rabbit anti-active caspase-3 (Becton Dickinson)
90
Structured Review
Becton Dickinson
rabbit anti-active caspase-3
Rabbit Anti Active Caspase 3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ht-12+expression+beadchip+v4+microarray/anti+caspase+3/pmc05932208-698-2-9
Average 90 stars, based on 1 article reviews
Rabbit Anti Active Caspase 3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ht-12+expression+beadchip+v4+microarray/anti+caspase+3/pmc05932208-698-2-9
Average 90 stars, based on 1 article reviews
rabbit anti-active caspase-3 - by Bioz Stars,
2026-11
90/100 stars
Images
1) Product Images from "Increased Tumor Glycolysis Characterizes Immune Resistance to Adoptive T cell Therapy"
Article Title: Increased Tumor Glycolysis Characterizes Immune Resistance to Adoptive T cell Therapy
Journal: Cell metabolism
doi: 10.1016/j.cmet.2018.02.024
Figure Legend Snippet: A. The linear regression of mRNA expression of ALDOA in patient-derived melanoma cell lines correlated with the percentage of T cell-mediated tumor killing is shown. Patient-derived melanoma cell lines (n=12) overexpressing Gp100 and H-2Db, which can be recognized by Pmel T cells derived from the TCR transgenic Pmel-1 mouse, were generated by lentivirus-based genetic manipulations. Transduced melanoma cells were co-cultured with Pmel T cells at an effector to target ratio of 10:1 for 3 hours. The percentage of T cell-induced tumor killing was then calculated by quantifying the number of cleaved caspase-3+ cells with respect to the total number of tumor cells by flow cytometry. The mRNA expression of glycolysis-related genes in patient-derived melanoma cells was determined by microarray analysis. The R and P-value of the correlation between the ALDOA expression and the sensitivity to T cell killing in this set of patient-derived melanoma cell lines are listed. B. Overexpression of ALDOA in melanoma cells promotes resistance to T cell-mediated tumor cell apoptosis. Patient-derived melanoma cells (Mel2400) were transduced with lentiviral vectors encoding either ALDOA-RFP or GFP-RFP. The percentage of cleaved caspase-3+ transduced melanoma cells (based on the expression of the reporter gene RFP) was determined by flow cytometry. C. The impaired effector function of TILs by lactic acid is shown. TILs (TIL2559) derived from a melanoma patient were treated overnight with different concentrations of lactic acid. *P<0.05 (with versus without lactic acid treatment) D. LDHAi increases T cell-induced killing of melanoma cells. Patient-derived melanoma cells (Mel2792) were pre-treated with 1 uM of GSK2837808A, and followed by co-culture with autologous TILs (TIL2792) for 4 hours. E. Targeting glycolysis pathway enhances the antitumor activity of T cells. B16 melanoma cells were implanted subcutaneously in C57BL/6 mice. Tumor-bearing mice were treated with either GSK2837808A (6 mg/kg) orally and/or adoptively transferred Pmel T cells. Mice treated with the relevant solvent were used as control. Tumor sizes were monitored by measuring the perpendicular diameters of the tumors and are shown as mean tumor area ± SEM. All experiments were carried out in a blinded, randomized fashion (n=4–5 mice per group). F. Kaplan-Maier plots show survivals of tumor-bearing mice treated with vehicle, GSK2837808A, T cell therapy and combination of GSK2837808A and T cell therapy (n=4–5 mice per group). *P<0.05, **P<0.01, and ****P<0.0001.
Techniques Used: Expressing, Derivative Assay, Transgenic Assay, Generated, Cell Culture, Flow Cytometry, Microarray, Over Expression, Transduction, Co-Culture Assay, Activity Assay
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