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hpld  (ATCC)


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  • 99

    Structured Review

    ATCC hpld
    Proliferation was measured by the MTT assay. (A) VEGFq, but not MutVEGFq inhibited the growth of A459 cells in a concentration and time dependent fashion. (B) VEGFq also inhibited the growth of the H1299 and H1944 NSCLC cell lines, H3255 squamous cell lung cancer cells, and the Calu-1 <t>epidermoid</t> <t>carcinoma</t> cell line, but had very little effect on the non-transformed foreskin fibroblast Hs27 cells and the lung epithelial cells, <t>HPLD-1</t> measured at 144 h. Bars represent mean±SEM absorbance of three separate determinations. * indicates (p<0.05) compared to untreated cells.
    Hpld, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4446 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hplc+system+system+controller+data+analysis+software/L+Cells/pmc06347295-35-30-36
    Average 99 stars, based on 4446 article reviews
    hpld - by Bioz Stars, 2026-10
    99/100 stars

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    1) Product Images from "Quadruplex-forming oligonucleotide targeted to the VEGF promoter inhibits growth of non-small cell lung cancer cells"

    Article Title: Quadruplex-forming oligonucleotide targeted to the VEGF promoter inhibits growth of non-small cell lung cancer cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0211046

    Proliferation was measured by the MTT assay. (A) VEGFq, but not MutVEGFq inhibited the growth of A459 cells in a concentration and time dependent fashion. (B) VEGFq also inhibited the growth of the H1299 and H1944 NSCLC cell lines, H3255 squamous cell lung cancer cells, and the Calu-1 epidermoid carcinoma cell line, but had very little effect on the non-transformed foreskin fibroblast Hs27 cells and the lung epithelial cells, HPLD-1 measured at 144 h. Bars represent mean±SEM absorbance of three separate determinations. * indicates (p<0.05) compared to untreated cells.
    Figure Legend Snippet: Proliferation was measured by the MTT assay. (A) VEGFq, but not MutVEGFq inhibited the growth of A459 cells in a concentration and time dependent fashion. (B) VEGFq also inhibited the growth of the H1299 and H1944 NSCLC cell lines, H3255 squamous cell lung cancer cells, and the Calu-1 epidermoid carcinoma cell line, but had very little effect on the non-transformed foreskin fibroblast Hs27 cells and the lung epithelial cells, HPLD-1 measured at 144 h. Bars represent mean±SEM absorbance of three separate determinations. * indicates (p<0.05) compared to untreated cells.

    Techniques Used: MTT Assay, Concentration Assay, Transformation Assay

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    other:

    Article Title: Engineered antibodies that bind LAG3
    Article Snippet: Examples of host cells include the COS-7 line of monkey kidney cells (ATCC CRL 1651) (see Gluzman et al., 1981, Cell 23: 175), L cells, C127 cells, 3T3 cells (ATCC CCL 163), Chinese hamster ovary (CHO) cells or their derivatives such as Veggie CHO and related cell lines which grow in serum-free media (see Rasmussen et al., 1998, Cytotechnology 28:31) or CHO strain DX-B 11, which is deficient in DHFR (see Urlaub et al., 1980, Proc.

    Article Title: Variant antibody that binds CD38
    Article Snippet: Examples of host cells include the COS-7 line of monkey kidney cells (ATCC CRL 1651) (see Gluzman et al., 1981, Cell 23: 175), L cells, C127 cells, 3T3 cells (ATCC CCL 163), Chinese hamster ovary (CHO) cells or their derivatives such as Veggie CHO and related cell lines which grow in serum-free media (see Rasmussen et al., 1998, Cytotechnology 28:31) or CHO strain DX-B 11, which is deficient in DHFR (see Urlaub et al., 1980, Proc.

    Article Title: Cells, islets, and organoids that evade immune detection and autoimmunity, methods of production and use thereof
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    Article Title: Antigen binding proteins that bind BCMA
    Article Snippet: Examples of host cells include the COS-7 line of monkey kidney cells (ATCC CRL 1651) (see Gluzman et al., 1981, Cell 23: 175), L cells, C127 cells, 3T3 cells (ATCC CCL 163), Chinese hamster ovary (CHO) cells or their derivatives such as Veggie CHO and related cell lines which grow in serum-free media (see Rasmussen et al., 1998, Cytotechnology 28:31) or CHO strain DX-B 11, which is deficient in DHFR (see Urlaub et al., 1980, Proc.

    Isolation:

    Article Title: Anti-CCR8 antibodies
    Article Snippet: Many mammalian cell lines suitable for expression of heterologous polypeptides are known in the art and can be obtained from a variety of vendors including, e.g., American Type Culture Collection (ATCC). .. Suitable mammalian host cell lines include, for example, the COS-7 line (ATCC CRL 1651) (Gluzman et al., (1981), SV40-transformed simian cells support the replication of early SV40 mutants, Cell 23 (1): 175-182), L cells, C127 cells, 3T3 cells (ATCC CCL 163), Chinese hamster ovary (CHO) cells, or their derivatives such as Veggie CHO and related cell lines, which grow in serum-free media (Rasmussen et al. (1998), Isolation, characterization and recombinant protein expression in Veggie-CHO: a serum-free CHO host cell line, Cytotechnology 28:31), CHO-K1 and CHO pro-3 cell lines and their derivatives such as the DUKX-X11 and DG44 cell lines, which are deficient in dihydrofolate reductase (DHFR) activity, Hela cells, baby hamster kidney (BHK) cells (e.g., ATCC CRL 10), the CVI/EBNA cell line derived from the African green monkey kidney cell line CVI (ATCC CCL 70) as described by McMahan et al. (1991), A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types, EMBO J. .. 10 (10): 2821-2832, human embryonic kidney (HEK) cells such as HEK 293, 293 EBNA or MSR 293, human epidermal A431 cells, human Colo205 cells, HL-60 cells, U937 cells, Hak cells, Jurkat cells, HepG2/3B cells, KB cells, NIH 3T3 cells, S49 cells, PER.C6 (Crucell), CAP and CAP-T cells (CEVEC), and mouse myeloma cells, including NSO and Sp2/0 cells.

    Recombinant:

    Article Title: Anti-CCR8 antibodies
    Article Snippet: Many mammalian cell lines suitable for expression of heterologous polypeptides are known in the art and can be obtained from a variety of vendors including, e.g., American Type Culture Collection (ATCC). .. Suitable mammalian host cell lines include, for example, the COS-7 line (ATCC CRL 1651) (Gluzman et al., (1981), SV40-transformed simian cells support the replication of early SV40 mutants, Cell 23 (1): 175-182), L cells, C127 cells, 3T3 cells (ATCC CCL 163), Chinese hamster ovary (CHO) cells, or their derivatives such as Veggie CHO and related cell lines, which grow in serum-free media (Rasmussen et al. (1998), Isolation, characterization and recombinant protein expression in Veggie-CHO: a serum-free CHO host cell line, Cytotechnology 28:31), CHO-K1 and CHO pro-3 cell lines and their derivatives such as the DUKX-X11 and DG44 cell lines, which are deficient in dihydrofolate reductase (DHFR) activity, Hela cells, baby hamster kidney (BHK) cells (e.g., ATCC CRL 10), the CVI/EBNA cell line derived from the African green monkey kidney cell line CVI (ATCC CCL 70) as described by McMahan et al. (1991), A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types, EMBO J. .. 10 (10): 2821-2832, human embryonic kidney (HEK) cells such as HEK 293, 293 EBNA or MSR 293, human epidermal A431 cells, human Colo205 cells, HL-60 cells, U937 cells, Hak cells, Jurkat cells, HepG2/3B cells, KB cells, NIH 3T3 cells, S49 cells, PER.C6 (Crucell), CAP and CAP-T cells (CEVEC), and mouse myeloma cells, including NSO and Sp2/0 cells.

    Expressing:

    Article Title: Anti-CCR8 antibodies
    Article Snippet: Many mammalian cell lines suitable for expression of heterologous polypeptides are known in the art and can be obtained from a variety of vendors including, e.g., American Type Culture Collection (ATCC). .. Suitable mammalian host cell lines include, for example, the COS-7 line (ATCC CRL 1651) (Gluzman et al., (1981), SV40-transformed simian cells support the replication of early SV40 mutants, Cell 23 (1): 175-182), L cells, C127 cells, 3T3 cells (ATCC CCL 163), Chinese hamster ovary (CHO) cells, or their derivatives such as Veggie CHO and related cell lines, which grow in serum-free media (Rasmussen et al. (1998), Isolation, characterization and recombinant protein expression in Veggie-CHO: a serum-free CHO host cell line, Cytotechnology 28:31), CHO-K1 and CHO pro-3 cell lines and their derivatives such as the DUKX-X11 and DG44 cell lines, which are deficient in dihydrofolate reductase (DHFR) activity, Hela cells, baby hamster kidney (BHK) cells (e.g., ATCC CRL 10), the CVI/EBNA cell line derived from the African green monkey kidney cell line CVI (ATCC CCL 70) as described by McMahan et al. (1991), A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types, EMBO J. .. 10 (10): 2821-2832, human embryonic kidney (HEK) cells such as HEK 293, 293 EBNA or MSR 293, human epidermal A431 cells, human Colo205 cells, HL-60 cells, U937 cells, Hak cells, Jurkat cells, HepG2/3B cells, KB cells, NIH 3T3 cells, S49 cells, PER.C6 (Crucell), CAP and CAP-T cells (CEVEC), and mouse myeloma cells, including NSO and Sp2/0 cells.

    Activity Assay:

    Article Title: Anti-CCR8 antibodies
    Article Snippet: Many mammalian cell lines suitable for expression of heterologous polypeptides are known in the art and can be obtained from a variety of vendors including, e.g., American Type Culture Collection (ATCC). .. Suitable mammalian host cell lines include, for example, the COS-7 line (ATCC CRL 1651) (Gluzman et al., (1981), SV40-transformed simian cells support the replication of early SV40 mutants, Cell 23 (1): 175-182), L cells, C127 cells, 3T3 cells (ATCC CCL 163), Chinese hamster ovary (CHO) cells, or their derivatives such as Veggie CHO and related cell lines, which grow in serum-free media (Rasmussen et al. (1998), Isolation, characterization and recombinant protein expression in Veggie-CHO: a serum-free CHO host cell line, Cytotechnology 28:31), CHO-K1 and CHO pro-3 cell lines and their derivatives such as the DUKX-X11 and DG44 cell lines, which are deficient in dihydrofolate reductase (DHFR) activity, Hela cells, baby hamster kidney (BHK) cells (e.g., ATCC CRL 10), the CVI/EBNA cell line derived from the African green monkey kidney cell line CVI (ATCC CCL 70) as described by McMahan et al. (1991), A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types, EMBO J. .. 10 (10): 2821-2832, human embryonic kidney (HEK) cells such as HEK 293, 293 EBNA or MSR 293, human epidermal A431 cells, human Colo205 cells, HL-60 cells, U937 cells, Hak cells, Jurkat cells, HepG2/3B cells, KB cells, NIH 3T3 cells, S49 cells, PER.C6 (Crucell), CAP and CAP-T cells (CEVEC), and mouse myeloma cells, including NSO and Sp2/0 cells.

    Derivative Assay:

    Article Title: Anti-CCR8 antibodies
    Article Snippet: Many mammalian cell lines suitable for expression of heterologous polypeptides are known in the art and can be obtained from a variety of vendors including, e.g., American Type Culture Collection (ATCC). .. Suitable mammalian host cell lines include, for example, the COS-7 line (ATCC CRL 1651) (Gluzman et al., (1981), SV40-transformed simian cells support the replication of early SV40 mutants, Cell 23 (1): 175-182), L cells, C127 cells, 3T3 cells (ATCC CCL 163), Chinese hamster ovary (CHO) cells, or their derivatives such as Veggie CHO and related cell lines, which grow in serum-free media (Rasmussen et al. (1998), Isolation, characterization and recombinant protein expression in Veggie-CHO: a serum-free CHO host cell line, Cytotechnology 28:31), CHO-K1 and CHO pro-3 cell lines and their derivatives such as the DUKX-X11 and DG44 cell lines, which are deficient in dihydrofolate reductase (DHFR) activity, Hela cells, baby hamster kidney (BHK) cells (e.g., ATCC CRL 10), the CVI/EBNA cell line derived from the African green monkey kidney cell line CVI (ATCC CCL 70) as described by McMahan et al. (1991), A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types, EMBO J. .. 10 (10): 2821-2832, human embryonic kidney (HEK) cells such as HEK 293, 293 EBNA or MSR 293, human epidermal A431 cells, human Colo205 cells, HL-60 cells, U937 cells, Hak cells, Jurkat cells, HepG2/3B cells, KB cells, NIH 3T3 cells, S49 cells, PER.C6 (Crucell), CAP and CAP-T cells (CEVEC), and mouse myeloma cells, including NSO and Sp2/0 cells.

    Clone Assay:

    Article Title: Anti-CCR8 antibodies
    Article Snippet: Many mammalian cell lines suitable for expression of heterologous polypeptides are known in the art and can be obtained from a variety of vendors including, e.g., American Type Culture Collection (ATCC). .. Suitable mammalian host cell lines include, for example, the COS-7 line (ATCC CRL 1651) (Gluzman et al., (1981), SV40-transformed simian cells support the replication of early SV40 mutants, Cell 23 (1): 175-182), L cells, C127 cells, 3T3 cells (ATCC CCL 163), Chinese hamster ovary (CHO) cells, or their derivatives such as Veggie CHO and related cell lines, which grow in serum-free media (Rasmussen et al. (1998), Isolation, characterization and recombinant protein expression in Veggie-CHO: a serum-free CHO host cell line, Cytotechnology 28:31), CHO-K1 and CHO pro-3 cell lines and their derivatives such as the DUKX-X11 and DG44 cell lines, which are deficient in dihydrofolate reductase (DHFR) activity, Hela cells, baby hamster kidney (BHK) cells (e.g., ATCC CRL 10), the CVI/EBNA cell line derived from the African green monkey kidney cell line CVI (ATCC CCL 70) as described by McMahan et al. (1991), A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types, EMBO J. .. 10 (10): 2821-2832, human embryonic kidney (HEK) cells such as HEK 293, 293 EBNA or MSR 293, human epidermal A431 cells, human Colo205 cells, HL-60 cells, U937 cells, Hak cells, Jurkat cells, HepG2/3B cells, KB cells, NIH 3T3 cells, S49 cells, PER.C6 (Crucell), CAP and CAP-T cells (CEVEC), and mouse myeloma cells, including NSO and Sp2/0 cells.

    Control:

    Article Title: DP103 as a critical modulator of Wnt signaling and cancer stemness: implications for precision treatment in triple negative breast cancer.
    Article Snippet: Epidermal growth factor (EGF), basic FGF (bFGF), bovine insulin and B27 were purchased from Life Technology, USA. .. Wnt-3a conditioned media preparation Control and Wnt-3a conditioned media were prepared from L cells (ATCC CRL-2648) and L Wnt3A cells (ATCC CRL-2647) respectively, through harvesting spent media from 80% confluent cells, after 72 hours. ..



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