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ht29 ahr reporter assay ht29 lucia ahr cells  (InvivoGen)


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    InvivoGen ht29 ahr reporter assay ht29 lucia ahr cells
    Ht29 Ahr Reporter Assay Ht29 Lucia Ahr Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ht29/HT29-Lucia+AhR+Cells/us12371408-811-3-13
    Average 95 stars, based on 59 article reviews
    ht29 ahr reporter assay ht29 lucia ahr cells - by Bioz Stars, 2026-09
    95/100 stars

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    Recombinant:

    Article Title: Genomic and phenotypic comparisons reveal lineage-specific traits of the probiotic Saccharomyces boulardii .
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples S. boulardii CNCM I-745 Biocodex (Precosa) CNCM I-745 S. boulardii DBVPG 6763 Gnosis (Sachhaflor) DBVPG 6763 S. boulardii ATCC MYA-796 ATCC MYA-796 S. boulardii CNCM I-3799 NOW Foods CNCM I-3799 S. boulardii CNCM I-1079 Lallemand CNCM I-1079 S. cerevisiae baker’s (SE) KronJäst N/A S. cerevisiae baker’s (DK) Lallemand N/A S. cerevisiae CEN.PK113-5D Euroscarf CEN.PK113-5D Chemicals, peptides, and recombinant proteins DNA/RNA Shield Zymo Research Cat. No. R1100 Artificial gastric fluid Biochemazone Cat. No. BZ175 Artificial intestinal fluid Biochemazone Cat. No. BZ176 Artificial colonic fluid Biochemazone Cat. No. BZ177 Yeast Live/Dead kit Biotium Cat. No. 31064 D-(+)-Glucose monohydrate Sigma-Aldrich Cat. No. 16301 Ethanol absolute VWR Cat. No. 20821.310 Sodium acetate Sigma-Aldrich Cat. No. S8750 Sodium L-lactate Sigma-Aldrich Cat. No. 71718 Sodium succinate dibasic Sigma-Aldrich Cat. No. 14160 McCoy’s 5A medium Thermo Fisher Cat. No. 16600082 Fetal bovine serum Thermo Fisher Cat. No. A5670701 Penicillin–streptomycin Thermo Fisher Cat. No. 15140148 RPMI-1640 medium Thermo Fisher Cat. No. 12027599 PMA Sigma-Aldrich Cat. No. P8139 Zeocin InvivoGen Cat. No. 11006-33-0 Puromycin Thermo Fisher Cat. No. A1113803 2-Mercaptoethanol Thermo Fisher Cat. No. 21985023 LPS (E. coli O111:B4) Sigma-Aldrich Cat. No. L2630 TNFα R&D Systems Cat. No. 10291-TA FICZ Sigma-Aldrich Cat. No. 172922-91-7 Critical commercial assays QUANTI-Luc reagent InvivoGen Cat. No. rep-qlc4r2 Bright-Glo Luciferase assay Promega Cat. No. E2610 IL-8 ELISA kit R&D Systems Cat. No. DY208 TNFα ELISA kit Abcam Cat. No. ab181421 Yeast Live-or-DyeTM Live/Dead kit Biotium Cat. No. 31064 Deposited data PRJNA1312645 whole genome sequencing dataset NCBI https://www.ncbi.nlm.nih.gov/sra/PRJNA1312645 Experimental models: Cell lines HT-29 cells ATCC Cat. No. HTB-38 HT29-Lucia AhR cells InvivoGen Cat. No. ht2l-ahr THP-1 cells ATCC Cat. No. TIB-202 THP-1 NF-κB reporter cells ATCC Cat. No. TIB-202-NFkB-LUC2 Caco-2 cells ATCC Cat. No. HTB-37 (Continued on next page) e1 iScience 29, 115706, May 15, 2026 ..

    Luciferase:

    Article Title: Genomic and phenotypic comparisons reveal lineage-specific traits of the probiotic Saccharomyces boulardii .
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples S. boulardii CNCM I-745 Biocodex (Precosa) CNCM I-745 S. boulardii DBVPG 6763 Gnosis (Sachhaflor) DBVPG 6763 S. boulardii ATCC MYA-796 ATCC MYA-796 S. boulardii CNCM I-3799 NOW Foods CNCM I-3799 S. boulardii CNCM I-1079 Lallemand CNCM I-1079 S. cerevisiae baker’s (SE) KronJäst N/A S. cerevisiae baker’s (DK) Lallemand N/A S. cerevisiae CEN.PK113-5D Euroscarf CEN.PK113-5D Chemicals, peptides, and recombinant proteins DNA/RNA Shield Zymo Research Cat. No. R1100 Artificial gastric fluid Biochemazone Cat. No. BZ175 Artificial intestinal fluid Biochemazone Cat. No. BZ176 Artificial colonic fluid Biochemazone Cat. No. BZ177 Yeast Live/Dead kit Biotium Cat. No. 31064 D-(+)-Glucose monohydrate Sigma-Aldrich Cat. No. 16301 Ethanol absolute VWR Cat. No. 20821.310 Sodium acetate Sigma-Aldrich Cat. No. S8750 Sodium L-lactate Sigma-Aldrich Cat. No. 71718 Sodium succinate dibasic Sigma-Aldrich Cat. No. 14160 McCoy’s 5A medium Thermo Fisher Cat. No. 16600082 Fetal bovine serum Thermo Fisher Cat. No. A5670701 Penicillin–streptomycin Thermo Fisher Cat. No. 15140148 RPMI-1640 medium Thermo Fisher Cat. No. 12027599 PMA Sigma-Aldrich Cat. No. P8139 Zeocin InvivoGen Cat. No. 11006-33-0 Puromycin Thermo Fisher Cat. No. A1113803 2-Mercaptoethanol Thermo Fisher Cat. No. 21985023 LPS (E. coli O111:B4) Sigma-Aldrich Cat. No. L2630 TNFα R&D Systems Cat. No. 10291-TA FICZ Sigma-Aldrich Cat. No. 172922-91-7 Critical commercial assays QUANTI-Luc reagent InvivoGen Cat. No. rep-qlc4r2 Bright-Glo Luciferase assay Promega Cat. No. E2610 IL-8 ELISA kit R&D Systems Cat. No. DY208 TNFα ELISA kit Abcam Cat. No. ab181421 Yeast Live-or-DyeTM Live/Dead kit Biotium Cat. No. 31064 Deposited data PRJNA1312645 whole genome sequencing dataset NCBI https://www.ncbi.nlm.nih.gov/sra/PRJNA1312645 Experimental models: Cell lines HT-29 cells ATCC Cat. No. HTB-38 HT29-Lucia AhR cells InvivoGen Cat. No. ht2l-ahr THP-1 cells ATCC Cat. No. TIB-202 THP-1 NF-κB reporter cells ATCC Cat. No. TIB-202-NFkB-LUC2 Caco-2 cells ATCC Cat. No. HTB-37 (Continued on next page) e1 iScience 29, 115706, May 15, 2026 ..

    Article Title: Kidney disease reprograms microbiome-host signaling to promote heart failure
    Article Snippet: .. Human AhR reporter cell line (HT29-Lucia TM AhR, InvivoGen, ht21-ahr), containing AhR-responsive DRE upstream of a Lucia luciferase gene, was cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% FBS and 100 μg/mL Zeocin (InvivoGen, ant-zn-05), maintained at 37 °C with 5% CO 2 . ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Genomic and phenotypic comparisons reveal lineage-specific traits of the probiotic Saccharomyces boulardii .
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples S. boulardii CNCM I-745 Biocodex (Precosa) CNCM I-745 S. boulardii DBVPG 6763 Gnosis (Sachhaflor) DBVPG 6763 S. boulardii ATCC MYA-796 ATCC MYA-796 S. boulardii CNCM I-3799 NOW Foods CNCM I-3799 S. boulardii CNCM I-1079 Lallemand CNCM I-1079 S. cerevisiae baker’s (SE) KronJäst N/A S. cerevisiae baker’s (DK) Lallemand N/A S. cerevisiae CEN.PK113-5D Euroscarf CEN.PK113-5D Chemicals, peptides, and recombinant proteins DNA/RNA Shield Zymo Research Cat. No. R1100 Artificial gastric fluid Biochemazone Cat. No. BZ175 Artificial intestinal fluid Biochemazone Cat. No. BZ176 Artificial colonic fluid Biochemazone Cat. No. BZ177 Yeast Live/Dead kit Biotium Cat. No. 31064 D-(+)-Glucose monohydrate Sigma-Aldrich Cat. No. 16301 Ethanol absolute VWR Cat. No. 20821.310 Sodium acetate Sigma-Aldrich Cat. No. S8750 Sodium L-lactate Sigma-Aldrich Cat. No. 71718 Sodium succinate dibasic Sigma-Aldrich Cat. No. 14160 McCoy’s 5A medium Thermo Fisher Cat. No. 16600082 Fetal bovine serum Thermo Fisher Cat. No. A5670701 Penicillin–streptomycin Thermo Fisher Cat. No. 15140148 RPMI-1640 medium Thermo Fisher Cat. No. 12027599 PMA Sigma-Aldrich Cat. No. P8139 Zeocin InvivoGen Cat. No. 11006-33-0 Puromycin Thermo Fisher Cat. No. A1113803 2-Mercaptoethanol Thermo Fisher Cat. No. 21985023 LPS (E. coli O111:B4) Sigma-Aldrich Cat. No. L2630 TNFα R&D Systems Cat. No. 10291-TA FICZ Sigma-Aldrich Cat. No. 172922-91-7 Critical commercial assays QUANTI-Luc reagent InvivoGen Cat. No. rep-qlc4r2 Bright-Glo Luciferase assay Promega Cat. No. E2610 IL-8 ELISA kit R&D Systems Cat. No. DY208 TNFα ELISA kit Abcam Cat. No. ab181421 Yeast Live-or-DyeTM Live/Dead kit Biotium Cat. No. 31064 Deposited data PRJNA1312645 whole genome sequencing dataset NCBI https://www.ncbi.nlm.nih.gov/sra/PRJNA1312645 Experimental models: Cell lines HT-29 cells ATCC Cat. No. HTB-38 HT29-Lucia AhR cells InvivoGen Cat. No. ht2l-ahr THP-1 cells ATCC Cat. No. TIB-202 THP-1 NF-κB reporter cells ATCC Cat. No. TIB-202-NFkB-LUC2 Caco-2 cells ATCC Cat. No. HTB-37 (Continued on next page) e1 iScience 29, 115706, May 15, 2026 ..

    Sequencing:

    Article Title: Genomic and phenotypic comparisons reveal lineage-specific traits of the probiotic Saccharomyces boulardii .
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples S. boulardii CNCM I-745 Biocodex (Precosa) CNCM I-745 S. boulardii DBVPG 6763 Gnosis (Sachhaflor) DBVPG 6763 S. boulardii ATCC MYA-796 ATCC MYA-796 S. boulardii CNCM I-3799 NOW Foods CNCM I-3799 S. boulardii CNCM I-1079 Lallemand CNCM I-1079 S. cerevisiae baker’s (SE) KronJäst N/A S. cerevisiae baker’s (DK) Lallemand N/A S. cerevisiae CEN.PK113-5D Euroscarf CEN.PK113-5D Chemicals, peptides, and recombinant proteins DNA/RNA Shield Zymo Research Cat. No. R1100 Artificial gastric fluid Biochemazone Cat. No. BZ175 Artificial intestinal fluid Biochemazone Cat. No. BZ176 Artificial colonic fluid Biochemazone Cat. No. BZ177 Yeast Live/Dead kit Biotium Cat. No. 31064 D-(+)-Glucose monohydrate Sigma-Aldrich Cat. No. 16301 Ethanol absolute VWR Cat. No. 20821.310 Sodium acetate Sigma-Aldrich Cat. No. S8750 Sodium L-lactate Sigma-Aldrich Cat. No. 71718 Sodium succinate dibasic Sigma-Aldrich Cat. No. 14160 McCoy’s 5A medium Thermo Fisher Cat. No. 16600082 Fetal bovine serum Thermo Fisher Cat. No. A5670701 Penicillin–streptomycin Thermo Fisher Cat. No. 15140148 RPMI-1640 medium Thermo Fisher Cat. No. 12027599 PMA Sigma-Aldrich Cat. No. P8139 Zeocin InvivoGen Cat. No. 11006-33-0 Puromycin Thermo Fisher Cat. No. A1113803 2-Mercaptoethanol Thermo Fisher Cat. No. 21985023 LPS (E. coli O111:B4) Sigma-Aldrich Cat. No. L2630 TNFα R&D Systems Cat. No. 10291-TA FICZ Sigma-Aldrich Cat. No. 172922-91-7 Critical commercial assays QUANTI-Luc reagent InvivoGen Cat. No. rep-qlc4r2 Bright-Glo Luciferase assay Promega Cat. No. E2610 IL-8 ELISA kit R&D Systems Cat. No. DY208 TNFα ELISA kit Abcam Cat. No. ab181421 Yeast Live-or-DyeTM Live/Dead kit Biotium Cat. No. 31064 Deposited data PRJNA1312645 whole genome sequencing dataset NCBI https://www.ncbi.nlm.nih.gov/sra/PRJNA1312645 Experimental models: Cell lines HT-29 cells ATCC Cat. No. HTB-38 HT29-Lucia AhR cells InvivoGen Cat. No. ht2l-ahr THP-1 cells ATCC Cat. No. TIB-202 THP-1 NF-κB reporter cells ATCC Cat. No. TIB-202-NFkB-LUC2 Caco-2 cells ATCC Cat. No. HTB-37 (Continued on next page) e1 iScience 29, 115706, May 15, 2026 ..

    Activation Assay:

    Article Title: Commercial milk kefir exerts immunomodulatory TLR-mediated responses on in vitro models of the human intestinal epithelium.
    Article Snippet: .. Commercial cell lines HT29-Lucia AhR, HEK-Blue hTLR4 and HEKBlue-Lucia hTLR9 (NF/IL8) (InvivoGen Inc) were used to evaluate activation of the corresponding signaling pathways. ..

    Protein-Protein interactions:

    Article Title: Commercial milk kefir exerts immunomodulatory TLR-mediated responses on in vitro models of the human intestinal epithelium.
    Article Snippet: .. Commercial cell lines HT29-Lucia AhR, HEK-Blue hTLR4 and HEKBlue-Lucia hTLR9 (NF/IL8) (InvivoGen Inc) were used to evaluate activation of the corresponding signaling pathways. ..

    Modification:

    Article Title: CU104, a novel barrier function enhancer, improves colitis via modulation of barrier function and immune cell recruitment
    Article Snippet: .. Caco-2, RAW264.7 (ATCC, Manassas, VA, USA), HCT-116-DualTM, and HT29-LuciaTM AhR cells (InvivoGen, San Diego, CA, USA) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; HyClone, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. .. HL-60 cells (ATCC) were cultured in RPMI 1640 medium containing 20% FBS and 1% penicillin-streptomycin.

    Article Title: Kidney disease reprograms microbiome-host signaling to promote heart failure
    Article Snippet: .. Human AhR reporter cell line (HT29-Lucia TM AhR, InvivoGen, ht21-ahr), containing AhR-responsive DRE upstream of a Lucia luciferase gene, was cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% FBS and 100 μg/mL Zeocin (InvivoGen, ant-zn-05), maintained at 37 °C with 5% CO 2 . ..

    Cell Culture:

    Article Title: Kidney disease reprograms microbiome-host signaling to promote heart failure
    Article Snippet: .. Human AhR reporter cell line (HT29-Lucia TM AhR, InvivoGen, ht21-ahr), containing AhR-responsive DRE upstream of a Lucia luciferase gene, was cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% FBS and 100 μg/mL Zeocin (InvivoGen, ant-zn-05), maintained at 37 °C with 5% CO 2 . ..



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    ATCC lung lung carcinoma nsclc ht29 htb
    oHSV-1 exhibits the characteristics of a standard one-step growth curve and can efficiently replicate and express exogenous genes (A) The results of virus titer determination of HSV-1/WT, RG2001m, RG2002m, RG2005m, RG2011m, and RG2012m in Vero cells 48 h after infection. (B) Vero cells were infected with oHSV-1 at a multiplicity of infection (MOI) = 1, and the virus titers were determined at 0, 8, 16, 24, 32, 40, and 48 h, respectively, and a growth curve was drawn. (C) The results of virus titer determination of oHSV-1 in colon cancer cells (CT26, HCT116, and <t>HT29)</t> 48 h after infection. (D–F) The growth curves of oHSV-1 in colon cancer cells (CT26, HCT116, and HT29). (G) At MOI = 10, IL-15 expression levels in cell supernatants of HSV-1/WT, RG2001m, RG2005m, and RG2012m at 24, 48, and 72 h post-infection (mouse IL-15 ELISA Kit, elabscience). (H) At MOI = 10, IL-12 expression levels in cell supernatants of HSV-1/WT, RG2002m, RG2005m, and RG2012m at 24, 48, and 72 h post-infection (mouse IL-12 ELISA Kit, elabscience). (I) At MOI = 10, chemokine CCL5 expression levels in cell supernatants of HSV-1/WT, RG2011m, and RG2012m at 24, 48, and 72 h post-infection (mouse CCL5 ELISA Kit, elabscience). (Data are presented as mean ± SD. Comparisons between datasets were performed using one-way ANOVA and Student’s t test, with p values calculated [ n = 3]) ( p > 0.05, ns; p < 0.05, ∗; p < 0.01, ∗ ∗; p < 0.001, ∗ ∗ ∗).
    Lung Lung Carcinoma Nsclc Ht29 Htb, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ht29 colorectal carcinoma cells  (ATCC)
    99
    ATCC ht29 colorectal carcinoma cells
    oHSV-1 exhibits the characteristics of a standard one-step growth curve and can efficiently replicate and express exogenous genes (A) The results of virus titer determination of HSV-1/WT, RG2001m, RG2002m, RG2005m, RG2011m, and RG2012m in Vero cells 48 h after infection. (B) Vero cells were infected with oHSV-1 at a multiplicity of infection (MOI) = 1, and the virus titers were determined at 0, 8, 16, 24, 32, 40, and 48 h, respectively, and a growth curve was drawn. (C) The results of virus titer determination of oHSV-1 in colon cancer cells (CT26, HCT116, and <t>HT29)</t> 48 h after infection. (D–F) The growth curves of oHSV-1 in colon cancer cells (CT26, HCT116, and HT29). (G) At MOI = 10, IL-15 expression levels in cell supernatants of HSV-1/WT, RG2001m, RG2005m, and RG2012m at 24, 48, and 72 h post-infection (mouse IL-15 ELISA Kit, elabscience). (H) At MOI = 10, IL-12 expression levels in cell supernatants of HSV-1/WT, RG2002m, RG2005m, and RG2012m at 24, 48, and 72 h post-infection (mouse IL-12 ELISA Kit, elabscience). (I) At MOI = 10, chemokine CCL5 expression levels in cell supernatants of HSV-1/WT, RG2011m, and RG2012m at 24, 48, and 72 h post-infection (mouse CCL5 ELISA Kit, elabscience). (Data are presented as mean ± SD. Comparisons between datasets were performed using one-way ANOVA and Student’s t test, with p values calculated [ n = 3]) ( p > 0.05, ns; p < 0.05, ∗; p < 0.01, ∗ ∗; p < 0.001, ∗ ∗ ∗).
    Ht29 Colorectal Carcinoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ht29 colorectal carcinoma cells - by Bioz Stars, 2026-09
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    cells ht29  (ATCC)
    99
    ATCC cells ht29
    oHSV-1 exhibits the characteristics of a standard one-step growth curve and can efficiently replicate and express exogenous genes (A) The results of virus titer determination of HSV-1/WT, RG2001m, RG2002m, RG2005m, RG2011m, and RG2012m in Vero cells 48 h after infection. (B) Vero cells were infected with oHSV-1 at a multiplicity of infection (MOI) = 1, and the virus titers were determined at 0, 8, 16, 24, 32, 40, and 48 h, respectively, and a growth curve was drawn. (C) The results of virus titer determination of oHSV-1 in colon cancer cells (CT26, HCT116, and <t>HT29)</t> 48 h after infection. (D–F) The growth curves of oHSV-1 in colon cancer cells (CT26, HCT116, and HT29). (G) At MOI = 10, IL-15 expression levels in cell supernatants of HSV-1/WT, RG2001m, RG2005m, and RG2012m at 24, 48, and 72 h post-infection (mouse IL-15 ELISA Kit, elabscience). (H) At MOI = 10, IL-12 expression levels in cell supernatants of HSV-1/WT, RG2002m, RG2005m, and RG2012m at 24, 48, and 72 h post-infection (mouse IL-12 ELISA Kit, elabscience). (I) At MOI = 10, chemokine CCL5 expression levels in cell supernatants of HSV-1/WT, RG2011m, and RG2012m at 24, 48, and 72 h post-infection (mouse CCL5 ELISA Kit, elabscience). (Data are presented as mean ± SD. Comparisons between datasets were performed using one-way ANOVA and Student’s t test, with p values calculated [ n = 3]) ( p > 0.05, ns; p < 0.05, ∗; p < 0.01, ∗ ∗; p < 0.001, ∗ ∗ ∗).
    Cells Ht29, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ht29 lung metastatic colonization model tissue sections  (Nature Biotechnology)
    86
    Nature Biotechnology ht29 lung metastatic colonization model tissue sections
    oHSV-1 exhibits the characteristics of a standard one-step growth curve and can efficiently replicate and express exogenous genes (A) The results of virus titer determination of HSV-1/WT, RG2001m, RG2002m, RG2005m, RG2011m, and RG2012m in Vero cells 48 h after infection. (B) Vero cells were infected with oHSV-1 at a multiplicity of infection (MOI) = 1, and the virus titers were determined at 0, 8, 16, 24, 32, 40, and 48 h, respectively, and a growth curve was drawn. (C) The results of virus titer determination of oHSV-1 in colon cancer cells (CT26, HCT116, and <t>HT29)</t> 48 h after infection. (D–F) The growth curves of oHSV-1 in colon cancer cells (CT26, HCT116, and HT29). (G) At MOI = 10, IL-15 expression levels in cell supernatants of HSV-1/WT, RG2001m, RG2005m, and RG2012m at 24, 48, and 72 h post-infection (mouse IL-15 ELISA Kit, elabscience). (H) At MOI = 10, IL-12 expression levels in cell supernatants of HSV-1/WT, RG2002m, RG2005m, and RG2012m at 24, 48, and 72 h post-infection (mouse IL-12 ELISA Kit, elabscience). (I) At MOI = 10, chemokine CCL5 expression levels in cell supernatants of HSV-1/WT, RG2011m, and RG2012m at 24, 48, and 72 h post-infection (mouse CCL5 ELISA Kit, elabscience). (Data are presented as mean ± SD. Comparisons between datasets were performed using one-way ANOVA and Student’s t test, with p values calculated [ n = 3]) ( p > 0.05, ns; p < 0.05, ∗; p < 0.01, ∗ ∗; p < 0.001, ∗ ∗ ∗).
    Ht29 Lung Metastatic Colonization Model Tissue Sections, supplied by Nature Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Biochemical structures of Linebacker-1 (LB1) and myricetin and antineoplastic effect of LB1 with/without radiation in human colon and lung cell lines. (A) Myricetin (left) and LB1 (right) showing with a red circle for the OH ion’s replacement by a cloride ion (Cl). (B) Schematic diagram of the treatment design using LB1 for in vitro study. (C) and (D) MTT growth assay using OD value 570 nm, showing the antitumor effect of LB1 in titrated doses for both human colon (HT29) and lung (A549) cancer cell lines, respectively. (E) Bar graph representing the colony count numbers from the clonogenic survival assay after LB1 treatment. (F) Schematic diagram showing the treatment design for in vitro LB1 and radiation treatment. Doses of 0, 25, 50, and 75 µM LB1, along with single radiation fractions of 0, 2, 4, and 6 Gy, were used in the clonogenic survival study. (G) For the clonogenic survival assay, colony counts are shown for A549 cells treated with 0, 2, 4, and 6 Gy of radiation, with or without LB1, at 0, 25, 50, and 75 µM. (H) colony count for A549 and Lewis lung carcinoma (LLC1) cell line , respectively. Graphs are generated from the average of at least 3 study repeats. The SD of the multiple independent repeats is shown, while statistical significance is shown by *** P < .001. Abbreviation : OD = optical density; OH = hydroxide ion; MTT = methyl dimethylthiazol diphenyltetrazolium bromide; RT = radiation therapy.

    Journal: Advances in Radiation Oncology

    Article Title: Synergistic Effect of Linebacker-1 With Radiation Therapy in a Mouse Lung Cancer Model

    doi: 10.1016/j.adro.2026.102051

    Figure Lengend Snippet: Biochemical structures of Linebacker-1 (LB1) and myricetin and antineoplastic effect of LB1 with/without radiation in human colon and lung cell lines. (A) Myricetin (left) and LB1 (right) showing with a red circle for the OH ion’s replacement by a cloride ion (Cl). (B) Schematic diagram of the treatment design using LB1 for in vitro study. (C) and (D) MTT growth assay using OD value 570 nm, showing the antitumor effect of LB1 in titrated doses for both human colon (HT29) and lung (A549) cancer cell lines, respectively. (E) Bar graph representing the colony count numbers from the clonogenic survival assay after LB1 treatment. (F) Schematic diagram showing the treatment design for in vitro LB1 and radiation treatment. Doses of 0, 25, 50, and 75 µM LB1, along with single radiation fractions of 0, 2, 4, and 6 Gy, were used in the clonogenic survival study. (G) For the clonogenic survival assay, colony counts are shown for A549 cells treated with 0, 2, 4, and 6 Gy of radiation, with or without LB1, at 0, 25, 50, and 75 µM. (H) colony count for A549 and Lewis lung carcinoma (LLC1) cell line , respectively. Graphs are generated from the average of at least 3 study repeats. The SD of the multiple independent repeats is shown, while statistical significance is shown by *** P < .001. Abbreviation : OD = optical density; OH = hydroxide ion; MTT = methyl dimethylthiazol diphenyltetrazolium bromide; RT = radiation therapy.

    Article Snippet: A549 cells were cultured in RPMI cell culture media (Gibco), the HT29 cell line was cultured in McCoy’s 5A medium (ATCC), and the LLC1 cell was cultured in Dulbecco’s Modified Eagle Media (Gibco).

    Techniques: In Vitro, Growth Assay, Clonogenic Cell Survival Assay, Generated

    oHSV-1 exhibits the characteristics of a standard one-step growth curve and can efficiently replicate and express exogenous genes (A) The results of virus titer determination of HSV-1/WT, RG2001m, RG2002m, RG2005m, RG2011m, and RG2012m in Vero cells 48 h after infection. (B) Vero cells were infected with oHSV-1 at a multiplicity of infection (MOI) = 1, and the virus titers were determined at 0, 8, 16, 24, 32, 40, and 48 h, respectively, and a growth curve was drawn. (C) The results of virus titer determination of oHSV-1 in colon cancer cells (CT26, HCT116, and HT29) 48 h after infection. (D–F) The growth curves of oHSV-1 in colon cancer cells (CT26, HCT116, and HT29). (G) At MOI = 10, IL-15 expression levels in cell supernatants of HSV-1/WT, RG2001m, RG2005m, and RG2012m at 24, 48, and 72 h post-infection (mouse IL-15 ELISA Kit, elabscience). (H) At MOI = 10, IL-12 expression levels in cell supernatants of HSV-1/WT, RG2002m, RG2005m, and RG2012m at 24, 48, and 72 h post-infection (mouse IL-12 ELISA Kit, elabscience). (I) At MOI = 10, chemokine CCL5 expression levels in cell supernatants of HSV-1/WT, RG2011m, and RG2012m at 24, 48, and 72 h post-infection (mouse CCL5 ELISA Kit, elabscience). (Data are presented as mean ± SD. Comparisons between datasets were performed using one-way ANOVA and Student’s t test, with p values calculated [ n = 3]) ( p > 0.05, ns; p < 0.05, ∗; p < 0.01, ∗ ∗; p < 0.001, ∗ ∗ ∗).

    Journal: iScience

    Article Title: Triple-armed oncolytic HSV enhances antitumor immunity by remodeling the tumor microenvironment in subcutaneous murine CRC

    doi: 10.1016/j.isci.2026.115883

    Figure Lengend Snippet: oHSV-1 exhibits the characteristics of a standard one-step growth curve and can efficiently replicate and express exogenous genes (A) The results of virus titer determination of HSV-1/WT, RG2001m, RG2002m, RG2005m, RG2011m, and RG2012m in Vero cells 48 h after infection. (B) Vero cells were infected with oHSV-1 at a multiplicity of infection (MOI) = 1, and the virus titers were determined at 0, 8, 16, 24, 32, 40, and 48 h, respectively, and a growth curve was drawn. (C) The results of virus titer determination of oHSV-1 in colon cancer cells (CT26, HCT116, and HT29) 48 h after infection. (D–F) The growth curves of oHSV-1 in colon cancer cells (CT26, HCT116, and HT29). (G) At MOI = 10, IL-15 expression levels in cell supernatants of HSV-1/WT, RG2001m, RG2005m, and RG2012m at 24, 48, and 72 h post-infection (mouse IL-15 ELISA Kit, elabscience). (H) At MOI = 10, IL-12 expression levels in cell supernatants of HSV-1/WT, RG2002m, RG2005m, and RG2012m at 24, 48, and 72 h post-infection (mouse IL-12 ELISA Kit, elabscience). (I) At MOI = 10, chemokine CCL5 expression levels in cell supernatants of HSV-1/WT, RG2011m, and RG2012m at 24, 48, and 72 h post-infection (mouse CCL5 ELISA Kit, elabscience). (Data are presented as mean ± SD. Comparisons between datasets were performed using one-way ANOVA and Student’s t test, with p values calculated [ n = 3]) ( p > 0.05, ns; p < 0.05, ∗; p < 0.01, ∗ ∗; p < 0.001, ∗ ∗ ∗).

    Article Snippet: CT26, HTC116, and HT29 cells were purchased from Wuhan Procell Life Science & Technology Co., Ltd., China (Cat. No. CL-0071; Cat. No. CL-0096; Cat. No. CL-0118).

    Techniques: Virus, Infection, Expressing, Enzyme-linked Immunosorbent Assay

    Killing effect of recombinant oHSV-1 on colon cancer cells (A–C) CT26 (A), HCT116 (B), and HT29 (C) colon cancer cells were infected with recombinant oncolytic virus at multiplicity of infection (MOI) of 0.5, 1, and 5, respectively. The cell killing rate was detected by cell counting kit-8 (CCK8) assay 48 h after infection. The results showed that the killing effect of the virus on the three colon cancer cells was enhanced in an MOI - dependent manner. (D–F) CT26 (D), HCT116 (E), and HT29 (F) cells were infected with recombinant oncolytic virus at MOI = 1. The killing rate was measured by CCK8 assay at 24, 48, and 72 h after infection, suggesting that the killing effect of the virus on colon cancer cells was significantly enhanced with the prolongation of action time. (The values are presented as mean ± SD. The datasets were compared via one-way ANOVA. n = 3) ( p > 0.05, ns; p < 0.05, ∗; p < 0.01, ∗ ∗; p < 0.001, ∗ ∗ ∗.).

    Journal: iScience

    Article Title: Triple-armed oncolytic HSV enhances antitumor immunity by remodeling the tumor microenvironment in subcutaneous murine CRC

    doi: 10.1016/j.isci.2026.115883

    Figure Lengend Snippet: Killing effect of recombinant oHSV-1 on colon cancer cells (A–C) CT26 (A), HCT116 (B), and HT29 (C) colon cancer cells were infected with recombinant oncolytic virus at multiplicity of infection (MOI) of 0.5, 1, and 5, respectively. The cell killing rate was detected by cell counting kit-8 (CCK8) assay 48 h after infection. The results showed that the killing effect of the virus on the three colon cancer cells was enhanced in an MOI - dependent manner. (D–F) CT26 (D), HCT116 (E), and HT29 (F) cells were infected with recombinant oncolytic virus at MOI = 1. The killing rate was measured by CCK8 assay at 24, 48, and 72 h after infection, suggesting that the killing effect of the virus on colon cancer cells was significantly enhanced with the prolongation of action time. (The values are presented as mean ± SD. The datasets were compared via one-way ANOVA. n = 3) ( p > 0.05, ns; p < 0.05, ∗; p < 0.01, ∗ ∗; p < 0.001, ∗ ∗ ∗.).

    Article Snippet: CT26, HTC116, and HT29 cells were purchased from Wuhan Procell Life Science & Technology Co., Ltd., China (Cat. No. CL-0071; Cat. No. CL-0096; Cat. No. CL-0118).

    Techniques: Recombinant, Infection, Virus, Cell Counting, CCK-8 Assay

    oHSV-1 exhibits the characteristics of a standard one-step growth curve and can efficiently replicate and express exogenous genes (A) The results of virus titer determination of HSV-1/WT, RG2001m, RG2002m, RG2005m, RG2011m, and RG2012m in Vero cells 48 h after infection. (B) Vero cells were infected with oHSV-1 at a multiplicity of infection (MOI) = 1, and the virus titers were determined at 0, 8, 16, 24, 32, 40, and 48 h, respectively, and a growth curve was drawn. (C) The results of virus titer determination of oHSV-1 in colon cancer cells (CT26, HCT116, and HT29) 48 h after infection. (D–F) The growth curves of oHSV-1 in colon cancer cells (CT26, HCT116, and HT29). (G) At MOI = 10, IL-15 expression levels in cell supernatants of HSV-1/WT, RG2001m, RG2005m, and RG2012m at 24, 48, and 72 h post-infection (mouse IL-15 ELISA Kit, elabscience). (H) At MOI = 10, IL-12 expression levels in cell supernatants of HSV-1/WT, RG2002m, RG2005m, and RG2012m at 24, 48, and 72 h post-infection (mouse IL-12 ELISA Kit, elabscience). (I) At MOI = 10, chemokine CCL5 expression levels in cell supernatants of HSV-1/WT, RG2011m, and RG2012m at 24, 48, and 72 h post-infection (mouse CCL5 ELISA Kit, elabscience). (Data are presented as mean ± SD. Comparisons between datasets were performed using one-way ANOVA and Student’s t test, with p values calculated [ n = 3]) ( p > 0.05, ns; p < 0.05, ∗; p < 0.01, ∗ ∗; p < 0.001, ∗ ∗ ∗).

    Journal: iScience

    Article Title: Triple-armed oncolytic HSV enhances antitumor immunity by remodeling the tumor microenvironment in subcutaneous murine CRC

    doi: 10.1016/j.isci.2026.115883

    Figure Lengend Snippet: oHSV-1 exhibits the characteristics of a standard one-step growth curve and can efficiently replicate and express exogenous genes (A) The results of virus titer determination of HSV-1/WT, RG2001m, RG2002m, RG2005m, RG2011m, and RG2012m in Vero cells 48 h after infection. (B) Vero cells were infected with oHSV-1 at a multiplicity of infection (MOI) = 1, and the virus titers were determined at 0, 8, 16, 24, 32, 40, and 48 h, respectively, and a growth curve was drawn. (C) The results of virus titer determination of oHSV-1 in colon cancer cells (CT26, HCT116, and HT29) 48 h after infection. (D–F) The growth curves of oHSV-1 in colon cancer cells (CT26, HCT116, and HT29). (G) At MOI = 10, IL-15 expression levels in cell supernatants of HSV-1/WT, RG2001m, RG2005m, and RG2012m at 24, 48, and 72 h post-infection (mouse IL-15 ELISA Kit, elabscience). (H) At MOI = 10, IL-12 expression levels in cell supernatants of HSV-1/WT, RG2002m, RG2005m, and RG2012m at 24, 48, and 72 h post-infection (mouse IL-12 ELISA Kit, elabscience). (I) At MOI = 10, chemokine CCL5 expression levels in cell supernatants of HSV-1/WT, RG2011m, and RG2012m at 24, 48, and 72 h post-infection (mouse CCL5 ELISA Kit, elabscience). (Data are presented as mean ± SD. Comparisons between datasets were performed using one-way ANOVA and Student’s t test, with p values calculated [ n = 3]) ( p > 0.05, ns; p < 0.05, ∗; p < 0.01, ∗ ∗; p < 0.001, ∗ ∗ ∗).

    Article Snippet: HT29 , Wuhan Procell Life Science & Technology Co., Ltd., China , Cat. No. CL-0118; RRID: CVCL_0320.

    Techniques: Virus, Infection, Expressing, Enzyme-linked Immunosorbent Assay

    Killing effect of recombinant oHSV-1 on colon cancer cells (A–C) CT26 (A), HCT116 (B), and HT29 (C) colon cancer cells were infected with recombinant oncolytic virus at multiplicity of infection (MOI) of 0.5, 1, and 5, respectively. The cell killing rate was detected by cell counting kit-8 (CCK8) assay 48 h after infection. The results showed that the killing effect of the virus on the three colon cancer cells was enhanced in an MOI - dependent manner. (D–F) CT26 (D), HCT116 (E), and HT29 (F) cells were infected with recombinant oncolytic virus at MOI = 1. The killing rate was measured by CCK8 assay at 24, 48, and 72 h after infection, suggesting that the killing effect of the virus on colon cancer cells was significantly enhanced with the prolongation of action time. (The values are presented as mean ± SD. The datasets were compared via one-way ANOVA. n = 3) ( p > 0.05, ns; p < 0.05, ∗; p < 0.01, ∗ ∗; p < 0.001, ∗ ∗ ∗.).

    Journal: iScience

    Article Title: Triple-armed oncolytic HSV enhances antitumor immunity by remodeling the tumor microenvironment in subcutaneous murine CRC

    doi: 10.1016/j.isci.2026.115883

    Figure Lengend Snippet: Killing effect of recombinant oHSV-1 on colon cancer cells (A–C) CT26 (A), HCT116 (B), and HT29 (C) colon cancer cells were infected with recombinant oncolytic virus at multiplicity of infection (MOI) of 0.5, 1, and 5, respectively. The cell killing rate was detected by cell counting kit-8 (CCK8) assay 48 h after infection. The results showed that the killing effect of the virus on the three colon cancer cells was enhanced in an MOI - dependent manner. (D–F) CT26 (D), HCT116 (E), and HT29 (F) cells were infected with recombinant oncolytic virus at MOI = 1. The killing rate was measured by CCK8 assay at 24, 48, and 72 h after infection, suggesting that the killing effect of the virus on colon cancer cells was significantly enhanced with the prolongation of action time. (The values are presented as mean ± SD. The datasets were compared via one-way ANOVA. n = 3) ( p > 0.05, ns; p < 0.05, ∗; p < 0.01, ∗ ∗; p < 0.001, ∗ ∗ ∗.).

    Article Snippet: HT29 , Wuhan Procell Life Science & Technology Co., Ltd., China , Cat. No. CL-0118; RRID: CVCL_0320.

    Techniques: Recombinant, Infection, Virus, Cell Counting, CCK-8 Assay