homogenization buffer (Thermo Fisher)

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Thermo Fisher homogenization buffer
Homogenization Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/homogenization+buffer/Sucrose/pm42021164-82-8-26
Average 99 stars, based on 1 article reviews

homogenization buffer - by Bioz Stars, 2026-10

99/100 stars

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Homogenization:

Article Title: Adaptation of lipid metabolism of the polyextremophilic cyanobacterium Cyanobacterium aponinum PCC 10605 to adverse environmental conditions.
Article Snippet: .. E. coli cells were harvested and suspended in homogenization buffer (1 mM EDTA, 200 mM sucrose, 100 mM Tris-HCl, pH 7.4) and homogenized with a sonicator (Bioblock Scientific). ..

Article Title: Adaptation of lipid metabolism of the polyextremophilic cyanobacterium Cyanobacterium aponinum PCC 10605 to adverse environmental conditions
Article Snippet: .. E. coli cells were harvested and suspended in homogenization buffer (1 mM EDTA, 200 mM sucrose, 100 mM Tris-HCl, pH 7.4) and homogenized with a sonicator (Bioblock Scientific). ..

Saline:

Article Title: The in-vitro effect of bovine milk, and plant-based alternatives on oral microorganisms and biofilms.
Article Snippet: .. Wells of two 24-well plates were coated with 100 μL of test substances or 0.9% w/v NaCl for 15 min. Then 50 μL of protein solution (1.5% bovine serum albumin/0.67% mucin in phosphate buffered saline (PBS)) was added for another 15 min, before 850 μl of bacterial suspension mixed with nutrient broth (“cariogenic”: BHI broth with 5% sucrose; periodontal: Wilkins-Chalgren broth, Oxoid, Basingstoke, GB) in a ratio (volume 1:9) was added. (Before, all precultivated bacterial strains were suspended in 0.9% w/v NaCl according to McFarland 4. .. One part of S. gordonii ATCC 10558, two parts of A. naeslundii ATCC 12104 and each four parts of the other bacteria were mixed for the bacterial suspensions.)

Suspension:

Binding Assay:

Article Title: Epistatic interaction between ribosome-associated Era GTPase and stringent response regulator RelA modulates bacterial cell growth and dormancy
Article Snippet: .. The binding reactions were incubated for 15 min at 370C in a water bath. subsequently, the reaction mixture was layered onto a 1.5 ml 15% sucrose cushion prepared in buffer RS (20 mM Tris–HCl (pH 7.5 at 25°C), 60 mM NH 4 Cl, 10 mM Mg(CH3COO)2 and 4 mM β-ME) to pellet the ribosomes by ultracentrifugation at 200000 x g at 40C for 2 hr using the F50L-24×1.5 (Thermofisher) rotor. ..

Incubation:

Article Title: Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit.
Article Snippet: .. The following day slices Invitrogen were washed with PB 4x for 30 minutes and incubated in a 1 mg ml−1 stock solution of DAPI (4',6minutes. phenylindole, dilactate) diluted 1:10,000 in PB (Invitrogen) for 10 2 diamidino % sucrose, 25% Subsequently, slices were cleared by immersing them in CUBIC solution (5 ..

other:

Article Title: Formulations comprising PCSK9 specific monoclonal antibodies
Article Snippet: Separately, 0.4 μg/mL of 11F1 was incubated for one hour at room temperature with various concentrations (ranging from 0 to 20 μg/mL) of either PCSK1, PCSK2, PCSK7, PCSK9 or furin (R&D Systems, Minneapolis, Minn.) (diluted in buffer A w/o tween).

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