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simplyrna tissue kit homogenization buffer with 1-thioglycerol  (Promega)

 
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    Structured Review

    Promega simplyrna tissue kit homogenization buffer with 1-thioglycerol
    Simplyrna Tissue Kit Homogenization Buffer With 1 Thioglycerol, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/homogenization+buffer/simplyrna+tissue+kit+homogenization+buffer+with+1+thioglycerol/pmc10027183-58-14-22
    Average 90 stars, based on 1 article reviews
    simplyrna tissue kit homogenization buffer with 1-thioglycerol - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Effect of polymorphism in Rhinolophus affinis ACE2 on entry of SARS-CoV-2 related bat coronaviruses
    Article Snippet: .. The next day, cells were inoculated with 500 μl viral supernatant, followed by spinning-inoculation at 800×g for 30 min. After overnight incubation, cells were fed with fresh medium, and cells were lysed with 120 μl of lysis buffer with 1:1 ratio of medium and Steady-glo (Promega, Madison, USA) at 48 hrs post-inoculation. .. The luciferase activities were quantified by using a Modulus II microplate reader (Turner Biosystems, Sunnyvale, USA).

    Lysis:

    Article Title: Effect of polymorphism in Rhinolophus affinis ACE2 on entry of SARS-CoV-2 related bat coronaviruses
    Article Snippet: .. The next day, cells were inoculated with 500 μl viral supernatant, followed by spinning-inoculation at 800×g for 30 min. After overnight incubation, cells were fed with fresh medium, and cells were lysed with 120 μl of lysis buffer with 1:1 ratio of medium and Steady-glo (Promega, Madison, USA) at 48 hrs post-inoculation. .. The luciferase activities were quantified by using a Modulus II microplate reader (Turner Biosystems, Sunnyvale, USA).

    Article Title: Cnih3 Deletion Dysregulates Dorsal Hippocampal Transcription across the Estrous Cycle
    Article Snippet: .. This was mixed with 67 μl of Promega’s simplyRNA Tissue kit Homogenization buffer with 1-thioglycerol (20 μl/ml), then 200 μl of the Promega kit’s lysis buffer, and extracted using a Maxwell RSC 48 robot (Promega) following the manufacturer’s instructions. .. Total RNA integrity was determined using Agilent Bioanalyzer or 4200 Tapestation.

    Article Title: Identification of Tumor‐Specific Surface Proteins Enables Quantification of Extracellular Vesicle Subtypes for Early Detection of Pancreatic Ductal Adenocarcinoma
    Article Snippet: .. Enriched PDAC EVs captured by EV Click Bead were lysed using XpressAmp Lysis Buffer with 1% Thioglycerol (Promega, USA). .. The lysate was subjected to one‐step RT‐qPCR using PrimeDirect Probe RT‐qPCR Mix (Takara, Japan) with ACTB primers and probes, and analyzed using a CFX Duet Real‐Time PCR System (Bio‐Rad, USA).

    Article Title: Identification of Tumor-Specific Surface Proteins Enables Quantification of Extracellular Vesicle Subtypes for Early Detection of Pancreatic Ductal Adenocarcinoma.
    Article Snippet: .. Quantification of PDAC EVs by Detecting ACTB mRNA via RT-qPCR: Enriched PDAC EVs captured by EV Click Bead were lysed using XpressAmp Lysis Buffer with 1% Thioglycerol (Promega, USA). .. The lysate was subjected to one-step RT-qPCR using PrimeDirect Probe RT-qPCR Mix (Takara, Japan) with ACTB primers and probes, and analyzed using a CFX Duet Real-Time PCR System (Bio-Rad, USA).

    Article Title: Cnih3 Deletion Dysregulates Dorsal Hippocampal Transcription across the Estrous Cycle
    Article Snippet: .. This was mixed with 67 μL of Promega’s simplyRNA Tissue Kit Homogenization buffer with 1-thioglycerol (20 μL per mL), then 200 μL of Promega’s lysis, and extracted using a Maxwell RSC 48 robot (Promega) following the manufacturer’s instructions for the above Kit.” f. The use of a small amount of RNA (10ng) for library prep should be explained, since this was bulk tissue from which much more RNA can be recovered. ..

    Article Title: Apicoplast-derived isoprenoids are essential for biosynthesis of GPI protein anchors, and consequently for egress and invasion in Plasmodium falciparum.
    Article Snippet: .. 45 μL of 1x NanoGlo Lysis buffer with 1:1000 NanoGlo substrate (Promega, USA) was added. ..

    Homogenization:

    Article Title: Cnih3 Deletion Dysregulates Dorsal Hippocampal Transcription across the Estrous Cycle
    Article Snippet: .. This was mixed with 67 μl of Promega’s simplyRNA Tissue kit Homogenization buffer with 1-thioglycerol (20 μl/ml), then 200 μl of the Promega kit’s lysis buffer, and extracted using a Maxwell RSC 48 robot (Promega) following the manufacturer’s instructions. .. Total RNA integrity was determined using Agilent Bioanalyzer or 4200 Tapestation.

    Article Title: Cnih3 Deletion Dysregulates Dorsal Hippocampal Transcription across the Estrous Cycle
    Article Snippet: .. This was mixed with 67 μL of Promega’s simplyRNA Tissue Kit Homogenization buffer with 1-thioglycerol (20 μL per mL), then 200 μL of Promega’s lysis, and extracted using a Maxwell RSC 48 robot (Promega) following the manufacturer’s instructions for the above Kit.” f. The use of a small amount of RNA (10ng) for library prep should be explained, since this was bulk tissue from which much more RNA can be recovered. ..

    other:

    Article Title: Agnostic B cell selection approach identifies antibodies against K. pneumoniae that synergistically drive complement activation
    Article Snippet: Flow cytometry staining buffer consisted of DPBS (PBS-1A, Capricorn) supplemented with 3% heat-inactivated fetal calf serum (S181H, Biowest).

    HAT Assay:

    Article Title: Formation of pericentromeric heterochromatin via ZNF518 that links satellite DNA to heterochromatin
    Article Snippet: A cell extract was prepared using a protein purification buffer containing 50 mM of Tris-HCl (pH 8.0; #201-06273, Wako), 500 mM of NaCl (#191-01665, Wako), 10 mM of β-mercaptoethanol (#131-14571, Wako), and 10% glycerol (#075-00611, Wako) for the His 6 -GST-HP1 protein. .. For the HAT-MBP-ZNF518B 770-987 protein, a cell extract was prepared using the protein purification buffer with 1% Tween 20 (#H5152, Promega). .. The target proteins were then affinity-purified using Ni-NTA agarose (#143-09763, Wako).

    Protein Purification:

    Article Title: Formation of pericentromeric heterochromatin via ZNF518 that links satellite DNA to heterochromatin
    Article Snippet: A cell extract was prepared using a protein purification buffer containing 50 mM of Tris-HCl (pH 8.0; #201-06273, Wako), 500 mM of NaCl (#191-01665, Wako), 10 mM of β-mercaptoethanol (#131-14571, Wako), and 10% glycerol (#075-00611, Wako) for the His 6 -GST-HP1 protein. .. For the HAT-MBP-ZNF518B 770-987 protein, a cell extract was prepared using the protein purification buffer with 1% Tween 20 (#H5152, Promega). .. The target proteins were then affinity-purified using Ni-NTA agarose (#143-09763, Wako).



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