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ace2  (Bioss)


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    Structured Review

    Bioss ace2
    a SARS-CoV-2 replication kinetics in HAE from different donors, HCoV-NL63 was used as a control ( n = 3). b Transepithelial electrical resistance (TEER in Ω cm 2 ) between the apical and basal poles was measured at each time point ( n = 3). c SARS-CoV-2 infected both ciliated cells (72 h pi) and secretory cells (72 h pi). arrows: virus particles, arrowhead: cilium, asterisk: secretory vesicle, insets dashed-line squares indicate magnification of arrowed areas. d Costaining of SARS-CoV-2 N protein (green) with ciliated cell marker β-tubulin-IV (red), goblet cell marker Muc5AC (red), club cell marker CCSP (red), and <t>ACE2</t> (red) positive cells. HCoV-NL63 N protein (green) staining was used as a control (72 h pi). Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (blue). Data a , b are the means ± s.d. of three independent biological replicates. Source data a – d are provided as a Source Data file.
    Ace2, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ace2+coding+sequence/ACE2+Polyclonal+Antibody/pmc07413383-1-0-2
    Average 94 stars, based on 31 article reviews
    ace2 - by Bioz Stars, 2026-11
    94/100 stars

    Images

    1) Product Images from "Morphogenesis and cytopathic effect of SARS-CoV-2 infection in human airway epithelial cells"

    Article Title: Morphogenesis and cytopathic effect of SARS-CoV-2 infection in human airway epithelial cells

    Journal: Nature Communications

    doi: 10.1038/s41467-020-17796-z

    a SARS-CoV-2 replication kinetics in HAE from different donors, HCoV-NL63 was used as a control ( n = 3). b Transepithelial electrical resistance (TEER in Ω cm 2 ) between the apical and basal poles was measured at each time point ( n = 3). c SARS-CoV-2 infected both ciliated cells (72 h pi) and secretory cells (72 h pi). arrows: virus particles, arrowhead: cilium, asterisk: secretory vesicle, insets dashed-line squares indicate magnification of arrowed areas. d Costaining of SARS-CoV-2 N protein (green) with ciliated cell marker β-tubulin-IV (red), goblet cell marker Muc5AC (red), club cell marker CCSP (red), and ACE2 (red) positive cells. HCoV-NL63 N protein (green) staining was used as a control (72 h pi). Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (blue). Data a , b are the means ± s.d. of three independent biological replicates. Source data a – d are provided as a Source Data file.
    Figure Legend Snippet: a SARS-CoV-2 replication kinetics in HAE from different donors, HCoV-NL63 was used as a control ( n = 3). b Transepithelial electrical resistance (TEER in Ω cm 2 ) between the apical and basal poles was measured at each time point ( n = 3). c SARS-CoV-2 infected both ciliated cells (72 h pi) and secretory cells (72 h pi). arrows: virus particles, arrowhead: cilium, asterisk: secretory vesicle, insets dashed-line squares indicate magnification of arrowed areas. d Costaining of SARS-CoV-2 N protein (green) with ciliated cell marker β-tubulin-IV (red), goblet cell marker Muc5AC (red), club cell marker CCSP (red), and ACE2 (red) positive cells. HCoV-NL63 N protein (green) staining was used as a control (72 h pi). Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (blue). Data a , b are the means ± s.d. of three independent biological replicates. Source data a – d are provided as a Source Data file.

    Techniques Used: Infection, Marker, Staining

    Source of antibodies and dyes with work concentration for immunofluorescence.
    Figure Legend Snippet: Source of antibodies and dyes with work concentration for immunofluorescence.

    Techniques Used: Concentration Assay, Immunofluorescence

    Related Articles

    Incubation:

    Article Title: Soybean Trypsin Inhibitor Possesses Potency Against SARS-CoV-2 Infection by Blocking the Host Cell Surface Receptors ACE2, TMPRSS2, and CD147.
    Article Snippet: After being dehydrated and cleared, the H&E staining results were visualized through microscopy (ECLIPSE TS100; Nikon, Tokyo, Japan) and photographed and recorded with a microscopic camera (ProgRes camera; Jenoptik AG, Jena, Germany). .. Sections of lungs, livers, and kidneys removed after sacrifice were incubated with ACE2 primary antibody (bs-1004R diluted 50×; Bioss, Woburn, MA, USA), TMPRSS2 primary antibody (ab214462 diluted 200×; Abcam, Cambridge, UK), or CD147 primary antibody (R381696 diluted 50×; Zen-BioScience, Chengdu, China), respectively. .. IHC evaluation was performed using the Polink-2 Plus HRP Rabbit DAB Detection Kit (D39; OriGene, Rockville, MD, USA) according to the recommended protocol from the manufacturer, and microscopic examination of the results was conducted using a microscope (ECLIPSE TS100; Nikon, Japan), with images captured with a microscope camera (ProgRes camera; Jenoptik AG, Germany).

    Article Title: Soybean Trypsin Inhibitor Possesses Potency Against SARS-CoV-2 Infection by Blocking the Host Cell Surface Receptors ACE2, TMPRSS2, and CD147
    Article Snippet: After being dehydrated and cleared, the H&E staining results were visualized through microscopy (ECLIPSE TS100; Nikon, Tokyo, Japan) and photographed and recorded with a microscopic camera (ProgRes camera; Jenoptik AG, Jena, Germany). .. Sections of lungs, livers, and kidneys removed after sacrifice were incubated with ACE2 primary antibody (bs-1004R diluted 50×; Bioss, Woburn, MA, USA), TMPRSS2 primary antibody (ab214462 diluted 200×; Abcam, Cambridge, UK), or CD147 primary antibody (R381696 diluted 50×; Zen-BioScience, Chengdu, China), respectively. .. IHC evaluation was performed using the Polink-2 Plus HRP Rabbit DAB Detection Kit (D39; OriGene, Rockville, MD, USA) according to the recommended protocol from the manufacturer, and microscopic examination of the results was conducted using a microscope (ECLIPSE TS100; Nikon, Japan), with images captured with a microscope camera (ProgRes camera; Jenoptik AG, Germany).

    Immunohistochemical staining:

    Article Title: Bioactive Compounds of Momordica charantia L. Downregulate the Protein Expression of ACE2 and TMPRSS2 In Vivo and In Vitro.
    Article Snippet: .. Immunohistochemical staining was performed by incubating the sections with primary antibodies targeting angiotensin-converting enzyme 2 (ACE2; bs-1004R, Bioss Inc., Woburn, https://doi.org/10.3390/ijms27020868 MA, USA; 1:50 dilution) and transmembrane serine protease 2 (TMPRSS2; ab214462, Abcam, Cambridge, UK; 1:200 dilution) overnight at 4 ◦C, following antigen retrieval and blocking of non-specific binding sites. .. Visualization and image acquisition were conducted using a Nikon ECLIPSE TS100 light microscope (Nikon Corporation, Tokyo, Japan) equipped with a digital imaging system.

    Article Title: Bioactive Compounds of Momordica charantia L. Downregulate the Protein Expression of ACE2 and TMPRSS2 In Vivo and In Vitro
    Article Snippet: .. Immunohistochemical staining was performed by incubating the sections with primary antibodies targeting angiotensin-converting enzyme 2 (ACE2; bs-1004R, Bioss Inc., Woburn, MA, USA; 1:50 dilution) and transmembrane serine protease 2 (TMPRSS2; ab214462, Abcam, Cambridge, UK; 1:200 dilution) overnight at 4 °C, following antigen retrieval and blocking of non-specific binding sites. .. Visualization and image acquisition were conducted using a Nikon ECLIPSE TS100 light microscope (Nikon Corporation, Tokyo, Japan) equipped with a digital imaging system.

    Staining:

    Article Title: Bioactive Compounds of Momordica charantia L. Downregulate the Protein Expression of ACE2 and TMPRSS2 In Vivo and In Vitro.
    Article Snippet: .. Immunohistochemical staining was performed by incubating the sections with primary antibodies targeting angiotensin-converting enzyme 2 (ACE2; bs-1004R, Bioss Inc., Woburn, https://doi.org/10.3390/ijms27020868 MA, USA; 1:50 dilution) and transmembrane serine protease 2 (TMPRSS2; ab214462, Abcam, Cambridge, UK; 1:200 dilution) overnight at 4 ◦C, following antigen retrieval and blocking of non-specific binding sites. .. Visualization and image acquisition were conducted using a Nikon ECLIPSE TS100 light microscope (Nikon Corporation, Tokyo, Japan) equipped with a digital imaging system.

    Article Title: Immediate myeloid depot for SARS-CoV-2 in the human lung
    Article Snippet: .. After infection, PCLS were fixed in 4% PFA for at least 30 min. After saturation in PBS with 1% bovine serum albumin (BSA), slices were stained with anti-CD45 (HI30, 304056, BioLegend), anti-spike (40150-R007, Sino Biological), anti-ACE2 (bs-1004R, BIOSS) anti-dsRNA (J2, 10010, Scicons), or anti–EpCAM-AF488 (9C4, 324210, BioLegend) for 1 hour in PBS with 1% BSA media at room temperature. .. After infection, PCLS were fixed in 4% PFA for at least 30 min. After saturation in PBS with 1% bovine serum albumin (BSA), slices were stained with anti-CD45 (HI30, 304056, BioLegend), anti-spike (40150-R007, Sino Biological), anti-ACE2 (bs-1004R, BIOSS) anti-dsRNA (J2, 10010, Scicons), or anti–EpCAM-AF488 (9C4, 324210, BioLegend) for 1 hour in PBS with 1% BSA media at room temperature.

    Article Title: Immediate myeloid depot for SARS-CoV-2 in the human lung
    Article Snippet: .. Staining Antibody (clone) Lot number Dye Catalog number (Supplier) Surface Staining CD169 1621398 AF594 FAB5197T (R&D Systems) ACE2 BJ07067522 PE bs-1004R (BIOSS) EpCAM B250368 BV650 324226 (BioLegend) CD31 (WM59) 8232937 BV605 562855 (BD Biosciences) CD45 (HI30) B333796 BV421 304032 (BioLegend) CD14 (M5E2) B275828 BV711 301838 (BioLegend) CD3 (SK7) 0314461 BB700 566575 (BD Biosciences) CD19 (SJ25C1) 1069967 BB700 566396 (BD Biosciences) Zombie (viability) B331984 NIR 77184 (BioLegend) HLA-DR (G46-6) 1266420 BUV395 564040 (BD Biosciences) Intracellular Staining Spike 1619059 AF647 FAB105805R (R&D Systems) dsRNA (J2) J2-2007 AF488 10010 (Scicons) Table S5. ..

    Article Title: Bioactive Compounds of Momordica charantia L. Downregulate the Protein Expression of ACE2 and TMPRSS2 In Vivo and In Vitro
    Article Snippet: .. Immunohistochemical staining was performed by incubating the sections with primary antibodies targeting angiotensin-converting enzyme 2 (ACE2; bs-1004R, Bioss Inc., Woburn, MA, USA; 1:50 dilution) and transmembrane serine protease 2 (TMPRSS2; ab214462, Abcam, Cambridge, UK; 1:200 dilution) overnight at 4 °C, following antigen retrieval and blocking of non-specific binding sites. .. Visualization and image acquisition were conducted using a Nikon ECLIPSE TS100 light microscope (Nikon Corporation, Tokyo, Japan) equipped with a digital imaging system.

    Blocking Assay:

    Article Title: Bioactive Compounds of Momordica charantia L. Downregulate the Protein Expression of ACE2 and TMPRSS2 In Vivo and In Vitro.
    Article Snippet: .. Immunohistochemical staining was performed by incubating the sections with primary antibodies targeting angiotensin-converting enzyme 2 (ACE2; bs-1004R, Bioss Inc., Woburn, https://doi.org/10.3390/ijms27020868 MA, USA; 1:50 dilution) and transmembrane serine protease 2 (TMPRSS2; ab214462, Abcam, Cambridge, UK; 1:200 dilution) overnight at 4 ◦C, following antigen retrieval and blocking of non-specific binding sites. .. Visualization and image acquisition were conducted using a Nikon ECLIPSE TS100 light microscope (Nikon Corporation, Tokyo, Japan) equipped with a digital imaging system.

    Article Title: Bioactive Compounds of Momordica charantia L. Downregulate the Protein Expression of ACE2 and TMPRSS2 In Vivo and In Vitro
    Article Snippet: .. Immunohistochemical staining was performed by incubating the sections with primary antibodies targeting angiotensin-converting enzyme 2 (ACE2; bs-1004R, Bioss Inc., Woburn, MA, USA; 1:50 dilution) and transmembrane serine protease 2 (TMPRSS2; ab214462, Abcam, Cambridge, UK; 1:200 dilution) overnight at 4 °C, following antigen retrieval and blocking of non-specific binding sites. .. Visualization and image acquisition were conducted using a Nikon ECLIPSE TS100 light microscope (Nikon Corporation, Tokyo, Japan) equipped with a digital imaging system.

    Binding Assay:

    Article Title: Bioactive Compounds of Momordica charantia L. Downregulate the Protein Expression of ACE2 and TMPRSS2 In Vivo and In Vitro.
    Article Snippet: .. Immunohistochemical staining was performed by incubating the sections with primary antibodies targeting angiotensin-converting enzyme 2 (ACE2; bs-1004R, Bioss Inc., Woburn, https://doi.org/10.3390/ijms27020868 MA, USA; 1:50 dilution) and transmembrane serine protease 2 (TMPRSS2; ab214462, Abcam, Cambridge, UK; 1:200 dilution) overnight at 4 ◦C, following antigen retrieval and blocking of non-specific binding sites. .. Visualization and image acquisition were conducted using a Nikon ECLIPSE TS100 light microscope (Nikon Corporation, Tokyo, Japan) equipped with a digital imaging system.

    Article Title: Bioactive Compounds of Momordica charantia L. Downregulate the Protein Expression of ACE2 and TMPRSS2 In Vivo and In Vitro
    Article Snippet: .. Immunohistochemical staining was performed by incubating the sections with primary antibodies targeting angiotensin-converting enzyme 2 (ACE2; bs-1004R, Bioss Inc., Woburn, MA, USA; 1:50 dilution) and transmembrane serine protease 2 (TMPRSS2; ab214462, Abcam, Cambridge, UK; 1:200 dilution) overnight at 4 °C, following antigen retrieval and blocking of non-specific binding sites. .. Visualization and image acquisition were conducted using a Nikon ECLIPSE TS100 light microscope (Nikon Corporation, Tokyo, Japan) equipped with a digital imaging system.

    Infection:

    Article Title: Immediate myeloid depot for SARS-CoV-2 in the human lung
    Article Snippet: .. After infection, PCLS were fixed in 4% PFA for at least 30 min. After saturation in PBS with 1% bovine serum albumin (BSA), slices were stained with anti-CD45 (HI30, 304056, BioLegend), anti-spike (40150-R007, Sino Biological), anti-ACE2 (bs-1004R, BIOSS) anti-dsRNA (J2, 10010, Scicons), or anti–EpCAM-AF488 (9C4, 324210, BioLegend) for 1 hour in PBS with 1% BSA media at room temperature. .. After infection, PCLS were fixed in 4% PFA for at least 30 min. After saturation in PBS with 1% bovine serum albumin (BSA), slices were stained with anti-CD45 (HI30, 304056, BioLegend), anti-spike (40150-R007, Sino Biological), anti-ACE2 (bs-1004R, BIOSS) anti-dsRNA (J2, 10010, Scicons), or anti–EpCAM-AF488 (9C4, 324210, BioLegend) for 1 hour in PBS with 1% BSA media at room temperature.



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