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histone h2b coding sequence  (Addgene inc)


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    Structured Review

    Addgene inc histone h2b coding sequence
    Histone H2b Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/histone+h2b+coding+sequence/H2B-GFP+(Plasmid+%2311680)/pm41895260-276-1-8
    Average 95 stars, based on 199 article reviews
    histone h2b coding sequence - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    CRISPR:

    Article Title: U2 snRNP inhibition hampers pancreatic cancer cell viability by disrupting DNA repair proficiency and mitotic checkpoint integrity.
    Article Snippet: Cells were treated with Pladienolide B (Santa Cruz; sc-391691), Thailanstatin A (MedChemExpress; HY-129589), Indisulam (MedChemExpress; HY-13650), CHEK1 inhibitor (AZD7762), Alisertib (Selleckem; S1133), Barisertib (Selleckem; S1147), Talazoparib (Pfizer; CAS number 1373431-65-2) at the indicated doses, or dimethylsulphoxide (DMSO) as a control. .. CRISPR/Cas9-based engineering of PANC-1 cells For the generation of PANC-1 cells stably expressing H2B-GFP and Tubulin-RFP, we employed the following plasmids: H2BC11-GFP: pX330-PITCh-H2BC11 Cas9 plasmid (Addgene # 207755) and qTAG-C-moxGFPPuro-H2BC11-MMEJ repair plasmid (Addgene # 207760); TUBULIN-RED: pX330-PITCh-TUBA1B Cas9 plasmid (Addgene # 207763) and qTAG-N-BlastsTagRFP-TUBA1B repair plasmid (Addgene # 207768). ..

    Stable Transfection:

    Article Title: U2 snRNP inhibition hampers pancreatic cancer cell viability by disrupting DNA repair proficiency and mitotic checkpoint integrity.
    Article Snippet: Cells were treated with Pladienolide B (Santa Cruz; sc-391691), Thailanstatin A (MedChemExpress; HY-129589), Indisulam (MedChemExpress; HY-13650), CHEK1 inhibitor (AZD7762), Alisertib (Selleckem; S1133), Barisertib (Selleckem; S1147), Talazoparib (Pfizer; CAS number 1373431-65-2) at the indicated doses, or dimethylsulphoxide (DMSO) as a control. .. CRISPR/Cas9-based engineering of PANC-1 cells For the generation of PANC-1 cells stably expressing H2B-GFP and Tubulin-RFP, we employed the following plasmids: H2BC11-GFP: pX330-PITCh-H2BC11 Cas9 plasmid (Addgene # 207755) and qTAG-C-moxGFPPuro-H2BC11-MMEJ repair plasmid (Addgene # 207760); TUBULIN-RED: pX330-PITCh-TUBA1B Cas9 plasmid (Addgene # 207763) and qTAG-N-BlastsTagRFP-TUBA1B repair plasmid (Addgene # 207768). ..

    Article Title: Periodic confined cell migration drives partially reversible chromatin reorganization in cancer cell lines
    Article Snippet: .. Both cell types were transfected with a plasmid encoding the H2B-GFP fusion protein from Geoff Wahl (Addgene plasmid #11680) to generate a stable cell line constitutively expressing human histone H2B, one of the core proteins in DNA packaging, tagged with Green Fluorescent Protein (GFP). ..

    Article Title: Periodic confined cell migration drives partially reversible chromatin reorganization in cancer cell lines.
    Article Snippet: .. Both cell types were transfected with a plasmid encoding the H2B-GFP fusion protein from Geoff Wahl (Addgene plasmid #11680) to generate a stable cell line constitutively expressing human histone H2B, one of the core proteins in DNA packaging, tagged with Green Fluorescent Protein (GFP). ..

    Expressing:

    Article Title: U2 snRNP inhibition hampers pancreatic cancer cell viability by disrupting DNA repair proficiency and mitotic checkpoint integrity.
    Article Snippet: Cells were treated with Pladienolide B (Santa Cruz; sc-391691), Thailanstatin A (MedChemExpress; HY-129589), Indisulam (MedChemExpress; HY-13650), CHEK1 inhibitor (AZD7762), Alisertib (Selleckem; S1133), Barisertib (Selleckem; S1147), Talazoparib (Pfizer; CAS number 1373431-65-2) at the indicated doses, or dimethylsulphoxide (DMSO) as a control. .. CRISPR/Cas9-based engineering of PANC-1 cells For the generation of PANC-1 cells stably expressing H2B-GFP and Tubulin-RFP, we employed the following plasmids: H2BC11-GFP: pX330-PITCh-H2BC11 Cas9 plasmid (Addgene # 207755) and qTAG-C-moxGFPPuro-H2BC11-MMEJ repair plasmid (Addgene # 207760); TUBULIN-RED: pX330-PITCh-TUBA1B Cas9 plasmid (Addgene # 207763) and qTAG-N-BlastsTagRFP-TUBA1B repair plasmid (Addgene # 207768). ..

    Article Title: Periodic confined cell migration drives partially reversible chromatin reorganization in cancer cell lines
    Article Snippet: .. Both cell types were transfected with a plasmid encoding the H2B-GFP fusion protein from Geoff Wahl (Addgene plasmid #11680) to generate a stable cell line constitutively expressing human histone H2B, one of the core proteins in DNA packaging, tagged with Green Fluorescent Protein (GFP). ..

    Article Title: Periodic confined cell migration drives partially reversible chromatin reorganization in cancer cell lines.
    Article Snippet: .. Both cell types were transfected with a plasmid encoding the H2B-GFP fusion protein from Geoff Wahl (Addgene plasmid #11680) to generate a stable cell line constitutively expressing human histone H2B, one of the core proteins in DNA packaging, tagged with Green Fluorescent Protein (GFP). ..

    Plasmid Preparation:

    Article Title: U2 snRNP inhibition hampers pancreatic cancer cell viability by disrupting DNA repair proficiency and mitotic checkpoint integrity.
    Article Snippet: Cells were treated with Pladienolide B (Santa Cruz; sc-391691), Thailanstatin A (MedChemExpress; HY-129589), Indisulam (MedChemExpress; HY-13650), CHEK1 inhibitor (AZD7762), Alisertib (Selleckem; S1133), Barisertib (Selleckem; S1147), Talazoparib (Pfizer; CAS number 1373431-65-2) at the indicated doses, or dimethylsulphoxide (DMSO) as a control. .. CRISPR/Cas9-based engineering of PANC-1 cells For the generation of PANC-1 cells stably expressing H2B-GFP and Tubulin-RFP, we employed the following plasmids: H2BC11-GFP: pX330-PITCh-H2BC11 Cas9 plasmid (Addgene # 207755) and qTAG-C-moxGFPPuro-H2BC11-MMEJ repair plasmid (Addgene # 207760); TUBULIN-RED: pX330-PITCh-TUBA1B Cas9 plasmid (Addgene # 207763) and qTAG-N-BlastsTagRFP-TUBA1B repair plasmid (Addgene # 207768). ..

    Article Title: Modular engineering of thermoresponsive allosteric proteins.
    Article Snippet: The Renilla luciferase expression plasmid was purchased from Promega. .. H2B–GFP was a gift from G. Wahl (Addgene, plasmid 11680). pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA was a gift from F. Zhang (Addgene, plasmid 61591) and sgRNA1_Tet-inducible luciferase reporter and Tet-inducible mCherry reporter were gifts from M. Sato (Addgene, plasmids 64161 and 64128). ..

    Article Title: Periodic confined cell migration drives partially reversible chromatin reorganization in cancer cell lines
    Article Snippet: .. Both cell types were transfected with a plasmid encoding the H2B-GFP fusion protein from Geoff Wahl (Addgene plasmid #11680) to generate a stable cell line constitutively expressing human histone H2B, one of the core proteins in DNA packaging, tagged with Green Fluorescent Protein (GFP). ..

    Article Title: Intensity modulation of trichromatic split fluorescent proteins for live cell mapping.
    Article Snippet: The following cDNAs were synthesized with optimized codons by GeneArt (Thermo Fisher Scientific, Waltham, MA): sfGFP1-10, 3 CFP21-10, 39 mNeonGreen3A1-10, 11 sfCherry3Csp1-10, mRuby41-10, 10 GB1,18 ΔSUMOstar.19 Additional split fluorescent protein variants were generated through site-directed mutagenesis of existing templates: Cerulean1-10 10 and YFP11-10 39 from sfGFP1-10, mNeonGreen3K1-10 11 from mNeonGreen3A1-10, and sfCherry3C1-10 12 from sfCherry21-10 (Addgene: #82603). .. The Histone H2B coding sequence was subcloned from Addgene (plasmid # 11680 for Histone H2B; Cambridge, MA). .. Vector construction for stable cell line generation A base Tol2 transposon vector (pT2A-IRES-iRFP670) was constructed by subcloning iRFP670 cDNA40 with an internal ribosome entry site (IRES)41 into the pT2A vector.37 CFP21-10 cDNA was subsequently PCR-amplified and inserted into this base vector using InFusion assembly (Clontech, Mountain View, CA) to generate pT2A_CFP21-10-IRES-iRFP670.

    Article Title: Periodic confined cell migration drives partially reversible chromatin reorganization in cancer cell lines.
    Article Snippet: .. Both cell types were transfected with a plasmid encoding the H2B-GFP fusion protein from Geoff Wahl (Addgene plasmid #11680) to generate a stable cell line constitutively expressing human histone H2B, one of the core proteins in DNA packaging, tagged with Green Fluorescent Protein (GFP). ..

    Article Title: Atypical fusion among non-malignant cells: Unveiling the link between post-fusion-induced genome chaos and cancer initiation.
    Article Snippet: The following supplements were further added to the culture medium: 10 μg/mL recombinant human epidermal growth factor (rhEGF), 5 μg/mL human recombinant insulin, 0.5 μg/mL hydrocortisone, 4 μg/mL human transferrin and 10 nM β-estrogen (all supplements were purchased from Merck KGaA, Darmstadt, Germany). .. Histone labeling of M13SV1 cells was performed with the following vectors: H2B-GFP, provided by Geoff Wahl (Addgene plasmid # 11680; h t t p : / / n 2 t . n e t / a d d g e n e : 1 1 6 8 0 ; RRID: Addgene_11680)) [39]; pH2B-mCherryIRES-puro2, a gift from Daniel Gerlich (Addgene plasmid # 21045; http://n2t.net/addgene:21045; RRID: Addgene_21045) [40]; and pELF1a-H2B-GFP-IRES-Hyg. .. The vector pELF1a-H2B-GFP-IRES-HygR was generated via the In-Fusion HD Cloning Kit (Takara, SaintGermain-en-Laye, France).

    Luciferase:

    Article Title: Modular engineering of thermoresponsive allosteric proteins.
    Article Snippet: The Renilla luciferase expression plasmid was purchased from Promega. .. H2B–GFP was a gift from G. Wahl (Addgene, plasmid 11680). pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA was a gift from F. Zhang (Addgene, plasmid 61591) and sgRNA1_Tet-inducible luciferase reporter and Tet-inducible mCherry reporter were gifts from M. Sato (Addgene, plasmids 64161 and 64128). ..

    Transfection:

    Article Title: Periodic confined cell migration drives partially reversible chromatin reorganization in cancer cell lines
    Article Snippet: .. Both cell types were transfected with a plasmid encoding the H2B-GFP fusion protein from Geoff Wahl (Addgene plasmid #11680) to generate a stable cell line constitutively expressing human histone H2B, one of the core proteins in DNA packaging, tagged with Green Fluorescent Protein (GFP). ..

    Article Title: Periodic confined cell migration drives partially reversible chromatin reorganization in cancer cell lines.
    Article Snippet: .. Both cell types were transfected with a plasmid encoding the H2B-GFP fusion protein from Geoff Wahl (Addgene plasmid #11680) to generate a stable cell line constitutively expressing human histone H2B, one of the core proteins in DNA packaging, tagged with Green Fluorescent Protein (GFP). ..

    other:

    Article Title: Distinct cMET inhibitors uncover pharmacological heterogeneity in SHH medulloblastoma cell lines
    Article Snippet: ON-TARGET SMARTpool siRNA against c-MET (Cat.ID: L-003156-00-0005) and siGENOME non-Targeting siRNA Pool (Cat.ID: L-001810-01-05) were purchased from Dharmacon and resuspended in DNase/RNase-free water at 20 μM stock concentration.

    Sequencing:

    Article Title: Intensity modulation of trichromatic split fluorescent proteins for live cell mapping.
    Article Snippet: The following cDNAs were synthesized with optimized codons by GeneArt (Thermo Fisher Scientific, Waltham, MA): sfGFP1-10, 3 CFP21-10, 39 mNeonGreen3A1-10, 11 sfCherry3Csp1-10, mRuby41-10, 10 GB1,18 ΔSUMOstar.19 Additional split fluorescent protein variants were generated through site-directed mutagenesis of existing templates: Cerulean1-10 10 and YFP11-10 39 from sfGFP1-10, mNeonGreen3K1-10 11 from mNeonGreen3A1-10, and sfCherry3C1-10 12 from sfCherry21-10 (Addgene: #82603). .. The Histone H2B coding sequence was subcloned from Addgene (plasmid # 11680 for Histone H2B; Cambridge, MA). .. Vector construction for stable cell line generation A base Tol2 transposon vector (pT2A-IRES-iRFP670) was constructed by subcloning iRFP670 cDNA40 with an internal ribosome entry site (IRES)41 into the pT2A vector.37 CFP21-10 cDNA was subsequently PCR-amplified and inserted into this base vector using InFusion assembly (Clontech, Mountain View, CA) to generate pT2A_CFP21-10-IRES-iRFP670.

    Labeling:

    Article Title: Atypical fusion among non-malignant cells: Unveiling the link between post-fusion-induced genome chaos and cancer initiation.
    Article Snippet: The following supplements were further added to the culture medium: 10 μg/mL recombinant human epidermal growth factor (rhEGF), 5 μg/mL human recombinant insulin, 0.5 μg/mL hydrocortisone, 4 μg/mL human transferrin and 10 nM β-estrogen (all supplements were purchased from Merck KGaA, Darmstadt, Germany). .. Histone labeling of M13SV1 cells was performed with the following vectors: H2B-GFP, provided by Geoff Wahl (Addgene plasmid # 11680; h t t p : / / n 2 t . n e t / a d d g e n e : 1 1 6 8 0 ; RRID: Addgene_11680)) [39]; pH2B-mCherryIRES-puro2, a gift from Daniel Gerlich (Addgene plasmid # 21045; http://n2t.net/addgene:21045; RRID: Addgene_21045) [40]; and pELF1a-H2B-GFP-IRES-Hyg. .. The vector pELF1a-H2B-GFP-IRES-HygR was generated via the In-Fusion HD Cloning Kit (Takara, SaintGermain-en-Laye, France).



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