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22ag d[a(gggtta) 3 ggg] (purification: hplc; quality control: maldi‐tof; synthesis scale: 1.0 μmol)  (biomers.net gmbh)

 
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    Structured Review

    biomers.net gmbh 22ag d[a(gggtta) 3 ggg] (purification: hplc; quality control: maldi‐tof; synthesis scale: 1.0 μmol)
    Spectrophotometric titration of 7 b (1) and 8 b (2) with ct DNA [A, c L =10 μ m , c DNA =2.17 m m (A1), c DNA =1.49 m m (A2); c DNA in base pairs] in BPE buffer ( c Na+ =16 m m , pH 7.0; with 5 % v/v DMSO), <t>22AG</t> (B, c L =5 μ m , c 22AG =285 μ m ; c 22AG in oligonucleotide) in K‐phosphate buffer pH 7.0 ( c K+ =73 m m ; with 5 % v/v DMSO) and PSS [C, c L =10 μ m , c PSS =190 μ m (C1), c PSS =195 μ m (C2)] in BPE buffer ( c Na+ =16 m m , pH 7.0; with 2.5–5 % v/v DMSO). Red: Spectra of the pure ligand solutions; blue: spectra at the end of the titrations. The arrows indicate the changes of absorption upon addition of the host molecule. Insets: Plot of Abs./ Abs. 0 versus c DNA / c L or c PSS / c L .
    22ag D[A(gggtta) 3 Ggg] (Purification: Hplc; Quality Control: Maldi‐Tof; Synthesis Scale: 1.0 μmol), supplied by biomers.net gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hplc+system+system+controller+data+analysis+software/22ag+d+a+gggtta++3+ggg+++purification++hplc++quality+control++maldi+tof++synthesis+scale++1+0+%CE%BCmol+/pmc06973281-185-1-18
    Average 90 stars, based on 1 article reviews
    22ag d[a(gggtta) 3 ggg] (purification: hplc; quality control: maldi‐tof; synthesis scale: 1.0 μmol) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Synthesis of 5‐Alkyl‐ and 5‐Phenylamino‐Substituted Azothiazole Dyes with Solvatochromic and DNA‐Binding Properties"

    Article Title: Synthesis of 5‐Alkyl‐ and 5‐Phenylamino‐Substituted Azothiazole Dyes with Solvatochromic and DNA‐Binding Properties

    Journal: Chemistry (Weinheim an Der Bergstrasse, Germany)

    doi: 10.1002/chem.201903657

    Spectrophotometric titration of 7 b (1) and 8 b (2) with ct DNA [A, c L =10 μ m , c DNA =2.17 m m (A1), c DNA =1.49 m m (A2); c DNA in base pairs] in BPE buffer ( c Na+ =16 m m , pH 7.0; with 5 % v/v DMSO), 22AG (B, c L =5 μ m , c 22AG =285 μ m ; c 22AG in oligonucleotide) in K‐phosphate buffer pH 7.0 ( c K+ =73 m m ; with 5 % v/v DMSO) and PSS [C, c L =10 μ m , c PSS =190 μ m (C1), c PSS =195 μ m (C2)] in BPE buffer ( c Na+ =16 m m , pH 7.0; with 2.5–5 % v/v DMSO). Red: Spectra of the pure ligand solutions; blue: spectra at the end of the titrations. The arrows indicate the changes of absorption upon addition of the host molecule. Insets: Plot of Abs./ Abs. 0 versus c DNA / c L or c PSS / c L .
    Figure Legend Snippet: Spectrophotometric titration of 7 b (1) and 8 b (2) with ct DNA [A, c L =10 μ m , c DNA =2.17 m m (A1), c DNA =1.49 m m (A2); c DNA in base pairs] in BPE buffer ( c Na+ =16 m m , pH 7.0; with 5 % v/v DMSO), 22AG (B, c L =5 μ m , c 22AG =285 μ m ; c 22AG in oligonucleotide) in K‐phosphate buffer pH 7.0 ( c K+ =73 m m ; with 5 % v/v DMSO) and PSS [C, c L =10 μ m , c PSS =190 μ m (C1), c PSS =195 μ m (C2)] in BPE buffer ( c Na+ =16 m m , pH 7.0; with 2.5–5 % v/v DMSO). Red: Spectra of the pure ligand solutions; blue: spectra at the end of the titrations. The arrows indicate the changes of absorption upon addition of the host molecule. Insets: Plot of Abs./ Abs. 0 versus c DNA / c L or c PSS / c L .

    Techniques Used: Titration

    Absorption and Emission Properties of 7 b and 8 b in the Presence of ct DNA,  22AG  and PSS.
    Figure Legend Snippet: Absorption and Emission Properties of 7 b and 8 b in the Presence of ct DNA, 22AG and PSS.

    Techniques Used:

    Spectrofluorimetric titration of 8 b with ct DNA (A, c L =10 μ m , c DNA =1.49 m m ; c DNA in base pairs) in BPE buffer ( c Na+ =16 m m , pH 7.0; with 5 % v/v DMSO), 22AG (B, c L =5 μ m , c 22AG =285 μ m ; c 22AG in oligonucleotide) in K‐phosphate buffer ( c K+ =73 m m , pH 7.0; with 5 % v/v DMSO) and PSS (C, c L =10 μ m , c PSS =195 μ m ) in BPE buffer ( c Na+ =16 m m , pH 7.0; with 2.5 % v/v DMSO); λ ex =515 nm. Red: Spectra of the pure ligand solutions; blue: spectra at the end of the titrations. The arrows indicate the changes in emission intensity upon addition of the host molecule. Insets: Plot of the relative fluorescence intensity I / I 0 (corrected with regard to the change of the absorption at the excitation wavelength) versus c DNA / c L or c PSS / c L . Inset pictures in A: Fluorescence colors of 8 b in the absence and in the presence of ct DNA; λ ex =366 nm. The contrast and brightness were enhanced by 30 % without changing the true colors (cf. Figure S5).
    Figure Legend Snippet: Spectrofluorimetric titration of 8 b with ct DNA (A, c L =10 μ m , c DNA =1.49 m m ; c DNA in base pairs) in BPE buffer ( c Na+ =16 m m , pH 7.0; with 5 % v/v DMSO), 22AG (B, c L =5 μ m , c 22AG =285 μ m ; c 22AG in oligonucleotide) in K‐phosphate buffer ( c K+ =73 m m , pH 7.0; with 5 % v/v DMSO) and PSS (C, c L =10 μ m , c PSS =195 μ m ) in BPE buffer ( c Na+ =16 m m , pH 7.0; with 2.5 % v/v DMSO); λ ex =515 nm. Red: Spectra of the pure ligand solutions; blue: spectra at the end of the titrations. The arrows indicate the changes in emission intensity upon addition of the host molecule. Insets: Plot of the relative fluorescence intensity I / I 0 (corrected with regard to the change of the absorption at the excitation wavelength) versus c DNA / c L or c PSS / c L . Inset pictures in A: Fluorescence colors of 8 b in the absence and in the presence of ct DNA; λ ex =366 nm. The contrast and brightness were enhanced by 30 % without changing the true colors (cf. Figure S5).

    Techniques Used: Titration, Fluorescence

    Related Articles

    other:

    Article Title:
    Article Snippet: The oligodeoxyribonucleotide 22AG d[A(GGGTTA) 3 GGG] (purification: HPLC; quality control: MALDI‐TOF; synthesis scale: 1.0 μmol) was purchased from biomers.net GmbH (Ulm, Germany).



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