubation, cells were fixedwith 10% formalin and permeabilized with 0.1% Triton X-100 (11332481001, Sigma, USA) at room temperature.

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